Amelioration of cadmium-produced teratogenicity and genotoxicity in mice given Arthrospira maxima (Spirulina) treatment

Evaluation of the effects of Arthrospira maxima (AM) was made, otherwise known as Spirulina, on the teratogenicity, genotoxicity, and DNA oxidation processes induced by cadmium (Cd). Pregnant ICR mice were divided into groups and administered water, Cd only, AM only, or AM plus Cd. AM was administered orally at doses of 200, 400, and 800 mg/kg from gestational day 0 (GD0) to GD17, and at GD7 there was an intraperitoneal challenge of Cd (1.5 mg/kg). Cd only caused fetal malformations, including exencephaly, micrognathia, ablephary, microphthalmia, and clubfoot, as well as a significant increase in the quantity of micronucleated polychromatic erythrocytes (MNPE) and of micronucleated normochromatic erythrocytes (MNNE) in blood cells of both the mothers and their fetuses. An increased level of oxidation was also found, measured by a rise in the levels of the adduct 8-hydroxy-2-deoxyguanosine. In a dose-dependent manner, AM significantly reduced the number of external, visceral, and skeletal malformations, the quantity of MNPE and MNNE, and the level of DNA oxidation. The results suggest that AM may reduce the genotoxic effects and rates of congenital malformations caused by exposure to Cd in utero and that the antioxidant activity of this cyanobacterium could be responsible, at least in part, for producing this effect

Genotoxicity and oxidative stress induced by cadmium and zinc in the planarian, Dugesia dorotocephala

This study sought to determine the DNA damage, and the lipoperoxidative effect, as well as changes in the superoxide dismutase (SOD) and catalase (CAT) activities induced by CdSO4 (Cd), and ZnSO4 (Zn), in addition to two different mixtures of the metals. Planarian Dugesia dorotocephala collected in the Ignacio Ramirez reservoir, México, adapted to laboratory conditions, and exposed to the metals in a controlled system was used. Initially, LC50 at 96 h of exposure was determined and the result obtained were 0.69 mg/L for Cd, 11.99 mg/L for Zn, 10.28 mg/L for mix 1, and 8.11 mg/L for mix 2. Then, the comet assay showed a DNA damage increase induced by Cd (0.13 and 0.2 mg/L) as high as 94% over the control level; the effect by Zn (from 0.2 to 2.7 mg/L) was clearly lower, although statistically significant with the high concentrations tested. As regards the two mixtures, we observed a concentration dependent increase. Similarly, in respect to lipoperoxidation, we found a strong effect by Cd, a slight effect by Zn, and a concentration dependent effect induced by the mixtures. Finally, the activity of the tested enzymes was modified by the metals in relation to the concentration applied.Keywords: Zinc, cadmium, planarian, DNA damage, oxidative stressAfrican Journal of Biotechnology Vol. 12(25), pp. 4028-403

Antigenotoxic Effect of Chamomilla recutita (L.) Rauschert Essential Oil in Mouse Spermatogonial Cells, and Determination of Its Antioxidant Capacity in Vitro

Chamomilla recutita (L.) Rauschert (Asteraceae), popularly known as chamomile, is a plant used in traditional medicine for various therapeutic purposes. Chamomile essential oil (CEO) is particularly known to inhibit the genotoxic damage produced by mutagens in mice somatic cells. The aim of this research was to determine the inhibitory potential of CEO on the genotoxic damage produced by daunorubicin (DAU) in mice germ cells. We evaluated the effect of 5, 50, and 500 mg/kg of essential oil on the rate of sister chromatid exchange (SCE) induced in spermatogonia by 10 mg/kg of the mutagen. We found no genotoxicity of CEO, but detected an inhibition of SCE after the damage induced by DAU; from the lowest to the highest dose of CEO we found an inhibition of 47.5%, 61.9%, and 93.5%, respectively. As a possible mechanism of action, the antioxidant capacity of CEO was determined using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method and ferric thiocyanate assays. In the first test we observed a moderate scavenging potential of the oil; nevertheless, the second assay showed an antioxidant capacity similar to that observed with vitamin E. In conclusion, we found that CEO is an efficient chemoprotective agent against the damage induced by DAU in the precursor cells of the germinal line of mice, and that its antioxidant capacity may induce this effect

Evaluation of the anti-inflammatory capacity of beta-sitosterol in rodent assays

Background: Beta-sitosterol (BS) is a compound discovered to be present in numerous plants. A number of interesting biomedical properties have been attributed to BS, including immuno-modulating and anti-inflammatory activities. Therefore, the aim of this report was to evaluate its anti-inflammatory capacity by applying various rodent experimental tests.Methods. To carry out the objective of the study we applied the methods indicated here. Two of the adopted methods were based on the passive reverse Arthus reaction: the rat paw edema test and the rat pleurisy assay. We also applied two methods related with the non-specific acute inflammation: the mouse ear edema test, and the mouse mieloperoxidase activity assay.Results. The results obtained in all tests established a significant anti-inflammatory potential of BS. In the rat paw edema test we found an inhibitory effect which goes from 50-70%; in the rat pleurisy assay our findings with respect to the volume of pleural exuded showed a reduction of 46%, as well as a 20% low amount of neutrophils in comparison with the level of the control group. In the mouse ear edema test we found a mean inflammatory inhibition of 75%, and with respect to mieloproxidase activity the results showed a significant inhibition induced by the three doses of BS.Conclusions. In the present study we determined a potent anti-inflammatory capacity of BS in specific and nonspecific types of acute inflammation in rodents.Keywords: Beta-sitosterol, anti-inflammatory assays, mouse, ra

