Long-term exposure of mouse pancreatic islets to oleate or palmitate results in reduced glucose-induced somatostatin and oversecretion of glucagon

AIMS/HYPOTHESIS: Long-term exposure to NEFAs leads to inhibition of glucose-induced insulin secretion. We tested whether the release of somatostatin and glucagon, the two other major islet hormones, is also affected. METHODS: Mouse pancreatic islets were cultured for 72 h at 4.5 or 15 mmol/l glucose with or without 0.5 mmol/l oleate or palmitate. The release of glucagon and somatostatin during subsequent 1 h incubations at 1 or 20 mmol/l glucose as well as the islet content of the two hormones were determined. Lipid-induced changes in islet cell ultrastructure were assessed by electron microscopy. RESULTS: Culture at 15 mmol/l glucose increased islet glucagon content by approximately 50% relative to that observed following culture at 4.5 mmol/l glucose. Inclusion of oleate or palmitate reduced islet glucagon content by 25% (at 4.5 mmol/l glucose) to 50% (at 15 mmol/l glucose). Long-term exposure to the NEFA increased glucagon secretion at 1 mmol/l glucose by 50% (when islets had been cultured at 15 mmol/l glucose) to 100% (with 4.5 mmol/l glucose in the culture medium) and abolished the inhibitory effect of 20 mmol/l glucose on glucagon secretion. Somatostatin content was unaffected by glucose and lipids, but glucose-induced somatostatin secretion was reduced by approximately 50% following long-term exposure to either of the NEFA, regardless of whether the culture medium contained 4.5 or 15 mmol/l glucose. Ultrastructural evidence of lipid deposition was seen in <10% of non-beta cells but in >80% of the beta cells. CONCLUSIONS/INTERPRETATION: Long-term exposure to high glucose and/or NEFA affects the release of somatostatin and glucagon. The effects on glucagon secretion are very pronounced and in type 2 diabetes in vivo may aggravate the hyperglycaemic effects due to lack of insulin

EMBRACE@Nancay: An Ultra Wide Field of View Prototype for the SKA

A revolution in radio receiving technology is underway with the development of densely packed phased arrays for radio astronomy. This technology can provide an exceptionally large field of view, while at the same time sampling the sky with high angular resolution. Such an instrument, with a field of view of over 100 square degrees, is ideal for performing fast, all-sky, surveys, such as the "intensity mapping" experiment to measure the signature of Baryonic Acoustic Oscillations in the HI mass distribution at cosmological redshifts. The SKA, built with this technology, will be able to do a billion galaxy survey. I will present a very brief introduction to radio interferometry, as well as an overview of the Square Kilometre Array project. This will be followed by a description of the EMBRACE prototype and a discussion of results and future plans.Comment: to appear in proceedings of the INFIERI Summer School INtelligent Signal Processing for FrontIEr Research and Industry, Paris 201

Characterization of a dense aperture array for radio astronomy

EMBRACE@Nancay is a prototype instrument consisting of an array of 4608 densely packed antenna elements creating a fully sampled, unblocked aperture. This technology is proposed for the Square Kilometre Array and has the potential of providing an extremely large field of view making it the ideal survey instrument. We describe the system,calibration procedures, and results from the prototype.Comment: 17 pages, accepted for publication in A&

Discovery of Molecular Gas in the Outflow and Tidal Arms around M82

We present the first fully sampled map of 12CO (1-0) emission from M82 covering the entire galaxy. Our map contains a 12 x 15 kpc^2 area. We find that extraplanar CO emission, previously reported at short distances above the galactic plane, extends to heights of up to 6 kpc above the disk. Some of this emission is associated with tidal arms seen in HI, implying either that M82 contained substantial amounts of molecular gas in the outer disk, or that molecular gas formed after the tidal features. CO emission along the direction of the outflow extends to distances of 3 kpc above and below the disk. At this distance, the line is shifted in velocity about 100 km/s, and has the same sense as the galactic outflow from the central starburst. This implies that molecular gas may be entrained into the outflow.Comment: 4 pages, 6 figures. Uses emulateapj5. Accepted by ApJ Letter

The Star Clusters in the Irregular Galaxy NGC 4449

We examine the star clusters in the irregular galaxy NGC 4449. We use a near-infrared spectrum and broad-band images taken with the HST to place a limit of 8--15 Myrs on the age of the bright central ojbect in NGC 4449. Its luminosity and size suggest that it is comparable to young super star clusters. However, there is a peculiar nucleated-bar structure at the center of this star cluster, and we suggest that this structure is debris from the interaction that has produced the counter-rotating gas systems and extended gas streamers in the galaxy. From the images we identify 60 other candidate compact star clusters in NGC 4449. Fourteen of these could be background elliptical galaxies or old globular star clusters. Of the star clusters, three, in addition to the central object, are potentially super star clusters, and many others are comparable to the populous clusters found in the LMC. The star clusters span a large range in ages with no obvious peak in cluster formation that might be attributed to the interaction that the galaxy has experienced.Comment: To be published in PASP, Feb. 2001; also attainable from ftp.lowell.edu, cd pub/dah/n4449pape

