Can Wheat Germ Have a Beneficial Effect on Human Health? A Study Protocol for a Randomised Crossover Controlled Trial to Evaluate its Health Effects

INTRODUCTION: Cardiovascular diseases (CVD) are the leading cause of mortality worldwide and diet is an important contributor to CVD risk. Thus, several food derivatives are being investigated for their beneficial impact on reducing cardiometabolic risk factors, either in risk groups or in healthy population as a preventive measure. Wheat germ is a food by-product with high nutritional value, especially as a concentrated source of dietary fibre and essential fatty acids, but its incorporation into the diet has been rare up to now. Previous studies do not clarify the hypothesised potential causal relationship between the consumption of wheat germ and benefits for human health. METHODS AND ANALYSIS: We are conducting a randomised, double-blinded, crossover, placebo-controlled clinical trial designed to assess the physiological effects of daily consumption of wheat germ-enriched bread (containing 6 g of wheat germ) compared with non-enriched bread, over a 4-week period with a 15-week follow-up, in a healthy human population. A total of 55 participants (healthy volunteers, aged 18-60) have been recruited from the Porto metropolitan area in northern Portugal. Our aim is to evaluate the health effects of wheat germ on blood cholesterol and triglycerides, postprandial glycaemic response, gastrointestinal function and discomfort, and changes in intestinal microbiota and insulin resistance as secondary outcomes. The study follows the best practices for evaluating health claims in food according to the European Food Safety Authority (EFSA) scientific opinion, namely random allocation, double blinding, reporting methods to measure and maximise compliance, and validated outcomes with beneficial physiological effects as recommended by EFSA. ETHICS AND DISSEMINATION: The study has been approved by the Health Ethics Committee of São João Hospital Centre (156-15) and the Ethics Committee of Faculty of Medicine of the University of Porto (PCEDCSS-FMUP07/2015). Results will be disseminated through peer-reviewed publications and presentations at international scientific meetings.info:eu-repo/semantics/publishedVersio

Attachment, Physiological and Familial Vulnerability in Childhood Obesity: an Interactive Multisystem Approach

The aims of the present study were to test the association between insecure attachment and basal cortisol and catecholamines levels in a sample of obese children. The role of familial vulnerability and gender was also investigated. Methods: Cortisol and catecholamines levels of 8- to 13-year olds obese children were measured. Self-report questionnaires were used to assess attachment pattern and current anxiety and depression, and parent-report questionnaires were used to assess attachment, current anxiety and depression and familial vulnerability. Linear regression analyses were performed for individuals that scored low versus high on parental internalizing problems, and for boys and girls, separately. Results: In the group with high parental internalizing problems, insecure attachment was significantly associated with reduced basal levels of cortisol, in boys (p=0.007, b= -0.861, R2= 73.0%). In the group with low parental internalizing problems, the association between insecure attachment and cortisol was not significant in either boys or girls, and it was negative in boys (p=0.075, b= -0.606, R2= 36.7%) and positive in girls (p=0.677, b= 0.176, R2= 3.1%) . Conclusions: Apparently, physiological risk factors for psicopathology in obesity are more evident in individuals with a high familial vulnerability. In addition, patterns of physiological risk for psicopathology in obesity are different in boys and girls. Therefore, it is important to take into account familial vulnerability and gender when investigating physiological risk factors for psycopathology in obesity. Insecure attachment in childhood may be a risk factor for obesity. Interventions to increase children's attachment security should examine the effects on children's weight

High-fat diet-induced obesity rat model: a comparison between wistar and sprague-dawley rat

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Daily Intake of Wheat Germ-Enriched Bread May Promote a Healthy Gut Bacterial Microbiota: a Randomised Controlled Trial

Purpose: Wheat bran fibre has a beneficial effect on gastrointestinal function, but evidence for wheat germ is scarce. Accordingly, we evaluated the effects of daily intake of wheat germ on gastrointestinal discomfort and gut microbiota by adding wheat germ to refined (white) wheat bread, the most consumed bread type. We hypothesised that an improvement in the composition of refined bread could beneficially affect intestinal health without compromising consumers' acceptance. Methods: Fifty-five healthy adults were recruited for a randomised, double-blind, crossover, controlled trial comprising two 4-week intervention periods separated by a 5-week washout stage. During the first 4-week period, one group consumed wheat bread enriched with 6 g of wheat germ and the control group consumed non-enriched wheat bread. Results: Wheat germ-enriched bread was well-appreciated and the number of participants that demonstrated minimal gastrointestinal improvements after wheat-germ intake was higher than in the control arm. Importantly, intake of wheat germ-enriched bread decreased the perceived gastrointestinal discomfort-related quality of life (subscale worries and concerns) over refined white bread. The improvements in the gastrointestinal function were accompanied by favourable changes in gut microbiota, increasing the number of Bacteroides spp. and Bifidobacterium spp. Conclusions: Adding wheat germ to industrially made white bread without altering sensory properties may promote a healthy gut bacterial microbiota and the gastrointestinal health.info:eu-repo/semantics/publishedVersio

