111 research outputs found
Election proximity and representation focus in party-constrained environments
Do elected representatives have a time-constant representation focus or do they adapt their focus depending on election proximity? In this article, we examine these overlooked theoretical and empirical puzzles by looking at how reelection-seeking actors adapt their legislative behavior according to the electoral cycle. In parliamentary democracies, representatives need to serve two competing principals: their party and their district. Our analysis hinges on how representatives make a strategic use of parliamentary written questions in a highly party-constrained institutional context to heighten their reselection and reelection prospects. Using an original data set of over 32,000 parliamentary questions tabled by Portuguese representatives from 2005 to 2015, we examine how time interacts with two key explanatory elements: electoral vulnerability and party size. Results show that representation focus is not static over time and, in addition, that electoral vulnerability and party size shape strategic use of parliamentary questions
Home Range Use and Movement Patterns of Non-Native Feral Goats in a Tropical Island Montane Dry Landscape
Advances in wildlife telemetry and remote sensing technology facilitate studies of broad-scale movements of ungulates in relation to phenological shifts in vegetation. In tropical island dry landscapes, home range use and movements of non-native feral goats (Capra hircus) are largely unknown, yet this information is important to help guide the conservation and restoration of some of the world’s most critically endangered ecosystems. We hypothesized that feral goats would respond to resource pulses in vegetation by traveling to areas of recent green-up. To address this hypothesis, we fitted six male and seven female feral goats with Global Positioning System (GPS) collars equipped with an Argos satellite upload link to examine goat movements in relation to the plant phenology using the Normalized Difference Vegetation Index (NDVI). Movement patterns of 50% of males and 40% of females suggested conditional movement between non-overlapping home ranges throughout the year. A shift in NDVI values corresponded with movement between primary and secondary ranges of goats that exhibited long-distance movement, suggesting that vegetation phenology as captured by NDVI is a good indicator of the habitat and movement patterns of feral goats in tropical island dry landscapes. In the context of conservation and restoration of tropical island landscapes, the results of our study identify how non-native feral goats use resources across a broad landscape to sustain their populations and facilitate invasion of native plant communities
Transcriptome sequencing of three Pseudo-nitzschia species reveals comparable gene sets and the presence of Nitric Oxide Synthase genes in diatoms
Diatoms are among the most diverse eukaryotic microorganisms on Earth, they are responsible for a large fraction of primary production in the oceans and can be found in different habitats. Pseudo-nitzschia are marine planktonic diatoms responsible for blooms in coastal and oceanic waters. We analyzed the transcriptome of three species, Pseudo-nitzschia arenysensis, Pseudo-nitzschia delicatissima and Pseudo-nitzschia multistriata, with different levels of genetic relatedness. These species have a worldwide distribution and the last one produces the neurotoxin domoic acid. We were able to annotate about 80% of the sequences in each transcriptome and the analysis of the relative functional annotations allowed comparison of the main metabolic pathways, pathways involved in the biosynthesis of isoprenoids (MAV and MEP pathways), and pathways putatively involved in domoic acid synthesis. The search for homologous transcripts among the target species and other congeneric species resulted in the discovery of a sequence annotated as Nitric Oxide Synthase (NOS), found uniquely in Pseudo-nitzschia multistriata. The predicted protein product contained all the domains of the canonical metazoan sequence. Putative NOS sequences were found in other available diatom datasets, supporting a role for nitric oxide as signaling molecule in this group of microalgae
Genic and Global Functions for Paf1C in Chromatin Modification and Gene Expression in Arabidopsis
