Coccidiose hos kvæg: En oversigt over coccidiearter, patogenese, epidemiologi og forebyggelse specielt i økologiske besætninger

Coccidiose hos kalve forårsages af arter af de encellede parasitter, Eimeria, der findes vidt udbredt i alle verdensdele, og som giver anledning til diarré og utrivelighed hos især unge og modtagelige dyr. Hos kvæg findes 12 forskellige Eimeria arter, der adskiller sig fra hinanden med hensyn til morfologi, livscyklus og patogenitet. Infektion med Eimeria kan diagnosticeres ved forskellige la-boratoriemetoder, hvorved bl.a. antallet og arten af coccidier forsøges fastslået. Således skabes grundlag for bestemmelse af hvilke forebyggende tiltag, der eventuelt bør iværksættes. Ved under-søgelse af 258 kalve fra 25 danske økologiske malkekvægsbesætninger fandt vi Eimeria-arter i alle (100%) besætninger og i 88% af kalvene. De fleste kalve udskilte meget lavgradige mængder af coccidier i gødningen, medens nogle havde særdeles høje coccidieudskillelser. Coccidiose er en multifaktoriel sygdom, og sværhedsgraderne af sygdommen varierer derfor fra subkliniske tilfælde til nogle med løs, pastøs fæces af en enkelt dags varighed eller profus diarré med dysenteri, tenesmus og evt. mors. De kliniske symptomer tillægges traditionelt tilstedeværelsen af coccidiearterne Eimeria zuernii og E. bovis. Kalvens modtagelighed for coccidiose afhænger bl.a. af råmælksforsyning og immunitet, ernæringsstatus, stress, og evt. forekomst af andre sygdomme (f.eks. samtidige helminth- eller virusinfektioner). Endelig har også miljøet betydning for udvikling af coccidiose, herunder opstaldningstæthed, klima og hygiejne. Total udryddelse af coccidier er ikke muligt pga. parasittens ubikvitære forekomst og resistens i miljøet, men kalven kan styrkes ved sikring af tilstrækkelige råmælksmængder og ved opretholdelse af høj hygiejne i kælvningsboksen, samt efterfølgende opstaldningsforhold. Aktiv immunisering (vaccination) har endnu ikke vist sig effektiv i større målestok. Der foreligger ikke yderligere retningslinier for forebyggelse i perspektiv af de økologiske regler. Kalve, der har klinisk coccidiose, bør isoleres fra de øvrige kalve og gives væsketerapi for at opretholde væskebalancen. Den bedste behandling er imidlertid forebyggelse - med fokus på belægningsgrad og høj hygiejne inklusive rigeligt med tør strøelse

Sundhed og velfærd i kalveholdet

Gennem de seneste år har vi arbejdet med en forståelse af dyrevelfærd i økologiske besætninger, hvor begrebet “naturlighed” har fået stor betydning. I en malkekvægbesætning er det ikke muligt at tilbyde køerne 100% naturlige forhold, men man kan tilstræbe at dyrene får mulighed for at udføre deres naturlige adfærd, har en stor grad af valgfrihed, har frisk luft, dagslys og adgang til udearealer. Det stiller imidlertid større krav til de mennesker, som arbejder med dyrene, bl.a. i forhold til øget opsyn samt til bedre at kunne vurdere, hvordan dyrene har det. I projektet er der bl.a. blevet udviklet en model til vurdering af velfærd hos kalve. Den første version er blevet implementeret i rådgivning af økologiske besætninger. Den forventes endvidere afprøvet i Norge, Storbritannien og Holland

genetic structuring and differentiation of echinococcus multilocularis in slovakia assessed by sequencing and isoenzyme studies

AbstractNucleotide sequencing of the mitochondrial cytochrome c oxidase subunit 1 (CO1) gene and isoenzyme analysis were used to survey the genetic variability in Echinococcus multilocularis populations from Slovakia. A sample of 12 isolates acquired from 10 different districts from red foxes exhibited identical sequences. Compared with the previously described E. multilocularis variants, one base substitution was consistently observed relative to the M1 variant (detected in China, Alaska, North America, Japan) and three base substitutions were recorded relative to the M2 variant (detected in Germany) in the CO1 fragment. These data, along with the recently gathered data from French isolates, are indicative of a genetically unique population occurring in Central and Western Europe. Electrophoretic examination of enzymes produced by 14 gene loci revealed intraspecific polymorphism only with the glucose-phosphate isomerase (two distinct patterns) and the mannosephosphate isomerase (four genotypes composed of three alleles) enzyme systems. To allow a fast species differentiation of E. multilocularis and E. granulosus (specifically, the G7 genotype occurring in Slovakia), discriminative electrophoretic characters between the species were obtained by isoenzyme analysis. Fixed genetic differences between the species were detected in the glucose-phosphate isomerase, esterase and aldolase systems, and partial differences were detected in four additional systems

Advances in prevention and therapy of neonatal dairy calf diarrhoea : a systematical review with emphasis on colostrum management and fluid therapy

Neonatal calf diarrhoea remains the most common cause of morbidity and mortality in preweaned dairy calves worldwide. This complex disease can be triggered by both infectious and non-infectious causes. The four most important enteropathogens leading to neonatal dairy calf diarrhoea are Escherichia coli, rota-and coronavirus, and Cryptosporidium parvum. Besides treating diarrhoeic neonatal dairy calves, the veterinarian is the most obvious person to advise the dairy farmer on prevention and treatment of this disease. This review deals with prevention and treatment of neonatal dairy calf diarrhoea focusing on the importance of a good colostrum management and a correct fluid therapy

Characterization of bovine embryos cultured under conditions appropriate for sustaining human naïve pluripotency.

