Using 3D photogrammetry from ROV video to quantify cold-water coral reef structural complexity and investigate its influence on biodiversity and community assemblage

Fine-scale structural complexity created by reef-building coral in shallow-water environments is influential on biodiversity, species assemblage and functional trait expression. Cold-water coral reefs are also hotspots of biodiversity, often attributed to the hard surface and structural complexity provided by the coral. However, that complexity has seldom been quantified on a centimetric scale in cold-water coral reefs, unlike their shallow-water counterparts, and has therefore never been linked in a similar way to the reef inhabitant community. Structure from motion techniques which create high-resolution 3D models of habitats from sequences of photographs is being increasingly utilised, in tandem with 3D spatial analysis to create useful 3D metrics, such as rugosity. Here, we demonstrate the use of ROV video transect data for 3D reconstructions of cold-water coral reefs at depths of nearly 1000 m in the Explorer Canyon, a tributary of Whittard Canyon, NE Atlantic. We constructed 40 3D models of approximately 25-m-length video transects using Agisoft Photoscan software, resulting in sub-centimetre resolution reconstructions. Digital elevation models were utilised to derive rugosity metrics, and orthomosaics were used for coral coverage assessment. We found rugosity values comparable to shallow-water tropical coral reef rugosity. Reef and nearby non-reef communities differed in assemblage composition, which was driven by depth and rugosity. Species richness, epifauna abundance and fish abundance increased with structural complexity, being attributed to an increase in niches, food, shelter and alteration of physical water movement. Biodiversity plateaued at higher rugosity, illustrating the establishment of a specific reef community supported by more than 30% coral cover. The proportion of dead coral to live coral had limited influence on the community structure; instead, within-reef patterns were explained by depth and rugosity, though our results were confounded to a certain extent by multi-collinearity. Fine-scale structural complexity appeared to be integral to local-scale ecological patterns in cold-water coral reef communities

Museum Magazine, Number 68 (2016 Winter)

The issue's feature article, "Afro-Cuban artists: a Renaissance. Manuel Mendive and Eduardo "Choco" Roca Salazar February 23-May 1, 2016," focuses on two of the most celebrated Cuban artists working today. Both men benefited from the educational and cultural initiatives instituted by Fidel Castro following the 1959 Cuban Revolution and receive inspiration from their Afro-Cuban heritage. However, their distinct selections of subject matter and divergent styles underscore the manifold ways revolution and race continue to be interpreted and understood on the island today.From the Director / Alex W. Barker (Director) -- Afro-Cuban artists : a renaissance / Kristin Schwain (Associate Professor, Art History) -- Black American artists : envisioning social change / Alisa Carlson (Curator of European and American Art) -- Recent acquisition : a German Renaissance portrait / Alisa Carlson (Curator of European and American Art) -- Special exhibitions -- Events calendar -- Missouri Folk Arts Program / Lisa Overholser (Guest Author) -- From the Museum Educator / Cathy Callaway (Museum Educator) -- From the Academic Coordinator / Arthur Mehrhoff -- Cleaning an old master / Alex Barker (Director) -- Spotlight : The Moon God Men / Benton Kidd (Curator of Ancient Art) -- Museum Associates / Gary Anger (President, Museum Associates)

Maternal super-obesity and perinatal outcomes in Australia: A national population-based cohort study

© 2015 Sullivan et al. Background: Super-obesity is associated with significantly elevated rates of obstetric complications, adverse perinatal outcomes and interventions. The purpose of this study was to determine the prevalence, risk factors, management and perinatal outcomes of super-obese women giving birth in Australia. Methods: A national population-based cohort study. Super-obese pregnant women (body mass index (BMI) >50 kg/m2 or weight >140 kg) who gave birth between January 1 and October 31, 2010 and a comparison cohort were identified using the Australasian Maternity Outcomes Surveillance System (AMOSS). Outcomes included maternal and perinatal morbidity and mortality. Prevalence estimates calculated with 95 % confidence intervals (CIs). Adjusted odds ratios (ORs) were calculated using multivariable logistic regression. Results: 370 super-obese women with a median BMI of 52.8 kg/m2 (range 40.9-79.9 kg/m2) and prevalence of 2.1 per 1 000 women giving birth (95 % CI: 1.96-2.40). Super-obese women were significantly more likely to be public patients (96.2 %), smoke (23.8 %) and be socio-economically disadvantaged (36.2 %). Compared with other women, super-obese women had a significantly higher risk for obstetric (adjusted odds ratio (AOR) 2.42, 95 % CI: 1.77-3.29) and medical (AOR: 2.89, 95 % CI: 2.64-4.11) complications during pregnancy, birth by caesarean section (51.6 %) and admission to special care (HDU/ICU) (6.2 %). The 372 babies born to 365 super-obese women with outcomes known had significantly higher rates of birthweight ≥4500 g (AOR 19.94, 95 % CI: 6.81-58.36), hospital transfer (AOR 3.81, 95 % CI: 1.93-7.55) and admission to Neonatal Intensive Care Unit (NICU) (AOR 1.83, 95 % CI: 1.27-2.65) compared to babies of the comparison group, but not prematurity (10.5 % versus 9.2 %) or perinatal mortality (11.0 (95 % CI: 4.3-28.0) versus 6.6 (95 % CI: 2.6- 16.8) per 1 000 singleton births). Conclusions: Super-obesity in pregnancy in Australia is associated with increased rates of pregnancy and birth complications, and with social disadvantage. There is an urgent need to further address risk factors leading to super-obesity among pregnant women and for maternity services to better address pre-pregnancy and pregnancy care to reduce associated inequalities in perinatal outcomes

