An Extended Culture System that Supports Human Primordial Germ Cell-like Cell Survival and Initiation of DNA Methylation Erasure.

The development of an in vitro system in which human primordial germ cell-like cells (hPGCLCs) are generated from human pluripotent stem cells (hPSCs) has been invaluable to further our understanding of human primordial germ cell (hPGC) specification. However, the means to evaluate the next fundamental steps in germ cell development have not been well established. In this study we describe a two dimensional extended culture system that promotes proliferation of specified hPGCLCs, without reversion to a pluripotent state. We demonstrate that hPGCLCs in extended culture undergo partial epigenetic reprogramming, mirroring events described in hPGCs in vivo, including a genome-wide reduction in DNA methylation and maintenance of depleted H3K9me2. This extended culture system provides a new approach for expanding the number of hPGCLCs for downstream technologies, including transplantation, molecular screening, or possibly the differentiation of hPGCLCs into gametes by in vitro gametogenesis

Patterns of Land Use Change, Land Governance, and the Supply of Ecosystem Services in a Multifunctional Landscape: A Case Study from Upstate SC, USA

Urban expansion and development continue to increase globally, threatening rural multifunctional landscapes that provide a diversity of direct and indirect ecological benefits. However, not all landscapes are equally impacted, thus making conservation planning more difficult. We used 8 years of land use and land cover to understand this change in Greenville Co. SC, a region experiencing rapid expansion of development into rural landscapes. Regression models helped explain the relationships between development predictor variables and rural response variables. To explore where these changes were occurring, we determined which size categories of land parcels were the most developed and how this is changing over time. Developed open space was revealed to significantly impact rural character, pasture, and evergreen forest most frequently. It was also found to be most prevalent in the smallest parcels. Determining relationships between development, rural character, and multifunctionality will aid in potential solutions for the conservation of multifunctional landscapes

The Grizzly, September 26, 2024

New Member Education Begins • October is Lantern Submission Month • Yik Yak Culture • Madeline Hepburn is Missing: A Short Story by Ze\u27ev Shaheen • Love of the Craft • Opinions: PSA: Flush the Toilet! • Women\u27s Soccer Soars to Great Start! • Men\u27s Soccer Kicks Off With Success! • Performance of the Weekhttps://digitalcommons.ursinus.edu/grizzlynews/2037/thumbnail.jp

Dallas Bower: a producer for television's early years, 1936-39

Having worked in the film industry as a sound technician and then director, Dallas Bower (1907-99) was appointed in 1936 as one of two senior producers at the start of the BBC Television service. Over the next three years Bower produced as well as directed many ground-breaking live programmes, including the opening-day broadcast on 2 November 1936; the BBC Television Demonstration Film (1937, his only surviving pre-war production); a modern-dress Julius Caesar (1938), in uniforms suggestive of a Fascist disctatorship; Act II of Tristan and Isolde (1938); Patrick Hamilton’s play Rope (1939), utilising extended single camera-shots camera-shots; numerous ballets, among them Checkmate (1938); and ambitious outside broadcasts from the film studios at Denham and Pinewood. Developing the working practices of producing for the theatre, film industry and radio, Bower was a key figure in defining the role of the creative television producer at the start of the medium. Among his innovations, according to his unpublished autobiographical fragment ‘Playback’ (written 1995), was the introduction of a drawn studio plan for the four cameras employed in all live broadcasts from Alexandra Palace. Using Bower’s writings (among them his 1936 book Plan for Cinema), his BECTU History Project interview, the BBC Written Archives and contemporary industry coverage, this article reconstructs the early development of the role of staff television producer in order to consider the questions of autonomy, agency and institutional constraints at the BBC in the pre-war years

A hemilabile NHC-gold complex and its application to the redox neutral 1,2-oxyarylation of feedstock alkenes.

We describe a Au(I) complex modified with a hemi-labile (C^N) N-heterocyclic carbene ligand that is able to mediate oxidative addition of aryl iodides. Detailed computational and experimental investigations have been undertaken to verify and rationalize the oxidative addition process. Application of this initiation mode has resulted in the first examples of "exogenous oxidant-free" AuI/AuIII catalyzed 1,2-oxyarylations of ethylene and propylene. These demanding yet powerful processes establish these commodity chemicals as nucleophilic-electrophilic building blocks in catalytic reaction design

Multiplexed characterization of rationally designed promoter architectures deconstructs combinatorial logic for IPTG-inducible systems

A crucial step towards engineering biological systems is the ability to precisely tune the genetic response to environmental stimuli. In the case of Escherichia coli inducible promoters, our incomplete understanding of the relationship between sequence composition and gene expression hinders our ability to predictably control transcriptional responses. Here, we profile the expression dynamics of 8269 rationally designed, IPTG-inducible promoters that collectively explore the individual and combinatorial effects of RNA polymerase and LacI repressor binding site strengths. We then fit a statistical mechanics model to measured expression that accurately models gene expression and reveals properties of theoretically optimal inducible promoters. Furthermore, we characterize three alternative promoter architectures and show that repositioning binding sites within promoters influences the types of combinatorial effects observed between promoter elements. In total, this approach enables us to deconstruct relationships between inducible promoter elements and discover practical insights for engineering inducible promoters with desirable characteristics

