5,279 research outputs found
A study of environmental characterization of conventional and advanced aluminum alloys for selection and design. Phase 2: The breaking load test method
A technique is demonstrated for accelerated stress corrosion testing of high strength aluminum alloys. The method offers better precision and shorter exposure times than traditional pass fail procedures. The approach uses data from tension tests performed on replicate groups of smooth specimens after various lengths of exposure to static stress. The breaking strength measures degradation in the test specimen load carrying ability due to the environmental attack. Analysis of breaking load data by extreme value statistics enables the calculation of survival probabilities and a statistically defined threshold stress applicable to the specific test conditions. A fracture mechanics model is given which quantifies depth of attack in the stress corroded specimen by an effective flaw size calculated from the breaking stress and the material strength and fracture toughness properties. Comparisons are made with experimental results from three tempers of 7075 alloy plate tested by the breaking load method and by traditional tests of statistically loaded smooth tension bars and conventional precracked specimens
Vitamin D deficiency in myotonic dystrophy type 1
Myotonic dystrophy type 1 (DM1) is a multisystemic
disorder affecting, among others, the endocrine
system, with derangement of steroid hormones functions.
Vitamin D is a steroid recognized for its role in calcium
homeostasis. In addition, vitamin D influences muscle
metabolism by genomic and non-genomic actions,
including stimulation of the insulin-like-growth-factor 1
(IGF1), a major regulator of muscle trophism. To verify
the presence of vitamin D deficit in DM1 and its possible
consequences, serum 25-hydroxyvitamin D (25(OH)D),
calcium, parathormone (PTH), and IGF1 levels were
measured in 32 DM1 patients and in 32 age-matched
controls. Bone mineral density (BMD) and proximal
muscle strength were also measured by DXA and a
handheld dynamometer, respectively. In DM1 patients,
25(OH)D levels were reduced compared to controls, and a
significant decrease of IGF1 was also found. 25(OH)D
levels inversely correlated with CTG expansion size,
while IGF1 levels and muscle strength directly correlated
with levels of 25(OH)D lower than 20 and 10 ng/ml,
respectively. A significantly higher percentage of DM1
patients presented hyperparathyroidism as compared to
controls. Calcium levels and BMD were comparable
between the two groups. Oral administration of cholecalciferol
in 11 DM1 patients with severe vitamin D deficiency
induced a normal increase of circulating 25(OH)D,
ruling out defects in intestinal absorption or hepatic
hydroxylation. DM1 patients show a reduction of circulating
25(OH)D, which correlates with genotype and may
influence IGF1 levels and proximal muscle strength. Oral
supplementation with vitamin D should be considered in
DM1 and might mitigate muscle weakness
Effects of 5-fluorouracil on morphology, cell cycle, proliferation, apoptosis, autophagy and ros production in endothelial cells and cardiomyocytes
Antimetabolites are a class of effective anticancer drugs interfering in essential biochemical processes. 5-Fluorouracil (5-FU) and its prodrug Capecitabine are widely used in the treatment of several solid tumors (gastro-intestinal, gynecological, head and neck, breast carcinomas). Therapy with fluoropyrimidines is associated with a wide range of adverse effects, including diarrhea, dehydration, abdominal pain, nausea, stomatitis, and hand-foot syndrome. Among the 5-FU side effects, increasing attention is given to cardiovascular toxicities induced at different levels and intensities. Since the mechanisms related to 5-FU-induced cardiotoxicity are still unclear, we examined the effects of 5-FU on primary cell cultures of human cardiomyocytes and endothelial cells, which represent two key components of the cardiovascular system. We analyzed at the cellular and molecular level 5-FU effects on cell proliferation, cell cycle, survival and induction of apoptosis, in an experimental cardioncology approach. We observed autophagic features at the ultrastructural and molecular levels, in particular in 5-FU exposed cardiomyocytes. Reactive oxygen species (ROS) elevation characterized the endothelial response. These responses were prevented by a ROS scavenger. We found induction of a senescent phenotype on both cell types treated with 5-FU. In vivo, in a xenograft model of colon cancer, we showed that 5-FU treatment induced ultrastructural changes in the endothelium of various organs. Taken together, our data suggest that 5-FU can affect, both at the cellular and molecular levels, two key cell types of the cardiovascular system, potentially explaining some manifestations of 5-FU-induced cardiovascular toxicity
A New Limit on the Neutrinoless DBD of 130Te
We report the present results of CUORICINO a cryogenic experiment on
neutrinoless double beta decay (DBD) of 130Te consisting of an array of 62
crystals of TeO2 with a total active mass of 40.7 kg. The array is framed
inside of a dilution refrigerator, heavily shielded against environmental
radioactivity and high-energy neutrons, and operated at a temperature of ~8 mK
in the Gran Sasso Underground Laboratory. Temperature pulses induced by
particle interacting in the crystals are recorded and measured by means of
Neutron Transmutation Doped thermistors. The gain of each bolometer is
stabilized with voltage pulses developed by a high stability pulse generator
across heater resistors put in thermal contact with the absorber.
