DSS-13 26-meter antenna upgraded radiometer system

The Deep Space Station (DSS)-13 26-m antenna radiometer system was upgraded with an IBM-compatible computer-controlled configuration with improved supporting hardware and software. Software was generated to analyze results and correct for antenna mispointing, tropospheric loss, and other observing errors. This total power radiometer configuration provides a prototype for the new DSS-13 34-m antenna. The radiometer system is described in terms of the theory, instrumentation hardware, computer configuration, and operational features and performance. The system is used to obtain antenna efficiency and pointing model data and is useful for radio source calibrations required for radio astronomy. Some recent results are given

The Politics of Proselytizing: Europe After 1848 and the Development of Mormon Pre-Millennialism

In the second year of his reign as King of the Neo-Babylonian Empire, Nebuchadnezzar II dreamt of a large stone that rolled down the side of a mountain, shattered a great metallic statue, and grew into a mountain range large enough to cover the earth. The accomplished ruler, who went on to oversee the construction of the Hanging Gardens of Babylon, as well as the destruction of Jerusalem’s Jewish temple, was no revelator, and so enlisted Daniel, an exiled Jew, to interpret his dream. According to Daniel, the statue, divided into five parts from head to toe, portrayed mankind’s present and future kingdoms. The last, represented by the statue’s iron and clay feet, would be “a divided kingdom.” Unable to unify, “the kingdom will be partly strong and partly brittle. . . . [T]he people will be a mixture and will not remain united, any more than iron mixes with clay.”1 Once all five kingdoms had matured and fallen, God’s kingdom, the large, rolling stone, would destroy all vestiges of temporal rule and establish governance over the earth until the end of time. To the Old Testament king, Daniel’s interpretation meant little; to future religious groups, and especially for members of the early Church of Jesus Christ of Latter-day Saints (Mormons), Nebuchadnezzar’s dream served as a clear roadmap to the millennium

Geronimo Creek Outfall Replacement, The City of Seguin, Guadalupe County, Texas: An Intensive Cultural Resource Survey

The City of Seguin (City) is proposing to construct a new, replacement effluent outfall for the Geronimo Creek Wastewater Treatment Plant. The City also proposes to abandon the existing outfall structure and existing pipeline in place. The proposed project will involve the extension of an existing 24 inch (in) (61 centimeters [cm]) wastewater treatment plant effluent line for a length of 535 feet (ft.) (163 meters [m]). The line will terminate at a new outfall structure located on the east bank of Geronimo Creek, approximately 260 ft. (79 m) from its confluence with the Guadalupe River. Two new manholes will be constructed along the line at major bends in the line. Line depths will range from 9 to 28 ft. (2.7 to 8.5 m). A 40 ft. (12.2 m) wide permanent easement is proposed for the line, along with a 30 ft. (9.1 m) wide temporary easementfor construction purposes. The extent of the outfallstructure should be fully within the 40 ft. (12.2 m) wide easement. The area of potential effect (APE) is defined as an area measuring 535 ft. (163 m) in length, and 70 ft. (21.3 m) in width for a total of 0.85 acres, and generally follows the eastern bank of Geronimo Creek. The Texas Historical Commission (THC) reviewed the proposed development plans and determined that a cultural resource survey was necessary for this City proposed project as the area has potential for cultural resources. The City contracted with TRC Environmental Corporation (TRC) of Austin to conduct the intensive cultural resource survey of their proposed undertaking/APE. TRC archeologists consulted the THC Archeological Sites Atlas (THC Atlas), a database that contains previously documented cultural resource sites and locations of previously conducted archeological surveys, prior to the field investigations. A 1 mi. (1.6 km) radius search around the APE revealed one previously recorded cultural resource site, 41GU21. This prehistoric site is on a high terrace just west of Geronimo Creek and has been extensively disturbed during modern housing construction and is considered ineligible for inclusion in the National Register of Historic Places (NRHP). No previous cultural resources surveys have been conducted in the vicinity of the APE. Southwest of the APE and beyond the 1 mi. (1.6 km) radius was a Texas Department of Transportation survey conducted in 2007 by Moore Consulting, Inc. along Farm-to-Market road 477. No cultural resources were recorded in that linear investigation. On October 14 and 15, 2014, Mike Quigg, a TRC archeologists of the Planning, Permitting, and Licensing Practice of TRC’s office in Austin, conducted an intensive cultural resource survey along the 535 ft. (163 m) length of proposed APE. This included a 100 percent pedestrian survey, plus the mechanical excavation of four backhoe trenches (BT) within the APE to determine the presence/absence of any potential cultural resources within the APE. One deeply buried prehistoric site of potential significance was identified in two trenches (BTs 1 and 2) on the upper terrace along the APE. No cultural materials were recovered from two trenches (BTs 3 and 4) dug into the APE in the lower terrace. No historic structures were in the proposed APE. TRC recommends avoidance of the potentially significant, deep stratified prehistoric site. If the site cannot be avoided then TRC recommends further testing to assess the sites significance, and the potential to yield significant information important in prehistory, either locally, regionally or nationally as per its legal obligations under existing state guidelines. The City of Seguin reviewed the draft report, the archeological findings, and recommendations by TRC archeologists, and sought an alternate route for the proposed sewer line to avoid impact to cultural resource site 41GU168. The City and its engineers agreed to an alternate reroute satisfactory with the landowner that avoids the upper terrace The City of Seguin reviewed the draft report, the archeological findings, and recommendations by TRC archeologists, and sought an alternate route for the proposed sewer line to avoid impact to cultural resource site 41GU168. The City and its engineers agreed to an alternate reroute satisfactory with the landowner that avoids the upper terrace and the archeological site altogether. This proposed reroute successfully avoids and preserves deeply buried prehistoric site 41GU168 in place. TRC now recommends no further cultural resource investigation following the new proposed sewer line location