ISOCAM observations of the L1551 star formation region

The results of a deep mid-IR ISOCAM survey of the L1551 dark molecular cloud are presented. The aim of this survey is a search for new YSO (Young Stellar Object) candidates, using two broad-band filters centred at 6.7 and 14.3 micron. Although two regions close to the centre of L1551 had to be avoided due to saturation problems, 96 sources were detected in total (76 sources at 6.7 micron and 44 sources at 14.3 micron). Using the 24 sources detected in both filters, 14 were found to have intrinsic mid-IR excess at 14.3 micron and were therefore classified as YSO candidates. Using additional observations in B, V, I, J, H and K obtained from the ground, most candidates detected at these wavelengths were confirmed to have mid-IR excess at 6.7 micron as well, and three additional YSO candidates were found. Prior to this survey only three YSOs were known in the observed region (avoiding L1551 IRS5/NE and HL/XZ Tau). This survey reveals 15 new YSO candidates, although several of these are uncertain due to their extended nature either in the mid-IR or in the optical/near-IR observations. Two of the sources with mid-IR excess are previously known YSOs, one is a brown dwarf MHO 5 and the other is the well known T Tauri star HH30, consisting of an outflow and an optically thick disk seen edge on.Comment: 14 Pages, 8 Figure

The detached dust shells of AQ And, U Ant, and TT Cyg

Detached circumstellar dust shells are detected around three carbon variables using Herschel-PACS. Two of them are already known on the basis of their thermal CO emission and two are visible as extensions in IRAS imaging data. By model fits to the new data sets, physical sizes, expansion timescales, dust temperatures, and more are deduced. A comparison with existing molecular CO material shows a high degree of correlation for TT Cyg and U Ant but a few distinct differences with other observables are also found.Comment: Letter accepted for publication on the A&A Herschel Special Issu

Body mass index, mini nutritional assessment, and their association with five-year mortality in very old people

Objectives: To investigate the prevalence of malnutrition and the association between Body Mass Index (BMI), Mini Nutritional Assessment (MNA) and five-year mortality in a representative population of very old (>85 years) people. Design: A prospective cohort study. Setting: A population-based study of very old people in northern Sweden and western Finland, living in institutional care or in the community. Participants: Out of 1195 potential participants, 832 were included (mean age 90.2[+ or -]4.6 years). Measurements: Nutritional status was assessed using BMI and MNA and the association of those two variables with five-year mortality was analyzed. Results: The mean BMI value for the whole population was 25.1[+ or -]4.5 kg/m2, with no difference between genders (P=0.938). The mean MNA score was 22.5[+ or -]4.6 for the whole sample, and it was lower for women than for men (PA<0.001). Thirteen percent were malnourished (MNA<17) and 40.3% at risk of malnutrition (MNA 17--23.5) according to MNA. Also, 34.8% of those with a MNA score A<17 still had a BMI value a[yen]22.2 kg/m2. A BMI value A<22.2 kg/m2 and a MNA scoreA<17 were associated with lower survival. The association with mortality seemed to be J-shaped for BMI, and linear for MNA. Conclusions: Malnutrition according to MNA was common, but a substantial portion of those with a low MNA score still had a high BMI value, and vice versa. The association with mortality appeared to be J-shaped for BMI, and linear for MNA. The MNA seems to be a good measurement of malnutrition in very old people, and BMI might be misleading and could underestimate the prevalence of malnutrition, especially in women

Three-component modeling of C-rich AGB star winds I. Method and first results

Radiative acceleration of newly-formed dust grains and transfer of momentum from the dust to the gas plays an important role for driving winds of AGB stars. Therefore a detailed description of the interaction of gas and dust is a prerequisite for realistic models of such winds. In this paper we present the method and first results of a three-component time-dependent model of dust-driven AGB star winds. With the model we plan to study the role and effects of the gas-dust interaction on the mass loss and wind formation. The wind model includes separate conservation laws for each of the three components of gas, dust and the radiation field and is developed from an existing model which assumes position coupling between the gas and the dust. As a new feature we introduce a separate equation of motion for the dust component in order to fully separate the dust phase from the gas phase. The transfer of mass, energy and momentum between the phases is treated by interaction terms. We also carry out a detailed study of the physical form and influence of the momentum transfer term (the drag force) and three approximations to it. In the present study we are interested mainly in the effect of the new treatment of the dust velocity on dust-induced instabilities in the wind. As we want to study the consequences of the additional freedom of the dust velocity on the model we calculate winds both with and without the separate dust equation of motion. The wind models are calculated for several sets of stellar parameters. We find that there is a higher threshold in the carbon/oxygen abundance ratio at which winds form in the new model. The winds of the new models, which include drift, differ from the previously stationary winds, and the winds with the lowest mass loss rates no longer form.Comment: 15 pages, 5 figures, accepted by A&

Unexpectedly large mass loss during the thermal pulse cycle of the red giant R Sculptoris!

The asymptotic giant branch star R Sculptoris is surrounded by a detached shell of dust and gas. The shell originates from a thermal pulse during which the star undergoes a brief period of increased mass loss. It has hitherto been impossible to constrain observationally the timescales and mass-loss properties during and after a thermal pulse - parameters that determine the lifetime on the asymptotic giant branch and the amount of elements returned by the star. Here we report observations of CO emission from the circumstellar envelope and shell around R Sculptoris with an angular resolution of 1.3 arcsec. What was hitherto thought to be only a thin, spherical shell with a clumpy structure, is revealed to contain a spiral structure. Spiral structures associated with circumstellar envelopes have been seen previously, from which it was concluded that the systems must be binaries. Using the data, combined with hydrodynamic simulations, we conclude that R Sculptoris is a binary system that underwent a thermal pulse approximately 1800 years ago, lasting approximately 200 years. About 0.003 Msun of mass was ejected at a velocity of 14.3 km s-1 and at a rate approximately 30 times higher than the prepulse mass-loss rate. This shows that approximately 3 times more mass is returned to the interstellar medium during and immediately after a pulse than previously thought.Comment: Accepted by Natur