Neocortical Axon Arbors Trade-off Material and Conduction Delay Conservation

The brain contains a complex network of axons rapidly communicating information between billions of synaptically connected neurons. The morphology of individual axons, therefore, defines the course of information flow within the brain. More than a century ago, Ramón y Cajal proposed that conservation laws to save material (wire) length and limit conduction delay regulate the design of individual axon arbors in cerebral cortex. Yet the spatial and temporal communication costs of single neocortical axons remain undefined. Here, using reconstructions of in vivo labelled excitatory spiny cell and inhibitory basket cell intracortical axons combined with a variety of graph optimization algorithms, we empirically investigated Cajal's conservation laws in cerebral cortex for whole three-dimensional (3D) axon arbors, to our knowledge the first study of its kind. We found intracortical axons were significantly longer than optimal. The temporal cost of cortical axons was also suboptimal though far superior to wire-minimized arbors. We discovered that cortical axon branching appears to promote a low temporal dispersion of axonal latencies and a tight relationship between cortical distance and axonal latency. In addition, inhibitory basket cell axonal latencies may occur within a much narrower temporal window than excitatory spiny cell axons, which may help boost signal detection. Thus, to optimize neuronal network communication we find that a modest excess of axonal wire is traded-off to enhance arbor temporal economy and precision. Our results offer insight into the principles of brain organization and communication in and development of grey matter, where temporal precision is a crucial prerequisite for coincidence detection, synchronization and rapid network oscillations

Two-stage Genome-wide Methylation Profiling in Childhood-onset Crohn's Disease Implicates Epigenetic Alterations at the VMP1/MIR21 and HLA Loci

Background: As a result of technological and analytical advances, genome-wide characterization of key epigenetic alterations is now feasible in complex diseases. We hypothesized that this may provide important insights into gene-environmental interactions in Crohn's disease (CD) and is especially pertinent to early onset disease. Methods: The Illumina 450K platform was applied to assess epigenome-wide methylation profiles in circulating leukocyte DNA in discovery and replication pediatric CD cohorts and controls. Data were corrected for differential leukocyte proportions. Targeted replication was performed in adults using pyrosequencing. Methylation changes were correlated with gene expression in blood and intestinal mucosa. Results: We identified 65 individual CpG sites with methylation alterations achieving epigenome-wide significance after Bonferroni correction (P &#60; 1.1 × 10−7), and 19 differently methylated regions displaying unidirectional methylation change. There was a highly significant enrichment of methylation changes around GWAS single nucleotide polymorphisms (P = 3.7 × 10−7), notably the HLA region and MIR21. Two-locus discriminant analysis in the discovery cohort predicted disease in the pediatric replication cohort with high accuracy (area under the curve, 0.98). The findings strongly implicate the transcriptional start site of MIR21 as a region of extended epigenetic alteration, containing the most significant individual probes (P = 1.97 × 10−15) within a GWAS risk locus. In extension studies, we confirmed hypomethylation of MIR21 in adults (P = 6.6 × 10−5, n = 172) and show increased mRNA expression in leukocytes (P &#60; 0.005, n = 66) and in the inflamed intestine (P = 1.4 × 10−6, n = 99). Conclusions: We demonstrate highly significant and replicable differences in DNA methylation in CD, defining the disease-associated epigenome. The data strongly implicate known GWAS loci, with compelling evidence implicating MIR21 and the HLA region.</p

The contribution of X-linked coding variation to severe developmental disorders

Abstract: Over 130 X-linked genes have been robustly associated with developmental disorders, and X-linked causes have been hypothesised to underlie the higher developmental disorder rates in males. Here, we evaluate the burden of X-linked coding variation in 11,044 developmental disorder patients, and find a similar rate of X-linked causes in males and females (6.0% and 6.9%, respectively), indicating that such variants do not account for the 1.4-fold male bias. We develop an improved strategy to detect X-linked developmental disorders and identify 23 significant genes, all of which were previously known, consistent with our inference that the vast majority of the X-linked burden is in known developmental disorder-associated genes. Importantly, we estimate that, in male probands, only 13% of inherited rare missense variants in known developmental disorder-associated genes are likely to be pathogenic. Our results demonstrate that statistical analysis of large datasets can refine our understanding of modes of inheritance for individual X-linked disorders

Jejunal diverticulitis: an unusual cause of an intra-abdominal abscess - coronal Computed Tomography reconstruction can aid the diagnosis

Jejunal diverticulitis is a rare condition that can present with an acute abdomen and be referred for imaging. We present the case of an elderly patient who at CT was diagnosed with an intra-abdominal abscess involving both jejunum and transverse colon. However, the underlying eitiology was not initially clear until small bowel barium follow-through