Compression of the Left Innominated Vein between the Brachiocephalic Trunk and Left Carotid Artery

AbstractWe present a case of a 25-year-old male who looked for medical attention for symptoms like dysesthesias in his left arm. Physical examination revealed severe dilations of the superficial veins in his left forearm and arm.An ultrasound showed no signs of thrombosis. Dynamic phlebography ruled out the presence of extrinsic compression of the left innominated vein. The angioMRI confirmed that the innominated vein was compressed between the braquiocephalic trunk and left carotid.Therefore, we describe a previously unreported congenital anomaly of the left brachiocephalic vein where the fundamental symptom is the compression of the left innominated trunk

Combined Treatment with Open and Endovascular Surgery of a Mycotic Abdominal Aortic Aneurysm

AbstractWe report the treatment of a 67-year-old male patient with a suprarenal mycotic aortic aneurysm. A 67-year-old male developed renal failure during the treatment of a urine infection and a paracervical abscess. Further investigations revealed a 5.6cm saccular suprarenal abdominal aortic aneurysm. A successful combined treatment was carried out with endovascular techniques and open surgery, realizing a retrograde aorto-superior mesenteric bypass. Postoperative follow up scans showed exclusion and thrombosis of the aneurysm. Treatment of mycotic aneurysms is mainly surgical, but endovascular therapy helps to avoid highly complicated surgery and reduces mortality

Discriminative power of EEG-based biomarkers in major depressive disorder: A systematic review

Currently, the diagnosis of major depressive disorder (MDD) and its subtypes is mainly based on subjective assessments and self-reported measures. However, objective criteria as Electroencephalography (EEG) features would be helpful in detecting depressive states at early stages to prevent the worsening of the symptoms. Scientific community has widely investigated the effectiveness of EEG-based measures to discriminate between depressed and healthy subjects, with the aim to better understand the mechanisms behind the disorder and find biomarkers useful for diagnosis. This work offers a comprehensive review of the extant literature concerning the EEG-based biomarkers for MDD and its subtypes, and identify possible future directions for this line of research. Scopus, PubMed and Web of Science databases were researched following PRISMA’s guidelines. The initial papers’ screening was based on titles and abstracts; then full texts of the identified articles were examined, and a synthesis of findings was developed using tables and thematic analysis. After screening 1871 articles, 76 studies were identified as relevant and included in the systematic review. Reviewed markers include EEG frequency bands power, EEG asymmetry, ERP components, non-linear and functional connectivity measures. Results were discussed in relations to the different EEG measures assessed in the studies. Findings confirmed the effectiveness of those measures in discriminating between healthy and depressed subjects. However, the review highlights that the causal link between EEG measures and depressive subtypes needs to be further investigated and points out that some methodological issues need to be solved to enhance future research in this field

Differential protein expression in peripheral blood mononuclear cells (PBMCs) on patients withd systemic lupus erythematosus (SLE)

Comunicaciones a congreso

“Now I’m afraid to go out alone, because I’m afraid to losing my self”: performance and participation in daily life activities perception in a group of older persons with mild dementia

People with mild dementia may have many difficulties associated to clinical symptoms in daily life activities, but also the person perception about his daily performance might influence the participation in activities. To know the perception of the people with mild dementia about their performance in daily life activities, it was made a qualitative study, it was conducted through focus groups with 13 persons with dementia participants from Kintun Center. Groups of four to five persons were formed, where they discussed about two main topics: important activities and activities where they perceive difficulties. Through a hermeneutic-interpretative with open coding analysis, we observed two main themes in relation to daily activities performance: on one hand, the need of keep meaningful roles and on the other hand, the auto marginality and the dependence sensation imposed by the social environment. These findings highlight the importance of considering the perception of the person with dementia and their environment in relation to the possibilities of participation in daily activities, considering that not only the clinical symptoms may affect the participation, it may be affected also by the interpretation about daily situations

Efecto de algunas variables sobre la preñez de vaquillonas post-inseminación artificial a tiempo fijo

