The Effects of Different Diet Models on Oocyte Quality, Oocyte Maturation and in Vitro Fertilization Outcomes in Mice

Nutritional quality affects fertility in both men and women. Nutrition should be considered in the prevention and treatment of infertility in men and women. In this study, we evaluated IVF rates, oocyte maturation and oocyte quality in a female animal model feding ketogenic, western and standard type diet. Female BALB / c mice were allocated to three groups: (a) control; (b) Western and (c) Ketogenic. After feeding female mice with these 3 feeding groups for 4 weeks, IVF was administered before sacrification of the mice. The number of collected oocytes and oocyte quality, fertilization rates and embryo quality were evaluated.The number of total oocytes was the highest in the ketogenic diet and the least in the western diet. MII stage oocytes in the ketogenic group were statistically significantly higher than the control group and western groups. Fertilized oocyte count was significantly lower in the western group compared to the other two groups. When the two-cell zygote stage was evaluated, it was found that the results were better in the ketogenic diet group and worse in the western diet group. The worst results in terms of the number of zygotes reaching the blastocyst stage were observed in the western type nutrition group, and the best results were observed in the group on the keteogenic diet. The results of our study show that western nutritional models negatively affect IVF results, while ketogenic diet has a positive effect. Keywords: Fertility, ketogenic diet, oocyte, ovulation, western diet DOI: 10.7176/JMPB/72-02 Publication date: January 31st 202

Evaluation of patients with fibrotic interstitial lung disease: Preliminary results from the Turk-UIP study

OBJECTIVE: Differential diagnosis of idiopathic pulmonary fibrosis (IPF) is important among fibrotic interstitial lung diseases (ILD). This study aimed to evaluate the rate of IPF in patients with fibrotic ILD and to determine the clinical-laboratory features of patients with and without IPF that would provide the differential diagnosis of IPF. MATERIAL AND METHODS: The study included the patients with the usual interstitial pneumonia (UIP) pattern or possible UIP pattern on thorax high-resolution computed tomography, and/or UIP pattern, probable UIP or possible UIP pattern at lung biopsy according to the 2011 ATS/ERSARS/ALAT guidelines. Demographics and clinical and radiological data of the patients were recorded. All data recorded by researchers was evaluated by radiology and the clinical decision board. RESULTS: A total of 336 patients (253 men, 83 women, age 65.8 +/- 9.0 years) were evaluated. Of the patients with sufficient data for diag-nosis (n=300), the diagnosis was IPF in 121 (40.3%), unclassified idiopathic interstitial pneumonia in 50 (16.7%), combined pulmonary fibrosis and emphysema (CPFE) in 40 (13.3%), and lung involvement of connective tissue disease (CTD) in 16 (5.3%). When 29 patients with definite IPF features were added to the patients with CPFE, the total number of IPF patients reached 150 (50%). Rate of male sex (p<0.001), smoking history (p<0.001), and the presence of clubbing (p=0.001) were significantly high in patients with IPE None of the women <50 years and none of the men <50 years of age without a smoking history were diagnosed with IPE Presence of at least 1 of the symptoms suggestive of CTD, erythrocyte sedimentation rate (ESR), and antinuclear antibody (FANA) positivity rates were significantly higher in the non-IPF group (p<0.001, p=0.029, p=0.009, respectively). CONCLUSION: The rate of IPF among patients with fibrotic ILD was 50%. In the differential diagnosis of IPF, sex, smoking habits, and the presence of clubbing are important. The presence of symptoms related to CTD, ESR elevation, and EANA positivity reduce the likelihood of IPF

