Characterization and winemaking application of a novel pectin-degrading enzyme complex from Aspergillus sojae ATCC 20235

A novel pectin-degrading enzyme complex produced by Aspergillus sojae ATCC 20235 (PC-AS) using lowcost substrates was characterised in terms of its enzyme activities relevant in winemaking. This novel PC-AS was applied at the maceration/fermentation stage during the elaboration of 'Tempranillo' red wines to study its effect on colour development and the phenolic and amino acid wine composition. PC-AS polygalacturonase activity was the major enzyme activity detected and quantified under winemaking conditions (pH 3.5, 20 °C) and proved being stable and active in the presence of sulfur dioxide. Xylanase activity, albeit in lesser amounts, was also present in PC-AS, and neither pectinesterase, which produces methanol, nor β-glucosidase, which is detrimental to wine colour, were detected in PC-AS. This pectin-degrading complex promoted a faster colour extraction since maximum colour intensity of the enzyme treated wines was reached earlier compared to their controls. After 6 months of storage under winery conditions, wines elaborated with PC-AS presented higher concentrations of caffeic acid, coumaric acid and aspartic acid (p ˂ 0.05), suggesting an improved extraction of grape cell components. In conclusion, the application of PCAS yielded results that showed that it can be used in red winemaking to shorten the maceration time needed to reach high CI values and to improve the extraction of some phenolics and other compounds that enhance the quality of the final product.Facultad de Ciencias ExactasCentro de Investigación y Desarrollo en Fermentaciones Industriale

Characterization and winemaking application of a novel pectin-degrading enzyme complex from Aspergillus sojae ATCC 20235

A novel pectin-degrading enzyme complex produced by Aspergillus sojae ATCC 20235 (PC-AS) using lowcost substrates was characterised in terms of its enzyme activities relevant in winemaking. This novel PC-AS was applied at the maceration/fermentation stage during the elaboration of 'Tempranillo' red wines to study its effect on colour development and the phenolic and amino acid wine composition. PC-AS polygalacturonase activity was the major enzyme activity detected and quantified under winemaking conditions (pH 3.5, 20 °C) and proved being stable and active in the presence of sulfur dioxide. Xylanase activity, albeit in lesser amounts, was also present in PC-AS, and neither pectinesterase, which produces methanol, nor β-glucosidase, which is detrimental to wine colour, were detected in PC-AS. This pectin-degrading complex promoted a faster colour extraction since maximum colour intensity of the enzyme treated wines was reached earlier compared to their controls. After 6 months of storage under winery conditions, wines elaborated with PC-AS presented higher concentrations of caffeic acid, coumaric acid and aspartic acid (p ˂ 0.05), suggesting an improved extraction of grape cell components. In conclusion, the application of PCAS yielded results that showed that it can be used in red winemaking to shorten the maceration time needed to reach high CI values and to improve the extraction of some phenolics and other compounds that enhance the quality of the final product.Facultad de Ciencias ExactasCentro de Investigación y Desarrollo en Fermentaciones Industriale

Sistema de predicción de incendios forestales basado en el índice FWI para la provincia de Córdoba

El objetivo de este proyecto es elaborar un modelo que permita pronosticar los incendios forestales en la Provincia de Córdoba mediante técnicas de aprendizaje automático (machine learning), utilizando para ello modelos supervisados como redes neuronales o maquinas de soporte vectorial. A partir del mismo, se pretende capturar la presencia de patrones de comportamiento de los factores que influyen en la producción de incendios forestales, ya sean humanos o de índole climática, tales como humedad, presión, temperatura y cantidad de lluvia caída en una zona determinada, asociados a incendios forestales.Eje: Agentes y Sistemas InteligentesRed de Universidades con Carreras en Informática (RedUNCI

Predicción de incendios forestales en la Provincia de Córdoba

El objetivo de este proyecto es el de elaborar un modelo que permita pronosticar los incendios forestales en la Provincia de Córdoba, especialmente en las sierras de Córdoba y la región del parque Chaqueño de la provincia. A partir del mismo, se pretende capturar la presencia de patrones de comportamiento de los factores que influyen en la producción de incendios forestales, ya sean humanos o de índole climática, tales como humedad, presión, temperatura y cantidad de lluvia caída en una zona determinada, asociados a incendios forestales. Este modelo ha sido elaborado empleando técnicas de aprendizaje automático (machine learning), utilizando para ello modelos supervisados como redes neuronales o maquinas vectores de soporte.Eje: Agentes y Sistemas InteligentesRed de Universidades con Carreras en Informática (RedUNCI

