Managing native and non-native sea lamprey (Petromyzon marinus) through anthropogenic change: A prospective assessment of key threats and uncertainties.

Sea lamprey (Petromyzon marinus) is a species of conservation concern in their native range of the Atlantic coasts of Europe (Near Threatened to Critically Endangered) and North America (Secure to Critically Imperiled), and an invasive species of great economic and ecological concern in the Laurentian Great Lakes. Despite differences in life history strategy (anadromous natives vs adfluvial non-natives), the biology of sea lamprey is sufficiently similar to expect comparable responses to large-scale environmental change. We take a prospective look at the future (50 to 100 years) of sea lamprey management in an era of considerable environmental disturbance, and consider biological responses, management actions, and the future status of populations across the native and non-native ranges. Based on facilitated discussion by a diverse group of international experts, two major but poorly characterized classes of threats to sea lamprey were identified: climate change and socio-political issues. We discuss how climate induced changes affect growth, bioenergetics, and phenology of sea lamprey, and associated effects on control tactics (pesticides and barriers) and conservation. We consider tensions surrounding improving connectivity in the Great Lakes while controlling invasive sea lamprey, and discuss supplements and alternatives to pesticides and their wider effect, as well as the effects of new invasive species. To prevent the extirpation of native sea lamprey populations, or the re-expansion of non-native populations, we conclude with a call for new and ongoing dialogue and collaboration among all sea lamprey biologists and managers across the native and non-native range

MR-derived renal morphology and renal function in patients with atherosclerotic renovascular disease

Appropriate selection of patients with atherosclerotic renovascular disease (ARVD) for revascularization might be improved if accurate non-invasive investigations were used to assess severity of pre-existing parenchymal damage. The purpose of this study was to evaluate the associations between magnetic resonance imaging (MRI)-measured renal morphological parameters and single-kidney glomerular filtration rate (GFR) in ARVD. Three-dimensional (3D)-MRI was performed on 35 ARVD patients. Renal bipolar length (BL), parenchymal volume, parenchymal (PT), and cortical thicknesses (CT) were measured in 65 kidneys. Thirteen kidneys were supplied by normal vessels, 13 had insignificant (<50%) renal artery stenosis (RAS), 33 significant (≥50%) RAS, and six complete vessel occlusion. All patients underwent radioisotopic measurement of single-kidney GFR (isoSK-GFR). Overall, 3D parameters such as parenchymal volume were better correlates of isoSK-GFR (r=0.86, P<0.001) than BL (r=0.78, P<0.001), PT (r=0.63, P<0.001) or CT (r=0.60, P<0.001). Kidneys with ≥50% RAS did show significant reduction in mean CT compared to those supplied by normal vessel (5.67±1.63 vs 7.28±1.80 mm, P=0.002; 22.1% reduction) and an even greater loss of parenchymal volume (120.65±47.15 vs 179.24±86.90 ml, P<0.001; 32.7% reduction) with no significant reduction in BL. In a proportion of ≥50% RAS kidneys, a disproportionately high parenchymal volume to isoSK-GFR was observed supporting a concept of ‘hibernating parenchyma’. 3D parameters of parenchymal volume are stronger correlates of isoSK-GFR than two-dimensional measures of BL, PT or CT. 3D morphological evaluation together with isoSK-GFR might be useful in aiding patient selection for renal revascularization. Kidneys with increased parenchymal volume to SK-GFR might represent a subgroup with the potential to respond beneficially to angioplasty

Systematic event generator tuning for the LHC

In this article we describe Professor, a new program for tuning model parameters of Monte Carlo event generators to experimental data by parameterising the per-bin generator response to parameter variations and numerically optimising the parameterised behaviour. Simulated experimental analysis data is obtained using the Rivet analysis toolkit. This paper presents the Professor procedure and implementation, illustrated with the application of the method to tunes of the Pythia 6 event generator to data from the LEP/SLD and Tevatron experiments. These tunes are substantial improvements on existing standard choices, and are recommended as base tunes for LHC experiments, to be themselves systematically improved upon when early LHC data is available.Comment: 28 pages. Submitted to European Physical Journal C. Program sources and extra information are available from http://projects.hepforge.org/professor