Volume CXIV, Number 4, November 7, 1996

Objective: Turner syndrome (TS) is a chromosomal disorder caused by complete or partial X chromosome monosomy that manifests various clinical features depending on the karyotype and on the genetic background of affected girls. This study aimed to systematically investigate the key clinical features of TS in relationship to karyotype in a large pediatric Turkish patient population.Methods: Our retrospective study included 842 karyotype-proven TS patients aged 0-18 years who were evaluated in 35 different centers in Turkey in the years 2013-2014.Results: The most common karyotype was 45,X (50.7%), followed by 45,X/46,XX (10.8%), 46,X,i(Xq) (10.1%) and 45,X/46,X,i(Xq) (9.5%). Mean age at diagnosis was 10.2±4.4 years. The most common presenting complaints were short stature and delayed puberty. Among patients diagnosed before age one year, the ratio of karyotype 45,X was significantly higher than that of other karyotype groups. Cardiac defects (bicuspid aortic valve, coarctation of the aorta and aortic stenosis) were the most common congenital anomalies, occurring in 25% of the TS cases. This was followed by urinary system anomalies (horseshoe kidney, double collector duct system and renal rotation) detected in 16.3%. Hashimoto's thyroiditis was found in 11.1% of patients, gastrointestinal abnormalities in 8.9%, ear nose and throat problems in 22.6%, dermatologic problems in 21.8% and osteoporosis in 15.3%. Learning difficulties and/or psychosocial problems were encountered in 39.1%. Insulin resistance and impaired fasting glucose were detected in 3.4% and 2.2%, respectively. Dyslipidemia prevalence was 11.4%.Conclusion: This comprehensive study systematically evaluated the largest group of karyotype-proven TS girls to date. The karyotype distribution, congenital anomaly and comorbidity profile closely parallel that from other countries and support the need for close medical surveillance of these complex patients throughout their lifespa

Impact of opioid-free analgesia on pain severity and patient satisfaction after discharge from surgery: multispecialty, prospective cohort study in 25 countries

Background: Balancing opioid stewardship and the need for adequate analgesia following discharge after surgery is challenging. This study aimed to compare the outcomes for patients discharged with opioid versus opioid-free analgesia after common surgical procedures.Methods: This international, multicentre, prospective cohort study collected data from patients undergoing common acute and elective general surgical, urological, gynaecological, and orthopaedic procedures. The primary outcomes were patient-reported time in severe pain measured on a numerical analogue scale from 0 to 100% and patient-reported satisfaction with pain relief during the first week following discharge. Data were collected by in-hospital chart review and patient telephone interview 1 week after discharge.Results: The study recruited 4273 patients from 144 centres in 25 countries; 1311 patients (30.7%) were prescribed opioid analgesia at discharge. Patients reported being in severe pain for 10 (i.q.r. 1-30)% of the first week after discharge and rated satisfaction with analgesia as 90 (i.q.r. 80-100) of 100. After adjustment for confounders, opioid analgesia on discharge was independently associated with increased pain severity (risk ratio 1.52, 95% c.i. 1.31 to 1.76; P &lt; 0.001) and re-presentation to healthcare providers owing to side-effects of medication (OR 2.38, 95% c.i. 1.36 to 4.17; P = 0.004), but not with satisfaction with analgesia (beta coefficient 0.92, 95% c.i. -1.52 to 3.36; P = 0.468) compared with opioid-free analgesia. Although opioid prescribing varied greatly between high-income and low- and middle-income countries, patient-reported outcomes did not.Conclusion: Opioid analgesia prescription on surgical discharge is associated with a higher risk of re-presentation owing to side-effects of medication and increased patient-reported pain, but not with changes in patient-reported satisfaction. Opioid-free discharge analgesia should be adopted routinely

Investigation of beta globin gene mutations in Syrian refugee patients

Objectives: This study, detection of beta globin gene mutations in thalassemia major patients who migrated from Syria to Kahramanmaraş region were planned. Materials and methods: The study included 35 Syrian national beta thalassemia major patients. Beta globin gene mutations were detected by ARMS (Amplification Refractory Mutation System) method, RFLP (Restriction Fragment Length Polymorphism) method and DNA sequence analysis. Codon 15, codon 9/10, codon 5 and codon 8 mutations, which we could not detect with other methods in our study, were detected by sequence analysis. Results: In beta thalassemia major patients, 16 types of mutations were detected, the most common being IVS-I- 110 (n = 8). Other mutations are according to frequency order IVS-II-745 (n = 3), codon 44 (n = 3), codon 15 (n = 3), IVS-I-110/IVS-I-1 (n = 3), codon 5 (n = 2), IVS-I-1 (n = 2), codon 8/IVS-II-1 (n = 2), codon 44/codon 15 (n = 2), IVS-II-1 (n = 1), codon 39 (n = 1), IVS-I-6/codon 5 (n = 1), codon 9/10 (n = 1), IVS-I-110/codon 39 (n = 1), IVS-I-5/IVS-II-1 (n = 1), codon 39/IVS-II-745 (n = 1). Conclusions: According to the results of our study betathalassemia mutations in Syrian immigrant groups show heterogeneity and mutation types of mutation map is similar to Turkey. The conclusion is to prevent families to have a second patient child by genetic counseling

Measles-rubella-mumps vaccination in cases with egg allergy: Is skin prick test necessary? Can vaccination be done safely?

