The effect of vascular graft and human umbilical cord blood-derived CD34+ stem cell on peripheral nerve healing

AIM: There are many trials concerning peripheral nerve damage causes and treatment options. Unfortunately, nerve damage is still a major problem regarding health, social and economic issues. On this study, we used vascular graft and human cord blood derived stem cells to find an alternative treatment solution to this problem. MATERIAL AND METHODS: We used 21 female Wistar rats on our study. They were anesthetized with ketamine and we studied right hind limbs. On Group 1, we did a full layer cut on the right sciatic nerve. On Group 2, we did a full layer cut on the right sciatic nerve, and we covered synthetic vascular graft on cut area. On Group 3, we did a full layer cut on right sciatic nerve, and we covered the area with stem cell applied vascular graft. RESULTS: At the end of postoperative 8. weeks, we performed EMG on the rats. When we compared healthy and degenerated areas as a result of EMG, we found significant amplitude differences between the groups on healthy areas whereas there was no significant difference on degenerated areas between the groups. Then we re-opened the operated area again to reveal the sciatic nerve cut area, and we performed electron microscope evaluation. On the stem cell group, we observed that both the axon and the myelin sheet prevented degeneration. CONCLUSION: This study is a first on using synthetic vascular graft and cord blood derived CD34+ cells in peripheral nerve degeneration. On the tissues that were examined with electron microscope, we observed that CD34+ cells prevented both axonal and myelin sheath degeneration. Nerve tissue showed similar results to the control group, and the damage was minimal. © 2018 Ali Yilmaz, Abdullah Topcu, Cagdas Erdogan, Levent Sinan Bir, Barbaros Sahin, Gulcin Abban, Erdal Coskun, Ayca Ozkul

Time out: An evaluation of rebound facilities

Challenging or provocative student behavior, such as talking back to the teacher, or disruptive classroom behavior, is an issue for schools around the world. In the Netherlands there is a growing need for school time-out programs that are meant to cater to the needs of students who have been expelled due to their maladaptive behavior and are not accepted in another school for this same reason. The most common school time-out program in the Netherlands is called Rebound. Rebounds are meant to achieve two goals: (1) care and interventions for students who are referred, and (2) a positive and safe school climate for their non-referred peers. Using comparative empirical approaches, this study focuses on the fit between referred students’ characteristics and the program offered in Rebounds, the effects of Rebound facilities on students’ social adjustment, and the association between leisure activities and school adjustment.Samenwerkingsverband Zuid-Holland WestDevelopment Psychopathology in context: clinical setting

Effect of different levels of royal jelly on biochemical parameters of swimmers

This study aims to investigate the effects of different levels of royal jelly supplementation on biochemical parameters in swimmers. Randomly selected 40 male swimmers aged 18 to 25 years attending the same trainings were recruited. Swimmers were assigned to 4 groups each with 10 subjects. Varying amounts of royal jelly (2, 1 g and 500 mg) were given to the 1st, 2nd and 3rd groups and placebo (corn starch) to the 4th group. Participants were trained by swimming totally 20 km in 2 h on 5 days a week for 4 weeks. Resting blood samples were taken before royal jelly administration and after 30 days of application. Then biochemical analyses were performed. Different levels of royal jelly were found to be ineffective on glucose, total cholesterol, high density lipoprotein (HDL) cholesterol, low density lipoprotein (LDL) cholesterol, lactate dehydrogenase (LDH), creatine kinase (CK), aspartate aminotransferase (AST), alanine aminotransferase (ALT) levels of the swimmers. Blood urea nitrogen (BUN) and creatinine levels increased after the training program, and BUN level was higher in the group receiving 500 mg royal jelly than those in the other groups. The increment in creatinine levels was higher in those groups receiving higher amounts of royal jelly after the training. A supplementation of 500 mg, 1 and 2 g/day of royal jelly throughout the 30 day-exercise program was not significantly effective in the swimmers. Also, due to its high amino acid content, BUN and creatinine levels tended to increase.Key words: Royal jelly, swimming, exercise, biochemical parameters, ergogenic aids