In budding yeast, intragenic histone modification is linked with transcriptional elongation through the conserved regulator Paf1C. To investigate Paf1C-related function in higher eukaryotes, we analyzed the effects of loss of Paf1C on histone H3 density and patterns of H3 methylated at K4, K27, and K36 in Arabidopsis genes, and integrated this with existing gene expression data. Loss of Paf1C did not change global abundance of H3K4me3 or H3K36me2 within chromatin, but instead led to a 3′ shift in the distribution of H3K4me3 and a 5′ shift in the distribution of H3K36me2 within genes. We found that genes regulated by plant Paf1C showed strong enrichment for both H3K4me3 and H3K27me3 and also showed a high degree of tissue-specific expression. At the Paf1C- and PcG-regulated gene FLC, transcriptional silencing and loss of H3K4me3 and H3K36me2 were accompanied by expansion of H3K27me3 into the promoter and transcriptional start regions and further enrichment of H3K27me3 within the transcribed region. These results highlight both genic and global functions for plant Paf1C in histone modification and gene expression, and link transcriptional activity with cellular memory
Genome-Wide Transcriptome Analyses of Silicon Metabolism in Phaeodactylum tricornutum Reveal the Multilevel Regulation of Silicic Acid Transporters
BACKGROUND:Diatoms are largely responsible for production of biogenic silica in the global ocean. However, in surface seawater, Si(OH)(4) can be a major limiting factor for diatom productivity. Analyzing at the global scale the genes networks involved in Si transport and metabolism is critical in order to elucidate Si biomineralization, and to understand diatoms contribution to biogeochemical cycles. METHODOLOGY/PRINCIPAL FINDINGS:Using whole genome expression analyses we evaluated the transcriptional response to Si availability for the model species Phaeodactylum tricornutum. Among the differentially regulated genes we found genes involved in glutamine-nitrogen pathways, encoding putative extracellular matrix components, or involved in iron regulation. Some of these compounds may be good candidates for intracellular intermediates involved in silicic acid storage and/or intracellular transport, which are very important processes that remain mysterious in diatoms. Expression analyses and localization studies gave the first picture of the spatial distribution of a silicic acid transporter in a diatom model species, and support the existence of transcriptional and post-transcriptional regulations. CONCLUSIONS/SIGNIFICANCE:Our global analyses revealed that about one fourth of the differentially expressed genes are organized in clusters, underlying a possible evolution of P. tricornutum genome, and perhaps other pennate diatoms, toward a better optimization of its response to variable environmental stimuli. High fitness and adaptation of diatoms to various Si levels in marine environments might arise in part by global regulations from gene (expression level) to genomic (organization in clusters, dosage compensation by gene duplication), and by post-transcriptional regulation and spatial distribution of SIT proteins
Special cases : moons, rings, comets, trojans
Non-planetary bodies provide valuable insight into our current under-
standing of planetary formation and evolution. Although these objects are
challeng- ing to detect and characterize, the potential information to be drawn
from them has motivated various searches through a number of techniques. Here,
we briefly review the current status in the search of moons, rings, comets, and
trojans in exoplanet systems and suggest what future discoveries may occur in
the near future.Comment: Invited review (status August 2017
JADES: Probing interstellar medium conditions at z ∼ 5.5-9.5 with ultra-deep JWST/NIRSpec spectroscopy
We present emission-line ratios from a sample of 27 Lyman-break galaxies from z∼ 5.5-9.5 with-17.0 5.5 spectra. We find that the emission-line ratios exhibited by these z∼ 5.5-9.5 galaxies occupy clearly distinct regions of line-ratio space compared to typical z∼ 0-3 galaxies, instead being more consistent with extreme populations of lower-redshift galaxies. This is best illustrated by the [O III]/[O II] ratio, tracing interstellar medium (ISM) ionisation, in which we observe more than half of our sample to have [O III]/[O II] > 10. Our high signal-to-noise spectra reveal more than an order of magnitude of scatter in line ratios such as [O II]/Hβ and [O III]/[O II], indicating significant diversity in the ISM conditions within the sample. We find no convincing detections of [N II] λ6583 in our sample, either in individual galaxies, or a stack of all G395M/F290LP spectra. The emission-line ratios observed in our sample are generally consistent with galaxies with extremely high ionisation parameters (log U∼-1.5), and a range of metallicities spanning from ∼0.1 × Z⊙ to higher than ∼0.3 × Z⊙, suggesting we are probing low-metallicity systems undergoing periods of rapid star formation, driving strong radiation fields. These results highlight the value of deep observations in constraining the properties of individual galaxies, and hence probing diversity within galaxy population