Investigation on the Protective Effect of α-Mannan against the DNA Damage Induced by Aflatoxin B1 in Mouse Hepatocytes

Aflatoxin B1 is a contaminant of agricultural and dairy products that can be related to mutagenic and carcinogenic effects. In this report we explore the capacity of α-mannan (Man) to reduce the DNA damage induced by AFB1 in mouse hepatocytes. For this purpose we applied the comet assay to groups of animals which were first administered Man (100, 400 and 700 mg/kg, respectively) and 20 min later 1.0 mg/kg of AFB1. Liver cells were obtained at 4, 10, and 16 h after the chemical administration and examined. The results showed no protection of the damage induced by AFB1 with the low dose of the polysaccharide, but they did reveal antigenotoxic activity exerted by the two high doses. In addition, we induced a co-crystallization between both compounds, determined their fusion points and analyzed the molecules by UV spectroscopy. The obtained data suggested the formation of a supramolecular complex between AFB1 and Man

Evaluación de la actividad antiinflamatoria, inmunológica y antioxidante de beta-sitosterol en modelos murinos / Evaluation of the anti-inflammatory, immunological and antioxidant activity of beta-sitosterol in murine models

Antecedentes: el beta-sitosterol (BS) es un compuesto presente en numerosas plantas. Se le han atribuido varias propiedades biomédicas interesantes del BS, incluidas actividades inmunomoduladoras y antiinflamatorias. Por lo tanto, el objetivo de este informe fue evaluar su capacidad antiinflamatoria mediante la aplicación de varias pruebas experimentales en roedores.  Métodos: Para llevar a cabo el objetivo del estudio se aplicaron los siguientes métodos. Dos métodos basados en la reacción pasiva inversa de Arthus: el ensayo de edema de pata de rata y el ensayo de pleuresía de rata, así como dos métodos relacionados con la inflamación aguda inespecífica: el ensayo de edema de oreja de ratón y el ensayo de actividad de mieloperoxidasa de ratón. Resultados: Los resultados obtenidos en todas las pruebas establecieron un importante potencial antiinflamatorio de BS. En la prueba de edema de pata de rata encontramos un efecto inhibidor que va del 50-70%; en el ensayo de pleuresía de rata nuestros hallazgos con respecto al volumen de exudado pleural mostraron una reducción del 46%, así como una cantidad baja de neutrófilos del 20% con respecto al nivel del grupo control. En el ensayo de edema de oreja de ratón encontramos una inhibición inflamatoria media del 75%, y respecto a la actividad mieloproxidasa los resultados mostraron una inhibición dependiente de la dosis inducida por BS. Conclusiones: En el presente estudio determinamos una potente capacidad antiinflamatoria de BS en tipos específicos y no específicos de inflamación aguda en roedores

AN ALTERNATIVE HEPATOPROTECTIVE AND ANTIOXIDANT AGENT: THE GERANIUM

The Geranium genus is taxonomically classified within the family Geraniaceae Juss, which includes 5-11 genera and nearly 750 species in total. The best-known genera of this family are Geranium, consisting largely of wild plants, and Pelargonium, consisting largely of ornamental plants. Traditional uses include as an antiseptic in wounds and as an antipyretic by infusion of the plant. Currently, eight different species of geraniums belonging to the family Geraniaceae have been identified in Hidalgo State in Central Mexico, and no chemical or pharmacological studies have been carried out in any of these eight species. All phytochemical studies on these species indicate the presence of polyphenolic compounds, including tannins, which are characterized as water-soluble compounds with molecular weights between 500 and 30,000 g/mol. These and other compounds warrant the exploration of the Germanium genus for uses related to ethanol-induced hepatotoxicity

Antigenotoxic Studies of Different Substances to Reduce the DNA Damage Induced by Aflatoxin B1 and Ochratoxin A

Mycotoxins are produced mainly by the mycelial structure of filamentous fungi, or more specifically, molds. These secondary metabolites are synthesized during the end of the exponential growth phase and appear to have no biochemical significance in fungal growth and development. The contamination of foods and feeds with mycotoxins is a significant problem for the adverse effects on humans, animals, and crops that result in illnesses and economic losses. The toxic effect of the ingestion of mycotoxins in humans and animals depends on a number of factors including intake levels, duration of exposure, toxin species, mechanisms of action, metabolism, and defense mechanisms. In general, the consumption of contaminated food and feed with mycotoxin induces to neurotoxic, immunosuppressive, teratogenic, mutagenic, and carcinogenic effect in humans and/or animals. The most significant mycotoxins in terms of public health and agronomic perspective include the aflatoxins, ochratoxin A (OTA), trichothecenes, fumonisins, patulin, and the ergot alkaloids. Due to the detrimental effects of these mycotoxins, several strategies have been developed in order to reduce the risk of exposure. These include the degradation, destruction, inactivation or removal of mycotoxins through chemical, physical and biological methods. However, the results obtained with these methods have not been optimal, because they may change the organoleptic characteristics and nutritional values of food. Another alternative strategy to prevent or reduce the toxic effects of mycotoxins is by applying antimutagenic agents. These substances act according to several extra- or intracellular mechanisms, their main goal being to avoid the interaction of mycotoxins with DNA; as a consequence of their action, these agents would inhibit mutagenesis and carcinogenesis. This article reviews the main strategies used to control AFB1 and ochratoxin A and contains an analysis of some antigenotoxic substances that reduce the DNA damage caused by these mycotoxins