WOS: 000418200900008Amaç: İçerisinde yumurta proteini bulunan kızamık-kızamıkçık-kabakulak aşısının (KKK) yumurta alerjisi olan hastalarda doğrudan uygulanması önerilmesine karşın literatürde aşı uygulaması sırasında anaflaksi gözlenen olguların bulunması nedeniyle bu konu hem hekim hem de ailelerde sıkıntılara neden olmaktadır. Bu çalışmada, kliniğimizde yumurta alerjisi nedeniyle izlenen, KKK aşı uygulanmış hastalarda aşı sonrası reaksiyon sıklığının ve aşı uygulaması öncesi aşı ile deri prick test uygulamasının gerekliliğinin değerlendirilmesi amaçlanmıştır.Yöntem: Retrospektif kesitsel çalışmamızda Eylül 2013-Mayıs 2015 tarihleri arasında yumurta alerjisi tanısı ile izlenip, KKK aşısı uygulanmış olan 82 hasta değerlendirildi. Bulgular: Tanı anında yaş ortalaması 8,34±7,1ay olan 82 hastanın %37,8'i (n=31) kız idi. Tanısal dağılımına göre hastalar değerlendirildiğinde, %68,3'ü (n=56) atopik dermatit, %8,5'i (n=7) ürtiker/anjioödem, %18,3'ü (n=15) reaktif havayolu hastalığı, %4,9'u (n=4) anafilaksi olarak değerlendirilmişti. KKK aşı uygulaması öncesi %21'ine (n=17) aşı ile deri prick test uygulanmıştı. Anafilaksi tanısı ile izlenen 2 olgu dışında aşı tam doz yapılmış olup, olguların hiçbirinde KKK aşısı uygulaması sonrasında reaksiyon gözlenmemişti. Sonuç: Yumurta alerjili hastalarda KKK aşısı sonrası herhangi bir reaksiyon gözlenmemiştir. Bu hastalarda diğer sağlıklı çocuklardan farklı reaksiyonlar gözlemediğimiz için KKK aşısının diğer aşıların yapıldığı merkezlerde, aşı ile teste ve bölünmüş dozlara gerek kalmadan uygulanmasında sakınca olmadığını düşünüyoruz.Objective: Measles-mumps-rubella vaccine which contains egg protein is considered as a potential allergen in children with egg allergy and although administration of the vaccine without any evaluation in egg allergic patients is suggested, due to the presence of reported cases of anaphylaxis during vaccination in the literature this issue causes distress to both families and doctors. In this study the frequency of reactions observed after MMR vaccination and the necessity of skin prick test in the patients followed up with egg allergy in our clinic were evaluated. Methods: In our study, 82 patients followed up with the diagnosis of egg allergy diagnosis and administered MMR vaccine between September 2013 and May 2015 were included in retrospective cross-sectional study. Results: The average age was 8.34 +/- 7.1 months, and 37.8% (n=31) of the cases were female. According to their diagnostic distribution, the patients had atopic dermatitis (n=56; 68.3%) had, urticaria/angioedema (n=7; 8.5%), reactive airway disease (n=15; 18.3%), and anaphylaxis (n=9; 4.9%). Skin prick test was administered to 17 (21%) patients before MMR vaccination. A whole dose of vaccine was administered to all cases except in two cases with the diagnosis of anaphylaxis. There was no reaction observed after MMR vaccination in any of the cases. Conclusion: Any reaction was not observed in any of egg allergy cases after MMR vaccination. Since we didn't observe any reaction in these patients different from that seen in other healthy children, we think that there is no containdication for administering MMR vaccines at the centers in which the other vaccines were done, without any need to administer MMR vaccine with prick test or in divided doses

Effects of aspiration pneumonia on the intensive care requirements and in-hospital mortality of hospitalised patients with acute cerebrovascular disease

