Synthetic Morphology Using Alternative Inputs

Designing the shape and size of a cell is an interesting challenge for synthetic biology. Prolonged exposure to the mating pheromone α-factor induces an unusual morphology in yeast cells: multiple mating projections. The goal of this work was to reproduce the multiple projections phenotype in the absence of α-factor using a gain-of-function approach termed “Alternative Inputs (AIs)”. An alternative input is defined as any genetic manipulation that can activate the signaling pathway instead of the natural input. Interestingly, none of the alternative inputs were sufficient to produce multiple projections although some produced a single projection. Then, we extended our search by creating all combinations of alternative inputs and deletions that were summarized in an AIs-Deletions matrix. We found a genetic manipulation (AI-Ste5p ste2Δ) that enhanced the formation of multiple projections. Following up this lead, we demonstrated that AI-Ste4p and AI-Ste5p were sufficient to produce multiple projections when combined. Further, we showed that overexpression of a membrane-targeted form of Ste5p alone could also induce multiple projections. Thus, we successfully re-engineered the multiple projections mating morphology using alternative inputs without α-factor

Análise de incertezas físicas em simulação computacional de edificações residenciais

Resumo O objetivo deste trabalho é analisar incertezas físicas em simulação computacional de uma edificação residencial e sua implicação no desempenho térmico. O experimento foi elaborado com o método de Monte Carlo, que envolve as propriedades térmicas e físicas dos materiais da edificação. Foi analisada a sensibilidade das variáveis com coeficientes de regressão e correlação. Considerou-se o critério de graus-hora de desconforto por calor e por frio para o clima de Florianópolis, SC, conforme limites de conforto térmico adaptativo. Os resultados mostraram que as incertezas obtidas nos graus-hora são relativamente grandes, sendo de 32% no desconforto por frio e de 53% no desconforto por calor. Grande parte da incerteza é decorrente da temperatura média mensal do solo. Outras variáveis, como a absortância solar da cobertura e o calor específico da argamassa das paredes, resultam em impacto relevante no desconforto por calor, bem como a massa específica da argamassa e o calor específico da cerâmica das paredes no desconforto por frio. Essas variáveis precisam de maior precisão, seja por meio de bases de dados mais confiáveis, ou mesmo por medições em campo e/ou em laboratório

A Systematic Review of Three-Dimensional Printing in Liver Disease

The purpose of this review is to analyse current literature related to the clinical applications of 3D printed models in liver disease. A search of the literature was conducted to source studies from databases with the aim of determining the applications and feasibility of 3D printed models in liver disease. 3D printed model accuracy and costs associated with 3D printing, the ability to replicate anatomical structures and delineate important characteristics of hepatic tumours, and the potential for 3D printed liver models to guide surgical planning are analysed. Nineteen studies met the selection criteria for inclusion in the analysis. Seventeen of them were case reports and two were original studies. Quantitative assessment measuring the accuracy of 3D printed liver models was analysed in five studies with mean difference between 3D printed models and original source images ranging from 0.2 to 20%. Fifteen studies provided qualitative assessment with results showing the usefulness of 3D printed models when used as clinical tools in preoperative planning, simulation of surgical or interventional procedures, medical education, and training. The cost and time associated with 3D printed liver model production was reported in 11 studies, with costs ranging from US$13 to US$2000, duration of production up to 100 h. This systematic review shows that 3D printed liver models demonstrate hepatic anatomy and tumours with high accuracy. The models can assist with preoperative planning and may be used in the simulation of surgical procedures for the treatment of malignant hepatic tumours

Acidic Digestion in a Teleost: Postprandial and Circadian Pattern of Gastric pH, Pepsin Activity, and Pepsinogen and Proton Pump mRNAs Expression

Two different modes for regulation of stomach acid secretion have been described in vertebrates. Some species exhibit a continuous acid secretion maintaining a low gastric pH during fasting. Others, as some teleosts, maintain a neutral gastric pH during fasting while the hydrochloric acid is released only after the ingestion of a meal. Those different patterns seem to be closely related to specific feeding habits. However, our recent observations suggest that this acidification pattern could be modified by changes in daily feeding frequency and time schedule. The aim of this study was to advance in understanding the regulation mechanisms of stomach digestion and pattern of acid secretion in teleost fish. We have examined the postprandial pattern of gastric pH, pepsin activity, and mRNA expression for pepsinogen and proton pump in white seabream juveniles maintained under a light/dark 12/12 hours cycle and receiving only one morning meal. The pepsin activity was analyzed according to the standard protocol buffering at pH 2 and using the actual pH measured in the stomach. The results show how the enzyme precursor is permanently available while the hydrochloric acid, which activates the zymogen fraction, is secreted just after the ingestion of food. Results also reveal that analytical protocol at pH 2 notably overestimates true pepsin activity in fish stomach. The expression of the mRNA encoding pepsinogen and proton pump exhibited almost parallel patterns, with notable increases during the darkness period and sharp decreases just before the morning meal. These results indicate that white seabream uses the resting hours for recovering the mRNA stock that will be quickly used during the feeding process. Our data clearly shows that both daily illumination pattern and feeding time are involved at different level in the regulation of the secretion of digestive juices

Interaction of the Deubiquitinating Enzyme Ubp2 and the E3 Ligase Rsp5 Is Required for Transporter/Receptor Sorting in the Multivesicular Body Pathway

