85 research outputs found
Implementation of a curriculum with radical change: feelings of pleasure and suffering
Esta investigación ha tenido como objetivo
verificar si las enfermeras docentes viven sentimientos de
placer y sufrimiento generados en el trabajo a la
implementación de cambio curricular radical. Es focalizada
la comprensión acerca de la concepción que la enfermera
tiene del ejercicio de su función como docente en el
cotidiano de su práctica profesional. Este es un estudio
cuantitativo que utiliza como método la descripción
exploratoria, siendo usado como instrumento para recoger
los datos una escala del tipo Likert, compuesta de cuestiones
distribuidas en tres factores: valoración, desgaste y
reconocimiento, los cuales son indicadores de placer y
sufrimiento. Hicieron parte de esta investigación enfermeras
docentes del curso de graduación en Enfermería de la
Universidade Estadual de Londrina. Los resultados
demostraron que la mayoría de las enfermeras docentes tiene
más placer que sufrimiento, pues, frecuentemente, se sienten
desgastadas en sus actividades en la implementación del
cambio curricular radical. Los datos revelan cómo son
importantes las relaciones de los docentes con sus tareas y
sus colegas, pues en ese espacio se establecen los factores
que interaccionan directamente en los sentimientos de placer
y sufrimiento.The present research aimed to verify if
nursing teachers experience feelings of pleasure and
suffering in their work due to the implementation of a radical
curricular change. It focuses on the understanding of nurses conception about their teaching role in their daily
professional practice. Its a quantitative study, using the
exploratory descriptive method, and a Likert type scale
entailing (39) questions distributed in three factors: valueing,
stress and recognition, indicators of pleasure and suffering,
as the instrument. Thirty (30) nursing teachers of the Nursing
graduation course of The State University of Londrina
participated in this research. The results demonstrated that
most nursing teachers feel more pleasure than suffering,
because, they are often valued, sometimes, they are
recognized and sometimes, they do not get stressed by their
activities in the implementation of a radical curricular
change. Those data reveal how important the relationships
of the educational nurses with theirs tasks and colleagues
are, once it is in this setting that the factors that directly
interact in the feelings of pleasure and suffering are brought
on.Apresente pesquisa teve como objetivo verificar
se as enfermeiras docentes vivenciam sentimentos de prazer
e de sofrimento gerados no trabalho, frente à implementação
de uma mudança curricular radical. Focaliza a compreensão
a respeito da concepção que a enfermeira tem do exercício
de sua função como docente no cotidiano de sua prática
profissional. Trata-se de um estudo quantitativo, usando
como método descrição exploratória, sendo que o
instrumento utilizado para a coleta de dados foi uma escala
tipo Likert composta de 39 questões distribuídas em três
fatores: valorização, desgaste e reconhecimento que são
indicadores de prazer e sofrimento. Fizeram parte desta
pesquisa 30 enfermeiras docentes do curso de graduação
em Enfermagem da Universidade Estadual de Londrina. Os
resultados demonstraram que a maioria das enfermeiras
docentes tem mais prazer do que sofrimento, pois,
freqüentemente, sentem-se valorizadas, às vezes, sentemse
reconhecidas e, às vezes, não se sentem desgastadas em
suas atividades na implementação da mudança curricular
radical. Esses dados nos revelam o quanto é importante as
relações dos docentes com suas tarefas e com suas colegas,
por ser neste espaço que se estabelecem os fatores que
interagem diretamente nos sentimentos de prazer e
sofrimento
Motor uncoordination and neuropathology in a transgenic mouse model of Machado-Joseph disease lacking intranuclear inclusions and ataxin-3 cleavage products
Machado-Joseph disease (MJD) is a late-onset neurodegenerative disorder caused by a polyglutamine (polyQ) expansion in the ataxin-3 protein. We generated two transgenic mouse lineages expressing the expanded human ataxin-3 under the control of the CMV promoter: CMVMJD83 and CMVMJD94, carrying Q83 and Q94 stretches, respectively. Behavioral analysis revealed that the CMVMJD94 transgenic mice developed motor uncoordination, intergenerational instability of the CAG repeat and a tissue-specific increase in the somatic mosaicism of the repeat with aging. Histopathological analysis of MJD mice at early and late stages of the disease revealed neuronal atrophy and astrogliosis in several brain regions; however, we found no signs of microglial activation or neuroinflammatory response prior to the appearance of an overt phenotype. In our model, the appearance of MJD-like symptoms was also not associated with the presence of ataxin-3 cleavage products or intranuclear aggregates. We propose the transgenic CMVMJD94 mice as a useful model to study the early stages in the pathogenesis of MJD and to explore the molecular mechanisms involved in CAG repeat instability.We would like to thank to Dr. Henry Paulson for providing the anti-ataxin-3 serum, Dr. Monica Sousa for the pCMV vector and to Eng. Lucilia Goreti Pinto for technical assistance. AS-F., M.C.C., S.S. and C.B. received FCT fellowships (SFRH/BD/15910/2005; SFRH/BPD/28560/2006; PTDC/SAU-GMG/64076/2006; SFRH/BPD/20987/2004). This research was funded by Fundacao para a Ciencia e Tecnologia through projects FEDER/FCT, POCI/SAU-MMO/60412/2004, PTDC/SAU-GMG/64076/2006; and Ataxia MJD Research Project