The influence of surface type on the absorbed radiation by a human under hot, dry conditions
JADES: Discovery of extremely high equivalent width Lyman- α emission from a faint galaxy within an ionized bubble at z = 7.3
We report the discovery of a remarkable Lyα emitting galaxy at z = 7.2782, JADES-GS+53.16746-27.7720 (shortened to JADES-GS-z7-LA), with rest-frame equivalent width, EW0(Lyα) = 388.0 ± 88.8 Å and UV magnitude -17.0. The spectroscopic redshift is confirmed via rest-frame optical lines [O- ¯II], Hβ and [O- ¯III] in its JWST/NIRSpec Micro-Shutter Assembly (MSA) spectrum. The Lyα line is detected in both lower resolution (R ∼ 100) PRISM as well as medium resolution (R ∼ 1000) G140M grating spectra. The line spread function-deconvolved Lyα full width at half maximum in the grating is 383.9 ± 56.2 km s-1 and the Lyα velocity offset compared to the systemic redshift is 113.3 ± 80.0 km s-1, indicative of very little neutral gas or dust within the galaxy. We estimate the Lyα escape fraction to be > 70%. JADES-GS-z7-LA has a [O- ¯III]/[O- ¯II] ratio (O32) of 11.1 ± 2.2 and a ([O- ¯III] + [O- ¯II])/Hβ ratio (R23) of 11.2 ± 2.6, consistent with low metallicity and high ionization parameters. Deep NIRCam imaging also revealed a close companion source (separated by 0.23- ³), which exhibits similar photometry to that of JADES-GS-z7-LA, with a photometric excess in the F410M NIRCam image consistent with [O- ¯III] + Hβ emission at the same redshift. The spectral energy distribution of JADES-GS-z7-LA indicates a bursty star formation history, with a low stellar mass of 107† M·. Assuming that the Lyα transmission through the intergalactic medium is the same as its measured escape fraction, an ionized region of size > 1.5 pMpc is needed to explain the high Lyα EW and low velocity offset compared to systemic seen in JADES-GS-z7-LA. Owing to its UV-faintness, we show that it is incapable of single-handedly ionizing a region large enough to explain its Lyα emission. Therefore, we suggest that JADES-GS-z7-LA (and possibly the companion source) may be a part of a larger overdensity, presenting direct evidence of overlapping ionized bubbles at z > 7
JADES NIRSpec Spectroscopy of GN-z11: Lyman- α emission and possible enhanced nitrogen abundance in a z = 10.60 luminous galaxy
We present JADES JWST/NIRSpec spectroscopy of GN-z11, the most luminous candidate z>10 Lyman break galaxy in the GOODS-North field with MUV =-21.5. We derive a redshift of z = 10.603 (lower than previous determinations) based on multiple emission lines in our low and medium resolution spectra over 0.7- 5.3 μm. We significantly detect the continuum and measure a blue rest-UV spectral slope of β =-2.4. Remarkably, we see spatially extended Lyman-α in emission (despite the highly neutral intergalactic medium expected at this early epoch), offset 555 km s-1 redwards of the systemic redshift. From our measurements of collisionally excited lines of both low and high ionisation (including [O II] λ3727, [Ne III] λ3869, and C III] λ1909), we infer a high ionisation parameter (log U ∼-2). We detect the rarely seen N IV] λ1486 and N III] λ1748 lines in both our low and medium resolution spectra, with other high ionisation lines seen in the low resolution spectrum, such as He II (blended with O III]) and C IV (with a possible P-Cygni profile). Based on the observed rest-UV line ratios, we cannot conclusively rule out photoionisation from an active galactic nucleus (AGN), although the high C III]/He II and N III]/He II ratios are compatible with a star formation explanation. If the observed emission lines are powered by star formation, then the strong N III] λ1748 observed may imply an unusually high N/O abundance. Balmer emission lines (Hγ, Hδ) are also detected, and if powered by star formation rather than an AGN, we infer a star formation rate of ∼20-30 M⊙ yr-1 (depending on the initial mass function) and low dust attenuation. Our NIRSpec spectroscopy confirms that GN-z11 is a remarkable galaxy with extreme properties seen 430 Myr after the Big Bang
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