Archaeological Excavation of the Priest Quarters, Mission San Francisco de la Espada, 41BX4, San Antonio, Texas

The following report is the result of two projects completed by the Center for Archaeological Research, of The University of Texas at San Antonio for San Francisco de la Espada/Catholic Diocese of San Antonio and J. T. Michel, Inc., under Texas Historical Commission Permit Number 2076. The investigations were conducted at Mission San Francisco de la Espada, San Antonio, Bexar County, Texas (41BX4). The initial investigation was conducted in November 1998, prior to the planned installation of electrical lines along the southwest corner of the Convento (complex of structures grouped around a patio area), while the additional excavations of July and August 1999, were conducted to coincide with restoration work being conducted on the Chapel and Priest Quarters. Additional investigations were also conducted in July and August 1999, along the southern-most walls of the Priest Quarters prior to the installation of new foundation piers and beams designed to stabilize the existing wall foundations. The excavations affected the exterior walls of the Convento; an area 1 m wide by 1 m deep, and 25.2 m long. The monitoring portion conducted in October and November 1999, focused on unexcavated areas along walls that were exposed during the stabilization work. During the course of the investigations exposed foundations and features were documented, with specific attention to mode of construction and condition. The excavations resulted in the recovery of a variety of Colonial and post-Colonial artifacts including ceramics, lithics, glass, metal, and animal bone. These excavations which supplied the opportunity for the recovery of valuable cultural data, also revealed noticeable differences in wall foundation construction and reconstruction, which strongly suggest at least two distinct construction sequences

Archaeological Testing for the Mission Road Realignment Project, Phase II, at Mission Concepcion, San Antonio, Texas

In July 1988, the Center for Archaeological Research (CAR) contracted with the city of San Antonio to perform archaeological testing for the Mission Road Realignment Project. This project was designated as Phase II since CAR performed previous archaeological testing during February 1987 (Labadie 1989). The Mission Road Realignment Project, Phase II proposed to relocate the position of Mission Road outside the line of the original west wall of Mission Concepcion. The testing sought to determine whether any structural remains or cultural deposits that may have been located outside the mission wall would be impacted by the proposed roadway. Archaeological testing with hand-excavated units and backhoe trenches established the location of the west wall of the mission quadrangle and a portion of an interior structure wall foundation with an associated hearth and cultural midden. The northwest corner of the mission is believed to be located under the current Mission Road. Mission-period pottery, metals tools, projectile points, and animal bone were recovered from the excavations

Investigations at the Vollrath Blacksmith Shop (41BX786), San Antonio, Bexar County, Texas

In May 1988, archival research was initiated to evaluate the archaeological potential of New City Block 102, Lots 10 through 15, in downtown San Antonio, Texas, proposed site of the new Bexar County Justice Center parking garage. The research indicated that the southern portion of Lots 10 and 11 would required further investigation at the 1874 site of Vollrath\u27s blacksmith shop. Field excavations were conducted in August 1988. This report discusses the archival research and the results of the field excavations

Simultaneous detection of Legionella species and L. anisa, L. bozemanii, L. longbeachae and L. micdadei using conserved primers and multiple probes in a multiplex real-time PCR assay

AbstractLegionnaires' disease is a severe respiratory disease that is estimated to cause between 8,000 and 18,000 hospitalizations each year, though the exact burden is unknown due to under-utilization of diagnostic testing. Although Legionella pneumophila is the most common species detected in clinical cases (80-90%), other species have also been reported to cause disease. However, little is known about Legionnaires' disease caused by these non-pneumophila species. We designed a multiplex real-time PCR assay for detection of all Legionella spp. and simultaneous specific identification of four clinically-relevant Legionella species, L. anisa, L. bozemanii, L. longbeachae, and L. micdadei, using 5′-hydrolysis probe real-time PCR. The analytical sensitivity for detection of nucleic acid from each target species was ≤50fg per reaction. We demonstrated the utility of this assay in spiked human sputum specimens. This assay could serve as a tool for understanding the scope and impact of non-pneumophila Legionella species in human disease

Dependence on RAD52 and RAD1 for anticancer drug resistance mediated by inactivation of mismatch repair genes

AbstractMismatch repair (MMR) proteins repair mispaired DNA bases and have an important role in maintaining the integrity of the genome [1]. Loss of MMR has been correlated with resistance to a variety of DNA-damaging agents, including many anticancer drugs [2]. How loss of MMR leads to resistance is not understood, but is proposed to be due to loss of futile MMR activity and/or replication stalling [3,4]. We report that inactivation of MMR genes (MLH1, MLH2, MSH2, MSH3, MSH6, but not PMS1) in isogenic strains of Saccharomyces cerevisiae led to increased resistance to the anticancer drugs cisplatin, carboplatin and doxorubicin, but had no effect on sensitivity to ultraviolet C (UVC) radiation. Sensitivity to cisplatin and doxorubicin was increased in mlh1 mutant strains when the MLH1 gene was reintroduced, demonstrating a direct involvement of MMR proteins in sensitivity to these DNA-damaging agents. Inactivation of MLH1, MLH2 or MSH2 had no significant effect, however, on drug sensitivities in the rad52 or rad1 mutant strains that are defective in mitotic recombination and removing unpaired DNA single strands. We propose a model whereby MMR proteins – in addition to their role in DNA-damage recognition – decrease adduct tolerance during DNA replication by modulating the levels of recombination-dependent bypass. This hypothesis is supported by the finding that, in human ovarian tumour cells, loss of hMLH1 correlated with acquisition of cisplatin resistance and increased cisplatin-induced sister chromatid exchange, both of which were reversed by restoration of hMLH1 expression

