Lateral differentiation of Albeluvisols under the impact of subsurface drainage

Albeluvisols are temporarily waterlogged due to the argillic horizon that limits downward movement of rainfall water. These soils are hence frequently drained for cropping. Drainage modifies water movement in both direction and velocity, inducing a gradient in waterlogging conditions perpendicularly to the drain. Over time, it may induce a lateral differentiation of the soil solid phase with the distance from the drain. This study aims at characterising and quantifying this differentiation. Albeluvisols are characterised by the following horizon succession: A, Eg&BT, BTgd. The two last horizons exhibit a complex juxtaposition of white-grey, ochre and pale-brown volumes, and numerous black concretions or impregnations. In order to study the impact of drainage on the evolution of such soils, we have to characterise the soil differentiation perpendicularly to the drain by quantifying changes in the quality and the abundance of the different pedological volumes

Universal scattering behavior of co-assembled nanoparticle-polymer clusters

Water-soluble clusters made from 7 nm inorganic nanoparticles have been investigated by small-angle neutron scattering. The internal structure factor of the clusters was derived and exhibited a universal behavior as evidenced by a correlation hole at intermediate wave-vectors. Reverse Monte-Carlo calculations were performed to adjust the data and provided an accurate description of the clusters in terms of interparticle distance and volume fraction. Additional parameters influencing the microstructure were also investigated, including the nature and thickness of the nanoparticle adlayer.Comment: 5 pages, 4 figures, paper published in Physical Review

The dominant role of structure for solute transport in soil: experimental evidence and modelling of structure and transport in a field experiment

International audienceA classical transport experiment was performed in a field plot of 2.5 m2 using the dye tracer brilliant blue. The measured tracer distribution demonstrates the dominant role of the heterogeneous soil structure for solute transport. As with many other published experiments, this evidences the need of considering the macroscopic structure of soil to predict flow and transport. We combine three different approaches to represent the relevant structure of the specific situation of our experiment: i) direct measurement, ii) statistical description of heterogeneities and iii) a conceptual model of structure formation. The structure of soil layers was directly obtained from serial sections in the field. The sub-scale heterogeneity within the soil horizons was modelled through correlated random fields with estimated correlation lengths and anisotropy. Earthworm burrows played a dominant role at the transition between the upper soil horizon and the subsoil. A model based on percolation theory is introduced that mimics the geometry of earthworm burrow systems. The hydraulic material properties of the different structural units were obtained by direct measurements where available and by a best estimate otherwise. From the hydraulic structure, the 3-dimensional velocity field of water was calculated by solving Richards' Equation and solute transport was simulated. The simulated tracer distribution compares reasonably well with the experimental data. We conclude that a rough representation of the structure and a rough representation of the hydraulic properties might be sufficient to predict flow and transport, but both elements are definitely required

Impact of drainage on soil-forming mechanisms in a French Albeluvisol: Input of mineralogical data in mass-balance modelling

International audienceResearch on soil pedogenesis has mainly focused on the long-term soil formation and has most often neglected recent soil evolutions in response to human practices. Such recent soil evolutions are however of considerable interest to study the timing of soil forming processes in response to changes in environmental conditions. In this paper, we model the Albeluvisol evolution in response to agricultural drainage. This was considered as a model case to study the velocity of mineralogical changes in soil as a result of eluviation and redox processes. We used a space-for-time substitution approach in combination with mass balance modelling based on mineralogical data in order to identify and characterise the mineralogical transformations responsible for the recent soil evolution in response to subsurface drainage. This approach allowed demonstrating that the main effects of subsurface drainage are (i) increasing precipitation of Mn oxides and Mn-rich ferrihydrite with decreasing distance to the drain as a result of the change in redox conditions and (ii) increasing loss of clay-sized oxides and smectites due to the enhanced eluviation in the vicinity of the drain. Both processes induce significant matter fluxes in comparison with those that occurred over the long-term soil formation. Nowadays, the precipitation of Mn oxides and Mn-rich ferrihydrite seems to still be active in the studied soil. On the opposite, the eluviation process appears less active than immediately after the drainage network installation, if not totally inactive. It thus demonstrates that some soil processes may have significant impact on the soil mineralogical composition even if they are only active over very short periods of time after a change in environmental conditions

Apatites in Gale Crater

ChemCam is an active remote sensing instrument suite that has operated successfully on MSL since landing Aug. 6th, 2012. It uses laser pulses to remove dust and to analyze rocks up to 7 m away. Laser-induced breakdown spectroscopy (LIBS) obtains emission spectra of materials ablated from the samples in electronically excited states. The intensities of the emission lines scale with the abundances of the related element. ChemCam is sensitive to most major rock-forming elements as well as to a set of minor and trace elements such as F, Cl, Li, P, Sr, Ba, and Rb. The measured chemical composition can then be used to infer the mineralogical composition of the ablated material. Here, we report a summary of inferred apatite detections along the MSL traverse at Gale Crater. We present the geologic settings of these findings and derive some interpretations about the formation conditions of apatite in time and space