Protein ubiquitination is essential for many events linked to intracellular protein trafficking. We sought to elucidate the possible involvement of the S. cerevisiae deubiquitinating enzyme Ubp2 in transporter and receptor trafficking after we (this study) and others established that affinity purified Ubp2 interacts stably with the E3 ubiquitin ligase Rsp5 and the (ubiquitin associated) UBA domain containing protein Rup1. UBP2 interacts genetically with RSP5, while Rup1 facilitates the tethering of Ubp2 to Rsp5 via a PPPSY motif. Using the uracil permease Fur4 as a model reporter system, we establish a role for Ubp2 in membrane protein turnover. Similar to hypomorphic rsp5 alleles, cells deleted for UBP2 exhibited a temporal stabilization of Fur4 at the plasma membrane, indicative of perturbed protein trafficking. This defect was ubiquitin dependent, as a Fur4 N-terminal ubiquitin fusion construct bypassed the block and restored sorting in the mutant. Moreover, the defect was absent in conditions where recycling was absent, implicating Ubp2 in sorting at the multivesicular body. Taken together, our data suggest a previously overlooked role for Ubp2 as a positive regulator of Rsp5-mediated membrane protein trafficking subsequent to endocytosis

Classification of peanuts by wet chemical analysis, instrumental methods, electronic nose devices and sensory analysis

The peanut is the third most important oilseed crop in the world. Several species and types of wild and cultivated kinds of peanuts are known. Since they contain 50-55% oil and 25-28% protein, the potential for the development of an off-flavour during storage is significant (Ory et al., 1992; Bett et al, 1994). In routine analysis wer chemical methods are used for the determination of quality. These methods are always related to certain lipid parameters, for example the acid value is the amount of potassium hydroxide required to neutralise the fatty acids in 1g of fat, while the peroxide value is the quantity, which oxidises potassium under certain operating conditions. In addition, instrumental methods (e.g. gas chromatography) and sensory analysis are described as usable tools for the determination of the storage period, the quality and the origin. Apart from the time- consuming aspect of these methods, they are sensitive to misinterpretation. In the last ten years new devices, so called "electronic noses", have been described in the literature as a both cost-effective and timesaving substitute for these methods. Most of these chemical sensors showed deficiencies in selectivity, sensitivity und reproducibility (Haugen, 2001). The coupling of gas chromatography with an array of chemical sensors, the High Resolution Gas Chromatography-Selective Odorant Measurement by Multisensor Array (HRGC/SOMSA, Schieberle et al. 1997) represents the coupling of gas chromatography with an array of chemical sensors. This device has been used for the detection of odour-active compounds (Bücking and Steinhart, 2001). The aim of this paper is to compare these methods with regard to their utility for the classification of peanuts

A method for extracting performance metrics using work-order data

Holistic performance metrics are necessary to understand how operational resources are used and to detect anomalous zones, floors, equipment, and work-order categories in large commercial and institutional buildings. Work-order data in computerized maintenance management systems (CMMS) represent an untapped potential to extract such performance metrics. In this paper, a method to conduct text analytics on CMMS data is developed and demonstrated through a case study in which four years’ worth of data from four large commercial buildings are used. Association rule mining technique is employed to identify building, system, and component-level recurring work-order taxonomies and common failure modes. The results highlight the potential of kernel density functions, decision trees, Sankey diagrams, survival curves and stacked line plots to effectively visualize the temporal, spatial, and categorical anomalies in the complaint patterns. It is identified that often only a few floors and complaint types account for most of the complaints in a building. The analysis of operator comments reveal that the most frequent lighting-related complaints are resolved by replacing ballasts and lights, and the thermal and air quality complaints are addressed by adjusting the temperature setpoints, airflow rates, and fan operation schedules

Energy and comfort performance benefits of early detection of building sensor and actuator faults

This paper presents a building performance simulation-based investigation to better understand the energy and comfort performance benefits of early detection of common sensor and actuator faults. Five types of air-handling unit faults and four types of zone-level faults were implemented to the energy management system application of the building performance simulation tool EnergyPlus. During 50-year simulation periods, the faults were randomly permitted to affect 75 different components of an archetype medium-sized office building model. The sensitivity of the simulation results with respect to three variables was studied: fault recurrence period, fault repair period, and discomfort threshold for simulated complaints. The results indicate that the energy use intensity and the predicted percentage of dissatisfied exhibit a power–law relationship with time, in which most of the performance reductions occur in the first 10 years. If the work-orders are issued only upon occupant complaints, the faults were estimated to cause a 16–62% increase in the energy use intensity for heating, ventilation, and air-conditioning and a 11–38% increase in the predicted percentage of dissatisfied at the end of the 50-year simulation periods. The results indicate that if the faults can be detected within a month after their first appearance, almost all their detrimental effects on a building’s energy and comfort performance can be mitigated. Practical application: The methodology and results presented in this article are of practical use for those who study on-going commissioning, fault detection and diagnostics, and energy management systems in buildings. The simulation-based parametric analysis approach can be used to estimate the range of energy and comfort savings expected through early detection of common sensor and actuator faults in commercial buildings. Insights gathered from such an analysis can be used in planning the frequency of retro-commissioning and investments for automated fault detection and diagnostics systems