Accurate PCR detection of influenza A/B and respiratory syncytial viruses by use of Cepheid Xpert Flu+RSV Xpress Assay in point-of-care settings: Comparison to Prodesse ProFlu+

ABSTRACT The Xpert Flu+RSV Xpress Assay is a fast, automated in vitro diagnostic test for qualitative detection and differentiation of influenza A and B viruses and respiratory syncytial virus (RSV) performed on the Cepheid GeneXpert Xpress System. The objective of this study was to establish performance characteristics of the Xpert Flu+RSV Xpress Assay compared to those of the Prodesse ProFlu+ real-time reverse transcription-PCR (RT-PCR) assay (ProFlu+) for the detection of influenza A and B viruses as well as RSV in a Clinical Laboratory Improvement Amendments (CLIA)-waived (CW) setting. Overall, the assay, using fresh and frozen nasopharyngeal (NP) swabs, demonstrated high concordance with results of the ProFlu+ assay in the combined CW and non-CW settings with positive percent agreements (PPA) (100%, 100%, and 97.1%) and negative percent agreements (NPA) (95.2%, 99.5%, and 99.6%) for influenza A and B viruses and RSV, respectively. In conclusion, this multicenter study using the Cepheid Xpert Flu+RSV Xpress Assay demonstrated high sensitivities and specificities for influenza A and B viruses and RSV in ∼60 min for use at the point-of-care in the CW setting. </jats:p

Multidrug transporter MRP4/ABCC4 as a key determinant of pancreatic cancer aggressiveness

Recent findings show that MRP4 is critical for pancreatic ductal adenocarcinoma (PDAC) cell proliferation. Nevertheless, the significance of MRP4 protein levels and function in PDAC progression is still unclear. The aim of this study was to determine the role of MRP4 in PDAC tumor aggressiveness. Bioinformatic studies revealed that PDAC samples show higher MRP4 transcript levels compared to normal adjacent pancreatic tissue and circulating tumor cells express higher levels of MRP4 than primary tumors. Also, high levels of MRP4 are typical of high-grade PDAC cell lines and associate with an epithelial-mesenchymal phenotype. Moreover, PDAC patients with high levels of MRP4 depict dysregulation of pathways associated with migration, chemotaxis and cell adhesion. Silencing MRP4 in PANC1 cells reduced tumorigenicity and tumor growth and impaired cell migration. Transcriptomic analysis revealed that MRP4 silencing alters PANC1 gene expression, mainly dysregulating pathways related to cell-to-cell interactions and focal adhesion. Contrarily, MRP4 overexpression significantly increased BxPC-3 growth rate, produced a switch in the expression of EMT markers, and enhanced experimental metastatic incidence. Altogether, our results indicate that MRP4 is associated with a more aggressive phenotype in PDAC, boosting pancreatic tumorigenesis and metastatic capacity, which could finally determine a fast tumor progression in PDAC patients.Fil: Sahores, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Carozzo, A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: May, M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Gomez, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Di Siervi, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: de Sousa Serro, Maximiliano Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Yaneff, Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Rodriguez Gonzalez, Angela Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Abba, Martín Carlos. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Shayo, Carina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Davio, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentin

Body Composition and Anthropometric Changes During a 10-week Training Academy in Police Recruits