Radiation damage to nucleoprotein complexes in macromolecular crystallography

Significant progress has been made in macromolecular crystallography over recent years in both the understanding and mitigation of X-ray induced radiation damage when collecting diffraction data from crystalline proteins. In contrast, despite the large field that is productively engaged in the study of radiation chemistry of nucleic acids, particularly of DNA, there are currently very few X-ray crystallographic studies on radiation damage mechanisms in nucleic acids. Quantitative comparison of damage to protein and DNA crystals separately is challenging, but many of the issues are circumvented by studying pre-formed biological nucleoprotein complexes where direct comparison of each component can be made under the same controlled conditions. Here a model protein-DNA complex C.Esp1396I is employed to investigate specific damage mechanisms for protein and DNA in a biologically relevant complex over a large dose range (2.07-44.63 MGy). In order to allow a quantitative analysis of radiation damage sites from a complex series of macromolecular diffraction data, a computational method has been developed that is generally applicable to the field. Typical specific damage was observed for both the protein on particular amino acids and for the DNA on, for example, the cleavage of base-sugar N1-C and sugar-phosphate C-O bonds. Strikingly the DNA component was determined to be far more resistant to specific damage than the protein for the investigated dose range. At low doses the protein was observed to be susceptible to radiation damage while the DNA was far more resistant, damage only being observed at significantly higher doses

Fatty acids from the cyanobacterium Microcystis aeruginosa with potent inhibitory effects on fish gill Na+/K+-ATPase activity

Contains fulltext : 14258.pdf (publisher's version ) (Open Access

Big Hole (41TV2161): Two Stratigraphically Isolated Middle Holocene Components in Travis County, Texas Volume I

