807 research outputs found
Reliable microsatellite genotyping of the Eurasian badger (Meles meles) using faecal DNA
The potential link between badgers and bovine tuberculosis has made it vital to develop
accurate techniques to census badgers. Here we investigate the potential of using genetic
profiles obtained from faecal DNA as a basis for population size estimation. After trialling
several methods we obtained a high amplification success rate (89%) by storing faeces in
70% ethanol and using the guanidine thiocyanate/silica method for extraction. Using 70%
ethanol as a storage agent had the advantage of it being an antiseptic. In order to obtain reliable
genotypes with fewer amplification reactions than the standard multiple-tubes
approach, we devised a comparative approach in which genetic profiles were compared
and replication directed at similar, but not identical, genotypes. This modified method
achieved a reduction in polymerase chain reactions comparable with the maximumlikelihood
model when just using reliability criteria, and was slightly better when using
reliability criteria with the additional proviso that alleles must be observed twice to be considered
reliable. Our comparative approach would be best suited for studies that include
multiple faeces from each individual. We utilized our approach in a well-studied population
of badgers from which individuals had been sampled and reliable genotypes obtained.
In a study of 53 faeces sampled from three social groups over 10 days, we found that direct
enumeration could not be used to estimate population size, but that the application of
mark–recapture models has the potential to provide more accurate results
The effects of an experimental programme to support students’ autonomy on the overt behaviours of physical education teachers
Although the benefits of autonomy supportive behaviours are now well established in the literature, very few studies have attempted to train teachers to offer a greater autonomy support to their students. In fact, none of these studies has been carried out in physical education (PE). The purpose of this study is to test the effects of an autonomy-supportive training on overt behaviours of teaching among PE teachers. The experimental group included two PE teachers who were first educated on the benefits of an autonomy supportive style and then followed an individualised guidance programme during the 8 lessons of a teaching cycle. Their behaviours were observed and rated along 3 categories (i.e., autonomy supportive, neutral and controlling) and were subsequently compared to those of three teachers who formed the control condition. The results showed that teachers in the experimental group used more autonomy supportive and neutral behaviours than those in the control group, but no difference emerged in relation to controlling behaviours. We discuss the implications for schools of our findings
Recommended from our members
Enrichment of CpG island shore region hypermethylation in epigenetic breast field cancerization
While changes in DNA methylation are known to occur early in breast carcinogenesis and the landscape of breast tumour DNA methylation is profoundly altered compared with normal tissue, there have been limited efforts to identify DNA methylation field cancerization effects in histologically normal breast tissue adjacent to tumour. Matched tumour, histologically normal tissue of the ipsilateral breast (ipsilateral-normal), and histologically normal tissue of the contralateral breast (contralateral-normal) were obtained from nine women undergoing bilateral mastectomy. Laser capture microdissection was used to select epithelial cells from normal tissue, and neoplastic cells from tumour for genome-scale measures of DNA methylation with the Illumina HumanMethylationEPIC array. We identified substantially more CpG loci that were differentially methylated between contralateral-normal and tumour (63,271 CpG loci q \u3c 0.01), than between ipsilateral-normal and tumour (38,346 CpG loci q \u3c 0.01). We identified differential methylation in ipsilateral-normal relative to contralateral-normal tissue (9,562 CpG loci p \u3c 0.01). In this comparison, hypomethylated loci were significantly enriched for breast cancer-relevant transcription factor binding sites including those for ESR1, FoxA1, and GATA3 and hypermethylated loci were significantly enriched for CpG island shore regions. In addition, progression of shore hypermethylation was observed in tumours compared to matched ipsilateral normal tissue, and these alterations tracked to several well-established tumour suppressor genes. Our results indicate an epigenetic field effect in surrounding histologically normal tissue. This work offers an opportunity to focus investigations of early DNA methylation alterations in breast carcinogenesis and potentially develop epigenetic biomarkers of disease risk