Coordinated ras and rac activity shapes macropinocytic cups and enables phagocytosis of geometrically diverse bacteria

Engulfment of extracellular material by phagocytosis or macropinocytosis depends on the ability of cells to generate specialized cup-shaped protrusions. To effectively capture and internalize their targets, these cups are organized into a ring or ruffle of actin-driven protrusion encircling a non-protrusive interior domain. These functional domains depend on the combined activities of multiple Ras and Rho family small GTPases, but how their activities are integrated and differentially regulated over space and time is unknown. Here, we show that the amoeba Dictyostelium discoideum coordinates Ras and Rac activity using the multidomain protein RGBARG (RCC1, RhoGEF, BAR, and RasGAP-containing protein). We find RGBARG uses a tripartite mechanism of Ras, Rac, and phospholipid interactions to localize at the protruding edge and interface with the interior of both macropinocytic and phagocytic cups. There, we propose RGBARG shapes the protrusion by expanding Rac activation at the rim while suppressing expansion of the active Ras interior domain. Consequently, cells lacking RGBARG form enlarged, flat interior domains unable to generate large macropinosomes. During phagocytosis, we find that disruption of RGBARG causes a geometry-specific defect in engulfing rod-shaped bacteria and ellipsoidal beads. This demonstrates the importance of coordinating small GTPase activities during engulfment of more complex shapes and thus the full physiological range of microbes, and how this is achieved in a model professional phagocyte

Higher Order Evaluation of the Critical Temperature for Interacting Homogeneous Dilute Bose Gases

We use the nonperturbative linear \delta expansion method to evaluate analytically the coefficients c_1 and c_2^{\prime \prime} which appear in the expansion for the transition temperature for a dilute, homogeneous, three dimensional Bose gas given by T_c= T_0 \{1 + c_1 a n^{1/3} + [ c_2^{\prime} \ln(a n^{1/3}) +c_2^{\prime \prime} ] a^2 n^{2/3} + {\cal O} (a^3 n)\}, where T_0 is the result for an ideal gas, a is the s-wave scattering length and n is the number density. In a previous work the same method has been used to evaluate c_1 to order-\delta^2 with the result c_1= 3.06. Here, we push the calculation to the next two orders obtaining c_1=2.45 at order-\delta^3 and c_1=1.48 at order-\delta^4. Analysing the topology of the graphs involved we discuss how our results relate to other nonperturbative analytical methods such as the self-consistent resummation and the 1/N approximations. At the same orders we obtain c_2^{\prime\prime}=101.4, c_2^{\prime \prime}=98.2 and c_2^{\prime \prime}=82.9. Our analytical results seem to support the recent Monte Carlo estimates c_1=1.32 \pm 0.02 and c_2^{\prime \prime}= 75.7 \pm 0.4.Comment: 29 pages, 3 eps figures. Minor changes, one reference added. Version in press Physical Review A (2002

Asymptotically Improved Convergence of Optimized Perturbation Theory in the Bose-Einstein Condensation Problem

We investigate the convergence properties of optimized perturbation theory, or linear $\delta$ expansion (LDE), within the context of finite temperature phase transitions. Our results prove the reliability of these methods, recently employed in the determination of the critical temperature T_c for a system of weakly interacting homogeneous dilute Bose gas. We carry out the explicit LDE optimized calculations and also the infrared analysis of the relevant quantities involved in the determination of $T_c$ in the large-N limit, when the relevant effective static action describing the system is extended to O(N) symmetry. Then, using an efficient resummation method, we show how the LDE can exactly reproduce the known large-N result for $T_c$ already at the first non-trivial order. Next, we consider the finite N=2 case where, using similar resummation techniques, we improve the analytical results for the nonperturbative terms involved in the expression for the critical temperature allowing comparison with recent Monte Carlo estimates of them. To illustrate the method we have considered a simple geometric series showing how the procedure as a whole works consistently in a general case.Comment: 38 pages, 3 eps figures, Revtex4. Final version in press Phys. Rev.