Obesity and cardiometabolic risk factors are often present in law enforcement personnel, which may compromise physical readiness and long-term health. As such, physical fitness interventions are warranted for promoting officers\u27 performance and wellbeing. PURPOSE: To determine the body composition and anthropometric changes experienced by police recruits undergoing a departmental training academy. METHODS: Twenty-one police recruits (20 M, 1 F; age: 25.1 ± 5.0 y; BMI: 27.8 ± 4.3 kg/m2) were tested before and after a 10-week training academy in Lubbock, Texas. Supervised physical training was conducted 5 times per week and consisted of ~1–1.5 hours of high-intensity, multi-modal (i.e., running, weightlifting, calisthenics), functional training following linear periodization. Dual-energy X-ray absorptiometry (DXA; GE Lunar iDXA) and 3-dimensional optical imaging (3DO; Size Stream SS20) were performed to assess body composition and anthropometry. Paired-samples t-tests were performed to compare values before and after the training academy, and Cohen’s d effect sizes were generated. After Bonferroni correction, statistical significance was accepted at p\u3c0.003. Changes are presented as mean ± SD. RESULTS: From DXA, statistically significant decreases in total fat mass (FM; -3.3 ± 3.1 kg, p\u3c0.001, d=1.1), trunk FM (-2.1 ± 2.2 kg, p\u3c0.001, d=1.0), arms FM (-0.3 ± 0.3 kg, p=0.001, d=1.1), legs FM (-0.9 ± 0.9 kg, p\u3c0.001, d=1.1), and body fat percentage (-3.1 ± 2.5%, p\u3c0.001, d=1.2) were observed. Increases in total lean soft tissue (LST; 1.3 ± 1.3 kg, p=0.002, d=1.0) and trunk LST (0.8 ± 0.9 kg, p\u3c0.001, d=0.9) were also noted, with trends for increases in leg LST (0.2 ± 0.7 kg, p=0.096, d=0.4) and arm LST (0.2 ± 0.4, p=0.04, d=0.5). Decreases in 3DO abdomen circumference (-3.5 ± 3.8 cm, p\u3c0.001, d=0.9) and hip circumference (-2.2 ± 2.2 cm, p\u3c0.001, d=1.0) were noted, with trends for decreases in the circumferences of the waist (-2.4 ± 3.6 cm, p=0.007, d=0.7) and upper arm (-0.9 ± 1.5 cm, p=0.02, d=0.6). No significant changes in thigh circumference (-0.7 ± 1.9 cm, p=0.12, d=0.4) or calf circumference (-0.2 ± 1.5 cm, p=0.52, d=0.1) were noted. A trend for a decrease in body mass (-2.0 ± 3.1 kg, p=0.007, d=0.7) was also observed. CONCLUSION: The present study indicates that police academy training significantly improves recruits\u27 body composition, both reducing FM and increasing LST, which has the potential to positively affect operational performance. Future studies should track these changes over time to help develop ongoing health and fitness strategies for career police officers, ultimately improving their long-term wellbeing and job readiness

Adherence to european clinical practice guidelines for secondary prevention of cardiovascular disease : A cohort study

Funding: This study was supported by a grant from the Primary Health Care University Research Institute (IDIAP) Jordi Gol located in Barcelona, Spain (Grant ref: IDIAP 7Z12/006).To provide a better understanding of the actions taken within health systems and their results, this study aims to assess clinicians' adherence to clinical practice guidelines regarding recommended treatments in patients with cardiovascular disease in primary care settings, and to determine the associated factors. We conducted an ambispective cohort study in 21 primary care centres in 8 Spanish regions. Patients diagnosed with coronary heart disease, stroke and/or peripheral arterial disease were included. Patients who received the treatment recommended in the European guidelines on cardiovascular disease prevention (CPG's adherent group) were compared with patients who did not (CPG's non-adherent group). The outcome variables were cardiovascular hospital admissions, all-cause and cardiovascular mortality during follow-up. Of the 438 participants, 38.6% (n = 169) received the drug therapies recommended in the guidelines. The factors that increased the likelihood of good adherence to CPG's were being diagnosed with hypertension (p = 0.001), dyslipidaemia (p < 0.001) or diabetes (p = 0.001), and not having a psychiatric disorder (p = 0.005). We found no statistically significant association between good adherence to CPG's and lower incidence of events (p = 0.853). Clinician adherence to guidelines for secondary prevention of cardiovascular disease was low in the primary care setting

Risk of COVID-19 after natural infection or vaccination

BACKGROUND: While vaccines have established utility against COVID-19, phase 3 efficacy studies have generally not comprehensively evaluated protection provided by previous infection or hybrid immunity (previous infection plus vaccination). Individual patient data from US government-supported harmonized vaccine trials provide an unprecedented sample population to address this issue. We characterized the protective efficacy of previous SARS-CoV-2 infection and hybrid immunity against COVID-19 early in the pandemic over three-to six-month follow-up and compared with vaccine-associated protection. METHODS: In this post-hoc cross-protocol analysis of the Moderna, AstraZeneca, Janssen, and Novavax COVID-19 vaccine clinical trials, we allocated participants into four groups based on previous-infection status at enrolment and treatment: no previous infection/placebo; previous infection/placebo; no previous infection/vaccine; and previous infection/vaccine. The main outcome was RT-PCR-confirmed COVID-19 \u3e7-15 days (per original protocols) after final study injection. We calculated crude and adjusted efficacy measures. FINDINGS: Previous infection/placebo participants had a 92% decreased risk of future COVID-19 compared to no previous infection/placebo participants (overall hazard ratio [HR] ratio: 0.08; 95% CI: 0.05-0.13). Among single-dose Janssen participants, hybrid immunity conferred greater protection than vaccine alone (HR: 0.03; 95% CI: 0.01-0.10). Too few infections were observed to draw statistical inferences comparing hybrid immunity to vaccine alone for other trials. Vaccination, previous infection, and hybrid immunity all provided near-complete protection against severe disease. INTERPRETATION: Previous infection, any hybrid immunity, and two-dose vaccination all provided substantial protection against symptomatic and severe COVID-19 through the early Delta period. Thus, as a surrogate for natural infection, vaccination remains the safest approach to protection. FUNDING: National Institutes of Health