Ethnic inequalities and pathways to care in psychosis in England: a systematic review and meta-analysis
© The Author(s). 2018Background: As part of a national programme to tackle ethnic inequalities, we conducted a systematic review and meta-analysis of research on ethnic inequalities in pathways to care for adults with psychosis living in England and/or Wales. Methods: Nine databases were searched from inception to 03.07.17 for previous systematic reviews, including forward and backward citation tracking and a PROSPERO search to identify ongoing reviews. We then carried forward relevant primary studies from included reviews (with the latest meta-analyses reporting on research up to 2012), supplemented by a search on 18.10.17 in MEDLINE, Embase, PsycINFO and CINAHL for primary studies between 2012 and 2017 that had not been covered by previous meta-analyses. Results: Forty studies, all conducted in England, were included for our updated meta-analyses on pathways to care. Relative to the White reference group, elevated rates of civil detentions were found for Black Caribbean (OR = 3.43, 95% CI = 2.68 to 4.40, n = 18), Black African (OR = 3.11, 95% CI = 2.40 to 4.02, n = 6), and South Asian patients (OR = 1.50, 95% CI 1.07 to 2.12, n = 10). Analyses of each Mental Health Act section revealed significantly higher rates for Black people under (civil) Section 2 (OR = 1.53, 95% CI = 1.11 to 2.11, n = 3). Rates in repeat admissions were significantly higher than in first admission for South Asian patients (between-group difference p < 0.01). Some ethnic groups had more police contact (Black African OR = 3.60, 95% CI = 2.15 to 6.05, n = 2; Black Caribbean OR = 2.64, 95% CI = 1.88 to 3.72, n = 8) and criminal justice system involvement (Black Caribbean OR = 2.76, 95% CI = 2.02 to 3.78, n = 5; Black African OR = 1.92, 95% CI = 1.32 to 2.78, n = 3). The White Other patients also showed greater police and criminal justice system involvement than White British patients (OR = 1.49, 95% CI = 1.03 to 2.15, n = 4). General practitioner involvement was less likely for Black than the White reference group. No significant variations over time were found across all the main outcomes. Conclusions: Our updated meta-analyses reveal persisting but not significantly worsening patterns of ethnic inequalities in pathways to psychiatric care, particularly affecting Black groups. This provides a comprehensive evidence base from which to inform policy and practice amidst a prospective Mental Health Act reform. Trial registration: CRD42017071663Peer reviewedFinal Published versio
Seasonal Distribution, Aggregation, and Habitat Selection of Common Carp in Clear Lake, Iowa
The common carp Cyprinus carpio is widely distributed and frequently considered a nuisance species outside its native range. Common carp are abundant in Clear Lake, Iowa, where their presence is both a symptom of degradation and an impediment to improving water quality and the sport fishery. We used radiotelemetry to quantify seasonal distribution, aggregation, and habitat selection of adult and subadult common carp in Clear Lake during 2005–2006 in an effort to guide future control strategies. Over a 22-month period, we recorded 1,951 locations of 54 adults and 60 subadults implanted with radio transmitters. Adults demonstrated a clear tendency to aggregate in an offshore area during the late fall and winter and in shallow, vegetated areas before and during spring spawning. Late-fall and winter aggregations were estimated to include a larger percentage of the tracked adults than spring aggregations. Subadults aggregated in shallow, vegetated areas during the spring and early summer. Our study, when considered in combination with previous research, suggests repeatable patterns of distribution, aggregation, and habitat selection that should facilitate common carp reduction programs in Clear Lake and similar systems
Search for the lepton-family-number nonconserving decay \mu -> e + \gamma
The MEGA experiment, which searched for the muon- and electron-number
violating decay \mu -> e + \gamma, is described. The spectrometer system, the
calibrations, the data taking procedures, the data analysis, and the
sensitivity of the experiment are discussed. The most stringent upper limit on
the branching ratio of \mu -> e + \gamma) < 1.2 x 10^{-11} was obtained
Recommended from our members
Solar neutrino with Borexino: results and perspectives
Borexino is a unique detector able to perform measurement of solar neutrinos
