Comparaison de différentes techniques de greffage vis-à-vis de leur efficacité de transmission virale sur vigne

Dans le but d'effectuer un criblage rapide d'un grand nombre de variétes de vigne vis-à-vis de leur résistance aux virus, nous avons recherché quelle méthode d'inoculation était la plus adaptée . L'inoculation mécanique d'un virus à la vigne étant difficile à obtenir, nous avons comparé différentes techniques de greffage: greffage sur bois, greffage en vert avec porte-greffe préalablement enraciné ou non, chaque fois, deux positions possibles pour l'inoculum: greffon ou porte-greffe.Les résultats de cette étude indiquent que le greffage sur bois avec l'inoculum en position de porte-greffe correspond à la méthode la plus efficace. Toutefois, le greffage en vert avec porte-greffe non enraciné donne également de bons résultats, toujours avec l'inoculum en position de porte-greffe. Etant donnés les avantages que présente la greffe en vert en ce qui concerne la surface necessaire à sa réalisation et sa vitesse de réalisation, ainsi que son utilisation possible tout au long de l'année, elle paraît tout à fait adaptée pour inoculer un grand nombre de variétés. Comparison of the viral transmission efficiency of different grafting techniques on grapevinesWith the objective of screening a great number of grapevine varieties for their resistance to virus diseases, we looked for the most appropriate technique of inoculation. As the mechanical inoculation of a virus to the grapevine succeeds only under special conditions, we compared different grafting techniques: dormant grafting and green grafting, the last with and without previously rooted rootstock. For each of these three methods, the inoculum was used as the stock on one hand, as the scion on the other hand. This study proved that the dormant grafting technique using the inoculum as the rootstock is the most efficient technique. Nevertheless, good results were obtained by using the green grafting technique without previously rooting the rootstock, the inoculum being also used as the rootstock. As the green grafting method doesn't require a great area, is not time-consuming and can be performed throughout the year, it seems therefore to be a very convenient method of inoculating a great number of varieties

Molecular outflows in the young open cluster IC348

We present a wide-field survey of the young open cluster IC348 for molecular H2 outflows. Outflow activity is only found at its south-western limit, where a new subcluster of embedded sources is in an early phase of its formation. If the IC348 cluster had been built up by such subclusters forming at different times, this could explain the large age-spread that Herbig (1998) found for the IC348 member stars. In addition to several compact groups of H2 knots, our survey reveals a large north-south oriented outflow, and we identify the newly discovered far-infrared and mm-object IC348MMS as its source. New deep images in the 1-0 S(1) line of molecular hydrogen trace the HH211 jet and counterjet as highly-collimated chains of knots, resembling the interferometric CO and SiO jets. This jet system appears rotated counter-clockwise by about 3 degrees with respect to the prominent H2 bow shocks. Furthermore, we resolve HH211-mm as a double point-like source in the mm-continuum.Comment: 10 pages, 9 figures, accepted for publication in Ap

Cooling Strategies for Greenhouses in Summer: Control of Fogging by Pulse Width Modulation

The possibilities for improving the control of greenhouse fogging systems, were studied by comparing several combinations of ventilation cooling techniques, shade screening and low-pressure fogging. The study was divided into three parts: experiments, modelling and simulations. In the first part of the paper, ten combinations of five cooling techniques were tested during the summers of 2002 and 2003 in a 132m2 greenhouse with a steel structure and a single-layer methacrylate cover located in Madrid, Spain. An analysis of variance of the climatic parameters was carried out to determine which combinations produced significant differences in inside temperature or relative humidity. Comparing the values for the inside to outside temperature difference, the combination of a shade screen and above-screen fogging achieved a difference in temperature almost the same as that for under-screen fogging, but the relative humidity was significantly lower. In the second part of the study a dynamic model was developed (2002) and validated (2003). The mean absolute error obtained for inside temperature was similar in the fit and the validation and it was less than 1.5 1C in both cases. The model was used to simulate the inside air temperature for a fog system working without shading, and above and under a shade screen. Control algorithms were developed for reducing system water consumption. In the three cases a simple on/off control with a fixed fogging cycle was compared with a pulse width modulation (PWM) strategy, in which the duration of the fogging pulse was increased as a function of inside temperature. The strategies with PWM applied to the fog system were able to reduce water consumption by 8–15% with respect to the strategies with a fixed fogging cycle