Introduction : In this study, we aimed to evaluate the effects of the development of aspiration pneumonia (AP) on the intensive care unit (ICU) requirements and in-hospital mortality of patients hospitalised in the neurology ward due to an acute cerebrovascular accident (CVA). Material and methods: Five hundred and three patients hospitalised in the neurology ward following an acute CVA were retrospectively analysed. The patients were divided into two groups: those with AP (group 1) and those without AP (group 2). Demographic characteristics and physical and radiological findings, including the localisation, lateralisation and aetiology of the infarction, in addition to ICU requirements and mortality, were evaluated. Results : Aspiration pneumonia was detected in 80 (15.9%) patients during the in-hospital stay. Transfer to the ICU for any reason was required in 37.5% of the patients in group 1 and 4.7% of those in group 2 (p < 0.001). In-hospital mortality occurred in 7.5% and 1.4% of the patients in group 1 and group 2, respectively (p = 0.006). The incidence of AP was highest in patients with an infarction of the medial cerebral artery (MCA) (p < 0.001). AP was associated with older age (p < 0.001), hypertension (p = 0.007), echocardiography findings (p = 0.032) and the modified Rankin Scale (mRS) score (p < 0.001). Conclusions : Our findings suggest that the requirement rate for transfer to the ICU and the mortality rate appear to be significantly higher in patients with a diagnosis of AP. Precautions should be taken, starting from the first day of hospitalisation, to decrease the incidence of AP in patients with acute CVA, focusing especially on older patients and those with a severe mRS score

Pediatrik Burkitt lenfomalarda miRNA-seq ile belirlenen miRNA seviyelerinin non-invazif tanı biyobelirteç potansiyelleri

