Grapholita molesta : characterization of an artificial rearing

Grapholita molesta (Busck) es considerada plaga principal del duraznero en Mendoza. Sus larvas han sido criadas, en condiciones de laboratorio, con dietas naturales por diversos autores. Los objetivos fueron: 1. poner a punto la cría artificial de la especie; 2. diseñar una jaula para el apareamiento y la oviposición de los adultos; 3. evaluar la eficiencia de la jaula y la dieta larvaria mediante grados día, ciclo biológico, peso de pupas, recuperación de huevoadulto, fecundidad, viabilidad y longevidad. La cría artificial de una especie constituye una herramienta para profundizar sus conocimientos bioetoecológicos y, en consecuencia, aplicarlos en su control. En 1996 se fundó una cría con larvas salvajes alimentadas con manzanas verdes pequeñas del cv. Granny Smith. Los adultos se desarrollaron en una jaula especialmente diseñada. En el ciclo biológico, la recuperación huevo-adulto y la fecundidad se obtuvieron valores superiores a los citados por otros autores pero no ocurrió lo mismo con las otras variables. La cría artificial de Grapholita molesta (Busck) se logró desarrollar por 37 generaciones y la alta fecundidad obtenida es una clave fundamental en el éxito de su mantenimiento.The Grapholita molesta (Busck) is considered the principal plague of peach trees in Mendoza. Its larva have been bred in laboratory conditions, with natural diets by different authors. The goals were: 1. to set the artificial breeding for this specie; 2. to design a cage for the mating and adult oviposition; 3. to evaluate the efficiency of the cage and larval diet by means of day egrees, biological cycle, pupae weight, egg-adult recovery, fertility, viability and longevity. The artificial rearing of a species constitutes a fundamental tool to obtain bioetoecological knowledge and in consequence apply it in its control. In 1996 offspring of wild larva which were fed small green Granny Smith apples were founded. The adults developed in a specially designed cage. In the biological cycle, egg-adult recovery and fertility superior values than those mentioned by other authors were obtained, but this didn't reoccur with the other variables. The artificial rearing of the Grapholita molesta (Busck) were able to develop for 37 generations, being the high fertility obtained a fundamental key in the success of their maintenance.Fil: Marín, María Susana. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Departamento de Ciencias BiológicasFil: Sáez, César C.. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Departamento de Ciencias BiológicasFil: Caballero, Ariel E.. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Departamento de Ciencias BiológicasFil: Quercetti, María J.. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Departamento de Ciencias Biológica

Plan de riesgos para la implementación, desarrollo y mantenimiento de componentes de web 2.0 en bibliotecas, caso de estudio en una biblioteca especializada

La era tecnológica y la globalización de la economía, ha traído como consecuencia el aumento sustancial de los riesgos en general, y en particular en el proceso software. En estos últimos tiempos la evolución de las aplicaciones tradicionales hacia aplicaciones Web enfocadas al usuario final que se denomina Web 2.0 y la no administración de los mismos puede implicar en cualquier Biblioteca la posibilidad del fracaso del mejor proyecto. La metodología desarrollada en este trabajo es una herramienta que brinda la posibilidad de efectuar tareas de identificación de riesgos, plan de aversión en base a taxonomías estándar para la implementación de componentes Web 2.0 en bibliotecas especializadas.Facultad de Humanidades y Ciencias de la Educació

Plan de riesgos para la implementación, desarrollo y mantenimiento de componentes de web 2.0 en bibliotecas, caso de estudio en una biblioteca especializada

La era tecnológica y la globalización de la economía, ha traído como consecuencia el aumento sustancial de los riesgos en general, y en particular en el proceso software. En estos últimos tiempos la evolución de las aplicaciones tradicionales hacia aplicaciones Web enfocadas al usuario final que se denomina Web 2.0 y la no administración de los mismos puede implicar en cualquier Biblioteca la posibilidad del fracaso del mejor proyecto. La metodología desarrollada en este trabajo es una herramienta que brinda la posibilidad de efectuar tareas de identificación de riesgos, plan de aversión en base a taxonomías estándar para la implementación de componentes Web 2.0 en bibliotecas especializadas.Facultad de Humanidades y Ciencias de la Educació