Reliability of 2D ultrasound imaging associated with transient ShearWave Elastography method to analyze spastic gastrocnemius medialis muscle architecture and viscoelastic properties

PurposeThe aim of the study was to assess the reliability of pennation angle (PA) and muscle thickness (MT) 2D measurements and of shear elastic modulus measurement, using ultrasound imaging (US). Those measurements were made on spastic gastrocnemius medialis muscle at rest and at maximal passive stretching, in post-stroke hemiplegic patients. The paretic side measurements were compared to non-paretic side.Material and methodsFourteen patients took part in 2 inter-session reliability experiments, realized at a 7 days interval by the same operator. The Aixplorer® Supersonic US scanner with the transient ShearWave Elastography (SWE) software was used. The stretching experiments were made manually and controlled by a goniometer.ResultsThe reliability of the 2D measurements was good. The coefficient of variation (CV) was 6.30% for MT measurement at rest, 6.40% and 8.26% for PA at rest and at maximal passive stretching respectively. The reliability of the shear elastic modulus measurement in the sagittal plane was good only at rest with a CV of 9.86%, versus 40.58% at stretching. None of the shear elastic modulus measurements in the axial plane were good. At rest, MT and PA were weaker on the paretic side (14.25±3.12mm and 17.32±5.10°) versus non-paretic side (16.30±3.19mm and 21.08±5.05°) (P<0.0001 and P=0.006). At rest, there was a small difference in the shear elastic modulus between the paretic side and the non-paretic side (5.40±1.67kPa versus 6.20±2.18kPa, P=0.041).DiscussionThis is the first description of muscle spastic structure using SWE with Supersonic Shear Imaging. 2D US associated with SWE shows promise in terms of muscular atrophy quantification and muscle histological quality assessment. These structural properties reflect some of the functional abilities regardless of motor control. It should enable further research on therapies, which impact muscle tissue quality, such as botulinum neurotoxin injections

NOD × 129.H2g7 Backcross Delineates 129S1/SvImJ-Derived Genomic Regions Modulating Type 1 Diabetes Development in Mice

OBJECTIVE: Introduction of genes targeted in 129/Sv embryonic stem (ES) cells into NOD mice brings about linked genes that may modulate type 1 diabetes. Our objective was to identify 129S1/SvJ non-MHC regions contributing type 1 diabetes resistance or susceptibility in backcross to NOD/LtJ. RESEARCH DESIGN AND METHODS: After congenic transfer of the NOD H2(g7) haplotype onto 129S1/Sv, 310 females were produced by NOD x (NOD x 129.H2(g7))F1 backcross (N2). A genome scan for quantitative trait locus (QTL) affecting clinical diabetes, age of diabetes onset, and insulitis severity was performed using subphenotype characteristics to improve power and resolution for detection of diabetes susceptibility loci. RESULTS: Thirty-six of 310 (11.6%) N2 females developed type 1 diabetes between 14 and 40 weeks. Significant evidence of linkage for only a single previously reported Idd complex locus (Idd10/17/18, chromosome [Chr] 3) was indicated for clinical diabetes. The quantitative traits of insulitis either alone or combined with age at type 1 diabetes onset were significantly linked to known Idd regions on Chr 1 (Idd5 region), Chr 4 (Idd9 region), Chr 8 (Idd22), Chr 11 (Idd4.3), and proximal Chr 17 (Idd16 region). Significant 129S1/Sv resistance contributions were identified on Chr 1, 15 (two loci), and 19, with suggestive evidence for additional novel 129/Sv resistance QTL on Chr 5 and 17 and susceptibility on Chr 2. CONCLUSIONS: The 129S1/SvJ genome harbors collections of both known and potentially novel non-MHC Idd loci. Investigators targeting 129/Sv genes mapping within chromosomal regions reported herein or elsewhere in the genome need to exclude potential contributions from linked Idd loci by generating a NOD.129 control strain expressing the nontargeted allele

Protocole de fabrication par voie colloïdalede cristaux photoniques 3D

session 7 " Cristaux photoniques "National audienceNous présentons ici les détails d'un processus sol-gel utilisé pour synthétiser des sphères de silice, en portant une attention particulière aux conditions expérimentales permettant de contrôler leur taille. Nous avons élaboré un protocole dans le but d'obtenir des microsphères avec une faible dispersion, et démontré que de larges domaines cristallins ordonnés d'opale synthétique montrant une " stop band " peuvent être produits en quelques jours par déposition verticale et sédimentation assistée par évaporation. La microscopie électronique à balayage a été employée pour caractériser les échantillons. Des mesures de réflexion et transmission ont été effectuées pour mettre en évidence la haute qualité des opales réalisées