Design, Synthesis, Characterization, and Evaluation of the Anti-HT-29 Colorectal Cell Line Activity of Novel 8-Oxyquinolinate-Platinum(II)-Loaded Nanostructured Lipid Carriers Targeted with Riboflavin

Colorectal cancer is occasionally called colon or rectal cancer, depending on where cancer begins to form, and is the second leading cause of cancer death among both men and women. The platinum-based [PtCl(8-O-quinolinate)(dmso)] (8-QO-Pt) compound has demonstrated encouraging anticancer activity. Three different systems of 8-QO-Pt-encapsulated nanostructured lipid carriers (NLCs) with riboflavin (RFV) were investigated. NLCs of myristyl myristate were synthesized by ultrasonication in the presence of RFV. RFV-decorated nanoparticles displayed a spherical shape and a narrow size dispersion in the range of 144–175 nm mean particle diameter. The 8-QO-Pt-loaded formulations of NLC/RFV with more than 70% encapsulation efficiency showed sustained in vitro release for 24 h. Cytotoxicity, cell uptake, and apoptosis were evaluated in the HT-29 human colorectal adenocarcinoma cell line. The results revealed that 8-QO-Pt-loaded formulations of NLC/RFV showed higher cytotoxicity than the free 8-QO-Pt compound at 5.0 µM. All three systems exhibited different levels of cellular internalization. Moreover, the hemotoxicity assay showed the safety profile of the formulations (less than 3.7%). Taken together, RFV-targeted NLC systems for drug delivery have been investigated for the first time in our study and the results are promising for the future of chemotherapy in colon cancer treatmen

Lista anotada de los tipos de peces en la colección del Laboratorio de Ictiología, Universidad del Quindío, Armenia, Colombia (IUQ)

A comprehensive list of the types deposited in the collection of the Laboratory of Ichthyology of the University of Quindío, Armenia, Colombia (IUQ) was prepared. Type specimens for 63 species of freshwater bony fishes are deposited in IUQ, including 34 holotypes and 63 lots of paratypes. The specimens were verified and additional information is provided on their current condition, number, and locality information. A gallery of pictures of holotypes or paratypes of each nominal species is also presented.Se elaboró una lista completa de los tipos depositados en la colección del Laboratorio de Ictiología de laUniversidad del Quindío, Armenia, Colombia (IUQ). Ejemplares tipo de 63 especies nominales de peces óseoscontinentales han sido depositados en IUQ, incluye 34 holotipos y 63 lotes de paratipos. Se verificaron losejemplares e información adicional es provista sobre su condición actual, número e información de localidad.Una galería de fotografías de holotipos o paratipos de cada especie nominal es presentada

Lista anotada de los tipos de peces en la colección del Laboratorio de Ictiología, Universidad del Quindío, Armenia, Colombia (IUQ)

Se elaboró una lista completa de los tipos depositados en la colección del Laboratorio de Ictiología de la Universidad del Quindío, Armenia, Colombia (IUQ). Ejemplares tipo de 63 especies nominales de peces óseos continentales han sido depositados en IUQ, incluye 34 holotipos y 63 lotes de paratipos. Se verificaron los ejemplares e información adicional es provista sobre su condición actual, número e información de localidad. Una galería de fotografías de holotipos o paratipos de cada especie nominal es presentada

Characterization and winemaking application of a novel pectin-degrading enzyme complex from Aspergillus sojae ATCC 20235