Estudio aerobiológico de la diversidad polínica y su potencial alergénico en el oasis del sur de Mendoza, Argentina

Los conocimientos provenientes del campo de estudio de la Aerobiología favorecen el análisis inmunológico de los alérgenos atmosféricos procedentes de polen y esporas fúngicas. Esto posibilita conocer la carga alergénica del aire en el ambiente y de esta manera, valorar mejor la relación exposición / reacción / clínica en los pacientes en tratamiento por alergias. No existen estudios previos de este tema realizados a nivel regional ni provincial en Mendoza. Una base de datos de identificación de posibles alérgenos provenientes de la polinización de espacios verdes urbanos en la ciudad de San Rafael y General Alvear contribuye a la epidemiología ambiental sobre las afecciones alérgicas respiratorias inducidas por polen y esporas. En esta presentación damos a conocer un proyecto de investigación en Aerobiología, con el fin de generar conocimiento aerobiológico de la zona urbana del oasis del sur mendocino (San Rafael y General Alvear), que contribuye a conocer la carga alergénica proveniente de granos de polen y esporas presentes en el ambiente. Para ello, se están llevando a cabo tres líneas de trabajo que consisten en: (1) el relevamiento, localización y mapeo de la vegetación urbana en floración, (2) la elaboración de una colección de referencia palinológica, y (3) el muestreo diario de aeropartículas atmosféricas urbanas. Se presentan los resultados preliminares obtenidos desde el inicio del proyecto y se muestran las líneas de trabajo que seguirá el curso de esta investigación. A futuro, los estudios aerobiológicos permitirían el desarrollo de programas de seguimiento, prevención y control en los índices de la cantidad de polen y esporas presentes en la atmósfera. Esta herramienta puede describir el potencial alergénico en espacios urbanos sus perjuicios ambientales. De esta manera, una investigación con estas características puede ser un aporte directo a la formulación de políticas de salud pública y planificación urbana de la ciudad.Fil: Guerci, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Museo Municipal de Historia Natural San Rafael - Unidad Asociada al CCT Mendoza; Argentina. Instituto de Enseñanza Superior 9-011 del Atuel; ArgentinaFil: Rojo, Leandro David. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Museo Municipal de Historia Natural San Rafael - Unidad Asociada al CCT Mendoza; ArgentinaFil: Indiveri, Martina. Gobierno de la Provincia de Mendoza. Hospital Teodoro Schestakow.; ArgentinaFil: Nuñez Sada, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Farina, Lucia. Museo Municipal de Historia Natural San Rafael - Unidad Asociada al CCT Mendoza; ArgentinaFil: Aguilar, Mariano. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Llano, Carina Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Mendoza; ArgentinaFil: Lucero, A.. Universidad de Mendoza; ArgentinaFil: Negreira, Gabriel Alfredo. Instituto de Enseñanza Superior 9-011 del Atuel; ArgentinaFil: Vazquez, Maria Soledad. Universidad Tecnologica Nacional. Facultad Reg.san Rafael. Instituto de Evolucion, Ecologia Historica y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mendoza. Instituto de Evolucion, Ecologia Historica y Ambiente.; ArgentinaFil: Rodriguez, L. F.. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Gallardo, C. A.. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Giraudo, S. B.. Museo Municipal de Historia Natural San Rafael - Unidad Asociada al CCT Mendoza; ArgentinaXIV Encuentro del Centro Internacional de Ciencias de la TierraSan RafaelArgentinaCentro Internacional para Estudios de la TierraComisión Nacional de Energía AtómicaUniversidad Nacional de CuyoUniversidad Tecnológica Nacional. Facultad Regional San Rafae

Family History and Breast Cancer Hormone Receptor Status in a Spanish Cohort

Breast cancer is a heterogenous disease that impacts racial/ethnic groups differently. Differences in genetic composition, lifestyles, reproductive factors, or environmental exposures may contribute to the differential presentation of breast cancer among Hispanic women.A population-based study was conducted in the city of Santiago de Compostela, Spain. A total of 645 women diagnosed with operable invasive breast cancer between 1992 and 2005 participated in the study. Data on demographics, breast cancer risk factors, and clinico-pathological characteristics of the tumors were collected. Hormone receptor negative tumors were compared with hormone receptor postive tumors on their clinico-pathological characteristics as well as risk factor profiles.Among the 645 breast cancer patients, 78% were estrogen receptor-positive (ER+) or progesterone receptor-positive (PR+), and 22% were ER−&PR−. Women with a family history of breast cancer were more likely to have ER−&PR− tumors than women without a family history (Odds ratio, 1.43; 95% confidence interval, 0.91–2.26). This association was limited to cancers diagnosed before age 50 (Odds ratio, 2.79; 95% confidence interval, 1.34–5.81).An increased proportion of ER−&PR− breast cancer was observed among younger Spanish women with a family history of the disease

