1,180 research outputs found
Genetic analysis of the Linnaean Ulva lactuca (Ulvales, Chlorophyta) holotype and related type specimens reveals name misapplications, unexpected origins, and new synonymies.
Current usage of the name Ulva lactuca, the generitype of Ulva, remains uncertain. Genetic analyses were performed on the U. lactuca Linnaean holotype, the U. fasciata epitype, the U. fenestrata holotype, the U. lobata lectotype, and the U. stipitata lectotype. The U. lactuca holotype is nearly identical in rbcL sequence to the U. fasciata epitype, a warm temperate to tropical species, rather than the cold temperate species to which the name U. lactuca has generally been applied. We hypothesize that the holotype specimen of U. lactuca came from the Indo-Pacific rather than northern Europe. Our analyses indicate that U. fasciata and U. lobata are heterotypic synonyms of U. lactuca. Ulva fenestrata is the earliest name for northern hemisphere, cold temperate Atlantic and Pacific species, with U. stipitata a junior synonym. DNA sequences from type specimens provide an unequivocal method for applying names to Ulva species. This article is protected by copyright. All rights reserved
Elevated liver glycogenolysis mediates higher blood glucose during acute exercise in Barth syndrome
UNLABELLED: Barth syndrome (BTHS) is an X-linked recessive genetic disorder due to mutations in the Tafazzin (TAFAZZIN) gene that lead to cardiac and skeletal muscle mitochondrial dysfunction. Previous studies in humans with BTHS demonstrate that the defects in muscle mitochondrial oxidative metabolism result in an enhanced reliance on anaerobic metabolism during exercise to meet energy demands of muscular work. During exercise, the liver normally increases glucose production via glycogenolysis and gluconeogenesis to match the elevated rate of muscle glucose uptake and meet the ATP requirements of working muscle. However, the impact of Tafazzin deficiency on hepatic glucose production and the pathways contributing to hepatic glucose production during exercise is unknown. Therefore, the purpose of this study was to quantify in vivo liver gluconeogenesis and glycogenolysis in Tafazzin knockdown mice at rest and during acute exercise.
METHODS: Male TAFAZZIN shRNA transgenic (TG) and wild-type (WT) mice completed exhaustive treadmill running protocols to test exercise tolerance. Mice underwent 2H- and 13C-stable isotope infusions at rest and during a 30-minute treadmill running bout to quantify hepatic glucose production and associated nutrient fluxes under sedentary conditions and during acute exercise. Circulating and tissue (skeletal muscle and liver) samples were obtained during and following exercise to assess static metabolite levels.
RESULTS: TG mice reached exhaustion sooner during exhaustive treadmill running protocols and exhibited higher plasma lactate concentrations after exhaustive exercise compared to WT mice. Arterial glucose levels were comparable between genotypes at rest, but higher in TG mice compared to WT mice during exercise. Consistent with the higher blood glucose, TG mice showed increased endogenous glucose production owing to elevated glycogenolysis compared to WT mice during exercise. Total gluconeogenesis, gluconeogenesis from glycerol, gluconeogenesis from phosphoenolpyruvate, pyruvate cycling, total cataplerosis, and anaplerotic fluxes were similar between TG and WT mice at rest and during exercise. However, lactate dehydrogenase flux and TCA cycle fluxes trended higher in TG mice during exercise. Liver glycogen content in TG was higher in TG vs. controls.