Plan de riesgos para la implementación, desarrollo y mantenimiento de componentes de web 2.0 en bibliotecas, caso de estudio en una biblioteca especializada

La era tecnológica y la globalización de la economía, ha traído como consecuencia el aumento sustancial de los riesgos en general, y en particular en el proceso software. En estos últimos tiempos la evolución de las aplicaciones tradicionales hacia aplicaciones Web enfocadas al usuario final que se denomina Web 2.0 y la no administración de los mismos puede implicar en cualquier Biblioteca la posibilidad del fracaso del mejor proyecto. La metodología desarrollada en este trabajo es una herramienta que brinda la posibilidad de efectuar tareas de identificación de riesgos, plan de aversión en base a taxonomías estándar para la implementación de componentes Web 2.0 en bibliotecas especializadas.Facultad de Humanidades y Ciencias de la Educació

Mutations in the EXT1 and EXT2 genes in Spanish patients with multiple osteochondromas

Multiple osteochondromas is an autosomal dominant skeletal disorder characterized by the formation of multiple cartilage-capped tumours. Two causal genes have been identified, EXT1 and EXT2, which account for 65% and 30% of cases, respectively. We have undertaken a mutation analysis of the EXT1 and EXT2 genes in 39 unrelated Spanish patients, most of them with moderate phenotype, and looked for genotype-phenotype correlations. We found the mutant allele in 37 patients, 29 in EXT1 and 8 in EXT2. Five of the EXT1 mutations were deletions identified by MLPA. Two cases of mosaicism were documented. We detected a lower number of exostoses in patients with missense mutation versus other kinds of mutations. In conclusion, we found a mutation in EXT1 or in EXT2 in 95% of the Spanish patients. Eighteen of the mutations were novel

Building genomic infrastructure: Sequencing platinum-standard reference-quality genomes of all cetacean species.

International audienc

No distinct local cuisines among humpback whales: A population diet comparison in the Southern Hemisphere

Southern hemisphere humpback whale (Megaptera novaeangliae, SHHW) breeding populations follow a high-fidelity Antarctic krill (Euphausia superba) diet while feeding in distinct sectors of the Southern Ocean. Their capital breeding life history requires predictable ecosystem productivity to fuel migration and migration-related behaviours. It is therefore postulated that populations feeding in areas subject to the strongest climate change impacts are more likely to show the first signs of a departure from a high-fidelity krill diet. We tested this hypothesis by investigating blubber fatty acid profiles and skin stable isotopes obtained from five SHHW populations in 2019, and comparing them to Antarctic krill stable isotopes sampled in three SHHW feeding areas in the Southern Ocean in 2019. Fatty acid profiles and δ13C and δ15N varied significantly among all five populations, however, calculated trophic positions did not (2.7 to 3.1). Similarly, fatty acid ratios, 16:1ω7c/16:0 and 20:5ω3/22:6ω3 were above 1, showing that whales from all five populations are secondary heterotrophs following an omnivorous diet with a diatom-origin. Thus, evidence for a potential departure from a high-fidelity Antarctic krill diet was not seen in any population. δ13C of all populations were similar to δ13C of krill sampled in productive upwelling areas or the marginal sea-ice zone. Consistency in trophic position and diet origin but significant fatty acid and stable isotope differences demonstrate that the observed variability arises at lower trophic levels. Our results indicate that, at present, there is no evidence of a divergence from a high-fidelity krill diet. Nevertheless, the characteristic isotopic signal of whales feeding in productive upwelling areas, or in the marginal sea-ice zone, implies that future cryosphere reductions could impact their feeding ecology

Basophil-lineage commitment in acute promyelocytic leukemia predicts for severe bleeding after starting therapy