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Update on Prevention, Diagnosis, and Treatment of Chronic Hepatitis B: AASLD 2018 Hepatitis B Guidance

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Sedentary time in older men and women: an international consensus statement and research priorities

Sedentary time is a modifiable determinant of poor health, and in older adults, reducing sedentary time may be an important first step in adopting and maintaining a more active lifestyle. The primary purpose of this consensus statement is to provide an integrated perspective on current knowledge and expert opinion pertaining to sedentary behaviour in older adults on the topics of measurement, associations with health outcomes, and interventions. A secondary yet equally important purpose is to suggest priorities for future research and knowledge translation based on gaps identified. A five-step Delphi consensus process was used. Experts in the area of sedentary behaviour and older adults (n=15) participated in three surveys, an in-person consensus meeting, and a validation process. The surveys specifically probed measurement, health outcomes, interventions, and research priorities. The meeting was informed by a literature review and conference symposium, and it was used to create statements on each of the areas addressed in this document. Knowledge users (n=3) also participated in the consensus meeting. Statements were then sent to the experts for validation. It was agreed that self-report tools need to be developed for understanding the context in which sedentary time is accumulated. For health outcomes, it was agreed that the focus of sedentary time research in older adults needs to include geriatric-relevant health outcomes, that there is insufficient evidence to quantify the dose-response relationship, that there is a lack of evidence on sedentary time from older adults in assisted facilities, and that evidence on the association between sedentary time and sleep is lacking. For interventions, research is needed to assess the impact that reducing sedentary time, or breaking up prolonged bouts of sedentary time has on geriatric-relevant health outcomes. Research priorities listed for each of these areas should be considered by researchers and funding agencies

Manual / Issue 5 / Unfinished

Manual, a journal about art and its making. Unfinished.The fifth issue. Loose threads unknotted. Ideas unrealized. Outlines left bare. Function unperformed. Patterns uncut. Luster removed with time and wear. We rarely examine unfinished things. The unfinished is easily overlooked in favor of the fully rendered and complete, but consider those sketchy lines, those fraying ends: the unfinished has potency. The unfinished offers evidence of process, reveals traces of technique, trembles with latent possibility. The essays, images, and projects presented in the fifth issue of Manual attend to the fluid potential of objects that are in some way incomplete. Softcover, 68 pages. Published 2015 by the RISD Museum. Manual 5 (Unfinished) contributors include Jen Bervin, Jean Blackburn, Gina Borromeo, Laurie Brewer, A. Will Brown, Bolaji Campbell, Dennis Congdon, Jeremy Deller, Jan Howard, Kate Irvin, Maureen C. O’Brien, Emily J. Peters, Siebren Versteeg, Elizabeth A. Williams, and C. D. Wright.https://digitalcommons.risd.edu/risdmuseum_journals/1004/thumbnail.jp

HLA-B*35-Px–mediated acceleration of HIV-1 infection by increased inhibitory immunoregulatory impulses

A subset of HLA-B*35 alleles, B*35-Px, are strongly associated with accelerated HIV-1 disease progression for reasons that are not understood. Interestingly, the alternative set of B*35 subtypes, B*35-PY, have no detectable impact on HIV-1 disease outcomes, even though they can present identical HIV-1 epitopes as B*35-Px molecules. Thus, the differential impact of these alleles on HIV-1 disease progression may be unrelated to interactions with HIV-1–specific CD8+ T cells. Here, we show that the B*35-Px molecule B*3503 binds with greater affinity to immunoglobulin-like transcript 4 (ILT4), an inhibitory MHC class I receptor expressed on dendritic cells, than does the B*35-PY molecule B*3501, even though these two B*35 molecules differ by only one amino acid and present identical HIV-1 epitopes. The preferential recognition of B*3503 by ILT4 was associated with significantly stronger dendritic cell dysfunction in in vitro functional assays. Moreover, HIV-1–infected carriers of B*3503 had poor dendritic cell functional properties in ex vivo assessments when compared with carriers of the B*3501 allele. Differential interactions between HLA class I allele subtypes and immunoregulatory MHC class I receptors on dendritic cells thus provide a novel perspective for the understanding of MHC class I associations with HIV-1 disease progression and for the manipulation of host immunity against HIV-1