fluxes in the energy region around 1 MeV or below due to its low level of
radioactive background. It was constructed at the LNGS underground laboratory
with a goal of solar Be neutrino flux measurement with 5\% precision. The
goal has been successfully achieved marking the end of the first stage of the
experiment. A number of other important measurements of solar neutrino fluxes
have been performed during the first stage. Recently the collaboration
conducted successful liquid scintillator repurification campaign aiming to
reduce main contaminants in the sub-MeV energy range. With the new levels of
radiopurity Borexino can improve existing and challenge a number of new
measurements including: improvement of the results on the Solar and terrestrial
neutrino fluxes measurements; measurement of pp and CNO solar neutrino fluxes;
search for non-standard interactions of neutrino; study of the neutrino
oscillations on the short baseline with an artificial neutrino source (search
for sterile neutrino) in context of SOX project.Comment: 15 pages, 4 figure
Recent Borexino results and prospects for the near future
The Borexino experiment, located in the Gran Sasso National Laboratory, is an
organic liquid scintillator detector conceived for the real time spectroscopy
of low energy solar neutrinos. The data taking campaign phase I (2007 - 2010)
has allowed the first independent measurements of 7Be, 8B and pep fluxes as
well as the first measurement of anti-neutrinos from the earth. After a
purification of the scintillator, Borexino is now in phase II since 2011. We
review here the recent results achieved during 2013, concerning the seasonal
modulation in the 7Be signal, the study of cosmogenic backgrounds and the
updated measurement of geo-neutrinos. We also review the upcoming measurements
from phase II data (pp, pep, CNO) and the project SOX devoted to the study of
sterile neutrinos via the use of a 51Cr neutrino source and a 144Ce-144Pr
antineutrino source placed in close proximity of the active material.Comment: 8 pages, 11 figures. To be published as proceedings of Rencontres de
Moriond EW 201
Low-energy (anti)neutrino physics with Borexino: Neutrinos from the primary proton-proton fusion process in the Sun
The Sun is fueled by a series of nuclear reactions that produce the energy
that makes it shine. The primary reaction is the fusion of two protons into a
deuteron, a positron and a neutrino. These neutrinos constitute the vast
majority of neutrinos reaching Earth, providing us with key information about
what goes on at the core of our star. Several experiments have now confirmed
the observation of neutrino oscillations by detecting neutrinos from secondary
nuclear processes in the Sun; this is the first direct spectral measurement of
the neutrinos from the keystone proton-proton fusion. This observation is a
crucial step towards the completion of the spectroscopy of pp-chain neutrinos,
as well as further validation of the LMA-MSW model of neutrino oscillations.Comment: Proceedings from NOW (Neutrino Oscillation Workshop) 201
Final results of Borexino Phase-I on low energy solar neutrino spectroscopy
Borexino has been running since May 2007 at the LNGS with the primary goal of
detecting solar neutrinos. The detector, a large, unsegmented liquid
scintillator calorimeter characterized by unprecedented low levels of intrinsic
radioactivity, is optimized for the study of the lower energy part of the
spectrum. During the Phase-I (2007-2010) Borexino first detected and then
precisely measured the flux of the 7Be solar neutrinos, ruled out any
significant day-night asymmetry of their interaction rate, made the first
direct observation of the pep neutrinos, and set the tightest upper limit on
the flux of CNO neutrinos. In this paper we discuss the signal signature and
provide a comprehensive description of the backgrounds, quantify their event
rates, describe the methods for their identification, selection or subtraction,
and describe data analysis. Key features are an extensive in situ calibration
program using radioactive sources, the detailed modeling of the detector
response, the ability to define an innermost fiducial volume with extremely low
background via software cuts, and the excellent pulse-shape discrimination
capability of the scintillator that allows particle identification. We report a
measurement of the annual modulation of the 7 Be neutrino interaction rate. The
period, the amplitude, and the phase of the observed modulation are consistent
with the solar origin of these events, and the absence of their annual
modulation is rejected with higher than 99% C.L. The physics implications of
phase-I results in the context of the neutrino oscillation physics and solar
models are presented
- …