Logarithmic diffusion and porous media equations: a unified description

In this work we present the logarithmic diffusion equation as a limit case when the index that characterizes a nonlinear Fokker-Planck equation, in its diffusive term, goes to zero. A linear drift and a source term are considered in this equation. Its solution has a lorentzian form, consequently this equation characterizes a super diffusion like a L\'evy kind. In addition is obtained an equation that unifies the porous media and the logarithmic diffusion equations, including a generalized diffusion equation in fractal dimension. This unification is performed in the nonextensive thermostatistics context and increases the possibilities about the description of anomalous diffusive processes.Comment: 5 pages. To appear in Phys. Rev.

VERIFICA DI VITALITA' DEI BACILLI TUBERCOLARI IN PREPARATI MICROSCOPICI PER LA COLORAZIONE DI ZIEHL NEELSEN.

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Circulating extracellular particles from severe COVID-19 patients show altered profiling and innate lymphoid cell-modulating ability

Introduction: Extracellular vesicles (EVs) and particles (EPs) represent reliable biomarkers for disease detection. Their role in the inflammatory microenvironment of severe COVID-19 patients is not well determined. Here, we characterized the immunophenotype, the lipidomic cargo and the functional activity of circulating EPs from severe COVID-19 patients (Co-19-EPs) and healthy controls (HC-EPs) correlating the data with the clinical parameters including the partial pressure of oxygen to fraction of inspired oxygen ratio (PaO2/FiO2) and the sequential organ failure assessment (SOFA) score. Methods: Peripheral blood (PB) was collected from COVID-19 patients (n=10) and HC (n=10). EPs were purified from platelet-poor plasma by size exclusion chromatography (SEC) and ultrafiltration. Plasma cytokines and EPs were characterized by multiplex bead-based assay. Quantitative lipidomic profiling of EPs was performed by liquid chromatography/mass spectrometry combined with quadrupole time-of-flight (LC/MS Q-TOF). Innate lymphoid cells (ILC) were characterized by flow cytometry after co-cultures with HC-EPs or Co-19-EPs. Results: We observed that EPs from severe COVID-19 patients: 1) display an altered surface signature as assessed by multiplex protein analysis; 2) are characterized by distinct lipidomic profiling; 3) show correlations between lipidomic profiling and disease aggressiveness scores; 4) fail to dampen type 2 innate lymphoid cells (ILC2) cytokine secretion. As a consequence, ILC2 from severe COVID-19 patients show a more activated phenotype due to the presence of Co-19-EPs. Discussion: In summary, these data highlight that abnormal circulating EPs promote ILC2-driven inflammatory signals in severe COVID-19 patients and support further exploration to unravel the role of EPs (and EVs) in COVID-19 pathogenesis

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

© 2024 The Authors. Journal of Extracellular Vesicles, published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY), https://creativecommons.org/licenses/by/4.0/Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.Peer reviewe

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly

Indagine su alcuni fattori personali e interpersonali connessi alla paura del parto

I fattori sottostanti e le conseguenze della paura del parto (FOC) sono tuttora oggetto di studio. La FOC può portare alla richiesta di un parto cesareo e a sentimenti di insoddisfazione dopo la nascita (es. depressione materna, difficoltà col figlio). Presente in maniera differente nelle nullipare e nelle pluripare, è stata associata a caratteristiche personali della donna (ansia, bassa autostima, depressione), a fattori interpersonali (sostegno sociale, soddisfazione col partner), oltre che a life-event e fattori sociodemografici. Scopo del presente lavoro è stato indagare alcuni fattori sottostanti e conseguenze della FOC in un gruppo di donne italiane nullipare e pluripare utilizzando la versione italiana del Wijma Delivery Expectancy Questionnaire (W-DEQ A) tradotta dalla Prof. Saita (Univ. Cattolica). Questionari self report sono stati somministrati a 200 donne nel pre-parto (28°-30° settimana) e a un sottocampione randomizzato di 50 donne nel post parto (3 mesi); lo staff ostetrico ha fornito informazioni sul parto. Le aree di studio degli strumenti utilizzati sono: FOC e successivo vissuto; sintomatologia psicopatologica; qualità della relazione col partner e stile di attaccamento; sostegno sociale; stress percepito nella cura del figlio. Emergono alcuni fattori individuali e interpersonali maggiormente correlati alla FOC nel gruppo di donne italiane che in parte confermano la letteratura internazionale. Di particolare interesse sono i dati preliminari sul ruolo della percezione della qualità del legame col partner sulla FOC e sul successivo stress percepito nella cura del figlio nelle nullipare. La comprensione dei fattori connessi alla FOC costituisce un’area di studio recente in cui il ruolo del legame col partner è poco esplorat