The Vehicle, Spring 1984

Vol. 25, No. 2 Table of Contents The OpeningKathy Fordpage 3 PaintThomas B. Waltrippage 3 PhotographLisa Fresepage 4 City as Castigating AnimalJerry McAnultypage 5 LimitsMichelle Mitchellpage 6 In SilenceKeila Tooleypage 6 PhotographLisa Fresepage 7 Indian CreekJerry McAnultypage 8 The Right Idea about RainMichelle Mitchellpage 8 Van Gogh\u27s Young Male PeasantKeila Tooleypage 9 AbigailMaggie Kennedypage 9 CleaningJennifer K. Soulepage 10 Heat WaveJerry McAnultypage 11 VigilJanet Gracepage 11 Hatred of ClownsMichelle Mitchellpage 12 Trees in the YardKeila Tooleypage 13 Lost on Back RoadsToby Mirabellipage 13 The Death of My Father\u27s HandsGina Grillopage 14 Soft LandingMaggie Kennedypage 14 Incident in the Generic AisleMaggie Kennedypage 15 Happy Well-adjusted Individuals, ConsideringJennifer K. Soulepage 16 Old ManGina Grillopage 17 It Doesn\u27t MatterTed Boonepage 18 A Love Poem for Charles DonaldCathy Moepage 18 David Willey at Whitman WharfSue Oiler Millerpage 19 I Am the ArtsBrenda Renee Drummerpage 20 Mothers and DaughtersJennifer K. Soulepage 22 ScratchesMichelle Mitchellpage 26 SedativeStacey Flanniganpage 26 Calloused HandJanet Gracepage 27 Cool CatStacey Flanniganpage 27 For My History Teacher, Miss WilhelmCathy Moepage 28 Short Poem for Chris\u27s EyesBob Zordanipage 28 MissingMichelle Mitchellpage 29 Over ThereGraham Lewispage 30 Strange BedfellowsMaggie Kennedypage 31 Retired PlumberMichelle Mitchellpage 32 Rumors of War DeadKathy Fordpage 33 Night LightsKeila Tooleypage 33 Fish on the Train TracksJerry McAnultypage 34 DaddyKeily Tooleypage 34 A Message to the MessengerAmy Eadespage 35 Dancing AloneKathy Bowerpage 36 HandsMaggie Kennedypage 36 The Key to the Ladies\u27s RoomMichelle Mitchellpage 37 Crashing the BlueStacey Flanniganpage 37 The Cradle will FallMichelle Mitchellpate 38 One IslandKeila Tooleypage 38 Black Walnut PaintSue Oiler Millerpage 39 SerendipityGraham Lewispage 40 Cover ArtLisa Fresehttps://thekeep.eiu.edu/vehicle/1045/thumbnail.jp

Neural Models of Normal and Abnormal Behavior: What Do Schizophrenia, Parkinsonism, Attention Deficit Disorder, and Depression Have in Common?

Defense Advanced Research Projects Agency and Office of Naval Research (N00014-95-1-0409); National Science Foundation (IRI-97-20333

Caribou, Petroleum, and the Limits of Locality in the Canada–US Borderlands

his article discusses Karsten Heuer’s 2006 book Being Caribou in light of debates in ecocriticism and border studies about how to define the local in the context of environmental problems of vast range and uncertain temporality. It explores how Heuer’s book about following the Porcupine Caribou herd’s migration engages in multiple forms of boundary crossing—between countries, between hemispheric locations, and between species—and shows how insights from Indigenous storytelling complicate the book’s appeal to environmentalist readers by asserting a prior, transnational Indigenous presence in the transboundary landscapes of present-day Alaska and the Yukon

Patient-specific cancer genes contribute to recurrently perturbed pathways and establish therapeutic vulnerabilities in esophageal adenocarcinoma

Abstract: The identification of cancer-promoting genetic alterations is challenging particularly in highly unstable and heterogeneous cancers, such as esophageal adenocarcinoma (EAC). Here we describe a machine learning algorithm to identify cancer genes in individual patients considering all types of damaging alterations simultaneously. Analysing 261 EACs from the OCCAMS Consortium, we discover helper genes that, alongside well-known drivers, promote cancer. We confirm the robustness of our approach in 107 additional EACs. Unlike recurrent alterations of known drivers, these cancer helper genes are rare or patient-specific. However, they converge towards perturbations of well-known cancer processes. Recurrence of the same process perturbations, rather than individual genes, divides EACs into six clusters differing in their molecular and clinical features. Experimentally mimicking the alterations of predicted helper genes in cancer and pre-cancer cells validates their contribution to disease progression, while reverting their alterations reveals EAC acquired dependencies that can be exploited in therapy