Severe hemorrhagic events occur in a significant fraction of acute promyelocytic leukemia patients, either at presentation and/or early after starting therapy, leading to treatment failure and early deaths. However, identification of independent predictors for high-risk of severe bleeding at diagnosis, remains a challenge. Here, we investigated the immunophenotype of bone marrow leukemic cells from 109 newly diagnosed acute promyelocytic leukemia patients, particularly focusing on the identification of basophil-related features, and their potential association with severe bleeding episodes and patient overall survival. From all phenotypes investigated on leukemic cells, expression of the CD203c and/or CD22 basophil-associated markers showed the strongest association with the occurrence and severity of bleeding (p ≤ 0.007); moreover, aberrant expression of CD7, coexpression of CD34+/CD7+ and lack of CD71 was also more frequently found among patients with (mild and severe) bleeding at baseline and/or after starting treatment (p ≤ 0.009). Multivariate analysis showed that CD203c expression (hazard ratio: 26.4; p = 0.003) and older age (hazard ratio: 5.4; p = 0.03) were the best independent predictors for cumulative incidence of severe bleeding after starting therapy. In addition, CD203c expression on leukemic cells (hazard ratio: 4.4; p = 0.01), low fibrinogen levels (hazard ratio: 8.8; p = 0.001), older age (hazard ratio: 9.0; p = 0.002), and high leukocyte count (hazard ratio: 5.6; p = 0.02) were the most informative independent predictors for overall survival. In summary, our results show that the presence of basophil-associated phenotypic characteristics on leukemic cells from acute promyelocytic leukemia patients at diagnosis is a powerful independent predictor for severe bleeding and overall survival, which might contribute in the future to (early) risk-adapted therapy decisions.This work was supported by the Fundación Científica de la Asociación Española Contra el Cáncer (AECC, Madrid, Spain) and the Fundación Rafael del Pino (Madrid, Spain) and both CIBERONC (CB16/12/00400, CB16/12/00233, CB16/12/00480) and grant PI16/00787 from Instituto de Salud Carlos III (Ministerio de Economía y Competitividad, Madrid, Spain)

Identification and characterization of Cardiac Glycosides as senolytic compounds

Compounds with specific cytotoxic activity in senescent cells, or senolytics, support the causal involvement of senescence in aging and offer therapeutic interventions. Here we report the identification of Cardiac Glycosides (CGs) as a family of compounds with senolytic activity. CGs, by targeting the Na+/K+ATPase pump, cause a disbalanced electrochemical gradient within the cell causing depolarization and acidification. Senescent cells present a slightly depolarized plasma membrane and higher concentrations of H+, making them more susceptible to the action of CGs. These vulnerabilities can be exploited for therapeutic purposes as evidenced by the in vivo eradication of tumors xenografted in mice after treatment with the combination of a senogenic and a senolytic drug. The senolytic effect of CGs is also effective in the elimination of senescence-induced lung fibrosis. This experimental approach allows the identification of compounds with senolytic activity that could potentially be used to develop effective treatments against age-related diseases.We thank Matthias Drosten, Alejo Efeyan and Sean Morrison for plasmids. F.T-M. is a postdoctoral fellow from CONACYT (cvu 268632); P.P. is a predoctoral fellow from Xunta de Galicia; M.C. is a "Miguel Servet II" investigator (CPII16/00015). P.P.-R. receives support from a program by the Deputacion de Coruna (BINV-CS/2019). Work in the laboratory of M.C. is funded by grant RTI2018-095818-B-100 (MCIU/AEI/FEDER, UE). P.J.F.-M. is funded by the IMDEA Food Institute, the Ramon Areces Foundation, (CIVP18A3891), and a Ramon y Cajal Award (MICINN) (RYC-2017-22335). M.P.I. is funded by Talento Modalidad-1 Program Grant, Madrid Regional Government (#2018-T1/BIO-11262). F.P. was funded by a Long Term EMBO Fellowship (ALTF-358-2017) and F.H-G. was funded by the PhD4MD Programme of the IRB, Hospital Clinic and IDIBAPS. Work in the laboratory of M.S. was funded by the IRB and by grants from the Spanish Ministry of Economy co-funded by the European Regional Development Fund (ERDF) (SAF2013-48256-R), the European Research Council (ERC-2014-AdG/669622), and "laCaixa" Foundation.S