Pharmacognostical and phytochemical standardization of Baladi churna- A polyherbal formulation

Baladi Churna described by Acharya Bhavprkash in Vandhyatva (infertility). Endometrium dysfunction is the prime cause of Infertility among the world Baladi Churna is useful in patients especially having implantation failure due to defective endometrium, which is known as Kshetra deformity in Ayurveda. So a new pharmaceutical preparation Baladi churna was tried to standardize which is economical in terms of time and machinery usage. The present work was carried out to standardize the finished product of to confirm Baladi Churna entity, quality and purity. The presence of trichomes, spiral vessels, pollen grains were the characteristic features observed in the microscopy of the prepared drug. Phyto-chemical analysis showed Loss on drying 8.24% w/w, ash value 9 %w/w, water soluble extract 46.3 % w/w and pH 6.5. HPTLC of Baladi Churna is the preliminary quantitative analysis which shows 11 prominent spots in UV 254 nm and 10 prominent spots 366 nm. Baladi Churna, a polyherbal formulation of 6 ingredients was prepared and HPTLC finger print profile was developed and it can be considered pharmacopial standard of Baladi Churna

Agroecological management of fall armyworm using soil and botanical treatments reduces crop damage and increases maize yield

IntroductionFall armyworm continues to disrupt smallholder farming systems across sub-Saharan Africa, with sporadic outbreaks and chronic cereal crop losses. Smallholders have been adapting to the pest by increasing crop surveillance for targeted control measures and developing low-cost solutions. For example, some report placing soil or ash in maize whorls where the mechanism of pest control may be suffocation, abrasion leading to desiccation, or through the introduction of soil-borne entomopathogens.MethodsTo verify the efficacy of this approach we evaluated different soil types on maize infested with fall armyworm to assess their efficacy. We also evaluated the efficacy of pesticidal plant species, powdered and placed in leaf whorls to control fall armyworm. Results and discussionDifferent United States Department of Agriculture-characterised soil types (sand, loam, clay) and wood ash were effective in reducing the number of larvae and maize leaf damage by approximately 50%. Maize yield with the synthetic control (chlorpyriphos) was 13,700 kg/ha, which was 42% higher than the untreated control (7,900 kg/ha). Soil and ash treatments yields between 10,400 to 12,400 kg/ha were 24-36% higher than the untreated control. Dry soil applied after watering was most effective regardless of soil type. However, wet soil treatments applied before watering were also highly effective in reducing the number of fall armyworm larvae and reducing insect damage to maize leaves. Botanical powders from Azadirachta indica, Nicotiana tabacum, Cymbopogon citratus and Lippia javanica were also effective when applied to maize leaf whorls. Plant powder treatments and water extracts were significantly effective in reducing the number of larvae and leaf damage. The highest yield obtained with botanicals was observed with A. indica powder (5,600 kg/ha), C. citratus extract (5,800 kg/ha) and N. tabacum extract (5,800 kg/ha), where the synthetic treatment yield was 6,900 kg/ha and the untreated yield was 1,700 kg/ha. We conclude that smallholder farmer innovations in managing fall armyworm are effective low-cost options. Scientific validation of soil treatments and botanicals should help increase the confidence of policy makers and allow knowledge extension services to recommend their use to smallholder farmers, which in turn may reduce reliance on imported synthetic pesticides and improve farmer resilience, circular economies and human and environmental health

The Role of Drought Stress on Aflatoxin Contamination in Groundnuts (Arachis hypogea L.) and Aspergillus flavus Population in the Soil

Aflatoxins are naturally occurring toxic chemical substances that are produced by fungal species called Aspergillus flavus. The toxic substances are secondary metabolites, which contaminate groundnut while growing in the field and also post-harvest. Drought stress is one of the factors that contribute to increased aflatoxin levels in groundnut during field production. This study was conducted in a screen house at ICRISAT-Chitedze Agricultural Research Station, Malawi to investigate the effects of drought on aflatoxin contamination and A.flavus population in the soil. Four drought stress levels; prolonged (4 weeks), min (3 weeks), mild (2 weeks) and no drought were imposed on five groundnut varieties at pod filling stage. Soil samples were collected from each plot four times; at planting, beginning of drought, end of drought and at harvest. Aflatoxin levels in groundnut grain samples were estimated by use of neogenstrips read with mobile assay tablet reader. Population densities of A.flavus in soil samples collected from the plots were estimated using serial dilutions plated on the selective media, modified dichloran Rose Bengal (MDRB) and quantify A.flavus within 3 days after incubation at 37°C.The results showed that there were significant differences in aflatoxin contamination between drought stress levels (p = 0.011). High aflatoxin contamination was observed under prolonged drought (22.0 ppb) compared to and no drought treatment (1.5 ppb). None of the varieties used in the study showed either resistance or susceptibility to aflatoxin contamination under drought or adequate soil moisture. The results also showed that there were significant differences in A.flavus population at drought period and harvesting time and the mean population of A.flavus in prolonged drought at end of stress and harvesting were 8511 and 6044 cfu/g of soil respectively. It was concluded that drought contribute to aflatoxin contamination in groundnut, and also increased the A.flavus population in soil and also at harvesting

Predictive factors for sustained pain after (sub)acute osteoporotic vertebral fractures:Combined results from the VERTOS II and VERTOS IV trial