A novel pectin-degrading enzyme complex produced by Aspergillus sojae ATCC 20235 (PC-AS) using lowcost substrates was characterised in terms of its enzyme activities relevant in winemaking. This novel PC-AS was applied at the maceration/fermentation stage during the elaboration of 'Tempranillo' red wines to study its effect on colour development and the phenolic and amino acid wine composition. PC-AS polygalacturonase activity was the major enzyme activity detected and quantified under winemaking conditions (pH 3.5, 20 °C) and proved being stable and active in the presence of sulfur dioxide. Xylanase activity, albeit in lesser amounts, was also present in PC-AS, and neither pectinesterase, which produces methanol, nor β-glucosidase, which is detrimental to wine colour, were detected in PC-AS. This pectin-degrading complex promoted a faster colour extraction since maximum colour intensity of the enzyme treated wines was reached earlier compared to their controls. After 6 months of storage under winery conditions, wines elaborated with PC-AS presented higher concentrations of caffeic acid, coumaric acid and aspartic acid (p ˂ 0.05), suggesting an improved extraction of grape cell components. In conclusion, the application of PCAS yielded results that showed that it can be used in red winemaking to shorten the maceration time needed to reach high CI values and to improve the extraction of some phenolics and other compounds that enhance the quality of the final product.Facultad de Ciencias ExactasCentro de Investigación y Desarrollo en Fermentaciones Industriale

Diseño cinemático de un robot paralelo 2-PRR

Este artículo presenta una síntesis dimensional para un robot plano paralelo 2-PRR con estructura simétrica. Este robot posibilita la translación de la mesa móvil sin cambios en la orientación, lo cual resulta útil en aplicaciones que requieren posiciones controladas con alta rigidez. Debido a la alta sensibilidad que tienen estos robots con respecto a los cambios geométricos, muchas metodologías han sido desarrolladas para establecer la síntesis dimensional. En este trabajo se usó el Método de Normalización de Parámetros Finitos (PFNM) para establecer las dimensiones usando dos atlas de diseño, para el Índice Globales de Condición (GCI), y para el espacio de trabajo ( ). Se muestra que, para los dos atlas de diseño, GCI y , no es posible maximizar uno de los índices sin disminuir el otro, lo que representa un compromiso de diseño. Igualmente, se resaltan configuraciones singulares que vienen de geometrías especificas o de posiciones limite. La síntesis dimensional completa del 2-PRR es también presentada.In civil construction Abstract— This paper presents a dimensional synthesis for a 2-PRR planar parallel robot with a structural plane of symmetry. This robot can achieve the translation of the moving platform without changing the orientation, being useful for applications that require controlled positions with high rigidity. Because the performance of parallel robots is highly sensitive to their geometric parameters, many methodologies to state the dimensional synthesis has been developed. We used the method of Parameter - Finiteness Normalization Method (PFNM) to state the dimensional synthesis using Global Condition Index (GCI) and workspace ( ) design atlases. For the two, GCI and , designed atlases, it is not possible to maximize one of the indexes without diminishing the other one, which represents a design compromise. Also, we remark singular configurations that are coming from specific geometry or limit positions. The complete dimensional synthesis is also presented

Diseño cinemático de un robot paralelo 2-PRR

Este artículo presenta una síntesis dimensional para un robot plano paralelo 2-PRR con estructura simétrica. Este robot posibilita la translación de la mesa móvil sin cambios en la orientación, lo cual resulta útil en aplicaciones que requieren posiciones controladas con alta rigidez. Debido a la alta sensibilidad que tienen estos robots con respecto a los cambios geométricos, muchas metodologías han sido desarrolladas para establecer la síntesis dimensional. En este trabajo se usó el Método de Normalización de Parámetros Finitos (PFNM) para establecer las dimensiones usando dos atlas de diseño, para el Índice Globales de Condición (GCI), y para el espacio de trabajo ( ). Se muestra que, para los dos atlas de diseño, GCI y , no es posible maximizar uno de los índices sin disminuir el otro, lo que representa un compromiso de diseño. Igualmente, se resaltan configuraciones singulares que vienen de geometrías especificas o de posiciones limite. La síntesis dimensional completa del 2-PRR es también presentada.In civil construction Abstract— This paper presents a dimensional synthesis for a 2-PRR planar parallel robot with a structural plane of symmetry. This robot can achieve the translation of the moving platform without changing the orientation, being useful for applications that require controlled positions with high rigidity. Because the performance of parallel robots is highly sensitive to their geometric parameters, many methodologies to state the dimensional synthesis has been developed. We used the method of Parameter - Finiteness Normalization Method (PFNM) to state the dimensional synthesis using Global Condition Index (GCI) and workspace ( ) design atlases. For the two, GCI and , designed atlases, it is not possible to maximize one of the indexes without diminishing the other one, which represents a design compromise. Also, we remark singular configurations that are coming from specific geometry or limit positions. The complete dimensional synthesis is also presented