1,512 research outputs found
Rational design of a (S)-selective-transaminase for asymmetric synthesis of (1S)-1-(1,1âČ-biphenyl-2-yl)ethanamine
Amine transaminases offer an environmentally sustainable synthesis route for the production of pure chiral amines. However, their catalytic efficiency toward bulky ketone substrates is greatly limited by steric hindrance and therefore presents a great challenge for industrial synthetic applications. We hereby report an example of rational transaminase enzyme design to help alleviate these challenges. Starting from the Vibrio fluvialis amine transaminase that has no detectable catalytic activity toward the bulky aromatic ketone 2-acetylbiphenyl, we employed a rational design strategy combining in silico and in vitro studies to engineer the transaminase enzyme with a minimal number of mutations, achieving an high catalytic activity and high enantioselectivity. We found that, by introducing two mutations W57G/R415A, detectable enzyme activity was achieved. The rationally designed variant, W57F/R88H/V153S/K163F/I259M/R415A/V422A, showed an improvement in reaction rate by more than 1716-fold toward the bulky ketone under study, producing the corresponding enantiomeric pure (S)-amine (enantiomeric excess (ee) value of >99%)
CP violation in in the model III 2HDM
We have calculated the Wilson coefficients (i=1,2) in the
renormalization scheme in the model III 2HDM. Using the obtained
Wilson coefficients, we have analyzed the CP violation in decays (q=d,s) in the model. The CP asymmetry, , depends on the
parameters of models and in can be as large as 40% and
35% for and respectively. It can reach 4% for decays.
Because in SM CP violation is smaller than or equal to O() which is
unobservably small, an observation of CP asymmetry in the decays would unambiguously signal the existence of new physics.Comment: revtex4, 16 pages, 7 figure
Transition Form Factor up to within the Factorization Approach
In the paper, we apply the factorization approach to deal with the
transition form factor in the large recoil
regions. The B-meson wave functions and that include the
three-particle Fock states' contributions are adopted to give a consistent PQCD
analysis of the form factor up to . It has been found that
both the wave functions and can give sizable
contributions to the form factor and should be kept for a better understanding
of the meson decays. Then the contributions from different twist structures
of the kaon wavefunction are discussed, including the -breaking
effects. A sizable contribution from the twist-3 wave function is
found, whose model dependence is discussed by taking two group of parameters
that are determined by different distribution amplitude moments obtained in the
literature. It is also shown that and
, which are more
reasonable and consistent with the light-cone sum rule results in the large
recoil regions.Comment: 22 pages and 6 figure
Size and shape analysis of error-prone shape data
We consider the problem of comparing sizes and shapes of objects when landmark data are prone to measurement error. We show that naive implementation of ordinary Procrustes analysis that ignores measurement error can compromise inference. To account for measurement error, we propose the conditional score method for matching configurations, which guarantees consistent inference under mild model assumptions. The effects of measurement error on inference from naive Procrustes analysis and the performance of the proposed method are illustrated via simulation and application in three real data examples. Supplementary materials for this article are available online
Deconfinement Transition for Quarks on a Line
We examine the statistical mechanics of a 1-dimensional gas of both adjoint
and fundamental representation quarks which interact with each other through
1+1-dimensional U(N) gauge fields. Using large-N expansion we show that, when
the density of fundamental quarks is small, there is a first order phase
transition at a critical temperature and adjoint quark density which can be
interpreted as deconfinement. When the fundamental quark density is comparable
to the adjoint quark density, the phase transition becomes a third order one.
We formulate a way to distinguish the phases by considering the expectation
values of high winding number Polyakov loop operators.Comment: Reported problems with figures fixed; 38 pages, LaTeX, 5 figures,
epsfi
Phase transitions in geometrothermodynamics
Using the formalism of geometrothermodynamics, we investigate the geometric
properties of the equilibrium manifold for diverse thermodynamic systems.
Starting from Legendre invariant metrics of the phase manifold, we derive
thermodynamic metrics for the equilibrium manifold whose curvature becomes
singular at those points where phase transitions of first and second order
occur. We conclude that the thermodynamic curvature of the equilibrium
manifold, as defined in geometrothermodynamics, can be used as a measure of
thermodynamic interaction in diverse systems with two and three thermodynamic
degrees of freedom
Regularization Independent Analysis of the Origin of Two Loop Contributions to N=1 Super Yang-Mills Beta Function
We present a both ultraviolet and infrared regularization independent
analysis in a symmetry preserving framework for the N=1 Super Yang-Mills beta
function to two loop order. We show explicitly that off-shell infrared
divergences as well as the overall two loop ultraviolet divergence cancel out
whilst the beta function receives contributions of infrared modes.Comment: 7 pages, 2 figures, typos correcte
High-resolution x-ray study of the nematic - smectic-A and smectic-A - smectic-C transitions in 8barS5-aerosil gels
The effects of dispersed aerosil nanoparticles on two of the phase
transitions of the thermotropic liquid crystal material
4-n-pentylphenylthiol-4'-n-octyloxybenzoate 8barS5 have been studied using
high-resolution x-ray diffraction techniques. The aerosils hydrogen bond
together to form a gel which imposes a weak quenched disorder on the liquid
crystal. The smectic-A fluctuations are well characterized by a two-component
line shape representing thermal and random-field contributions. An elaboration
on this line shape is required to describe the fluctuations in the smectic-C
phase; specifically the effect of the tilt on the wave-vector dependence of the
thermal fluctuations must be explicitly taken into account. Both the magnitude
and the temperature dependence of the smectic-C tilt order parameter are
observed to be unaffected by the disorder. This may be a consequence of the
large bare smectic correlation length in the direction of modulation for this
transition. These results show that the understanding developed for the nematic
to smectic-A transition for octylcyanobiphenyl (8CB) and octyloxycyanobiphenyl
(8OCB) liquid crystals with quenched disorder can be extended to quite
different materials and transitions.Comment: 7 pages, 8 figure
The Free Quon Gas Suffers Gibbs' Paradox
We consider the Statistical Mechanics of systems of particles satisfying the
-commutation relations recently proposed by Greenberg and others. We show
that although the commutation relations approach Bose (resp.\ Fermi) relations
for (resp.\ ), the partition functions of free gases are
independent of in the range . The partition functions exhibit
Gibbs' Paradox in the same way as a classical gas without a correction factor
for the statistical weight of the -particle phase space, i.e.\ the
Statistical Mechanics does not describe a material for which entropy, free
energy, and particle number are extensive thermodynamical quantities.Comment: number-of-pages, LaTeX with REVTE
The Quark-Hadron Phase Transition, QCD Lattice Calculations and Inhomogeneous Big-Bang Nucleosynthesis
We review recent lattice QCD results for the surface tension at the finite
temperature quark-hadron phase transition and discuss their implications on the
possible scale of inhomogeneities. In the quenched approximation the average
distance between nucleating centers is smaller than the diffusion length of a
protron, so that inhomogeneities are washed out by the time nucleosynthesis
sets in. Consequently the baryon density fluctuations formed by a QCD phase
transition in the early universe cannot significantly affect standard big-bang
nucleosynthesis calculations and certainly cannot allow baryons to close the
universe. At present lattice results are inconclusive when dynamical fermions
are included.Comment: 8 pages, LaTe
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