CONCLUSION: Our data in the Tafazzin knockdown mouse suggest that elevated anaerobic metabolism during rest and exercise previously reported in humans with BTHS are supported by the finding of higher hepatic glycogenolysis
More than skin deep: Functional genomic basis for resistance to Amphibian Chytridiomycosis
The amphibian-killing chytrid fungus Batrachochytriumdendrobatidis (Bd) is one of themost generalist pathogens known, capable of
infecting hundreds of species globally and causing widespread population declines and extinctions. However, some host species are
seemingly unaffected by Bd, tolerating or clearing infections without clinical signs of disease. Variation in host immune responses is
commonly evoked for these resistant or tolerant species, yet to date,we have nodirect comparisonof amphibian species responses to
infection at the level of gene expression. In this study,we challenged four CentralAmerican frog species that vary in Bd susceptibility,
with a sympatric virulent strain of the pathogen. We compared skin and spleen orthologous gene expression using differential
expression tests and coexpression gene network analyses.Wefound that resistant species have reduced skin inflammatory responses
andincreased expressionofgenes involved inskin integrity. Incontrast, onlyhighly susceptible species exhibited suppressionof splenic
T-cell genes. We conclude that resistance to chytridiomycosis may be related to a species’ ability to escape the immunosuppressive
activity of the fungus. Moreover, our results indicate that within-species differences in splenic proteolytic enzyme gene expression
may contribute to intraspecific variation in survival. This first comparison of amphibian functional immunogenomic architecture in
response to Bd provides insights into key genetic mechanisms underlying variation in disease outcomes among amphibian species
Transfer/Breakup Modes in the 6He+209Bi Reaction Near and Below the Coulomb Barrier
Reaction products from the interaction of 6He with 209Bi have been measured
at energies near the Coulomb barrier. A 4He group of remarkable intensity,
which dominates the total reaction cross section, has been observed. The
angular distribution of the group suggests that it results primarily from a
direct nuclear process. It is likely that this transfer/breakup channel is the
doorway state that accounts for the previously observed large sub-barrier
fusion enhancement in this system.Comment: 4 pages; 3 figure
Aquilegia, Vol. 20 No. 3, July-September 1996: Newsletter of the Colorado Native Plant Society
https://epublications.regis.edu/aquilegia/1080/thumbnail.jp
Limits on diffuse fluxes of high energy extraterrestrial neutrinos with the AMANDA-B10 detector
Data from the AMANDA-B10 detector taken during the austral winter of 1997
have been searched for a diffuse flux of high energy extraterrestrial
muon-neutrinos, as predicted from, e.g., the sum of all active galaxies in the
universe. This search yielded no excess events above those expected from the
background atmospheric neutrinos, leading to upper limits on the
extraterrestrial neutrino flux. For an assumed E^-2 spectrum, a 90% classical
confidence level upper limit has been placed at a level E^2 Phi(E) = 8.4 x
10^-7 GeV cm^-2 s^-1 sr^-1 (for a predominant neutrino energy range 6-1000 TeV)
which is the most restrictive bound placed by any neutrino detector. When
specific predicted spectral forms are considered, it is found that some are
excluded.Comment: Submitted to Physical Review Letter
Detection of Atmospheric Muon Neutrinos with the IceCube 9-String Detector
The IceCube neutrino detector is a cubic kilometer TeV to PeV neutrino
detector under construction at the geographic South Pole. The dominant
population of neutrinos detected in IceCube is due to meson decay in cosmic-ray
air showers. These atmospheric neutrinos are relatively well-understood and
serve as a calibration and verification tool for the new detector. In 2006, the
detector was approximately 10% completed, and we report on data acquired from
the detector in this configuration. We observe an atmospheric neutrino signal
consistent with expectations, demonstrating that the IceCube detector is
capable of identifying neutrino events. In the first 137.4 days of livetime,
234 neutrino candidates were selected with an expectation of 211 +/-
76.1(syst.) +/- 14.5(stat.) events from atmospheric neutrinos
First LIGO search for gravitational wave bursts from cosmic (super)strings
We report on a matched-filter search for gravitational wave bursts from
cosmic string cusps using LIGO data from the fourth science run (S4) which took
place in February and March 2005. No gravitational waves were detected in 14.9
days of data from times when all three LIGO detectors were operating. We
interpret the result in terms of a frequentist upper limit on the rate of
gravitational wave bursts and use the limits on the rate to constrain the
parameter space (string tension, reconnection probability, and loop sizes) of
cosmic string models.Comment: 11 pages, 3 figures. Replaced with version submitted to PR
Limits on the high-energy gamma and neutrino fluxes from the SGR 1806-20 giant flare of December 27th, 2004 with the AMANDA-II detector
On December 27th 2004, a giant gamma flare from the Soft Gamma-ray Repeater
1806-20 saturated many satellite gamma-ray detectors. This event was by more
than two orders of magnitude the brightest cosmic transient ever observed. If
the gamma emission extends up to TeV energies with a hard power law energy
spectrum, photo-produced muons could be observed in surface and underground
arrays. Moreover, high-energy neutrinos could have been produced during the SGR
giant flare if there were substantial baryonic outflow from the magnetar. These
high-energy neutrinos would have also produced muons in an underground array.
AMANDA-II was used to search for downgoing muons indicative of high-energy
gammas and/or neutrinos. The data revealed no significant signal. The upper
limit on the gamma flux at 90% CL is dN/dE < 0.05 (0.5) TeV^-1 m^-2 s^-1 for
gamma=-1.47 (-2). Similarly, we set limits on the normalization constant of the
high-energy neutrino emission of 0.4 (6.1) TeV^-1 m^-2 s^-1 for gamma=-1.47
(-2).Comment: 14 pages, 3 figure
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