Cytochrome b divergence between avian sister species is linked to generation length and body mass

It is increasingly realised that the molecular clock does not tick at a constant rate. Rather, mitochondrial mutation rates are influenced by factors such as generation length and body mass. This has implications for the use of genetic data in species delimitation. It could be that speciation, as recognised by avian taxonomists, is associated with a certain minimum genetic distance between sister taxa, in which case we would predict no difference in the cytochrome b divergence of sister taxa according to the species’ body size or generation time. Alternatively, if what taxonomists recognise as speciation has tended to be associated with the passage of a minimum amount of time since divergence, then there might be less genetic divergence between sister taxa with slower mutation rates, namely those that are heavier and/or with longer generation times. After excluding non-flying species, we analysed a database of over 600 avian sister species pairs, and found that species pairs with longer generation lengths (which tend to be the larger species) showed less cytochrome b divergence. This finding cautions against using any simple unitary criterion of genetic divergence to delimit species

Using the unique spectral signature of guano to identify unknown seabird colonies

Despite the threats faced by seabirds in both terrestrial and marine habitats, even basic knowledge of the locations of colonies, population sizes and trends is lacking for many remote areas of the world. Recent studies have shown that the guano of Adélie penguins can be identified from Landsat Enhanced Thematic Mapper (ETM) imagery and used to map colonies on coasts around continental Antarctica. Our study highlights a new technique based on the unique spectral signature of guano that can be used to discriminate seabird colonies from background geology and vegetation in a wider range of natural environments, including the vegetated and zoologically-diverse region of the Antarctic Peninsula; moreover, the method was effective for all densely colonial, surface-nesting seabirds. Using Landsat ETM imagery, we correctly identified all known seabird colonies of over 50 pairs in the area of Marguerite Bay. Almost all other areas with a similar spectral signature that were outside known breeding areas were single pixels that were readily distinguishable from genuine colonies. If these were excluded, only 4.1% of pixels appeared to represent unknown breeding or roosting sites, and warrant further investigation. The spatial extent of the guano provided a general guide to the number of individuals present, but further work would be required to determine the accuracy of this method for estimating population size. Spectral profiles of guano collected by satellite and hand-held spectrometers were compared with available data in spectral libraries and did not match with any known geological profile. There may also be potential for discriminating colonies of different species that differ in phenology and show seasonal changes in diet by the carefully-timed acquisition of suitable satellite imagery. We conclude that the remotely-sensed guano signature is a good indicator of the location of seabird breeding or roosting sites, with potentially wide application to other areas of the world

High-throughput, automated quantification of white matter neurons in mild malformation of cortical development in epilepsy

Introduction In epilepsy, the diagnosis of mild Malformation of Cortical Development type II (mMCD II) predominantly relies on the histopathological assessment of heterotopic neurons in the white matter. The exact diagnostic criteria for mMCD II are still ill-defined, mainly because findings from previous studies were contradictory due to small sample size, and the use of different stains and quantitative systems. Advance in technology leading to the development of whole slide imaging with high-throughput, automated quantitative analysis (WSA) may overcome these differences, and may provide objective, rapid, and reliable quantitation of white matter neurons in epilepsy. This study quantified the density of NeuN immunopositive neurons in the white matter of up to 142 epilepsy and control cases using WSA. Quantitative data from WSA was compared to two other systems, semi-automated quantitation, and the widely accepted method of stereology, to assess the reliability and quality of results from WSA. Results All quantitative systems showed a higher density of white matter neurons in epilepsy cases compared to controls (P = 0.002). We found that, in particular, WSA with user-defined region of interest (manual) was superior in terms of larger sampled size, ease of use, time consumption, and accuracy in region selection and cell recognition compared to other methods. Using results from WSA manual, we proposed a threshold value for the classification of mMCD II, where 78% of patients now classified with mMCD II were seizure-free at the second post-operatively follow up. Conclusion This study confirms the potential role of WSA in future quantitative diagnostic histology, especially for the histopathological diagnosis of mMCD

Effect of the unpolarized spin state in spin-correlation measurement of two protons produced in the 12C(d,2He) reaction

In this note we discuss the effect of the unpolarized state in the spin-correlation measurement of the $^1S_0$ two-proton state produced in 12C(d,2He) reaction at the KVI, Groningen. We show that in the presence of the unpolarized state the maximal violation of the CHSH-Bell inequality is lower than the classical limit if the purity of the state is less than $\sim \verb+70$. In particular, for the KVI experiment the violation of the CHSH-Bell inequality should be corrected by a factor $\sim\verb+10$ from the pure $^1S_0$ state.Comment: 6 pages, to appear in J. Phys.