A PI(3,5)P2 reporter reveals PIKfyve activity and dynamics on macropinosomes and phagosomes

Phosphoinositide signaling lipids (PIPs) are key regulators of membrane identity and trafficking. Of these, PI(3,5)P2 is one of the least well-understood, despite key roles in many endocytic pathways including phagocytosis and macropinocytosis. PI(3,5)P2 is generated by the phosphoinositide 5-kinase PIKfyve, which is critical for phagosomal digestion and antimicrobial activity. However PI(3,5)P2 dynamics and regulation remain unclear due to lack of reliable reporters. Using the amoeba Dictyostelium discoideum, we identify SnxA as a highly selective PI(3,5)P2-binding protein and characterize its use as a reporter for PI(3,5)P2 in both Dictyostelium and mammalian cells. Using GFP-SnxA, we demonstrate that Dictyostelium phagosomes and macropinosomes accumulate PI(3,5)P2 3 min after engulfment but are then retained differently, indicating pathway-specific regulation. We further find that PIKfyve recruitment and activity are separable and that PIKfyve activation stimulates its own dissociation. SnxA is therefore a new tool for reporting PI(3,5)P2 in live cells that reveals key mechanistic details of the role and regulation of PIKfyve/PI(3,5)P

Invasive species control: incorporating demographic data and seed dispersal into a management model for Rhododendron ponticum

Rhododendron ponticum is a serious invasive alien plant in the British Isles and is of significant conservation and economic concern. Here, we integrate information on both the life-history and spatial dynamics of this species within an individual-based, spatially-explicit model and investigate the effectiveness of different control strategies. Importantly, we simulate seed movement and dispersal using a mechanistic seed dispersal model. We investigate the effectiveness of initiating control at the edge versus the core of the infestation, with and without returning each year to remove seedlings. We compare these results to an age-dependent strategy whereby the oldest plants are removed each year. Age-dependent control, in which the oldest plants were removed first, was the most effective strategy investigated, both in terms of the probability of successful eradication and the number of years taken to control. We demonstrate that this is because the older (and taller) plants towards the core produce more seeds that, on average, travel further. Indeed, our results suggest that the expansion of the invading front is actually driven as much by seeds that disperse long distances from these larger plants as by the seed rain from recently matured plants located much closer to the front. Finally, we investigate the potential use of ‘quarantine lines’ - corridors of unsuitable habitat that are sufficiently wide to contain an infestation, preventing spread to vulnerable areas. This study has provided generic insights into best practice for management based on the current understanding of the biology and ecology of this pernicious, invasive plant

Testing the Gaussian expansion method in exactly solvable matrix models

The Gaussian expansion has been developed since early 80s as a powerful analytical method, which enables nonperturbative studies of various systems using `perturbative' calculations. Recently the method has been used to suggest that 4d space-time is generated dynamically in a matrix model formulation of superstring theory. Here we clarify the nature of the method by applying it to exactly solvable one-matrix models with various kinds of potential including the ones unbounded from below and of the double-well type. We also formulate a prescription to include a linear term in the Gaussian action in a way consistent with the loop expansion, and test it in some concrete examples. We discuss a case where we obtain two distinct plateaus in the parameter space of the Gaussian action, corresponding to different large-N solutions. This clarifies the situation encountered in the dynamical determination of the space-time dimensionality in the previous works.Comment: 30 pages, 15 figures, LaTeX; added references for section

Dependence of Variational Perturbation Expansions on Strong-Coupling Behavior. Inapplicability of delta-Expansion to Field Theory

We show that in applications of variational theory to quantum field theory it is essential to account for the correct Wegner exponent omega governing the approach to the strong-coupling, or scaling limit. Otherwise the procedure either does not converge at all or to the wrong limit. This invalidates all papers applying the so-called delta-expansion to quantum field theory.Comment: Author Information under http://www.physik.fu-berlin.de/~kleinert/institution.html . Latest update of paper (including all PS fonts) at http://www.physik.fu-berlin.de/~kleinert/34