During April and May 2006, an archeological team from the Cultural Resources Section of the Planning, Permitting and Licensing Practice of TRC Environmental Corporation’s (TRC) Austin office conducted geoarcheological documentation and data recovery excavations at prehistoric site 41TV2161 (CSJ: 0440-06-006). Investigations were restricted to a 70 centimeter (cm) thick target zone between ca. 220 and 290 cm below surface (bs) on the western side of site 41TV2161 – the Big Hole site in eastern Travis County, Texas. This cultural investigation was necessary under the requirements of Section 106 of the National Historic Preservation Act (NHPA), the implementing regulations of 36CRF Part 800 and the Antiquities Code of Texas (Texas Natural Resource Code, Title 9, Chapter 191 as amended) to recover a sample of the significant cultural materials prior to destruction by planned construction of State Highway 130 (SH 130). The latter by a private construction firm – Lone Star Infrastructure. This necessary data recovery was for Texas Department of Transportation (TxDOT), Environmental (ENV) Affairs Division under a Scientific Services Contract No. 577XXSA003 (Work Authorization No. 57701SA003). Over the years since the original award, multiple work authorizations between TxDOT and TRC were implemented and completed towards specific aspects of the analyses and reporting. The final analyses and report were conducted under contract 57-3XXSA004 (Work Authorization 57-311SA004). All work was under Texas Antiquities Committee Permit No. 4064 issued by the Texas Historical Commission (THC) to J. Michael Quigg. Initially, an archeological crew from Hicks & Company encountered site 41TV2161 during an intensive cultural resource inventory conducted south of Pearce Lane along the planned construction zone of SH 130 in the fall of 2005. Following the initial site discovery, archeologists expanded their investigations to the west across the SH 130 right-of-way, and completed excavation of 10 backhoe trenches, 13 shovel tests, and 11 test units at site 41TV2161. The investigations encountered at least seven buried cultural features and 1,034 artifacts, some in relatively good context. The survey and testing report to TxDOT presented their findings and recommendations (Campbell et al. 2006). The ENV Affairs Division of TxDOT and the THC reviewed the initial findings and recommendations, and determined site 41TV2161 was eligible for listing on the National Register of Historic Places and as State Antiquities Landmark as the proposed roadway development was to directly impact this important site and further excavations were required. Subsequently, TRC archeologists led by Paul Matchen (Project Archeologist) and J. Michael Quigg (Principal Investigator) initiated data recovery excavations through the mechanical-removal of between 220 and 250 cm of sediment from a 30-by-40 meter (m) block area (roughly 3,000 m3). This was conducted to allow hand-excavations to start just above the deeply buried, roughly 70 cm thick targeted zone of cultural material. Mechanical stripping by Lone Star Infrastructure staff created a large hole with an irregular bottom that varied between 220 and 260 cmbs. To locate specific areas to initiate hand-excavations within the mechanically stripped area, a geophysical survey that employed ground penetrating radar (GPR) was conducted by Tiffany Osburn then with Geo-Marine in Plano, Texas. Over a dozen electronic anomalies were detected through the GPR investigation. Following processing, data filtering, and assessment, Osburn identified and ranked the anomalies for investigation. The highest ranked anomalies (1 through 8) were thought to have the greatest potential to represent cultural features. Anomalies 1 through 6 were selected and targeted through hand-excavations of 1-by-1 m units that formed continuous excavation blocks of various sizes. Blocks were designated A, B, C, D, E, and F. The type, nature, quantity, and context of encountered cultural materials in each block led the direction and expansion of each excavation block as needed. In total, TRC archeologists hand-excavated 38.5 m3 (150 m2) from a vertically narrow target zone within this deep, multicomponent and stratified prehistoric site. Hand-excavation in the two largest Blocks, B and D (51 m2 and 62 m2 respectively), revealed two vertically separate cultural components between roughly 220 and 290 cmbs. The younger component was restricted to Block B and yielded a Bell/Andice point and point base, plus a complete Big Sandy point. These points were associated with at least eight small burned rock features, one cluster of ground stone tools, limited quantities of lithic debitage, few formal chipped and ground stone tools, and a rare vertebrate faunal assemblage. Roughly 20 to 25 cm below the Bell/Andice component in Block B and across Block D was a component identified by a single corner-notched Martindale dart point. This point was associated with a scattered burned rocks, three charcoal stained hearth features, scattered animal, bird, and fish bones, mussel shells, and less than a dozen formal chipped and ground stone tools. Both identified components contained cultural materials in good stratigraphic context with high spatial integrity. Significant, both were radiocarbon dated by multiple charcoal samples to a narrow 200-year period between 5250 and 5450 B.P. during the middle Holocene. With exception of the well-preserved faunal assemblages, perishable materials were poorly preserved in the moist silty clay loam. Charcoal lacked structure and was reduced to dark stains. Microfossils (e.g., phytoliths and starch gains) were present, although in very limited numbers and deteriorated conditions. The four much smaller Blocks (A, C, E, and F) yielded various quantities of cultural material and features, but these blocks also lacked sufficient charcoal dates and diagnostic artifacts Those artifacts and samples were left unassigned and analyzed separately from the Bell/Andice and Martindale components. The two well-defined components in Blocks B and D are the focus of this technical report. The components provide very significant data towards understanding rare and poorly understood hunter-gatherer populations during late stages of the Altithermal climate period. This final report builds upon the interim report submitted to TxDOT (Quigg et al. 2007) that briefly described the methods, excavations, preliminary findings, initial results from six feasibility studies, and proposed an initial research design for data analyses. Context and integrity of the cultural materials in the two identified components was excellent. This rare circumstance combined with detailed artifact analyses, solid documentation of their ages through multiple radiocarbon dates, and multidisciplinary approach to analyses, allowed significant insights and contributions concerning the two populations involved. Results provide a greater understanding of human behaviors during a rarely identified time in Texas Prehistory. The cultural materials and various collected samples were temporarily curated at TRC’s Austin laboratory. Following completion of analyses and acceptance of this final report, the artifacts, paper records, photographs, and electronic database were permanently curated at the Center for Archaeological Studies (CAS) at Texas State University in San Marcos