Parasitic Appendicitis From Past to Present in Turkey

Background: Understanding the etiology of appendicitis is important for developing effective treatments the rela­tionship between parasitic appendicitis and various socio-cultural factors were examined, particu­larly with respect to the incidence of literacy. The aim of the article was to research the relations between para­sitic appendicitis and literacy ratio in population.Methods: Cases of parasitic appendicitis resulting in surgery performed at Buca Seyfi Demirsoy Large State Hospital Surgery Clinic between 2002 and 2009 were retrospectively reviewed and classified accord­ing to age, sex, type of parasite, morbidity, and mortality. Studies conducted in different regions of Turkey as well as in other countries were reviewed to determine if there was a relationship between para­sitic appendicitis and literacy. Results: Of the 1,969 appendectomy cases reviewed, nine were classified as parasitic appendicitis (0.45%). Enterobius vermicularis was observed in seven cases and Taenia spp. in two. The average age was 26.4 yr. No morbidity or mortality was found.Conclusion: The data were compared with a retrospective review of studies conducted in the same regions and a decrease in the rate of parasitic appendicitis was observed during the period between the two re­views. It was determined that a low literacy rate was associated with an increase in the incidence of para­sitic appendicitis. Observations made between different countries also produced similar results. In coun­tries where the incidence of parasitic appendiciticis was greater than 1.5%, the literacy rate was less than 88%. To avoid appendectomy resulting from parasites, it is important to increase education and literacy. In some areas, individuals with appendicitis undergo surgery due to a lack of education or poor literacy

Distribution of mast cells in lung tissues of rats exposed to biomass smoke

This study was designed to evaluate the distribution of mast cells in the lung tissues of rats exposed to biomass  smoke. Fifty six female Wistar albino adult rats were used. They were divided into two experimental groups  (control and biomass smoke-treated), each containing 28 animals. Control rats were not exposed to the  biomass smoke at any time during the experiment. Rats in the treatment group were exposed daily (one hour)  to biomass smoke for 3, 6 or 9 months. Lung tissues samples were obtained under deep anesthesia from the  randomly selected 7 animals in both groups. Lung tissues were fixed in Mota’s fixative (BLA) for 24 h and  embedded in paraffin. Sections of 6 μm thickness were cut and stained with 0.5% toluidine blue in 0.5 N  hydrochloric acid at pH 0.5 for 30 min. The numbers of mast cell in lung tissues of the animals exposed to  the biomass for 6 or 9 months were significantly (P<0.05) higher than controls. This study showed that long  term exposure to biomass smoke was associated with the increased number of mast cells in the lung.

Evaluation of Probabilistic Streamflow Forecasts Based on EPS for a Mountainous Basin in Turkey

AbstractWhen designing water structures or managing a watershed it is a challenging task to determine the response of a basin to storm and/or snowmelt. In this study, the Upper Euphrates Basin (10,275 km2 area and elevation range of 1125-3500 m) located at the headwater of Euphrates River, one of Turkey's most important rivers, is selected as the application area. In this region, snowmelt runoff constitutes approximately 2/3 in volume of the total yearly runoff, therefore, runoff modeling and forecasting during spring and early summer is important in terms of energy and water resources management. The aim of the study is to make a forward-oriented, medium-range flow forecasting using Ensemble Prediction System (EPS) which is a pioneer study for Turkey. Conceptual hydrological model HBV, which has a common usage in the literature, is chosen to predict streamflows. According to the results, Nash-Sutcliffe model efficiencies are 0.85 for calibration (2001-2008) and 0.71 for validation (2009-2014) respectively. After calibrating/validating the hydrologic model, EPS data including 51 different combinations produced by ECMWF is used as probability based weather forecasts. Melting period during March-June of 2011 is chosen as the forecast period. The probabilistic skill of EPS based hydrological model results are analyzed to verify the ensemble forecasts