International multicentre observational study to assess the efficacy and safety of a 0·5 mg kg−1 per day starting dose of oral corticosteroids to treat bullous pemphigoid

BackgroundEuropean guidelines propose a 0 center dot 5 mg kg(-1) per day dose of oral prednisone as initial treatment for bullous pemphigoid (BP). We assessed the safety and efficacy of this regimen depending on BP extent and general condition of the patients.MethodsIn a prospective international study, we consecutively included all patients diagnosed with BP. Patients received a 0 center dot 5 mg kg(-1) per day dose of prednisone, which was then gradually tapered 15 days after disease control, with the aim of stopping prednisone or maintaining minimal treatment (0 center dot 1 mg kg(-1) per day) within 6 months after the start of treatment. The two coprimary endpoints were control of disease activity at day 21 and 1-year overall survival. Disease severity was assessed according to the Bullous Pemphigoid Disease Area Index (BPDAI) score.ResultsIn total, 198 patients were included between 2015 and 2017. The final analysis comprised 190 patients with a mean age of 80 center dot 9 (SD 9 center dot 1) years. Control of disease activity was achieved at day 21 in 119 patients [62 center dot 6%, 95% confidence interval (CI) 55 center dot 3-69.5]; 18 of 24 patients (75%, 95% CI 53 center dot 3-90 center dot 2), 75 of 110 patients (68 center dot 8%, 95% CI 59 center dot 2-77 center dot 3) and 26 of 56 patients (46.4%, 95% CI 33 center dot 0-60 center dot 3) had mild, moderate and severe BP, respectively (P = 0 center dot 0218). A total of 30 patients died during the study. The overall Kaplan-Meier 1-year survival was 82 center dot 6% (95% CI 76 center dot 3-87 center dot 4) corresponding to 90 center dot 9%, 83 center dot 0% and 80 center dot 0% rates in patients with mild, moderate and severe BP, respectively (P = 0 center dot 5). Thresholds of 49 points for BPDAI score and 70 points for Karnofsky score yielded maximal Youden index values with respect to disease control at day 21 and 1-year survival, respectively.ConclusionsA 0 center dot 5 mg kg(-1) per day dose of prednisone is a valuable therapeutic option in patients with mild or moderate BP whose general condition allows them to be autonomous.</p

A Study of the Luminosity and Mass Functions of the Young IC 348 Cluster using FLAMINGOS Wide-Field Near-Infrared Images

We present wide-field near-infrared (JHK) images of the young, 2 Myr IC 348 cluster taken with FLAMINGOS. We use these new data to construct an infrared census of sources, which is sensitive enough to detect a 10 Mjup brown dwarf seen through an extinction of Av=7mag. We examine the cluster's structure and relationship to the molecular cloud and construct the cluster's K band luminosity function. Using our model luminosity function algorithm, we derive the cluster's initial mass function throughout the stellar and substellar regimes and find that the IC 348 IMF is very similar to that found for the Trapezium Cluster with both cluster IMFs having a mode between 0.2 - 0.08 Msun. In particular we find that, similar to our results for the Trapezium, brown dwarfs constitute only 1 in 4 of the sources in the IC 348 cluster. We show that a modest secondary peak forms in the substellar IC 348 KLF, corresponding to the same mass range responsible for a similar KLF peak found in the Trapezium. We interpret this KLF peak as either evidence for a corresponding secondary IMF peak at the deuterium burning limit, or as arising from a feature in the substellar mass-luminosity relation that is not predicted by current theoretical models. Lastly, we find that IC 348 displays radial variations of its sub-solar (0.5 - 0.08 Msun) IMF on a parsec scale. Whatever mechanism that is breaking the universality of the IMF on small spatial scales in IC 348 does not appear to be acting upon the brown dwarf population, whose relative size does not vary with distance from the cluster center.Comment: 53 pages, 20 figures, AASTeX5.0. Color version of Figure 1 made available in jpg format. Figure(s) 2,3,5 are reduced in resolution. Accepted 14 Jan 2003 to the Astronomical Journa