The calibration is performed by means of two thoriated wires routinely
inserted in the set-up. No evidence for a peak indicating neutrinoless DBD of
130Te is detected and a 90% C.L. lower limit of 1.8E24 years is set for the
lifetime of this process. Taking largely into account the uncertainties in the
theoretical values of nuclear matrix elements, this implies an upper boud on
the effective mass of the electron neutrino ranging from 0.2 to 1.1 eV. This
sensitivity is similar to those of the 76Ge experiments.Comment: 4 pages, 2 figure
Duchenne's muscular dystrophy involves a defective transsulfuration pathway activity
Duchenne muscular dystrophy (DMD) is the most frequent X chromosome-linked disease caused by mutations in the gene encoding for dystrophin, leading to progressive and unstoppable degeneration of skeletal muscle tissues. Despite recent advances in the understanding of the molecular processes involved in the pathogenesis of DMD, there is still no cure. In this study, we aim at investigating the potential involvement of the transsulfuration pathway (TSP), and its by-end product namely hydrogen sulfide (H2S), in primary human myoblasts isolated from DMD donors and skeletal muscles of dystrophic (mdx) mice. In myoblasts of DMD donors, we demonstrate that the expression of key genes regulating the H2S production and TSP activity, including cystathionine γ lyase (CSE), cystathionine beta-synthase (CBS), 3 mercaptopyruvate sulfurtransferase (3-MST), cysteine dioxygenase (CDO), cysteine sulfonic acid decarboxylase (CSAD), glutathione synthase (GS) and γ -glutamylcysteine synthetase (γ-GCS) is reduced. Starting from these findings, using Nuclear Magnetic Resonance (NMR) and quantitative Polymerase Chain Reaction (qPCR) we show that the levels of TSP-related metabolites such as methionine, glycine, glutathione, glutamate and taurine, as well as the expression levels of the aforementioned TSP related genes, are significantly reduced in skeletal muscles of mdx mice compared to healthy controls, at both an early (7 weeks) and overt (17 weeks) stage of the disease. Importantly, the treatment with sodium hydrosulfide (NaHS), a commonly used H2S donor, fully recovers the impaired locomotor activity in both 7 and 17 old mdx mice. This is an effect attributable to the reduced expression of pro-inflammatory markers and restoration of autophagy in skeletal muscle tissues. In conclusion, our study uncovers a defective TSP pathway activity in DMD and highlights the role of H2S-donors for novel and safe adjuvant therapy to treat symptoms of DMD
Double-beta decay of Te to the first 0 excited state of Xe with CUORICINO
The CUORICINO experiment was an array of 62 TeO single-crystal
bolometers with a total Te mass of kg. The experiment finished
in 2008 after more than 3 years of active operating time. Searches for both
and double-beta decay to the first excited state in
Xe were performed by studying different coincidence scenarios. The
analysis was based on data representing a total exposure of
N(Te)t=y. No evidence for a signal was
found. The resulting lower limits on the half lives are y (90% C.L.), and
y (90%
C.L.).Comment: 6 pages, 4 figure
Independent Ion Migration in Suspensions of Strongly Interacting Charged Colloidal Spheres
We report on sytematic measurements of the low frequency conductivity in
aequous supensions of highly charged colloidal spheres. System preparation in a
closed tubing system results in precisely controlled number densities between
1E16/m3 and 1E19/m^3 (packing fractions between 1E-7 and 1E-2) and electrolyte
concentrations between 1E-7 and 1E-3 mol/l. Due to long ranged Coulomb
repulsion some of the systems show a pronounced fluid or crystalline order.
Under deionized conditions we find s to depend linearily on the packing
fraction with no detectable influence of the phase transitions. Further at
constant packing fraction s increases sublinearily with increasing number of
dissociable surface groups N. As a function of c the conductivity shows
pronounced differences depending on the kind of electrolyte used. We propose a
simple yet powerful model based on independent migration of all species present
and additivity of the respective conductivity contributions. It takes account
of small ion macro-ion interactions in terms of an effectivly transported
charge. The model successfully describes our qualitatively complex experimental
observations. It further facilitates quantitative estimates of conductivity
over a wide range of particle and experimental parameters.Comment: 32 pages, 17 figures, 2 tables, Accepted by Physical Review
A Cryogenic Underground Observatory for Rare Events: Cuore, an Update
CUORE is a proposed tightly packed array of 1000 TeO_{2} bolometers, each
being a cube 5 cm on a side with a mass of 750 gms. The array consists of 25
vertical towers, arranged in a square, of 5 towers by 5 towers, each containing
10 layers of 4 crystals. The design of the detector is optimized for ultralow-
background searches for neutrinoless double beta decay of ^{130}Te (33.8%
abundance), cold dark matter, solar axions, and rare nuclear decays. A
preliminary experiment involving 20 crystals of various sizes (MIBETA) has been
completed, and a single CUORE tower is being constructed as a smaller scale
experiment called CUORICINO. The expected performance and sensitivity, based on
Monte Carlo simulations and extrapolations of present results, are reported.Comment: in press: Nucl. Phys. of Russian Academy of Sc
A Calorimetric Search on Double Beta Decay of 130Te
We report on the final results of a series of experiments on double decay of
130Te carried out with an array of twenty cryogenic detectors. The set-up is
made with crystals of TeO2 with a total mass of 6.8 kg, the largest operating
one for a cryogenic experiment. Four crystals are made with isotopically
enriched materials: two in 128Te and two others in 130Te. The remaining ones
are made with natural tellurium, which contains 31.7 % and 33.8 % 128Te and
130Te, respectively. The array was run under a heavy shield in the Gran Sasso
Underground Laboratory at a depth of about 3500 m.w.e. By recording the pulses
of each detector in anticoincidence with the others a lower limit of 2.1E23
years has been obtained at the 90 % C.L. on the lifetime for neutrinoless
double beta decay of 130Te. In terms of effective neutrino mass this is the
most restrictive limit in direct experiments, after those obtained with Ge
diodes. Limits on other lepton violating decays of 130Te and on the
neutrinoless double beta decay of 128Te to the ground state of 128Xe are also
reported and discussed. An indication is presented for the two neutrino double
beta decay of 130Te. Some consequences of the present results in the
interpretation of geochemical experiments are discussed.Comment: 14 pages, 3 figures, 2 tables; more analysis details. Accepted for
publication on Physics Letters
- …