Considering aspects of the 3Rs principles within experimental animal biology

The 3Rs – Replacement, Reduction and Refinement – are embedded into the legislation and guidelines governing the ethics of animal use in experiments. Here, we consider the advantages of adopting key aspects of the 3Rs into experimental biology, represented mainly by the fields of animal behaviour, neurobiology, physiology, toxicology and biomechanics. Replacing protected animals with less sentient forms or species, cells, tissues or computer modelling approaches has been broadly successful. However, many studies investigate specific models that exhibit a particular adaptation, or a species that is a target for conservation, such that their replacement is inappropriate. Regardless of the species used, refining procedures to ensure the health and well-being of animals prior to and during experiments is crucial for the integrity of the results and legitimacy of the science. Although the concepts of health and welfare are developed for model organisms, relatively little is known regarding non-traditional species that may be more ecologically relevant. Studies should reduce the number of experimental animals by employing the minimum suitable sample size. This is often calculated using power analyses, which is associated with making statistical inferences based on the P-value, yet P-values often leave scientists on shaky ground. We endorse focusing on effect sizes accompanied by confidence intervals as a more appropriate means of interpreting data; in turn, sample size could be calculated based on effect size precision. Ultimately, the appropriate employment of the 3Rs principles in experimental biology empowers scientists in justifying their research, and results in highe-rquality science

Sustained pupil responses are modulated by predictability of auditory sequences

The brain is highly sensitive to auditory regularities and exploits the predictable order of sounds in many situations, from parsing complex auditory scenes, to the acquisition of language. To understand the impact of stimulus predictability on perception, it is important to determine how the detection of predictable structure influences processing and attention. Here we use pupillometry to gain insight into the effect of sensory regularity on arousal. Pupillometry is a commonly used measure of salience and processing effort, with more perceptually salient or perceptually demanding stimuli consistently associated with larger pupil diameters.In two experiments we tracked human listeners' pupil dynamics while they listened to sequences of 50ms tone pips of different frequencies. The order of the tone pips was either random, contained deterministic (fully predictable) regularities (experiment 1, n = 18, 11 female) or had a probabilistic regularity structure (experiment 2, n = 20, 17 female). The sequences were rapid, preventing conscious tracking of sequence structure thus allowing us to focus on the automatic extraction of different types of regularities. We hypothesized that if regularity facilitates processing by reducing processing demands, a smaller pupil diameter would be seen in response to regular relative to random patterns. Conversely, if regularity is associated with heightened arousal and attention (i.e. engages processing resources) the opposite pattern would be expected. In both experiments we observed a smaller sustained (tonic) pupil diameter for regular compared with random sequences, consistent with the former hypothesis and confirming that predictability facilitates sequence processing.SIGNIFICANCE STATEMENTThe brain is highly sensitive to auditory regularities. To appreciate the impact that the presence of predictability has on perception, we need to better understand how a predictable structure influences processing and attention. We recorded listeners' pupil responses to sequences of tones that followed either a predictable or unpredictable pattern, as the pupil can be used to implicitly tap into these different cognitive processes. We found that the pupil showed a smaller sustained diameter to predictable sequences, indicating that predictability eased processing rather than boosted attention. The findings suggest that the pupil response can be used to study the automatic extraction of regularities, and that the effects are most consistent with predictability helping the listener to efficiently process upcoming sounds

Soluble and cell-associated transferrin receptor in lung cancer.

The expression of transferrin receptor (TfR) has been identified in many malignant tumours. In lung cancer, lymphoma and breast cancer, it has been shown that the expression of TfR correlates with tumour differentiation, probably implying some prognostic value. A soluble form of TfR (sTfR) in human serum has been shown to be proportional to the number of cellular TfRs. Based on these data we examined the utility of measuring sTfR in the serum and bronchoalveolar lavage (BAL) fluid of patients with lung cancer (n = 32) and patients with chronic obstructive pulmonary disease (n = 22). BAL fluid was centrifuged to separate the supernatant from the cellular component. Cells were lysed in a detergent and cell-associated TfR was measured by enzyme-linked immunosorbent assay (ELISA) and expressed as ng 10(-6) cells in this cellular component. There was no difference in serum sTfR between the cancer and chronic obstructive pulmonary disease (COPD) groups. A higher level of cell-associated TfR was found in BAL of non-small-cell lung cancer patients than in COPD patients (P = 0.01). The calculated number of TfR molecules per cell in BAL correlated positively with the percentage of macrophages in BAL (P < 0.0001), suggesting that cell-associated TfR in BAL originates primarily from macrophages in this fluid. No correlation existed between BAL cell-associated TfR and tumour size, nodal status, the presence of metastases and serum sTfR. BAL cell-associated TfR was negatively correlated with BAL supernatant neuron-specific enolase (NSE) (P = 0.01). A combination of BAL supernatant NSE and cell-associated TfR detected lung cancer with a sensitivity of 91%, a specificity of 59% and positive and negative predictive values of 81% and 71% respectively. In conclusion, BAL cell-associated TfR may help in the differential diagnosis of lung cancer vs pneumonia