Monitoring coastal dolphins within the Reef 2050 Integrated Monitoring and Reporting Program: final report of the dolphins team in the megafauna expert group

Three species of coastal dolphins are commonly found throughout the Great Barrier Reef (the Reef), the Australian snubfin dolphin, Orcaella heinsohni, the Australian humpback dolphin, Sousa sahulensis, and the Indo-Pacific bottlenose dolphin, Tursiops aduncus. This report focuses on these three species, acknowledging that many other cetacean species also inhabit the Reef. This report is a desktop study that includes current status of the relevant elements of the Reef, priority indicators for monitoring, potential sources of data, assessment of the adequacy of existing monitoring activities and recommendations for the design of an integrated monitoring program as a component of RIMReP

CLIMATOLOGICAL STUDY OF THE WIND IN PIEDMONT

The object of the following study is to individualize the common anemological features in the Piemonte region. The executed analysis avails itself of a multiannual sample of wind data (from 1990 to 2000) collected by 59 ARPA-Piemonte monitoring network. The wind statistical analyses have followed different phases: - analyses of wind frequency and wind directions and spotting of the anemological basins; - annual and monthly average of wind directions; - annual monthly and hourly average of the wind force with spotting of breezes; - analyses of daily and hourly wind calm; - analyses of the strongest windblasts with records of critical cases; - analyses of the number of days of föhn and types of weather (Borghi-Giuliacci method)

CLIMATOLOGICAL STUDY OF THE WIND IN PIEDMONT

The object of the following study is to individualize the common anemological features in the Piemonte region. The executed analysis avails itself of a multiannual sample of wind data (from 1990 to 2000) collected by 59 ARPA-Piemonte monitoring network. The wind statistical analyses have followed different phases: - analyses of wind frequency and wind directions and spotting of the anemological basins; - annual and monthly average of wind directions; - annual monthly and hourly average of the wind force with spotting of breezes; - analyses of daily and hourly wind calm; - analyses of the strongest windblasts with records of critical cases; - analyses of the number of days of föhn and types of weather (Borghi-Giuliacci method)

Isotopic niche overlap between sympatric Australian snubfin and humpback dolphins

Ecological niche theory predicts the coexistence of closely related species is promoted by resource partitioning in space and time. Australian snubfin (Orcaella heinsohni) and humpback (Sousa sahulensis) dolphins live in sympatry throughout most of their range in northern Australian waters. We compared stable isotope ratios of carbon (δ13C) and nitrogen (δ15N) in their skin to investigate resource partitioning between these ecologically similar species. Skin samples were collected from live Australian snubfin (n = 31) and humpback dolphins (n = 23) along the east coast of Queensland in 2014–2015. Both species had similar δ13C and δ15N values and high (>50%) isotopic niche space overlap, suggesting that they feed at similar trophic levels, have substantial dietary overlap, and rely on similar basal food resources. Despite similarities, snubfin dolphins were more likely to have a larger δ15N value than humpback dolphins, indicating they may forage on a wider diversity of prey. Humpback dolphins were more likely to have a larger δ13C range suggesting they may forage on a wider range of habitats. Overall, results suggest that subtle differences in habitat use and prey selection are likely the principal resource partitioning mechanisms enabling the coexistence of Australian snubfin and humpback dolphins

SONiCS: PCR stutter noise correction in genome-scale microsatellites

Motivation Massively parallel capture of short tandem repeats (STRs, or microsatellites) provides a strategy for population genomic and demographic analyses at high resolution with or without a reference genome. However, the high Polymerase Chain Reaction (PCR) cycle numbers needed for target capture experiments create genotyping noise through polymerase slippage known as PCR stutter. Results We developed SONiCS—Stutter mONte Carlo Simulation—a solution for stutter correction based on dense forward simulations of PCR and capture experimental conditions. To test SONiCS, we genotyped a 2499-marker STR panel in 22 humpback dolphins (Sousa sahulensis) using target capture, and generated capillary-based genotypes to validate five of these markers. In these 110 comparisons, SONiCS showed a 99.1% accuracy rate and a 98.2% genotyping success rate, miscalling a single allele in a marker with low sequence coverage and rejecting another as un-callable. Availability and implementation Source code and documentation for SONiCS is freely available at https://github.com/kzkedzierska/sonics. Raw read data used in experimental validation of SONiCS have been deposited in the Sequence Read Archive under accession number SRP135756

Best practice framework and principles for monitoring the effect of coastal development on marine mammals

As the worlds human population increases along the coastal zone with major alteration of coastal embayments increased on-water activities and a plethora of other intrusions into the coastal zone there is a simultaneous increase in pressures on marine mammals. Growing evidence indicates that many marine mammals are highly susceptible to declines resulting from direct and indirect impacts arising from diverse human activities. Too frequently assessment of the impact from coastal developments on marine mammals has been inadequate or completely lacking. At worst this has led to catastrophic decline in some populations. Without rigorous ecological assessments along with adaptive management frameworks prior to the initiation of developments the number of marine mammal populations likely to be adversely impacted will continue to rise. To address these shortcomings we present a globally applicable best practice framework by; (i) describing guiding principles and; (ii) reviewing appropriate procedures for assessment and monitoring of impacts of coastal developments on marine mammals. The approach outlined is embedded in Environmental Impact Assessment processes as a means by which decision makers and stakeholders can be informed. Recommendations presented are designed to encourage the application of robust scientific evaluation that applies appropriate survey design with sufficient statistical power to detect changes before trigger thresholds are reached. We emphasize that there is an urgent need to ensure assessments are comprehensive effective and integrated with monitoring and adaptive management actions in order to minimize or effectively mitigate the impacts of human activities on marine mammal population

Effects of pre-operative isolation on postoperative pulmonary complications after elective surgery: an international prospective cohort study

We aimed to determine the impact of pre-operative isolation on postoperative pulmonary complications after elective surgery during the global SARS-CoV-2 pandemic. We performed an international prospective cohort study including patients undergoing elective surgery in October 2020. Isolation was defined as the period before surgery during which patients did not leave their house or receive visitors from outside their household. The primary outcome was postoperative pulmonary complications, adjusted in multivariable models for measured confounders. Pre-defined sub-group analyses were performed for the primary outcome. A total of 96,454 patients from 114 countries were included and overall, 26,948 (27.9%) patients isolated before surgery. Postoperative pulmonary complications were recorded in 1947 (2.0%) patients of which 227 (11.7%) were associated with SARS-CoV-2 infection. Patients who isolated pre-operatively were older, had more respiratory comorbidities and were more commonly from areas of high SARS-CoV-2 incidence and high-income countries. Although the overall rates of postoperative pulmonary complications were similar in those that isolated and those that did not (2.1% vs 2.0%, respectively), isolation was associated with higher rates of postoperative pulmonary complications after adjustment (adjusted OR 1.20, 95%CI 1.05-1.36, p = 0.005). Sensitivity analyses revealed no further differences when patients were categorised by: pre-operative testing; use of COVID-19-free pathways; or community SARS-CoV-2 prevalence. The rate of postoperative pulmonary complications increased with periods of isolation longer than 3 days, with an OR (95%CI) at 4-7 days or >= 8 days of 1.25 (1.04-1.48), p = 0.015 and 1.31 (1.11-1.55), p = 0.001, respectively. Isolation before elective surgery might be associated with a small but clinically important increased risk of postoperative pulmonary complications. Longer periods of isolation showed no reduction in the risk of postoperative pulmonary complications. These findings have significant implications for global provision of elective surgical care