Developing a food waste biorefinery: Lactic acid extraction using anionic resin and impacts on downstream biogas production

Coupling lactic acid (LA) production with food waste (FW) anaerobic digestion (AD) can facilitate the next generation biorefinery to increase revenue and economic viability of FW AD. For this, LA should be effectively extracted from complex fermentation broths with minimal adverse effects on subsequent AD to maximise economic benefit. This study evaluated LA recovery by adsorption using a polymeric resin (BA765), not previously tested for LA, to explore adsorption capacity and kinetics. Furthermore, biochemical methane potential (BMP) tests were utilised to assess the effect of LA extraction on subsequent AD by measuring biogas production from the solid and liquid extraction residues. Optimal adsorption conditions yielded a maximum capacity of 0.21 gLA·g−1resin from pure solutions at pH 2–4, which was insensitive to temperature. However, real mixed fermentation broth impurities reduced LA uptake by 37%. BMP tests showed that the solid and liquid extraction residues had significant methane potential, with only a 21% reduction in overall methane yield compared to the raw fermentation broth prior to LA extraction. LA production outweighed the loss in methane energy in terms of relative value and indicated a FW biorefinery concept could be commercially attractive and technically feasible

Lactic acid from mixed food wastes at a commercial biogas facility: Effect of feedstock and process conditions

Anaerobic digestion facilities can become biorefineries to produce higher-value products together with biogas energy and nutrient-rich digestate. To inform future biorefinery concepts with lactic acid recovery, the current study monitored organic acids in a pre-fermentation stage at a commercial anaerobic digestion facility. The study assessed lactic acid production performance and the impact of mixed food waste feedstocks and process conditions. Feed rate and feedstock composition varied weekly with waste availability. Normal operating conditions of the pre-fermentation stage included warm ambient conditions (24–35 °C), low pH (3.45 ± 0.03), a short hydraulic retention time (1–3.5 days) and stable organic loading rate (12 ± 2 kgVS.m−3.day−1). These conditions favoured lactic acid, being dominant at an encouraging average concentration of 21.70 g L−1, notably without any process optimisation or control. Lactobacillus constituted the majority of the microbial community in the pre-fermentation stage (98.1 %–99.1 % relative abundance) with an unknown Lactobacillus species and L. reuteri being the major species present. Grain processing waste and milk paste were positive influencers of LA concentration. The monitoring results, together with a simple economic evaluation, indicated that lactic acid recovery from a commercial food waste anaerobic digestion facility had baseline feasibility. In addition, there would be significant opportunities to increase economic performance by targeted process control and optimisation

Lactic acid from mixed food waste fermentation using an adapted inoculum: Influence of pH and temperature regulation on yield and product spectrum

Environmental conditions (pH and temperature) are expected to influence microbial community composition and product spectrum in mixed-culture food waste (FW) fermentation. However, some conditions may favour growth of multiple organisms that compete for common substrates or consume target metabolites. The inoculum plays an integral role in mixed-culture fermentation, but it is currently unknown how an adapted inoculum, known to selectively produce the target metabolite, would influence fermentation, and how environmental conditions could control fermentation outcomes. Therefore, this study assessed the effects of pH (uncontrolled vs. controlled pH 4.0–6.0) and temperature (35–60 ◦C) on lactic acid (LA) from synthetic mixed FW batch fermentation (80 gVS⋅L− 1) utilising an adapted fermentation inoculum known to produce significant LA (10% inoculum volume). Concentrations of LA and competing organic acids were measured. Uncontrolled pH encouraged Lactobacillus growth but resulted in a low LA yield due to inhibitory conditions. Controlled pH 6.0 improved LA production but introduced LA consumption and competitive butyrate production. Observed butyrate production was dependent on pH and temperature and correlated with the growth of Clostridium Sensu Stricto 12. At pH 6.0 and 50 ◦C, observable LA consumption was eliminated, and the LA yield was maximised at 0.55 gLA⋅gVS− 1 (39 gLA⋅L− 1) while Lactobacillus remained dominant. The adapted inoculum effectively promoted LA production, while pH and temperature regulation were effective control levers to target LA

