Base composition of intact nucleic acid oligomers

Base composition of intact nucleic acid oligomer

The Yamabe invariant for axially symmetric two Kerr black holes initial data

An explicit 3-dimensional Riemannian metric is constructed which can be interpreted as the (conformal) sum of two Kerr black holes with aligned angular momentum. When the separation distance between them is large we prove that this metric has positive Ricci scalar and hence positive Yamabe invariant. This metric can be used to construct axially symmetric initial data for two Kerr black holes with large angular momentum.Comment: 14 pages, 2 figure

Midwinter suppression of baroclinic storm activity on Mars: observations and models

Baroclinic instability and intense traveling wave activity on Mars is well known to occur in “storm zones” (Hollingsworth et al. 1996) close to the edge of the advancing or retreating polar ice cap. Such activity usually sets in during Martian fall and continues until the onset of the summer season when large-scale instability mostly ceases as the atmosphere is no longer baroclinically unstable. The stormy season is typically characterized by large-scale, zonally-propagating waves with zonal wavenumbers m = 1-3, the lower wavenumber modes typically penetrating to considerable altitude though may also be surface-intensified. As we show below, however, some observations suggest that this eddy activity does not persist uniformly throughout the autumn, winter and spring seasons, but appears to die down quite consistently within 10 sols or so either side of the winter solstice. This midwinter ‘solsticial pause’ appears to be a sufficiently consistent feature of each winter season in both hemispheres to be regarded as a significant feature of Martian climatology, and could affect a variety of aspects of Martian meteorology including global heat and momentum transport, occurrence of dust storms etc. A somewhat similar phenomenon has also been documented for the Earth (e.g. Nakamura 1992; Penny et al. 2010), especially in relation to seasonal variations in the north Pacific storm tracks. The cause of this phenomenon is still not well established, though suggested mechanisms include the effects of enhanced barotropic shear (the so-called ‘barotropic governor’ (James & Gray 1986) and interactions with topography over central Asia. In this presentation we examine evidence for this phenomenon in the assimilated record of Martian climate from the Thermal Emission Spectrometer on board the Mars Global Surveyor mission (MGSTES), in conjunction with the UK version of the LMD-Oxford-OU-IAA Mars GCM (Forget et al. 1999; Montabone et al. 2006; Lewis et al. 2007). This is further corroborated in other evidence from seasonal variations in the incidence of local and regional dust storms that owe their origin to circumpolar baroclinic storms. We also discuss the extent to which this ‘solsticial pause’ phenomenon is reproduced in stand-alone atmospheric models and present results of some simulations to test a number of hypotheses for its dynamical origin on Mars

Endosome pH measured in single cells by dual fluorescence flow cytometry: rapid acidification of insulin to pH 6

The acidification of various ligands was measured on a cell by cell basis for cell suspensions by correlated dual fluorescence flow cytometry. Mouse 3T3 cells were incubated with a mixture of fluorescein- and rhodamine-conjugated ligands, and the ratio of fluorescein and rhodamine fluorescence was used as a measure of endosome pH. The calibration of this ratio by both fluorometry and flow cytometry is described. Dual parameter histograms of average endosome pH per cell versus amount of internalization were calculated from this data, for samples in the absence and presence of chloroquine added to neutralize acidic cellular vesicles. The kinetics of acidification of insulin were measured and compared with previous results obtained with the chloroquine ratio technique. Rapid acidification of internalized ligand was observed both for insulin, which was mostly internalized via nonspecific pathways, and for alpha 2-macroglobulin, which was mainly internalized by specific receptor-mediated endocytosis. The average pH observed for internalized insulin was less than pH 6 within 10 min after addition of insulin. At 30 min, the average pH began to decrease to approximately pH 5, presumably because of fusion of endosomes with lysosomes