Seismic data reveal eastern Black Sea Basin structure

Rifted continental margins are formed by progressive extension of the lithosphere. The development of these margins plays an integral role in the plate tectonic cycle, and an understanding of the extensional process underpins much hydrocarbon exploration. A key issue is whether the lithosphere extends uniformly, or whether extension varies\ud with depth. Crustal extension may be determined using seismic techniques. Lithospheric extension may be inferred from the waterloaded subsidence history, determined from\ud the pattern of sedimentation during and after rifting. Unfortunately, however, many rifted margins are sediment-starved, so the subsidence history is poorly known.\ud To test whether extension varies between the crust and the mantle, a major seismic experiment was conducted in February–March 2005 in the eastern Black Sea Basin (Figure 1), a deep basin where the subsidence history is recorded\ud by a thick, post-rift sedimentary sequence. The seismic data from the experiment indicate the presence of a thick, low-velocity zone, possibly representing overpressured sediments. They also indicate that the basement and\ud Moho in the center of the basin are both several kilometers shallower than previously inferred. These initial observations may have considerable impact on thermal models of the petroleum system in the basin. Understanding\ud the thermal history of potential source rocks is key to reducing hydrocarbon exploration risk. The experiment, which involved collaboration between university groups in the United Kingdom, Ireland, and Turkey, and BP and\ud Turkish Petroleum (TPAO), formed part of a larger project that also is using deep seismic reflection and other geophysical data held by the industry partners to determine the subsidence history and hence the strain evolution of\ud the basin

A task and performance analysis of endoscopic submucosal dissection (ESD) surgery

BACKGROUND: ESD is an endoscopic technique for en bloc resection of gastrointestinal lesions. ESD is a widely-used in Japan and throughout Asia, but not as prevalent in Europe or the US. The procedure is technically challenging and has higher adverse events (bleeding, perforation) compared to endoscopic mucosal resection. Inadequate training platforms and lack of established training curricula have restricted its wide acceptance in the US. Thus, we aim to develop a Virtual Endoluminal Surgery Simulator (VESS) for objective ESD training and assessment. In this work, we performed task and performance analysis of ESD surgeries. METHODS: We performed a detailed colorectal ESD task analysis and identified the critical ESD steps for lesion identification, marking, injection, circumferential cutting, dissection, intraprocedural complication management, and post-procedure examination. We constructed a hierarchical task tree that elaborates the order of tasks in these steps. Furthermore, we developed quantitative ESD performance metrics. We measured task times and scores of 16 ESD surgeries performed by four different endoscopic surgeons. RESULTS: The average time of the marking, injection, and circumferential cutting phases are 203.4 (σ: 205.46), 83.5 (σ: 49.92), 908.4 s. (σ: 584.53), respectively. Cutting the submucosal layer takes most of the time of overall ESD procedure time with an average of 1394.7 s (σ: 908.43). We also performed correlation analysis (Pearson's test) among the performance scores of the tasks. There is a moderate positive correlation (R = 0.528, p = 0.0355) between marking scores and total scores, a strong positive correlation (R = 0.7879, p = 0.0003) between circumferential cutting and submucosal dissection and total scores. Similarly, we noted a strong positive correlation (R = 0.7095, p = 0.0021) between circumferential cutting and submucosal dissection and marking scores. CONCLUSIONS: We elaborated ESD tasks and developed quantitative performance metrics used in analysis of actual surgery performance. These ESD metrics will be used in future validation studies of our VESS simulator

Transcriptional down-regulation of ccr5 in a subset of HIV+ controllers and their family members.

HIV +Elite and Viremic controllers (EC/VCs) are able to control virus infection, perhaps because of host genetic determinants. We identified 16% (21 of 131) EC/VCs with CD4 +T cells with resistance specific to R5-tropic HIV, reversed after introduction of ccr5. R5 resistance was not observed in macrophages and depended upon the method of T cell activation. CD4 +T cells of these EC/VCs had lower ccr2 and ccr5 RNA levels, reduced CCR2 and CCR5 cell-surface expression, and decreased levels of secreted chemokines. T cells had no changes in chemokine receptor mRNA half-life but instead had lower levels of active transcription of ccr2 and ccr5, despite having more accessible chromatin by ATAC-seq. Other nearby genes were also down-regulated, over a region of ~500 kb on chromosome 3p21. This same R5 resistance phenotype was observed in family members of an index VC, also associated with ccr2/ccr5 down-regulation, suggesting that the phenotype is heritable