El objetivo del presente trabajo fue evaluar el efecto del tratamiento de control del ciclo estral (Co-Synch: 5 días; J-Synch o Dispositivos 7 días), del estado reproductivo (cíclico o anestro superficial) y de la gonadotrofina coriónica equina (eCG) sobre el porcentaje de preñez post-inseminación artificial a tiempo fijo (IATF). Se utilizaron vaquillonas Angus de 20-22 meses (n=336), que fueron distribuidas a: Co-Synch 5 días (el día 0 se colocó un dispositivo intravaginal DISP (0,558 g progesterona más 10,5 µg de acetato de buserelina). El día 5 se retiró el DISP y se inyectó 0,150 mg de D-Cloprostenol y aleatoriamente recibieron o no 300 UI de eCG. A las 56 h post DISP se realizó IATF más administración de 10,5 mg de acetato de buserelina. J-Synch: el día 0 se colocó DISP más 2 mg de benzoato de estradiol. El día 6 se retiró el DISP y se inyectó 0,150 mg de D-Cloprostenol; y aleatoriamente recibieron o no 300 UI de eCG. A las 72 h post DISP se realizó IATF más 10,5 mg de acetato de buserelina. Dispositivo 7 días: el día 0 se colocó DISP más 2 mg de benzoato de estradiol. El día 7 se retiró el DISP y se inyectaron 0,150 mg de D-Cloprostenol y 1 mg de cipionato de estradiol; aleatoriamente recibieron o no 300 UI de eCG. El diagnóstico de gestación se realizó a los 30 días post IATF. Se observaron efectos significativos (p<0,01) del tratamiento de control del ciclo estral sobre el porcentaje de preñez (Co-Synch 5 días: 38,1a , J-Synch: 55,5b y Dispositivo 7 días: 61,1b ), del estado reproductivo (cíclico: 58,7; anestro superficial: 42,2) y de la administración de eCG (Si: 59,2 y No: 43,7). Se concluye que el tratamiento hormonal, el estado reproductivo y la administración de eCG afectan el porcentaje de preñez post IATF&gt

Respuesta reproductiva de la oveja a la administración de gonadotrofina coriónica humana en el momento del servicio

Se evaluó la respuesta reproductiva a la administración de gonadotrofina coriónica humana (hCG), administrada al momento del servicio en ovejas mestizas (Frisona x Corriedale) con diferente condición corporal (CC) durante el servicio de otoño (Ensayo 1) y durante el servicio de primavera en ovejas que previamente recibieron un tratamiento de inducción de celos (Ensayo 2). Ensayo 1: se realizó detección de celos durante 16 días e inseminación artificial con semen fresco. Se evaluó CC de los animales en celo conformándose 2 grupos: ACC (CC mayor o igual a 2,5) y BCC (CC menor a 2,5). En cada grupo, las ovejas fueron distribuidas en 2 subgrupos (hCG y Control). Ensayo 2: se realizó tratamiento de inducción de celos con esponja intravaginal con progestágeno por 10 días y 400 UI de gonadotrofina coriónica equina (eCG) a su retiro. Durante los 3 días siguientes al tratamiento, las ovejas fueron alojadas con 5 carneros para realizar el servicio y distribuidas en dos grupos (hCG y Control). En ambos ensayos, en los grupos hCG se administraron 150 UI de hCG/animal y en los grupos Control, 1 ml de solución fisiológica/animal, vía intramuscular. Se realizó ecografía para determinar los porcentajes de preñez (%P) y de ovejas con gestaciones múltiples (%OGM). Los datos se analizaron con el paquete estadístico SAS estableciéndose un 95% de significación. Ensayo 1: %P y %OGM fueron: 73,6% y 43,3% para el grupo hCG y 64,7% y 36,4% para el grupo Control (p>0,05). Ensayo 2: %P y %OGM fueron: 55,2% y 31,2% para el grupo hCG y 36,0% y 22,2% para el grupo Control (p>0,05). Se concluye que la administración de hCG al momento del servicio no mejora la respuesta reproductiva de ovejas inseminadas artificialmente en temporada reproductiva u ovejas con servicio con carneros que recibieron previamente un tratamiento de inducción de celos