Astronaut EVA Exposure Estimates from CAD Model Spacesuit Geometry

Ongoing assembly and maintenance activities at the International Space Station (ISS) require much more extravehicular activity (EVA) than did the earlier U.S. Space Shuttle missions. It is thus desirable to determine and analyze, and possibly foresee, as accurately as possible what radiation exposures crew members involved in EVAs will experience in order to minimize risks and to establish exposure limits that must not to be exceeded. A detailed CAD model of the U.S. Space Shuttle EVA Spacesuit, developed at NASA Langley Research Center (LaRC), is used to represent the directional shielding of an astronaut; it has detailed helmet and backpack structures, hard upper torso, and multilayer space suit fabric material. The NASA Computerized Anatomical Male and Female (CAM and CAF) models are used in conjunction with the space suit CAD model for dose evaluation within the human body. The particle environments are taken from the orbit-averaged NASA AP8 and AE8 models at solar cycle maxima and minima. The transport of energetic particles through space suit materials and body tissue is calculated by using the NASA LaRC HZETRN code for hadrons and a recently developed deterministic transport code, ELTRN, for electrons. The doses within the CAM and CAF models are determined from energy deposition at given target points along 968 directional rays convergent on the points and are evaluated for several points on the skin and within the body. Dosimetric quantities include contributions from primary protons, light ions, and electrons, as well as from secondary brehmsstrahlung and target fragments. Directional dose patterns are displayed as rays and on spherical surfaces by the use of a color relative intensity representation

Survival of a long-lived single island endemic, the Raso lark Alauda razae, in relation to age, fluctuating population and rainfall.

Estimating and understanding variation in survival rates is crucial for the management of threatened species, especially those with limited population sizes and/or restricted ranges. Using a capture-resighting dataset covering 2004-2017, we estimate adult survival in the Raso lark Alauda razae, a Critically Endangered single-island Cape Verdean endemic, whose population varied 25-fold during the study. Average annual adult survival was similar for males (0.813 ± 0.011) and females (0.826 ± 0.011) over the period. These values are high for a temperate passerine but not unusual for an insular tropical species like the lark. The oldest bird was recorded 13 years after first ringing. There was strong evidence that survival varied among years (between 0.57 and 0.95), being generally higher in wetter years. Survival, especially of males, was lower when the population was large, but only in drier years. Survival declined with age but there was no evidence that this decline was other than linear. High survival, even in the face of dry conditions, at least when the population is depressed, has probably contributed to the persistence of the species on its 7 km2 island home over several centuries

Young starless cores embedded in the magnetically dominated Pipe Nebula

The Pipe Nebula is a massive, nearby dark molecular cloud with a low star-formation efficiency which makes it a good laboratory to study the very early stages of the star formation process. The Pipe Nebula is largely filamentary, and appears to be threaded by a uniform magnetic field at scales of few parsecs, perpendicular to its main axis. The field is only locally perturbed in a few regions, such as the only active cluster forming core B59. The aim of this study is to investigate primordial conditions in low-mass pre-stellar cores and how they relate to the local magnetic field in the cloud. We used the IRAM 30-m telescope to carry out a continuum and molecular survey at 3 and 1 mm of early- and late-time molecules toward four selected starless cores inside the Pipe Nebula. We found that the dust continuum emission maps trace better the densest regions than previous 2MASS extinction maps, while 2MASS extinction maps trace better the diffuse gas. The properties of the cores derived from dust emission show average radii of ~0.09 pc, densities of ~1.3x10^5 cm^-3, and core masses of ~2.5 M_sun. Our results confirm that the Pipe Nebula starless cores studied are in a very early evolutionary stage, and present a very young chemistry with different properties that allow us to propose an evolutionary sequence. All of the cores present early-time molecular emission, with CS detections toward all the sample. Two of them, Cores 40 and 109, present strong late-time molecular emission. There seems to be a correlation between the chemical evolutionary stage of the cores and the local magnetic properties that suggests that the evolution of the cores is ruled by a local competition between the magnetic energy and other mechanisms, such as turbulence.Comment: Accepted for publication in ApJ. 15 pages, 5 figures, 9 table

Polarization Correlations of 1S0 Proton Pairs as Tests of Bell and Wigner Inequalities

In an experiment designed to overcome the loophole of observer dependent reality and satisfying the counterfactuality condition, we measured polarization correlations of 1S0 proton pairs produced in 12C(d,2He) and 1H(d,He) reactions in one setting. The results of these measurements are used to test the Bell and Wigner inequalties against the predictions of quantum mechanics.Comment: 8 pages, 4 figure

Survival of a long-lived single island endemic, the Raso lark Alauda razae, in relation to age, fluctuating population and rainfall

Abstract: Estimating and understanding variation in survival rates is crucial for the management of threatened species, especially those with limited population sizes and/or restricted ranges. Using a capture-resighting dataset covering 2004–2017, we estimate adult survival in the Raso lark Alauda razae, a Critically Endangered single-island Cape Verdean endemic, whose population varied 25-fold during the study. Average annual adult survival was similar for males (0.813 ± 0.011) and females (0.826 ± 0.011) over the period. These values are high for a temperate passerine but not unusual for an insular tropical species like the lark. The oldest bird was recorded 13 years after first ringing. There was strong evidence that survival varied among years (between 0.57 and 0.95), being generally higher in wetter years. Survival, especially of males, was lower when the population was large, but only in drier years. Survival declined with age but there was no evidence that this decline was other than linear. High survival, even in the face of dry conditions, at least when the population is depressed, has probably contributed to the persistence of the species on its 7 km2 island home over several centuries