The Evaluation of the Effects of Different Patient Information Strategy on Self-Performed Oral Hygiene in a Group of Turkish Population

The aim of this study was to investigate the effects of different patient information strategy on self-performed oral hygiene in a short and intermediate time period in a group of Turkish population. The study population consisted of 105 patient at the same socio-economical level and divided into three groups: Group I, who were not attended any program, group II, who attended only one explanatory session about oral hygiene, and group III, who attended a comprehensive education and motivation session. In order to evaluate the methodology applied, the Quigley-IIcin's plaque index (PI, according to Quigley-1 Icin, 1962) and gingival bleeding index (GI, according to Loc and Silness, 1963) were calculated. All these patients' teeth were scaled at the start of the study. These parameters were determined at die initial and after 1st week, 1st and 3rd and 6th months. The results demonstrated a significant improvement in the PI and GI scores following die use of motivation and education program in the 1st week and 1st month (p<0.01). The level of oral hygiene and gingival health achieved at the fist month was not observed in all 3 groups in the 3rd and 6th months. We found that the comprehensive session used here has also been effective in improving the oral hygiene and reducing the gingival inflammation in the 6" month (p<0.01). In conclusion, the data presented in this paper indicated that the education and motivation program with visual and demonstrated may be suitable alternative program improving the oral hygiene and reducing the gingival inflammation.The aim of this study was to investigate the effects of different patient information strategy on self-performed oral hygiene in a short and intermediate time period in a group of Turkish population. The study population consisted of 105 patient at the same socio-economical level and divided into three groups: Group I, who were not attended any program, group II, who attended only one explanatory session about oral hygiene, and group III, who attended a comprehensive education and motivation session. In order to evaluate the methodology applied, the Quigley-IIcin's plaque index (PI, according to Quigley-1 Icin, 1962) and gingival bleeding index (GI, according to Loc and Silness, 1963) were calculated. All these patients' teeth were scaled at the start of the study. These parameters were determined at die initial and after 1st week, 1st and 3rd and 6th months. The results demonstrated a significant improvement in the PI and GI scores following die use of motivation and education program in the 1st week and 1st month (p<0.01). The level of oral hygiene and gingival health achieved at the fist month was not observed in all 3 groups in the 3rd and 6th months. We found that the comprehensive session used here has also been effective in improving the oral hygiene and reducing the gingival inflammation in the 6" month (p<0.01). In conclusion, the data presented in this paper indicated that the education and motivation program with visual and demonstrated may be suitable alternative program improving the oral hygiene and reducing the gingival inflammation

Cloning, expression and characterization of L-arabinose isomerise from thermophilic Anoxybacillus kestanbolensis AC26Sari strain: Bioconversation of L-arabinose to L-ribulose

343-350L-Arabinose isomerase (L-AI) is a pivotal enzyme in the microbial pentose phosphate pathway. It is considered as a significant biological catalyst in rare sugar production. This enzyme can isomerize L-arabinose into L-ribulose and also D-galactose into D-tagatose. Here, we cloned the araA gene encoding L-arabinose isomerase from Anoxybacillus kestanbolensis AC26Sari strain, sequenced and over-expressed in E. coli BL21 (DE3): pLysS. This gene is involved in L-arabinose operon in A. kestanbolensis AC26Sari. DNA sequence analysis revealed an open reading frame of 1,506 bp, capable of encoding a polypeptide of 502 amino acid residues with calculated molecular weight of 55.6776 kDa. The recombinant was purified by heat treatment and Ni-HisTaq chromatography. The purified enzyme showed maximal activity at pH 8.5 and 65ºC and required divalent cations such as Co2+ and Mn2+ for its activity and thermostability. The apparent Km value of the enzyme for L-arabinose was 6.5 mM (Vmax, 140.1002 U/mg) as determined in the precence of both 1 mM Co2+ and Mn2+

Point-of-Care Emergency Ultrasonography in Non-Traumatic Cardiac Arrest and Near-Arrest Emergency Patients; A Pilot Trial

Background: In this study, we evaluated the applicability and interpretation of point-of-care emergency ultrasound (POCEUS) performed by an emergency physician (EP) in non-traumatic adult cardiac arrest and near-arrest patients at presentation to the Emergency Department (ED).   Methods: POCEUS was performed in 5 steps on 73 adults to assess; 1. Qualitative global cardiac function, cardiac chambers and presence of pericardial effusion; 2. Presence of pleural sliding, B-lines, A-lines or consolidation on anterior-superior; 3. Presence of an abdominal aorta aneurysm and pelvic free fluid; 4. Presence of pleural effusion, consolidation, free fluid on lateral-inferior; 5. Qualitative width and collapsibility of the inferior vena cava. A fulfilled checklist and real-time images of ultrasonography were sent by WhatsApp to the head of the study to generate the evidence and collect the data. The process of patient care, in-hospital diagnosis and survival were retrieved from digital hospital records. This prospective multicenter sample study was conducted from November 16, 2015, to January 5, 2016.   Results: The most common findings of POCEUS were performed and interpreted to have a first prediction of patients’ acute clinic problem by EPs were compatible with global systolic dysfunction (n = 16, 22.9%), pulmonary edema (n = 17, 23.3%), pulmonary embolus (n = 6, 8.2%), distributive/hypovolemic shock (n = 12, 16.4%), cardiac tamponade or pericardial effusion (n = 5, 6.8%), and pneumonia (n = 31, 42.5%) at presentation. The kappa correlation coefficient value of the POCEUS at presentation versus the final, traditional clinical diagnosis of the admitted ward, was 0.773 (95% CI, 0.747–0.892; p = 0.064, McNemar).   Conclusions: POCEUS performed by an EP at presentation had a good agreement between in qualitative prediction of the first differential diagnosis in life-threatened patients and the last diagnosis obtained during hospitalization. Furthermore, this study showed the requirement of evidence in comparison of measurements to the qualitative manner and new descriptive processes in POCEUS for unexplained situations and questions

Molecular analysis of the genus Anoxybacillus based on sequence similarity of the genes recN, flaA, and ftsY

Genome predictions based on selected genes would be a very welcome approach for taxonomic studies. We analyzed three genes, recN, flaA, and ftsY, for determining if these genes are useful tools for systematic analyses in the genus Anoxybacillus. The genes encoding a DNA repair and genetic recombination protein (recN), the flagellin protein (flaA), and GTPase signal docking protein (ftsY) were sequenced for ten Anoxybacillus species. The sequence comparisons revealed that recN sequence similarities range between 61% and 99% in the genus Anoxybacillus. Comparisons to other bacterial recN genes indicated that levels of similarity did not differ from the levels within genus Anoxybacillus. These data showed that recN is not a useful marker for the genus Anoxybacillus. A 550-600-bp region of the flagellin gene was amplified for all Anoxybacillus strains except for Anoxybacillus contaminans. The sequence similarity of flaA gene varies between 61% and 76%. Comparisons to other bacterial flagellin genes obtained from GenBank (Bacillus, Pectinatus, Proteus, and Vibrio) indicated that the levels of similarity were lower (3-42%). Based on these data, we concluded that the variability in this single gene makes it a particularly useful marker. Another housekeeping gene ftsY suggested to reflect the G+C (mol/mol) content of whole genome was analyzed for Anoxybacillus strains. A mean difference of 1.4% was observed between the G+C content of the gene ftsY and the G+C content of the whole genome. These results showed that the gene ftsY can be used to represent whole G+C content of the Anoxybacillus species. © 2012 Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i.Acknowledgments We would like to thank TUBITAK for the support