Discovery of circulating miRNAs as biomarkers of chronic Chagas heart disease via a small RNA-Seq approach

Circulating miRNAs; Biomarkers; Chronic Chagas heart diseasemiARN circulantes; Biomarcadores; Enfermedad cardíaca crónica de ChagasmiRNAs circulants; Biomarcadors; Malaltia cardíaca crònica de ChagasChagas disease affects approximately 7 million people worldwide in Latin America and is a neglected tropical disease. Twenty to thirty percent of chronically infected patients develop chronic Chagas cardiomyopathy decades after acute infection. Identifying biomarkers of Chagas disease progression is necessary to develop better therapeutic and preventive strategies. Circulating microRNAs are increasingly reliable biomarkers of disease and therapeutic targets. To identify new circulating microRNAs for Chagas disease, we performed exploratory small RNA sequencing from the plasma of patients and performed de novo miRNA prediction, identifying potential new microRNAs. The levels of the new microRNAs temporarily named miR-Contig-1519 and miR-Contig-3244 and microRNAs that are biomarkers for nonchagasic cardiomyopathies, such as miR-148a-3p and miR-224-5p, were validated by quantitative reverse transcription. We found a specific circulating microRNA signature defined by low miR-Contig-3244, miR-Contig-1519, and miR-148a-3 levels but high miR-224-5p levels for patients with chronic Chagas disease. Finally, we predicted in silico that these altered circulating microRNAs could affect the expression of target genes involved in different cellular pathways and biological processes, which we will explore in the future.The funding was supported by Agencia Nacional e Promoción Científica y Tecnológica, PICT 2013-1892, Secretaría de Ciencia y Tecnología, Universidad Nacional de Rosario, 1MED410, Ministerio de Economía y competitividad and Fondo Europeo de Desarrollo Regional, SAF2016-75988-R (MINECO/FEDER), SAF2015-63868-R (MINECO/FEDER), Red de Investigación de Centros de Enfermedades Tropicales, RICET RD12/0018/0004, Consejería de Sanidad, Comunidad de Madrid, S-2010/BMD-2332, Ministerio de Ciencia, Innovación y Universidades-Agencia Estatal de Investigación and Fondo Europeo de Desarrollo Regional, PGC2018-096132-B-I00

A novel phosphatidylinositol 3-kinase (PI3K) inhibitor directs a potent FOXO-dependent, p53-independent cell cycle arrest phenotype characterized by the differential induction of a subset of FOXO-regulated genes.

INTRODUCTION: The activation of the phosphoinositide 3-kinase (PI3K)/AKT signalling pathway is one the most frequent genetic events in breast cancer, consequently the development of PI3K inhibitors has attracted much attention. Here we evaluate the effect of PI3K inhibition on global gene expression in breast cancer cells. METHODS: We used a range of methodologies that include in silico compound analysis, in vitro kinase assays, cell invasion assays, proliferation assays, genome-wide transcription studies (Agilent Technologies full genome arrays), gene set enrichment analysis, quantitative real-time PCR, immunoblotting in addition to chromatin immunoprecipitation. RESULTS: We defined the physico-chemical and the biological properties of ETP-45658, a novel potent PI3K inhibitor. We demonstrated that ETP-45658 potently inhibited cell proliferation within a broad range of human cancer cells, most potently suppressing the growth of breast cancer cells via inhibiting cell cycle. We show that this response is Forkhead box O (FOXO) protein dependent and p53 independent. Our genome-wide microarray analysis revealed that the cell cycle was the most affected biological process after exposure to ETP-45658 (or our control PI3K inhibitor PI-103), that despite the multiple transcription factors that are regulated by the PI3K/AKT signalling cascade, only the binding sites for FOXO transcription factors were significantly enriched and only a subset of all FOXO-dependent genes were induced. This disparity in gene transcription was not due to differential FOXO promoter recruitment. CONCLUSIONS: The constitutive activation of PI3Ks and thus the exclusion of FOXO transcription factors from the nucleus is a key feature of breast cancer. Our results presented here highlight that PI3K inhibition activates specific FOXO-dependent genes that mediate cell cycle arrest in breast cancer cells