Phosphorylation of MAP Kinases crucially controls the response to environmental stress in Dunaliella viridis

The green unicellular microalga Dunaliella viridis has the ability to cope with a wide variety of environmental stressful conditions, such as thermal and osmotic shocks, high PAR, UV radiation and nitrogen deficiency. The lack of a rigid cell wall makes D. viridis an excellent model organism to study stress signaling in eukaryotic unicellular organisms. Mitogen-activated protein kinases (MAPKs) are highly conserved serine/threonine kinases that convert extracellular stimuli into a wide range of responses at both cellular and nuclear levels. In eukaryotic cells, MAPKs are involved in both cell proliferation and differentiation (ERK pathway) and stress responses (JNK and p38 pathways), through protein kinase cascades. Significantly lesser phosphorylation levels of ERK-like protein were observed in D. viridis cultures acclimated to high salinity (3-4 M NaCl). In contrast, JNK-like and p38-like proteins phosphorylation levels increased in stressed cells. Likewise, the efficacy of specific commercial inhibitors of the phosphorylation of ERK (PD98059), JNK (SP600125) and p38 (SB203580) revealed the importance of JNK-like proteins in the maintenance of cell viability, the highlighted participation of p38-like proteins and the non-direct implication of the ERK-like proteins in the acclimatization process. In summary, specific blockade of JNK- and p38-like cascades in stressed cells led to rapid cell death. The behavior of MAPK-like proteins in algae is not known in depth, so the analysis of their mechanism of action, as well as their function in this model microalga, will allow to estimate the fate of unicellular eukaryotic organisms in aquatic ecosystems subjected to environmental stress derived from the conditions prevailing within a framework of global climate change.Peer reviewe

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Deep-Sea Bioluminescence Blooms after Dense Water Formation at the Ocean Surface

The deep ocean is the largest and least known ecosystem on Earth. It hosts numerous pelagic organisms, most of which are able to emit light. Here we present a unique data set consisting of a 2.5-year long record of light emission by deep-sea pelagic organisms, measured from December 2007 to June 2010 at the ANTARES underwater neutrino telescope in the deep NW Mediterranean Sea, jointly with synchronous hydrological records. This is the longest continuous time-series of deep-sea bioluminescence ever recorded. Our record reveals several weeks long, seasonal bioluminescence blooms with light intensity up to two orders of magnitude higher than background values, which correlate to changes in the properties of deep waters. Such changes are triggered by the winter cooling and evaporation experienced by the upper ocean layer in the Gulf of Lion that leads to the formation and subsequent sinking of dense water through a process known as “open-sea convection”. It episodically renews the deep water of the study area and conveys fresh organic matter that fuels the deep ecosystems. Luminous bacteria most likely are the main contributors to the observed deep-sea bioluminescence blooms. Our observations demonstrate a consistent and rapid connection between deep open-sea convection and bathypelagic biological activity, as expressed by bioluminescence. In a setting where dense water formation events are likely to decline under global warming scenarios enhancing ocean stratification, in situ observatories become essential as environmental sentinels for the monitoring and understanding of deep-sea ecosystem shifts

Time series of oceanographic parameters measured at the Lacaze-Duthiers Canyon (LDC) and the open-sea convection region in the Gulf of Lion (LION) from January 2008 to June 2010.

<p>(<b>a</b>) Potential temperature at 500 and 1,000 m depth at the LDC mooring site and (<b>b</b>) from various water depths at the LION site, jointly with (<b>c</b>) salinity at 2,300 m depth, (<b>d</b>) horizontal current speed and (<b>e</b>) vertical current speed from various water depths at the LION site. The four levels of temperature measurements at LION presented here are a sub-set of measurement depths (see Fig. S1). Essentially stable temperatures in the deepest layers in 2008 show that open-sea convection reached only 700 m and did not modify the deep water in the study area. In contrast, strong convection events, reaching 2,300 m depth, occurred during February-March 2009 and 2010 with an abrupt cooling of the upper water column and an increase in temperature and salinity in the deep layers. A concurrent increase in current speed was also noticed in winter 2009 and 2010. The 5-month long data gap in 2009 is due to a damaging of the mooring line during the April 2009 recovery, which induced a postponement of its redeployment to September 2009.</p

Links between bioluminescence, current speed and the modification of the properties of the Western Mediterranean Deep Water (WMDW).

<p>Box-and-whisker plot of median PMT counting rates (log scale) versus current speed classes for salinities higher (red) or lower (grey) than 38.479 for data recorded in (<b>a</b>) 2008, (<b>b</b>) 2009 and (<b>c</b>) between January and June 2010. The salinity threshold of 38.479 is used as a marker of the intrusion of newly formed deep water at the ANTARES site. While bioluminescence increases with current speed, it is also enhanced by the modification of WMDW (red box-plots). The top and bottom of each box-plot represent 75% (upper quartile) and 25% (lower quartile) of all values, respectively. The horizontal line is the median. The ends of the whiskers represent the 10^th and 90^th percentiles. Outliers are not represented. The statistical comparison between the two box-plots (red and grey) in each current class is given by the Kruskal-Wallis test: the observed difference between the two samples is significant beyond the 0.05 (*), the 0.01 (**) and the 0.001 (***) levels. The absence of an asterisk in some current classes indicates that the difference between the two box-plots is not significant. The number of measurements for salinity lower or higher than 38.479 is given in black or in red, respectively. Note the different scales of figures a, b and c.</p