PURPOSE: Osteoporotic vertebral compression fractures are treated conservatively or in selected cases with percutaneous vertebroplasty (PV). The purpose of this retrospective analysis is to determine predictive factors for a high visual analogue scale (VAS) pain score after conservative, sham or PV and is based on previously published randomized trials. METHODS: The VERTOS II compared conservative versus PV, and VERTOS IV compared sham versus PV treatment. The conservative group received pain medication. The sham and PV group received subcutaneous lidocaine/bupivacaine. In addition, the PV group received cementation, which was simulated in the sham group. Nineteen different predictors of high (≥ 5) versus low ( 8, long-term baseline pain, mild/severe Genant and new fractures. CONCLUSIONS: Statistically significant more patients had a high pain score at 12 months in the sham and conservative group when compared with the PV group. Five predictors were identified for sustained high local back pain, regardless of the received treatment. Patients with moderate fracture deformity were less likely to have high pain scores at 12 months if they received PV than if they had sham or conservative therapy. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00270-022-03170-7

Sleep and energy intake in early childhood

Background And Objectives: Shorter sleep is associated with higher weight in children, but little is known about the mechanisms. The aim of this study was to test the hypothesis that shorter sleep was associated with higher energy intake in early childhood. Methods: Participants were 1303 families from the Gemini twin birth cohort. Sleep duration was measured using the Brief Infant Sleep Questionnaire when the children were 16 months old. Total energy intake (kcal per day) and grams per day of fat, carbohydrate and protein were derived from 3-day diet diaries completed by parents when children were 21 months old. Results: Shorter nighttime sleep was associated with higher total energy intake (P for linear trend=0.005). Children sleeping <10 h consumed around 50 kcal per day more than those sleeping 11–<12 h a night (the optimal sleep duration for children of this age). Differences in energy intake were maintained after adjustment for confounders. As a percentage of total energy intake, there were no significant differences in macronutrient intake by sleep duration. The association between sleep and weight was not significant at this age (P=0.13). Conclusions: This study provides the first evidence that shorter nighttime sleep duration has a linear association with higher energy intake early in life. That the effect is observed before emergence of associations between sleep and weight indicates that differences in energy intake may be a mechanism through which sleep influences weight gain

Excellent response to pasireotide therapy in an aggressive and dopamine-resistant prolactinoma.

peer reviewedProlactinomas are the most commonly encountered pituitary adenomas in the clinical setting. While most can be controlled by dopamine agonists, a subset of prolactinomas are dopamine-resistant and very aggressive. In such tumors, the treatment of choice is neurosurgery and radiotherapy, with or without temozolomide. Here, we report a patient with an highly aggressive, dopamine-resistant prolactinoma, who only achieved biochemical and tumor control during pasireotide long-acting release (PAS-LAR) therapy , a second-generation somatostatin receptor ligand (SRL). Interestingly, cystic degeneration, tumor cell necrosis, or both was observed after PAS-LAR administration suggesting an antitumor effect. This case shows that PAS-LAR therapy holds clinical potential in selective aggressive, dopamine-resistant prolactinomas that express somatostatin (SST) receptor subtype 5 and appears to be a potential new treatment option before starting temozolomide. In addition, PAS-LAR therapy may induce cystic degeneration, tumor cell necrosis, or both in prolactinomas

Synthesis and characterization of ZnO nanostructures using palm olein as biotemplate

Background: A green approach to synthesize nanomaterials using biotemplates has been subjected to intense research due to several advantages. Palm olein as a biotemplate offers the benefits of eco-friendliness, low-cost and scale-up for large scale production. Therefore, the effect of palm olein on morphology and surface properties of ZnO nanostructures were investigated. Results: The results indicate that palm olein as a biotemplate can be used to modify the shape and size of ZnO particles synthesized by hydrothermal method. Different morphology including flake-, flower- and three dimensional star-like structures were obtained. FTIR study indicated the reaction between carboxyl group of palm olein and zinc species had taken place. Specific surface area enhanced while no considerable change were observed in optical properties. Conclusion: Phase-pure ZnO particles were successfully synthesized using palm olein as soft biotemplating agent by hydrothermal method. The physico-chemical properties of the resulting ZnO particles can be tuned using the ratio of palm olein to Zn cation

Study of e+e−→ppˉe^+e^- \rightarrow p\bar{p} in the vicinity of ψ(3770)\psi(3770)

Using 2917 $\rm{pb}^{-1}$ of data accumulated at 3.773~$\rm{GeV}$, 44.5~$\rm{pb}^{-1}$ of data accumulated at 3.65~$\rm{GeV}$ and data accumulated during a $\psi(3770)$ line-shape scan with the BESIII detector, the reaction $e^+e^-\rightarrow p\bar{p}$ is studied considering a possible interference between resonant and continuum amplitudes. The cross section of $e^+e^-\rightarrow\psi(3770)\rightarrow p\bar{p}$, $\sigma(e^+e^-\rightarrow\psi(3770)\rightarrow p\bar{p})$, is found to have two solutions, determined to be ($0.059\pm0.032\pm0.012$) pb with the phase angle $\phi = (255.8\pm37.9\pm4.8)^\circ$ ($<$0.11 pb at the 90% confidence level), or $\sigma(e^+e^-\rightarrow\psi(3770)\rightarrow p\bar{p}) = (2.57\pm0.12\pm0.12$) pb with $\phi = (266.9\pm6.1\pm0.9)^\circ$ both of which agree with a destructive interference. Using the obtained cross section of $\psi(3770)\rightarrow p\bar{p}$, the cross section of $p\bar{p}\rightarrow \psi(3770)$, which is useful information for the future PANDA experiment, is estimated to be either ($9.8\pm5.7$) nb ($<17.2$ nb at 90% C.L.) or $(425.6\pm42.9)$ nb