Evaluación del área trabecular por digitalización de imágenes en el callo de fractura: estudio experimental

Se presenta un método de evaluación del área trabecular por medio de digitalización de imágenes de microscopía óptica. Se estudia la evolución de 20 callos de fractura en ratas de cepa Wistar, obteniendo un incremento progresivo del área trabecular desde los 14 a los 49 días, correlacionando ésta directamente con la actividad neoformativa. Se concluye proponiendo el uso de este método por su uniformidad, objetividad y reproductibilidad en todo el estudio, permitiendo la independencia del anatomopatológico.We present a method for assessment of the bone trabecular area by using optic microscope digitalized imaging. We studied 20 fracture callus performed at the tibia in Wistar rats. The bone trabecular area had a progressive increased from 14 to 49 days, post-fracture, been directly related with the neoformative activity. We propose this method because of this uniformity, objetivity and reliability. This method akows fracture repair analysis independently of the subjetivity of the pathologist

Técnica de Imhäuser en el pie zambo : resultados a medio y largo plazo

Se revisan un total de 60 pies con equinovaro congénito pertenecientes a 48 pacientes, todos con un seguimiento superior a 5 años. En todos los casos se realizó un tratamiento combinado: ortopédico (manipulaciones y enyesados desde el nacimiento) y quirúrgico (correcciones según el método de Imhäuser modificado). Se valoró morfológica, funcional y radiográficamente cada caso. En la valoración global sólo 1 caso fue considerado malo, 13 regulares (21,6%), 6 por pies planos valgos, 3 por pronato de antepié y 4 por adducto de antepié, y 12 buenos (20%), aunque presentaban alguna alteración morfológica o funcional mínima, y 34 excelentes (56,6%). Se concluye que: 1) la técnica de Imhäuser ofrece en este trabajo el 77% de excelentes y buenos resultados; 2) el 2.° y 3.cr se pueden resumir en uno solo, el hemitrasplante del tibial anterior, y 3) los resultados clínicos, morfológicos y funcionales son superiores a los radiográficos.—We cheked 42 patients with club-feet (60 feet), all of them with follow-up over 5 years. All the patients received a mixed treatment: orthopaedic (manipulation and plastering) and surgical (Imhäuser technique modified). We evaluated morphological, functional and radiographic parameters. In the overall evaluation only one of the cases was considered bad result; 13 (21.6%) fair results, 6 with valgus flat feet, 3 with pronatus forefoot and 4 with adductus forefoot; 12 (20%) good results, with a minimal morphologic or functional alterations; 34 (56.6%) were excellents results. Conclutions: 1) Imhäuser technic had 77% of good or excellent results in this work; 2) the second and third Imhäuser surgical times can be summarized in only one: the external hemitrasplant of the tibial anterior tendon, and 3) clinics, morphologics and functional results are better than radiographics results

The wideband backend at the MDSCC in Robledo. A new facility for radio astronomy at Q- and K- bands

The antennas of NASA's Madrid Deep Space Communications Complex (MDSCC) in Robledo de Chavela are available as single-dish radio astronomical facilities during a significant percentage of their operational time. Current instrumentation includes two antennas of 70 and 34 m in diameter, equipped with dual-polarization receivers in K (18 - 26 GHz) and Q (38 - 50 GHz) bands, respectively. We have developed and built a new wideband backend for the Robledo antennas, with the objectives (1) to optimize the available time and enhance the efficiency of radio astronomy in MDSCC; and (2) to tackle new scientific cases impossible to that were investigated with the old, narrow-band autocorrelator. The backend consists of an IF processor, a FFT spectrometer (FFTS), and the software that interfaces and manages the events among the observing program, antenna control, the IF processor, the FFTS operation, and data recording. The whole system was end-to-end assembled in August 2011, at the start of commissioning activities, and the results are reported in this paper. Frequency tunings and line intensities are stable over hours, even when using different synthesizers and IF channels; no aliasing effects have been measured, and the rejection of the image sideband was characterized. The first setup provides 1.5 GHz of instantaneous bandwidth in a single polarization, using 8192 channels and a frequency resolution of 212 kHz; upgrades under way include a second FFTS card, and two high-resolution cores providing 100 MHz and 500 MHz of bandwidth, and 16384 channels. These upgrades will permit simultaneous observations of the two polarizations with instantaneous bandwidths from 100 MHz to 3 GHz, and spectral resolutions from 7 to 212 kHz.Comment: 9 pages, 8 figures. Accepted to Astronomy and Astrophysic

Rv2577 of Mycobacterium tuberculosis is a virulence factor with dual phosphatase and phosphodiesterase functions

Tuberculosis, a lung disease caused by Mycobacterium tuberculosis (Mtb), is one of the ten leading causes of death worldwide affecting mainly developing countries. Mtb can persist and survive inside infected cells through modulation of host antibacterial attack, i.e., by avoiding the maturation of phagosome containing mycobacteria to more acidic endosomal compartment. In addition, bacterial phosphatases play a central role in the interplay between host cells and Mtb. In this study, we characterized the Rv2577 of Mtb as a potential alkaline phosphatase/phosphodiesterase enzyme. By an in vitro kinetic assay, we demonstrated that purified Rv2577 expressed in Mycobacterium smegmatis displays both enzyme activities, as evidenced by using the artificial substrates p-NPP and bis-(p-NPP). In addition, a three-dimensional model of Rv2577 allowed us to define the catalytic amino acid residues of the active site, which were confirmed by site-directed mutagenesis and enzyme activity analysis, being characteristic of a member of the metallophosphatase superfamily. Finally, a mutation introduced in Rv2577 reduced the replication of Mtb in mouse organs and impaired the arrest of phagosomes containing mycobacteria in early endosomes; which indicates Rv2577 plays a role in Mtb virulence.Instituto de BiotecnologíaFil: Forrellad, Marina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marrero Diaz de Villegas, Rubén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Yaneff, Agustín. Universidad de Buenos Aires. Instituto de Investigaciones Farmacológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gutierrez, Maximiliano Gabriel. The Francis Crick Institute, Host-Pathogen Interactions in Tuberculosis Laboratory; Reino UnidoFil: Durán, Rosario. Institut Pasteur de Montevideo; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; UruguayFil: Villarino, Andrea. Universidad de la República (UdelaR). Facultad de Ciencias. Sección Bioquímica; UruguayFil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de investigaciones Científicas y Tecnológicas; Argentin