Association of 3-Phenoxybenzoic Acid Exposure during Pregnancy with Maternal Outcomes and Newborn Anthropometric Measures: Results from the IoMum Cohort Study
The aims of this study were to characterize the exposure of pregnant women living in
Portugal to 3-phenoxybenzoic acid (3-PBA) and to evaluate the association of this exposure with
maternal outcomes and newborn anthropometric measures. We also aimed to compare exposure
in summer with exposure in winter. Pregnant women attending ultrasound scans from April 2018
to April 2019 at a central hospital in Porto, Portugal, were invited to participate. Inclusion criteria
were: gestational week between 10 and 13, confirmed fetal vitality, and a signature of informed
consent. 3-PBA was measured in spot urine samples by gas chromatography with mass spectrometry
(GC-MS). The median 3-PBA concentration was 0.263 (0.167; 0.458) μg/g creatinine (n = 145). 3-PBA
excretion was negatively associated with maternal pre-pregnancy body mass index (BMI) (p = 0.049),
and it was higher during the summer when compared to winter (p < 0.001). The frequency of fish or
yogurt consumption was associated positively with 3-PBA excretion, particularly during the winter
(p = 0.002 and p = 0.015, respectively), when environmental exposure is low. Moreover, 3-PBA was
associated with levothyroxine use (p = 0.01), a proxy for hypothyroidism, which could be due to a
putative 3-PBA—thyroid hormone antagonistic effect. 3-PBA levels were not associated with the
anthropometric measures of the newborn. In conclusion, pregnant women living in Portugal are
exposed to 3-PBA, particularly during summer, and this exposure may be associated with maternal
clinical features.This article was supported by national funds through the FCT Foundation for Science and Technology, I.P., within the scope of the projects RISE - LA/P/0053/2020; CINTESIS, R&D UNIT (reference UIDB/4255/2020) and LAQV (references: UIDB/50006/2020 AND UIDP/50006/2020). Virgínia Cruz Fernandes was funded by FCT/MCTES (Foundation for Science and Technology and Ministry of Science, Technology and Higher Education) and the ESF (European Social Fund) through NORTE 2020 (North Region Operational Program) through a grant of Post-Doc (reference SFRH/BPD/109153/2015). Juliana Guimarães was funded by FCT/MCTES (Foundation for Science and Technology and Ministry of Science, Technology and Higher Education) under CINTESIS by a PhD scholarship (reference UI/BD/152087/2021).
The authors would like to thank the kind participation of all pregnant women and the support of the recruitment activities by the health professionals of the Department of Obstetrics, Centro Hospitalar Universitário S. João, Porto, Portugal and also to the professionals of REQUIMTE/LAQV, Instituto Superior de Engenharia, Politécnico do Porto, for their collaboration in carrying out the analyzes of the 3-PBA metabolite. This work received support from PT national funds (FCT/MCTES, Fundação para a Ciência e Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior) through the projects LA/P/0053/2020; UIDB/4255/2020; UIDB/5006/2020 and UIDP/50006/2020. Virgínia Cruz Fernandes thanks FCT/MCTES (Fundação para a Ciência e Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior) and ESF (European Social Fund) through NORTE 2020 (Programa Operacional Região Norte) for his/her Post-Doc grant ref. SFRH/BPD/109153/2015). Juliana Guimarães was funded by FCT/MCTES (Foundation for Science and Technology and Ministry of Science, Technology and Higher Education) under CINTESIS by a PhD scholarship (reference UI/BD/152087/2021).info:eu-repo/semantics/publishedVersio
Enzyme replacement therapy with galsulfase in 34 children younger than five years of age with MPS VI