In mammalian preimplantation development, pluripotent cells are set aside from cells that contribute to extra-embryonic tissues. Although the pluripotent cell population of mouse and human embryos can be cultured as embryonic stem cells, little is known about the pathways involved in formation of a bovine pluripotent cell population, nor how to maintain these cells in vitro. The objective of this study was to determine the transcriptomic profile related to bovine pluripotency. Therefore, in vitro derived embryos were cultured in various culture media that recently have been reported capable of maintaining the naïve pluripotent state of human embryonic cells. Gene expression profiles of embryos cultured in these media were compared using microarray analysis and quantitative RT-PCR. Compared to standard culture conditions, embryo culture in 'naïve' media reduced mRNA expression levels of the key pluripotency markers NANOG and POU5F1. A relatively high percentage of genes with differential expression levels were located on the X-chromosome. In addition, reduced XIST expression was detected in embryos cultured in naïve media and female embryos contained fewer cells with H3K27me3 foci, indicating a delay in X-chromosome inactivation. Whole embryos cultured in one of the media, 5iLA, could be maintained until 23 days post fertilization. Together these data indicate that 'naïve' conditions do not lead to altered expression of known genes involved in pluripotency. Interestingly, X-chromosome inactivation and development of bovine embryos were dependent on the culture conditions

Fatores de risco associados à infecção por Cryptosporidium spp. e Giardia duodenalis em bovinos leiteiros na fase de cria e recria na mesorregião do Campo das Vertentes de Minas Gerais

Este estudo observacional do tipo transversal foi realizado com o objetivo avaliar os fatores de risco associados à infecção por Cryptosporidium spp. e Giardia duodenalis em bezerras provenientes de 20 propriedades leiteiras, localizadas na mesorregião do Campo das Vertentes de Minas Gerais. As propriedades foram divididas igualmente em dois grupos de acordo com o tipo de leite produzido: Grupo I = Leite B e Grupo II = Leite cru refrigerado. Amostras fecais de 356 bezerras foram coletadas no período de setembro de 2008 a agosto de 2009 e analisadas utilizando-se os métodos de Ziehl-Neelsen e flutuação em sulfato zinco a 33% para detecção, respectivamente, dos oocistos de Cryptosporidium spp. e cistos de G. duodenalis. Dados sobre práticas de manejo e condições sanitárias de criação dos bovinos foram obtidos por meio de entrevistas durante a visita a cada propriedade, no momento em que foi coletada uma única amostra de fezes de bezerras de 1 dia a 12 meses de idade. A frequência média global de bezerras infectadas por Cryptosporidium spp. foi de 21,62%, sendo a faixa etária de 7- 21 dias de idade a que apresentou o maior número de animais eliminando oocistos. Para G. duodenalis, a frequência média global foi de 25,56% e a faixa etária de 60-90 dias de idade foi a com maior número de animais com cistos nas fezes. Os resultados deste estudo indicam que infecções por Cryptosporidium spp. e G. duodenalis estão amplamente distribuídas entre fêmeas bovinas na fase de cria e recria provenientes de rebanhos leiteiros na mesorregião do Campo das Vertentes de Minas Gerais. Dentre os fatores associados a um maior risco de infecção por Cryptosporidium spp. e G. duodenalis em bezerras, discutidos neste estudo, se destacam os seguintes: a permanência no piquete maternidade por mais de 12h após o nascimento; o fornecimento de colostro a partir de 7h de vida; o primeiro fornecimento de água e concentrado entre 1 e 7 dias de idade; e a manutenção em instalação coletiva e/ou localizada próxima ao curral

Uncoupled Embryonic and Extra-Embryonic Tissues Compromise Blastocyst Development after Somatic Cell Nuclear Transfer

Somatic cell nuclear transfer (SCNT) is the most efficient cell reprogramming technique available, especially when working with bovine species. Although SCNT blastocysts performed equally well or better than controls in the weeks following embryo transfer at Day 7, elongation and gastrulation defects were observed prior to implantation. To understand the developmental implications of embryonic/extra-embryonic interactions, the morphological and molecular features of elongating and gastrulating tissues were analysed. At Day 18, 30 SCNT conceptuses were compared to 20 controls (AI and IVP: 10 conceptuses each); one-half of the SCNT conceptuses appeared normal while the other half showed signs of atypical elongation and gastrulation. SCNT was also associated with a high incidence of discordance in embryonic and extra-embryonic patterns, as evidenced by morphological and molecular “uncoupling”. Elongation appeared to be secondarily affected; only 3 of 30 conceptuses had abnormally elongated shapes and there were very few differences in gene expression when they were compared to the controls. However, some of these differences could be linked to defects in microvilli formation or extracellular matrix composition and could thus impact extra-embryonic functions. In contrast to elongation, gastrulation stages included embryonic defects that likely affected the hypoblast, the epiblast, or the early stages of their differentiation. When taking into account SCNT conceptus somatic origin, i.e. the reprogramming efficiency of each bovine ear fibroblast (Low: 0029, Med: 7711, High: 5538), we found that embryonic abnormalities or severe embryonic/extra-embryonic uncoupling were more tightly correlated to embryo loss at implantation than were elongation defects. Alternatively, extra-embryonic differences between SCNT and control conceptuses at Day 18 were related to molecular plasticity (high efficiency/high plasticity) and subsequent pregnancy loss. Finally, because it alters re-differentiation processes in vivo, SCNT reprogramming highlights temporally and spatially restricted interactions among cells and tissues in a unique way