Rv2577 of mycobacterium tuberculosis Is a virulence factor with dual phosphatase and phosphodiesterase functions

Tuberculosis, a lung disease caused by Mycobacterium tuberculosis (Mtb), is one of the ten leading causes of death worldwide affecting mainly developing countries. Mtb can persist and survive inside infected cells through modulation of host antibacterial attack, i.e., by avoiding the maturation of phagosome containing mycobacteria to more acidic endosomal compartment. In addition, bacterial phosphatases play a central role in the interplay between host cells and Mtb. In this study, we characterized the Rv2577 of Mtb as a potential alkaline phosphatase/phosphodiesterase enzyme. By an in vitro kinetic assay, we demonstrated that purified Rv2577 expressed in Mycobacterium smegmatis displays both enzyme activities, as evidenced by using the artificial substrates p-NPP and bis-(p-NPP). In addition, a three-dimensional model of Rv2577 allowed us to define the catalytic amino acid residues of the active site, which were confirmed by site-directed mutagenesis and enzyme activity analysis, being characteristic of a member of the metallophosphatase superfamily. Finally, a mutation introduced in Rv2577 reduced the replication of Mtb in mouse organs and impaired the arrest of phagosomes containing mycobacteria in early endosomes; which indicates Rv2577 plays a role in Mtb virulence

Susceptibility of biological stages of the horn fly, Haematobia irritans, to entomopathogenic fungi (Hyphomycetes)

The susceptibility of the egg, pupa, and adult of Haematobia irritans (L.) (Diptera: Muscidae) to isolates of the fungi Metarhizium anisopliae (Metsch.) Sor., Beauveria bassiana (Bals.) Vuill., and Paecilomyces fumosoroseus (Wize) Brown and Smith, was evaluated under laboratory conditions. Groups of 20 eggs than 4 h old, pupae less than 48h old and adults were sprayed with a conidial suspension of each isolate. Eggs, pupae and adults of horn fly were susceptible to these entomopathogenic fungi. For treated eggs, the isolates Ma3, Ma 15, Ma25, Pfr1, and Pfr8 reduced adult emergence to 3.8% to 6.3% in comparison with the control (72%). The mortality of pupae infected by the isolates Ma2, Ma25, and Pfr10 ranged between 50% and 71.3%. Mortality of adults after treatment with the isolates Ma6, Ma 10, Ma 14, Ma 15, Pfr 1, Pfr 9, Pfr 10, Pfr 11, and Pfr12 were higher than 90%. The isolate Ma6 produced the lowest LC(50) against adult horn flies (8.08 &times 10(2)conidia/ml). These findings supported the hypotheses that isolates of M. anisopliae, and P. fumosoroseus are pathogenic against the different biological stages of horn flies by reducing adult emergence when applied on groups of eggs and pupae, and producing mortality when applied to adults

Proyecto de emprendimiento para la venta de artesanía textil mexicana

En este trabajo se presenta un modelo de negocios para comercializar textiles artesanales mexicanos en el mercado europeo a través de una plataforma web de comercio electrónico. Para la creación de este proyecto, se realizó un análisis de la industria textil y de la comercialización de artesanías, un estudio de mercado, del mercado meta y de las tendencias. De igual forma, se muestra el plan estratégico de acción mediante el cual se pretende implementar y darle seguimiento al negocio

The OCCASO survey: presentation and radial velocities of 12 Milky Way open clusters

Open clusters (OCs) are crucial for studying the formation and evolution of the Galactic disc. However, the lack of a large number of OCs analysed homogeneously hampers the investigations about chemical patterns and the existence of Galactocentric radial and vertical gradients, or an age-metallicity relation. To overcome this, we have designed the Open Cluster Chemical Abundances from Spanish Observatories (OCCASO) survey. We aim to provide homogeneous radial velocities, physical parameters and individual chemical abundances of six or more red clump stars for a sample of 25 old and intermediate-age OCs visible from the Northern hemisphere. To do so, we use high-resolution spectroscopic facilities (R >= 62 000) available at Spanish observatories. We present the motivation, design and current status of the survey, together with the first data release of radial velocities for 77 stars in 12 OCs, which represents about 50 per cent of the survey. We include clusters never studied with high-resolution spectroscopy before (NGC 1907, NGC 6991, NGC 7762), and clusters in common with other large spectroscopic surveys like the Gaia-ESO Survey (NGC 6705) and Apache Point Observatory Galactic Evolution Experiment (NGC 2682 and NGC 6819). We perform internal comparisons between instruments to evaluate and correct internal systematics of the results, and compare our radial velocities with previous determinations in the literature, when available. Finally, radial velocities for each cluster are used to perform a preliminary kinematic study in relation with the Galactic disc