Blocking two-component signalling enhances Candida albicans virulence and reveals adaptive mechanisms that counteract sustained SAPK activation

This work was funded by the UK Biotechnology and Biological Research Council [www.bbsrc.ac.uk] JQ (BB/K016393/1); AJPB (BB/K017365/1). The work was also supported by the Wellcome Trust [www.wellcome.ac.uk], JQ (086048, 097377); AJPB (097377)); LPE (097377). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewedPublisher PD

Integrative Model of Oxidative Stress Adaptation in the Fungal Pathogen Candida albicans

Acknowledgments We are grateful to the Ian Fraser Cytometry Centre and our Mass Spetrometry and qPCR Facilities for help with the flow cytometry, glutathione and qRT-PCR assays, respectively. We also thank our many colleagues in the CRISP Consortium and in the medical mycology and systems biology communities for insightful discussions. Funding: This work was supported by the CRISP project (Combinatorial Responses In Stress Pathways), which was funded by the UK Biotechnology and Biological Research Council (www.bbsrc.ac.uk): AJPB, KH, CG, ADM, NARG, MT, MCR. (Research Grants; BB/F00513X/1, BB/F005210/1-2). AJPB and JQ received additional support from the BBSRC (Research Grants; BB/K016393/1; BB/K017365/1). NARG and AJPB were also supported by the Wellcome Trust (www.wellcome.ac.uk), (Grants: 080088; 097377). AJPB was also supported by the European Research Council (http://erc.europa.eu/), (STRIFE Advanced Grant; ERC-2009-AdG-249793). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewedPublisher PD

Pho4 mediates phosphate acquisition in Candida albicans and is vital for stress resistance and metal homeostasis

ACKNOWLEDGMENTS We thank Karl Kuchler for the C. albicans superoxide dismutase mutants used in this study. This work was funded by a Medical Research Council Doctoral Training Program studentship to M.A.C.I.; Wellcome Trust Grants 089930 to J.Q., 080088 to A.J.P.B., and 097377 to J.Q., A.J.P.B., and L.P.E.; Biotechnology and Biological Sciences Research Council Grants BB/K016393/1 to J.Q. and BB/F00513X/1 and BB/K017365/1 to A.J.P.B.; European Research Council STRIFE Advanced Grant ERC-2009-AdG-249793 to A.J.P.B.; and Wellcome Trust and Royal Society Sir Henry Dale Fellowship 098375/Z/12/Z to K.J.W. and E.T. The funders had no role in study design, data collection, or interpretation or the decision to submit the work for publication.Peer reviewedPublisher PD

Global overview of the management of acute cholecystitis during the COVID-19 pandemic (CHOLECOVID study)

Background: This study provides a global overview of the management of patients with acute cholecystitis during the initial phase of the COVID-19 pandemic. Methods: CHOLECOVID is an international, multicentre, observational comparative study of patients admitted to hospital with acute cholecystitis during the COVID-19 pandemic. Data on management were collected for a 2-month study interval coincident with the WHO declaration of the SARS-CoV-2 pandemic and compared with an equivalent pre-pandemic time interval. Mediation analysis examined the influence of SARS-COV-2 infection on 30-day mortality. Results: This study collected data on 9783 patients with acute cholecystitis admitted to 247 hospitals across the world. The pandemic was associated with reduced availability of surgical workforce and operating facilities globally, a significant shift to worse severity of disease, and increased use of conservative management. There was a reduction (both absolute and proportionate) in the number of patients undergoing cholecystectomy from 3095 patients (56.2 per cent) pre-pandemic to 1998 patients (46.2 per cent) during the pandemic but there was no difference in 30-day all-cause mortality after cholecystectomy comparing the pre-pandemic interval with the pandemic (13 patients (0.4 per cent) pre-pandemic to 13 patients (0.6 per cent) pandemic; P = 0.355). In mediation analysis, an admission with acute cholecystitis during the pandemic was associated with a non-significant increased risk of death (OR 1.29, 95 per cent c.i. 0.93 to 1.79, P = 0.121). Conclusion: CHOLECOVID provides a unique overview of the treatment of patients with cholecystitis across the globe during the first months of the SARS-CoV-2 pandemic. The study highlights the need for system resilience in retention of elective surgical activity. Cholecystectomy was associated with a low risk of mortality and deferral of treatment results in an increase in avoidable morbidity that represents the non-COVID cost of this pandemic

Melanoma pulmonar primario: a propósito de un caso.

We present the case of a primary lung melanoma in a 73-year-old man. The diagnosis was finally obtained after the surgical removal of the lung mass and after having reasonably ruled out the existence of malignant melanoma at any other level. It is a really rare entity, accounting for 0.01% of malignant lung tumors

Prevalencia de psicopatías en un centro de salud rural

Objetivo. Conocer la prevalencia de psicopatología en un centro de salud rural y comparar los resultados con los obtenidos en estudios de similar diseño. Diseño. Estudio de prevalencia, transversal, de doble fase: primera de detección y segunda de evaluación psicopatológica. Emplazamiento. Centro de Salud del Marquesado (Granada). Población rural dispersa con bajo nivel socioeconómico y alto porcentaje de ancianos. Participantes. Muestra aleatoria simple representativa de la población mayor de 18 años de la comarca. N = 245. Error a = 5%. Fuente de muestreo: censo electoral. Mediciones. Cuestionario GHQ-28 de Goldberg, datos sociodemográficos y test de CAGE. A los que superaron el punto de corte (GHQ-28 >= 5) se les administró el cuestionario CIS por psiquiatra, asignándoseles un diagnóstico CIE-10. Si CAGE >= 2 se estudió consumo perjudicial de alcohol. Si había diagnostico previo, se obvió la segunda fase. Se obtienen prevalencias con su intervalo de confianza exacto. Resultados. Prevalencia de psicopatología: 28,6% (34,5% en mujeres y 22,6% en varones). Un 30% de los casos obtuvo 2 diagnósticos. La patología más prevalente la constituyen los trastornos por ansiedad (8,97%), que representan el 23,9% de los diagnósticos agrupados. Los trastornos depresivos tienen una prevalencia del 8,16% y el consumo problemático de sustancias un 6,12%. El diagnóstico aislado más frecuente es el de distimia (9,78% de los casos). La morbilidad psiquiátrica oculta es del 40%. Conclusiones. La prevalencia de psicopatología es muy elevada. Los trastornos más frecuentes son los ansiosos, seguidos de los depresivos y el consumo perjudicial de alcohol

Anales de Edafología y Fisiología Vegetal Tomo 17 Número 1

Sobre la purificación de algunos reactivos y electrodos ·para su empleo en análisis espectroquímicos de suelos, por Fernando Burriel Martí y Carmen Alvarez Herrero.-- Estudio mineralógico comparativo de la fracción arcilla de· algunos sedimentos del litoral español, por Julia Mª González Peña.-- Apéndice: Mineralogía de la fracción arena de los sedimentos marinos que se estudian, por Joséfina Pérez Mateos.-- Los suelos de las Islas Baleares, por H. Klinge y A. Mella.-- Efecto del tratamiento térmico y del contenido en Al2 03 sobre la superficie específica de los geles de sílice-alúmina, por Juan de Dios López González y Andrés Mata Arjona.-- Hidrólisis de la rafinosa por la melibiasa de levadura y fermentación de dicho azúcar por acción complementaria de genes, por Manuel Losada.-- Bibliografía. Pontificiae Academiae Scientiarum: Semaine d'etude sur le probleme des oligoelements dans la vi e vegetale et animalePeer reviewe