Radial velocity confirmation of K2-100b: A young, highly irradiated, and low-density transiting hot Neptune

We present a detailed analysis of HARPS-N radial velocity observations of K2-100, a young and active star in the Praesepe cluster, which hosts a transiting planet with a period of 1.7 d. We model the activity-induced radial velocity variations of the host star with a multidimensional Gaussian Process framework and detect a planetary signal of 10.6 \ub1 3.0 m s−1, which matches the transit ephemeris, and translates to a planet mass of 21.8 \ub1 6.2 M. We perform a suite of validation tests to confirm that our detected signal is genuine. This is the first mass measurement for a transiting planet in a young open cluster. The relatively low density of the planet, 2.04+−006661 g cm−3, implies that K2-100b retains a significant volatile envelope. We estimate that the planet is losing its atmosphere at a rate of 1011–1012 g s−1 due to the high level of radiation it receives from its host star

TOI-1130: A photodynamical analysis of a hot Jupiter in resonance with an inner low-mass planet

The TOI-1130 is a known planetary system around a K-dwarf consisting of a gas giant planet, TOI-1130 c on an 8.4-day orbit that is accompanied by an inner Neptune-sized planet, TOI-1130 b, with an orbital period of 4.1 days. We collected precise radial velocity (RV) measurements of TOI-1130 with the HARPS and PFS spectrographs as part of our ongoing RV follow-up program. We performed a photodynamical modeling of the HARPS and PFS RVs, along with transit photometry from the Transiting Exoplanet Survey Satellite (TESS) and the TESS Follow-up Observing Program (TFOP). We determined the planet masses and radii of TOI-1130 b and TOI-1130 c to be Mb = 19.28 \ub1 0.97M⊕ and Rb = 3.56 \ub1 0.13 R⊕, and Mc = 325.59 \ub1 5.59M⊕ and Rc = 13.32-1.41+1.55 R⊕, respectively. We have spectroscopically confirmed the existence of TOI-1130 b, which had previously only been validated. We find that the two planets have orbits with small eccentricities in a 2:1 resonant configuration. This is the first known system with a hot Jupiter and an inner lower mass planet locked in a mean-motion resonance. TOI-1130 belongs to the small, yet growing population of hot Jupiters with an inner low-mass planet that poses a challenge to the pathway scenario for hot Jupiter formation. We also detected a linear RV trend that is possibly due to the presence of an outer massive companion

TOI-969: a late-K dwarf with a hot mini-Neptune in the desert and an eccentric cold Jupiter

Context. The current architecture of a given multi-planetary system is a key fingerprint of its past formation and dynamical evolution history. Long-term follow-up observations are key to complete their picture. Aims. In this paper, we focus on the confirmation and characterization of the components of the TOI-969 planetary system, where TESS detected a Neptune-size planet candidate in a very close-in orbit around a late K-dwarf star. Methods. We use a set of precise radial velocity observations from HARPS, PFS, and CORALIE instruments covering more than two years in combination with the TESS photometric light curve and other ground-based follow-up observations to confirm and characterize the components of this planetary system. Results. We find that TOI-969 b is a transiting close-in (Pb ∼ 1.82 days) mini-Neptune planet (Formula Presented), placing it on the lower boundary of the hot-Neptune desert (Teq,b = 941 \ub1 31 K). The analysis of its internal structure shows that TOI-969 b is a volatile-rich planet, suggesting it underwent an inward migration. The radial velocity model also favors the presence of a second massive body in the system, TOI-969 c, with a long period of (Formula Presented) days, a minimum mass of (Formula Presented), and a highly eccentric orbit of (Formula Presented). Conclusions. The TOI-969 planetary system is one of the few around K-dwarfs known to have this extended configuration going from a very close-in planet to a wide-separation gaseous giant. TOI-969 b has a transmission spectroscopy metric of 93 and orbits a moderately bright (G = 11.3 mag) star, making it an excellent target for atmospheric studies. The architecture of this planetary system can also provide valuable information about migration and formation of planetary systems