Giriş: Pediatrik Burkitt lenfoma (pBL) çocuklarda en sık gözlemlenennon-Hodgkin lenfoma türüdür. Oldukça hızlı büyüyen ve agresif karakteristiğesahip pBL’nin tanısının hızlı biçimde konulup tedaviye kısa süredebaşlanması gerekmektedir. pBL tanısının konulabilmesi için uygulananve tümör biyopsi temini içeren invazif yöntemlerin yerini alabilecek noninvaziftanı yöntemlerine ihtiyaç vardır. miRNA’ların kanser hastalarınınplazma örneklerinde yüksek ifade edilmesi durumunda non-invazif tanıyıgeliştirme potansiyelleri bulunmaktadır. pBL hasta plazmalarındaki miRNA’larınifade profilleri ve pBL tanısını geliştirme potansiyelleri öncedenincelenmemiştir. Bu çalışmada pBL hasta plazmalarında ifadesi artan dolaşımsalserbest ve ekzozomal miRNA’lar miRNA-seq ile belirlenerek miRNAseviyelerinin non-invazif (invazif olmayan) tanı biyobelirteç potansiyellerideğerlendirilmiştir.Yöntem: Tanı esnasında 17 pBL olgusuna ait tümör doku ve çevreselkan örnekleri yanı sıra kontrol grubu olarak 15 obstrüktif uyku apnesi(OSA) olgusuna ait tonsil doku ve çevresel kan örnekleri temin edildi.FFPE tümör, dolaşımsal serbest ve ekzozomal miRNA izolasyonları uygunQiagen kitleriyle gerçekleştirildi. OSA tonsil dokularında IgD−/CD38+/CD77+ fenotipli sentroblast hücreleri FACS ile ayrıştırıldıktan sonra totalmiRNA örnekleri miRNeasy Micro Kit (Qiagen, Almanya) kullanılarak izoleedildi. Agilent 2100 BioAnalyzer cihazıyla miRNA örneklerinin boyutve konsantrasyonları değerlendirilerek NUGEN Ovation® SoLo RNA-SeqSystem ile miRNA-seq kütüphaneleri hazırlandı. NovaSeq 6000 yeni nesildizileme sistemi aracılığıyla tek uçlu, 50 bç uzunluğunda ve her bir miRNAörneği için ~10 milyon adet NGS okuması (İng. reads) elde edildi. Kontrolgrubuna kıyasla istatistiksel olarak anlamlı bir farkla ifade edilen miRNA’larmiRDeep2 programını içeren biyoinformatik iş akışı ile belirlendi. İfadesiartan miRNA’ların tanısal potansiyelleri easyROC (sürüm: 1.3.1) programıylaalıcı işletim karakteristiği (İng. receiver operating characteristic[ROC]) eğrisi altındaki alan (AUC) belirlenerek değerlendirildi. miRNA-seqsonuçlarının çapraz teyidi seçili miRNA’ların qRT-PCR deneyleri ve doğrusalregresyon analizleriyle gerçekleştirildi.Sonuçlar: pBL olgularındaki total dolaşımsal serbest miRNA konsantrasyonlarıkontrol grubu örneklere kıyasla anlamlı biçimde yüksekti. miRNAseqanalizleriyle tümör doku örneklerinde toplam 409 adet miRNA’nınifadesinin istatistiksel olarak anlamlı (log2FC&gt;1, FDR&lt;0.05) olarak arttığıbelirlendi. Plazma örneklerinde ise kontrol grubuna kıyasla 35 dolaşımsalserbest ve 128 dolaşımsal ekzozomal miRNA’nın ifadesinde artış gözlemlendi.pBL tümör dokularında ifadesi yüksek olan miRNA’ların ROC analizlerihsa-miR-143-5p, hsa-miR-654-3p, ve hsa-miR-10400-5p ifade seviyelerininpBL için tanısal biyobelirteçler olabileceğini gösterdi. pBL plazmalarındayüksek ifadeli dolaşımsal serbest ve ekzozomal miRNA’ların tekveya entegre ROC analizleri, özellikle dolaşımsal ekzozomal miRNA’larınbazı üçlü kombinasyonlarının non-invazif tanı geliştirme potansiyeline(AUC&gt;0.9) sahip olduğunu gösterdi (Şekil 1). Seçili miRNA’lar kullanılarakgerçekleştirilen qRT-PCR deneyleriyle miRNA-seq analiz sonuçlarının hemtümör doku miRNA hem de plazma miRNA seviyeleri için büyük ölçüdeörtüştüğü gözlemlendi.Tartışma: Sonuç olarak, bu projede pediatrik Burkitt lenfomanın invazifolmayan tanısını geliştirme potansiyeli bulunan ve plazma örneklerindeifadeleri artan miRNA’lar miRNA-seq aracılığıyla belirlenmiştir. Rutin klinikdeğerlendirmelerle birlikte pBL hasta plazmalarında ifadesi artmış ekzozomalmiRNA’lar hastanelerde qRT-PCR aracılığıyla tespit edilip hızlı tanıkonmasına yardımcı olabilir.Anahtar kelimeler: Pediatrik Burkitt lenfoma, invazif olmayan tanı, dolaşımsal serbestmiRNA, dolaşımsal ekzozomal miRNA, sıvı biyopsi.</p

Has the COVID-19 pandemic changed attitudes and behaviors concerning cosmetic care and procedures among patients presenting to the dermatology outpatient clinic? A multicenter study with 1437 participants

Background The rate of cosmetic procedures and use of skincare products is considered to be affected during the pandemic period; however, this has not been investigated yet. Objective To determine whether the ongoing pandemic has changed people's habits related to skincare products and cosmetic procedures. Methods We conducted a multicenter survey study covering both private and public hospitals. Sociodemographic characteristics and clinical diagnoses of the patients were noted by dermatologists. A survey was used to determine the type and application areas of skincare products and cosmetic procedures before and during the pandemic, whether the patients were concerned about being infected, and any change in the make-up habits of female patients. Results Of the 1437 patients, 86.7% presented to the hospital due to dermatological complaints and 13.3% for cosmetic procedures. The rate of those that did not use skincare products was 0.05% before and 0.06% during the pandemic. Of the participants, 43.2% had undergone cosmetic procedures before and 38.1% during the pandemic. For both periods, the most frequent cosmetic procedure applied was laser epilation, followed by skincare treatment and chemical peeling. While undergoing these procedures, 34.9% of the patients were concerned about contracting coronavirus. Conclusion There was no significant difference in the use of skincare products and cosmetic procedures before and during the pandemic. The majority of the patients continued to undergo these procedures despite their fear of being infected. This shows that during the pandemic period, patients are concerned with their appearance and continue to undergo cosmetic procedures and pay attention to their skincare