Background: Mucopolysaccharidosis type VI (MPS VI) is a progressive, chronic and multisystem lysosomal storage disease with a wide disease spectrum. Clinical and biochemical improvements have been reported for MPS VI patients on enzyme replacement therapy (ERT) with rhASB (recombinant human arylsulfatase B; galsulfase, Naglazyme (R), BioMarin Pharmaceutical Inc.), making early diagnosis and intervention imperative for optimal patient outcomes. Few studies have included children younger than five years of age. This report describes 34 MPS VI patients that started treatment with galsulfase before five years of age.Methods: Data from patients who initiated treatment at <5 years of age were collected from patients' medical records. Baseline and follow-up assessments of common symptoms that led to diagnosis and that were used to evaluate disease progression and treatment efficacy were evaluated.Results: A significant negative correlation was seen with treatment with ERT and urinary GAG levels. of those with baseline and follow-up growth data, 47% remained on their pre-treatment growth curve or moved to a higher percentile after treatment. of the 9 patients with baseline and follow-up sleep studies, 5 remained unaffected and 1 patient initially with mild sleep apnea showed improvement. Data regarding cardiac, ophthalmic, central nervous system, hearing, surgical interventions and development are also reported. No patient discontinued treatment due to an adverse event and all that were treatment-emergent resolved.Conclusions: the prescribed dosage of 1 mg/kg IV weekly with galsulfase ERT is shown to be safe and effective in slowing and/or improving certain aspects of the disease, although patients should be closely monitored for complications associated with the natural history of the disease, especially cardiac valve involvement and spinal cord compression. A long-term follow-up investigation of this group of children will provide further information on the benefits of early treatment as well as disease progression and treatment efficacy and safety in this young patient population. (C) 2013 Elsevier Inc. All rights reserved.BioMarin Pharmaceutical Inc.ShireGenzymeBioMarinFiocruz MS, Inst Nacl Saude Mulher Crianca & Adolescente Fern, Ctr Genet Med, BR-22250020 Rio de Janeiro, RJ, BrazilUniv Fed Bahia, Serv Genet Med, Salvador, BA, BrazilHosp Albert Sabin, Fortaleza, Ceara, BrazilUniv Fed Mato Grosso do Sul, Fac Med, Campo Grande, MS USAUniv São Paulo, Inst Crianca, São Paulo, BrazilHosp Barao de Lucena, Recife, PE, BrazilUniv Fed Parana, Hosp Clin, BR-80060000 Curitiba, Parana, BrazilCtr Reabilitacao Infantil, Natal, RN, BrazilHosp Univ Maranhao, Sao Luis, MA, BrazilUniversidade Federal de São Paulo, Ctr Referencia Erros Inatos Metab, São Paulo, SP, BrazilHosp São Paulo, Enzyme Replacement Therapy Serv, Hosp & Maternidade Celso Pierro, São Paulo, BrazilUniv Fed Rio Grande do Norte, HOSPED, Hosp Pediat Prof Heriberto Ferreira Bezerra, Natal, RN, BrazilUniv Fortaleza, Fortaleza, Ceara, BrazilUniv Fed Rio Grande do Norte, BR-59072970 Natal, RN, BrazilUniv Fed Triangulo Mineiro, Uberaba, MG, BrazilHosp Clin Acre, Rio Branco, AC, BrazilUniv Fed Espirito Santo, HUCAM, Vitoria, ES, BrazilUniversidade Federal de São Paulo, Ctr Referencia Erros Inatos Metab, São Paulo, SP, BrazilHosp São Paulo, Enzyme Replacement Therapy Serv, Hosp & Maternidade Celso Pierro, São Paulo, BrazilWeb of Scienc
Determinants of the exclusive breastfeeding abandonment: psychosocial factors
OBJECTIVE To assess the determinants of exclusive breastfeeding abandonment. METHODS Longitudinal study based on a birth cohort in Viçosa, MG, Southeastern Brazil. In 2011/2012, 168 new mothers accessing the public health network were followed. Three interviews, at 30, 60, and 120 days postpartum, with the new mothers were conducted. Exclusive breastfeeding abandonment was analyzed in the first, second, and fourth months after childbirth. The Edinburgh Postnatal Depression Scale was applied to identify depressive symptoms in the first and second meetings, with a score of ≥ 12 considered as the cutoff point. Socioeconomic, demographic, and obstetric variables were investigated, along with emotional conditions and the new mothers’ social network during pregnancy and the postpartum period. RESULTS The prevalence of exclusive breastfeeding abandonment at 30, 60, and 120 days postpartum was 53.6% (n = 90), 47.6% (n = 80), and 69.6% (n = 117), respectively, and its incidence in the fourth month compared with the first was 48.7%. Depressive symptoms and traumatic delivery were associated with exclusive breastfeeding abandonment in the second month after childbirth. In the fourth month, the following variables were significant: lower maternal education levels, lack of homeownership, returning to work, not receiving guidance on breastfeeding in the postpartum period, mother’s negative reaction to the news of pregnancy, and not receiving assistance from their partners for infant care. CONCLUSIONS Psychosocial and sociodemographic factors were strong predictors of early exclusive breastfeeding abandonment. Therefore, it is necessary to identify and provide early treatment to nursing mothers with depressive symptoms, decreasing the associated morbidity and promoting greater duration of exclusive breastfeeding. Support from health professionals, as well as that received at home and at work, can assist in this process
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