Ammonia stress on a resilient mesophilic anaerobic inoculum: methane production, microbial community, and putative metabolic pathways

Short term inhibition tests, 16S rRNA tag sequencing and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt), were employed to visualise the effects of increasing total ammoniacal nitrogen (TAN) concentration (3400–10166 ppm TAN) on microbial community structure and metabolic pathways for acetate degradation. The rate of methane production on acetate was significantly reduced by TAN concentrations above 6133 ppm; however, methane continued to be produced, even at 10166 ppm TAN (0.026 ± 0.0003 gCOD.gVS−1inoculum.day−1). Hydrogenotrophic methanogenesis with syntrophic acetate oxidation (SAO) was identified as the dominant pathway for methane production. A shift towards SAO pathways at higher TAN concentrations and a decrease in the number of ‘gene hits’ for key genes in specific methanogenesis pathways was observed. Overall, the results highlighted potential for inhibition activity testing to be used together with PICRUSt, to estimate changes in microbial metabolism and to better understand microbial resilience in industrial AD facilities

Perceptions of species abundance, distribution, and diversity: Lessons from four decades of sampling on a government-managed reserve

We examined data relative to species abundance, distribution, and diversity patterns of reptiles and amphibians to determine how perceptions change over time and with level of sampling effort. Location data were compiled on more than one million individual captures or observations of 98 species during a 44-year study period on the US Department of Energy's (DOE) Savannah River Site Natichal Environmental Research Park (SRS-NERP) in South Carolina. We suggest that perceptions of herpetofaunal species diversity are strongly dependent on level of effort and that land management decisions based on short-term data bases for some faunal groups could result in serious errors in environmental management. We provide evidence that acquiring information on biodiversity distribution patterns is compatible with multiyear spatially extensive research programs and also provide a perspective of what might be achieved if long-term, coordinated research efforts were instituted nationwide. To conduct biotic surveys on government- managed lands, we recommend revisions in the methods used by government agencies to acquire and report biodiversity data. We suggest that government and industry employees engaged in biodiversity survey efforts develop proficiency in field identification for one or more major taxonomic groups and be encouraged to measure the status of populations quantitatively with consistent and reliable methodologies. We also suggest that widespread academic cooperation in the dissemination of information on regional patterns of biodiversity could result by establishment of a peer-reviewed, scientifically rigorous journal concerned with status and trends of the biota of the United States

The conservation status of the world’s reptiles

Effective and targeted conservation action requires detailed information about species, their distribution, systematics and ecology as well as the distribution of threat processes which affect them. Knowledge of reptilian diversity remains surprisingly disparate, and innovative means of gaining rapid insight into the status of reptiles are needed in order to highlight urgent conservation cases and inform environmental policy with appropriate biodiversity information in a timely manner. We present the first ever global analysis of extinction risk in reptiles, based on a random representative sample of 1500 species (16% of all currently known species). To our knowledge, our results provide the first analysis of the global conservation status and distribution patterns of reptiles and the threats affecting them, highlighting conservation priorities and knowledge gaps which need to be addressed urgently to ensure the continued survival of the world’s reptiles. Nearly one in five reptilian species are threatened with extinction, with another one in five species classed as Data Deficient. The proportion of threatened reptile species is highest in freshwater environments, tropical regions and on oceanic islands, while data deficiency was highest in tropical areas, such as Central Africa and Southeast Asia, and among fossorial reptiles. Our results emphasise the need for research attention to be focussed on tropical areas which are experiencing the most dramatic rates of habitat loss, on fossorial reptiles for which there is a chronic lack of data, and on certain taxa such as snakes for which extinction risk may currently be underestimated due to lack of population information. Conservation actions specifically need to mitigate the effects of human-induced habitat loss and harvesting, which are the predominant threats to reptiles