Activity-Dependent Bulk Endocytosis and Clathrin-Dependent Endocytosis Replenish Specific Synaptic Vesicle Pools in Central Nerve Terminals

Multiple synaptic vesicle (SV) retrieval modes exist in central nerve terminals to maintain a continual supply of SVs for neurotransmission. Two such modes are clathrin-mediated endocytosis (CME), which is dominant during mild neuronal activity and activity-dependent bulk endocytosis (ADBE), which is dominant during intense neuronal activity. However little is known about how activation of these SV retrieval modes impact on the replenishment of the total SV recycling pool and the pools that reside within it; the readily releasable pool (RRP) and reserve pool. To address this question, we examined the replenishment of all three SV pools by triggering these SV retrieval modes during both high and low intensity stimulation of primary rat neuronal cultures. SVs generated by CME and ADBE were differentially labelled using the dyes FM1-43 and FM2-10 and their replenishment of specific SV pools was quantified using stimulation protocols that selectively depleted each pool. Our studies indicate that while the RRP was replenished by CME-generated SVs, ADBE provided additional SVs to increase the capacity of the reserve pool. Morphological analysis of the uptake of the fluid phase marker horse radish peroxidase corroborated these findings. The differential replenishment of specific SV pools by independent SV retrieval modes illustrates how previously experienced neuronal activity impacts on the capability of central nerve terminals to respond to future stimuli

Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters

RNA polymerase II (Pol II) small nuclear RNA (snRNA) promoters and type 3 Pol III promoters have highly similar structures; both contain an interchangeable enhancer and "proximal sequence element" (PSE), which recruits the SNAP complex (SNAPc). The main distinguishing feature is the presence, in the type 3 promoters only, of a TATA box, which determines Pol III specificity. To understand the mechanism by which the absence or presence of a TATA box results in specific Pol recruitment, we examined how SNAPc and general transcription factors required for Pol II or Pol III transcription of SNAPc-dependent genes (i.e., TATA-box-binding protein [TBP], TFIIB, and TFIIA for Pol II transcription and TBP and BRF2 for Pol III transcription) assemble to ensure specific Pol recruitment. TFIIB and BRF2 could each, in a mutually exclusive fashion, be recruited to SNAPc. In contrast, TBP-TFIIB and TBP-BRF2 complexes were not recruited unless a TATA box was present, which allowed selective and efficient recruitment of the TBP-BRF2 complex. Thus, TBP both prevented BRF2 recruitment to Pol II promoters and enhanced BRF2 recruitment to Pol III promoters. On Pol II promoters, TBP recruitment was separate from TFIIB recruitment and enhanced by TFIIA. Our results provide a model for specific Pol recruitment at SNAPc-dependent promoters

Involvement of angiotensin II in the remodeling induced by a chronic decrease in blood flow in rat mesenteric resistance arteries

Blood flow reduction induces inward remodeling of resistance arteries (RAs). This remodeling occurs in ischemic diseases, diabetes and hypertension. Nonetheless, the effect of flow reduction per se, independent of the effect of pressure or metabolic influences, is not well understood in RA. As angiotensin II is involved in the response to flow in RA, we hypothesized that angiotensin II may also be involved in the remodeling induced by a chronic flow reduction. We analyzed the effect of angiotensin I-converting enzyme inhibition (perindopril) and angiotensin II type 1 receptor blockade (candesartan) on inward remodeling induced by blood flow reduction in vivo in rat mesenteric RAs (low flow (LF) arteries). After 1 week, diameter reduction in LF arteries was associated with reduced endothelium-dependent relaxation and lower levels of eNOS expression. Superoxide production and extracellular signal-regulated kinases 1/2 (ERK1/2 phosphorylation were higher in LF than in normal flow arteries. Nevertheless, the absence of eNOS or superoxide level reduction (tempol or apocynin) did not prevent LF remodeling. Perindopril and candesartan prevented inward remodeling in LF arteries. Contractility to angiotensin II was reduced in LF vessels by perindopril, candesartan and the ERK1/2 blocker PD98059. ERK1/2 activation (ratio phospho-ERK/ERK) was higher in LF arteries, and this activation was prevented by perindopril and candesartan. ERK1/2 inhibition in vivo (U0126) prevented LF-induced diameter reduction. Thus, inward remodeling because of blood flow reduction in mesenteric RA depends on unopposed angiotensin II-induced contraction and ERK1/2 activation, independent of superoxide production. These findings might be of importance in the treatment of vascular disorders