FEEDMI: A Study Protocol to Determine the Influence of Infant-Feeding on Very-Preterm-Infant's Gut Microbiota

Background: Preterm infants are especially vulnerable to gut microbiota disruption and dysbiosis since their early gut microbiota is less abundant and diverse. Several factors may influence infants' gut microbiota, such as the mother's diet, mode of delivery, antibiotic exposure, and type of feeding. Objectives: This study aims to examine the factors associated with very-preterm neonate's intestinal microbiota, namely: (1) type of infant-feeding (breast milk, donor human milk with or without bovine protein-based fortifier, and preterm formula); (2) maternal diet; and (3) mode of delivery. Methods: This is an observational study conducted in a cohort of very preterm infants hospitalized in the neonatal intensive care unit of Maternidade Dr. Alfredo da Costa. After delivery, the mothers are asked to collect their own fecal samples and are invited to complete a semiquantitative food frequency questionnaire. The maternal diet will be classified in accordance to the Mediterranean Diet adherence score. Stool samples have been collected from very premature infants every 7 days for 21 days. DNA has been extracted from the fecal samples, and different bacterial genus and species will be quantified by real-time polymerase chain reaction. Results and conclusions: It is hypothesized that significant differences in the microbiota composition and clinical outcomes of very preterm infants will be observed depending on the type of infant feeding. In addition, this study will clarify how pasteurized donor's milk influences the intestinal microbiota colonization of preterm infants. This is a pioneer study developed in collaboration with the country's Human Milk Bank. We also expect to find microbiota alterations in infants according to the mode of delivery and to maternal diet. This study will contribute to increase the evidence on the effects of breast or donor human milk and its fortification with a bovine protein-based fortifier on infant microbiota.info:eu-repo/semantics/publishedVersio

The growth of typical star-forming galaxies and their supermassive black holes across cosmic time since z~2

Understanding galaxy formation and evolution requires studying the interplay between the growth of galaxies and the growth of their black holes across cosmic time. Here, we explore a sample of Hα-selected star-forming galaxies from the High Redshift Emission Line Survey and use the wealth of multiwavelength data in the Cosmic Evolution Survey field (X-rays, far-infrared and radio) to study the relative growth rates between typical galaxies and their central supermassive black holes, from z = 2.23 to z = 0. Typical star-forming galaxies at z ∼ 1–2 have black hole accretion rates (M˙BHM˙BH) of 0.001–0.01 M⊙ yr−1 and star formation rates (SFRs) of ∼10–40 M⊙ yr−1, and thus grow their stellar mass much quicker than their black hole mass (3.3±0.2 orders of magnitude faster). However, ∼3 per cent of the sample (the sources detected directly in the X-rays) show a significantly quicker growth of the black hole mass (up to 1.5 orders of magnitude quicker growth than the typical sources). M˙BHM˙BH falls from z = 2.23 to z = 0, with the decline resembling that of SFR density or the typical SFR (SFR*). We find that the average black hole to galaxy growth (M˙BHM˙BH/SFR) is approximately constant for star-forming galaxies in the last 11 Gyr. The relatively constant M˙BHM˙BH/SFR suggests that these two quantities evolve equivalently through cosmic time and with practically no delay between the two

Salt reduction in different bread types: an intervention study in the Northern Portugal

O pão é um dos alimentos mais antigos e consumidos em todo o Mundo, sendo considerado uma boa fonte de diversos nutrientes fundamentais para o desenvolvimento e saúde do organismo. Os dados do Inquérito Alimentar Nacional e de Atividade Física IAN-AF (2015/2016), em Portugal, identificam o pão como sendo considerado um dos principais alimentos a contribuir para a ingestão de sal dos portugueses. A Sociedade Portuguesa de Hipertensão, em colaboração com a Associação dos Industriais de Panificação, Pastelaria e Similares do Norte e o Instituto Nacional de Saúde Doutor Ricardo Jorge, desenvolveram um estudo interventivo com vista a reduzir o teor de sal no pão sem alterar as suas propriedades organoléticas. O objetivo deste estudo foi avaliar o teor de sal em 4 tipos de pão tradicional: carcaça, regueifa, broa e pão da avó, antes e depois da intervenção planeada. Antes da intervenção verificou-se que os valores de teor de sal encontrados, em todos os tipos de pão analisados, estavam abaixo de 1,4 g de sal por 100 g de pão (valor estabelecido pelo Decreto- -Lei n.º 75/2009, de 12 de agosto) e que, após intervenção, foi possível reduzir para valores entre 0,96 e 1,1 g de sal por 100 g de pão, sem alterar as características organoléticas. O estudo realizado demonstrou ser possível a redução do teor de sal no pão, sem alterações adicionais no processo de produção, constituindo uma medida de baixo custo que permite a melhoria da saúde pública, relativamente à prevenção de doenças crónicas associadas ao consumo excessivo de sal.Bread is a key staple in the Portuguese diet and provides many of the nutrients required for normal development and good health. The National Food and Physical Activity Survey IAN-AF (2015/2016), in Portugal, identify bread as one of the main foods contributing to Portuguese salt intake. The Portuguese Society of Hypertension, in collaboration with the Portuguese Bakeries Association from North and the National Institute of Health Dr. Ricardo Jorge, planned an intervention study to reduce the content of salt in the bread, without changing its organoleptic properties. The objective of this study was to evaluate the salt content in four types of traditional bread: “carcaça”, “regueifa”, “broa” e “pão da avó”, before and after the planned intervention. It was found that the values of salt content before the intervention were below the value established in legislation nº 75/2009 of 1.4 g of salt per 100 g of bread and that, after intervention it was possible to reduce the salt content to values between 0.96 and 1.1 g of salt per 100 g of bread, without changing the organoleptic characteristics. The study showed that it is possible to reduce the salt content in bread without additional changes in the production process, and it is a low-cost measure allowing the improvement of public health in relation to the prevention of chronic diseases associated with excessive salt consumptioninfo:eu-repo/semantics/publishedVersio