Gating-by-tilt of mechanosensitive membrane channels

We propose an alternative mechanism for the gating of biological membrane channels in response to membrane tension that involves a change in the slope of the membrane near the channel. Under biological membrane tensions we show that the energy difference between the closed (tilted) and open (untilted) states can far exceed kBT and is comparable to what is available under simple ilational gating. Recent experiments demonstrate that membrane leaflet asymmetries (spontaneous curvature) can strong effect the gating of some channels. Such a phenomenon would be more easy to explain under gating-by-tilt, given its novel intrinsic sensitivity to such asymmetry.Comment: 10 pages, 2 figure

Microscopic formulation of the Zimm-Bragg model for the helix-coil transition

A microscopic spin model is proposed for the phenomenological Zimm-Bragg model for the helix-coil transition in biopolymers. This model is shown to provide the same thermophysical properties of the original Zimm-Bragg model and it allows a very convenient framework to compute statistical quantities. Physical origins of this spin model are made transparent by an exact mapping into a one-dimensional Ising model with an external field. However, the dependence on temperature of the reduced external field turns out to differ from the standard one-dimensional Ising model and hence it gives rise to different thermophysical properties, despite the exact mapping connecting them. We discuss how this point has been frequently overlooked in the recent literature.Comment: 11 pages, 2 figure

Reachability in Parametric Interval Markov Chains using Constraints

Parametric Interval Markov Chains (pIMCs) are a specification formalism that extend Markov Chains (MCs) and Interval Markov Chains (IMCs) by taking into account imprecision in the transition probability values: transitions in pIMCs are labeled with parametric intervals of probabilities. In this work, we study the difference between pIMCs and other Markov Chain abstractions models and investigate the two usual semantics for IMCs: once-and-for-all and at-every-step. In particular, we prove that both semantics agree on the maximal/minimal reachability probabilities of a given IMC. We then investigate solutions to several parameter synthesis problems in the context of pIMCs -- consistency, qualitative reachability and quantitative reachability -- that rely on constraint encodings. Finally, we propose a prototype implementation of our constraint encodings with promising results

On the existence of initial data containing isolated black holes

We present a general construction of initial data for Einstein's equations containing an arbitrary number of black holes, each of which is instantaneously in equilibrium. Each black hole is taken to be a marginally trapped surface and plays the role of the inner boundary of the Cauchy surface. The black hole is taken to be instantaneously isolated if its outgoing null rays are shear-free. Starting from the choice of a conformal metric and the freely specifiable part of the extrinsic curvature in the bulk, we give a prescription for choosing the shape of the inner boundaries and the boundary conditions that must be imposed there. We show rigorously that with these choices, the resulting non-linear elliptic system always admits solutions.Comment: 11 pages, 2 figures, RevTeX

Evaluation of positive G sub Z tolerance following simulated weightlessness (bedrest)

The magnitude of physiologic changes which are known to occur in human subjects exposed to varying levels of + G sub Z acceleration following bed rest simulation of weightlessness was studied. Bed rest effects were documented by fluid and electrolyte balance studies, maximal exercise capability, 70 deg passive tilt and lower body negative pressure tests and the ability to endure randomly prescribed acceleration profiles of +2G sub Z, +3G sub Z, and +4G sub Z. Six healthy male volunteers were studied during two weeks of bed rest after adequate control observations, followed by two weeks of recovery, followed by a second two-week period of bed rest at which time an Air Force cutaway anti-G suit was used to determine its effectiveness as a countermeasure for observed cardiovascular changes during acceleration. Results showed uniform and significant changes in all measured parameters as a consequence of bed rest including a reduced ability to tolerate +G sub Z acceleration. The use of anti-G suits significantly improved subject tolerance to all G exposures and returned measured parameters such as heart rate and blood pressure towards or to pre-bed-rest (control) values in four of the six cases

The Role of Patient Activation in Preferences for Shared Decision Making: Results From a National Survey of US Adults

Financial support for this study was provided by a contract with UnitedHealthcare, Optum Institute. The funding agreement ensured our independence in designing the study, interpreting the data, and writing and publishing the report. Samuel G. Smith is supported by a Cancer Research UK Postdoctoral Fellowship (C42785=A17965). Carol J. Simon and Steven R. Rush are employed by the sponsor