Discovery of faint double-peak Halpha emission in the halo of low redshift galaxies

Aiming at the detection of cosmological gas being accreted onto galaxies of the local Universe, we examined the Halpha emission in the halo of 164 galaxies in the field of view of the Multi-Unit Spectroscopic Explorer Wide survey (\musew ) with observable Halpha (redshift < 0.42). An exhaustive screening of the corresponding Halpha images led us to select 118 reliable Halpha emitting gas clouds. The signals are faint, with a surface brightness of 10**(-17.3 pm 0.3) erg/s/cm2/arcsec2. Through statistical tests and other arguments, we ruled out that they are created by instrumental artifacts, telluric line residuals, or high redshift interlopers. Around 38% of the time, the Halpha line profile shows a double peak with the drop in intensity at the rest-frame of the central galaxy, and with a typical peak-to-peak separation of the order of pm 200 km/s. Most line emission clumps are spatially unresolved. The mass of emitting gas is estimated to be between one and 10**(-3) times the stellar mass of the central galaxy. The signals are not isotropically distributed; their azimuth tends to be aligned with the major axis of the corresponding galaxy. The distances to the central galaxies are not random either. The counts drop at a distance > 50 galaxy radii, which roughly corresponds to the virial radius of the central galaxy. We explore several physical scenarios to explain this Halpha emission, among which accretion disks around rogue intermediate mass black holes fit the observations best.Comment: pay attention to the last sentence of the abstract! Accepted for publication in Ap

POPs’ effect on cardiometabolic and inflammatory profile in a sample of women with obesity and hypertension

Persistent organic pollutants (POPs) are man-made compounds with metabolic disruption impact. We investigated the effect of POP exposure in the cardiometabolic and inflammatory profile in a population of women with obesity and hypertension. In 43 premenopausal women (22 treated vs. 21 nontreated) undergoing bariatric surgery, blood and adipose tissue samples (visceral (vAT) and abdominal subcutaneous (scAT)) were collected. Median concentrations of ∑HCH and ∑POPs in vAT were significantly higher in treated women. VAT ∑HCH and scAT ∑HCH and ∑POPs concentrations were positively correlated with systolic blood pressure in the non-treated group. Our findings suggest that exposure to POPs and its accumulation in vAT and circulating in plasma may be associated to a higher cardiovascular risk in women with obesity and hypertension, with or without antihypertensive treatment.This work was supported by FCT (Fundo Social Europeu, Programa Operacional Potencial Humano da EU (POPH); PEst-OE/SAU/UI0038/2011; SFRH/BD/46640/2008, SFRH/BD/64691/2009), and Projetos de Investigação na Pré-graduação 2011, Universidade do Porto.info:eu-repo/semantics/publishedVersio

One-pot intercalation strategy for the encapsulation of a CO-releasing organometallic molecule in a layered double hydroxide

The photoactivatable CO‐releasing molecule (photoCORM) [Mo(CO)3(CNCH2COOH)3] (ALF795) has been incorporated into a Zn,Al layered double hydroxide (LDH) host by a coprecipitation synthesis strategy. Powder X‐ray diffraction (PXRD) of the resultant material Zn,Al‐ALF795 showed that the ALF795 guest molecules assembled into a monolayer to give a basal spacing of 16.0 Å. FTIR and 13C{1H} CP MAS NMR spectroscopy confirmed that the molecular structure of the tricarbonyl complex was retained upon intercalation. Scanning electron microscopy (SEM), energy‐dispersive X‐ray spectroscopy (EDS) and elemental analyses confirmed the phase‐purity of Zn,Al‐ALF795. The myoglobin assay was used to demonstrate that intercalated ALF795 retains the photoactive behavior of the free CORM, with a substantial fraction (42 %) of the high CO payload (2.46 mmol g–1) being released after exposure to UV light for 3 h under simulated physiological conditions. In addition, gas chromatography was used to track sequential light‐ and H2O2‐triggered decarbonylation of free and intercalated ALF795. In biological buffer solution (HEPES), less than 2 % Mo leaching from Zn,Al‐ALF795 took place after 5 h, showing the strong capacity of the LDH host to retain the unaltered complex and decarbonylation fragments.publishe