II coupDefouet International Congress

Coordinació: Lluís Bosch i Mireia Freixa[eng] The I coupDefouet International Congress was held in June 2013 to celebrate the tenth anniversary of coupDefouet, the magazine of the Art Nouveau European Route, an association of municipalities and other entities to promote and disseminate Modernista or Art Nouveau heritage. Its success and excellent scientific results led to its continuity with a second congress, held in 2015, whose acts are presented in this volume of Singularities. The congresses are organised by the Urban Landscape Institute (IMPUiQV) of the Barcelona City Council in partnership with GRACMON, a consolidated research group of the University of Barcelona.[cat] El primer Congrés Internacional coupDefouet se celebrà el juny de l’any 2013 per commemorar els deu anys de l’aparició de coupDefouet, la revista de la Ruta Europea del Modernisme, una associació de municipis i altres entitats per a la promoció i la difusió del patrimoni modernista o Art Nouveau. Gràcies a l’èxit i als bons resultats científics es va apostar per la seva continuïtat amb un segon congrés que tingué lloc el 2015, les actes del qual presentem en aquest volum de Singularitats. Aquestes jornades estan organitzades per l’Institut Municipal del Paisatge Urbà i la Qualitat de Vida de l’Ajuntament de Barcelona, amb la col·laboració de GRACMON, grup de recerca consolidat de la Universitat de Barcelona

Standard specification for ventilation hygiene

SIGLEAvailable from British Library Document Supply Centre-DSC:f99/2014 / BLDSC - British Library Document Supply CentreGBUnited Kingdo

Review of GNSS-R instruments and tools developed at the Universitat Politecnica de Catalunya-Barcelona Tech

Reflectometry using Global Navigation Satellite System's signals of opportunity (GNSS-R) was originally conceived in the early 90s for mesoscale altimetry, and since then, many studies have shown its applicability to other remote sensing applications. In 2002, the Remote Sensing Lab at the Universitat Politècnica de Catalunya started working in Global Navigation Satellite Reflectometry (GNSS-R) to perform a more accurate correction of the sea state when retrieving sea surface salinity from L-band microwave radiometer data (e.g. SMOS). During these 12 years, the lab has developed ground-based, airborne, balloon, and spaceborne GNSS-R payloads for a number of different applications. This paper provides an overview of the families of instruments, and the design choices made.Peer Reviewe

Rare variants in calcium homeostasis modulator 1 (CALHM1) found in early onset Alzheimer's disease patients alter calcium homeostasis.

Calcium signaling in the brain is fundamental to the learning and memory process and there is evidence to suggest that its dysfunction is involved in the pathological pathways underlying Alzheimer's disease (AD). Recently, the calcium hypothesis of AD has received support with the identification of the non-selective Ca2+-permeable channel CALHM1. A genetic polymorphism (p. P86L) in CALHM1 reduces plasma membrane Ca2+ permeability and is associated with an earlier age-at-onset of AD. To investigate the role of CALHM1 variants in early-onset AD (EOAD), we sequenced all CALHM1 coding regions in three independent series comprising 284 EOAD patients and 326 controls. Two missense mutations in patients (p.G330D and p.R154H) and one (p.A213T) in a control individual were identified. Calcium imaging analyses revealed that while the mutation found in a control (p.A213T) behaved as wild-type CALHM1 (CALHM1-WT), a complete abolishment of the Ca2+ influx was associated with the mutations found in EOAD patients (p.G330D and p.R154H). Notably, the previously reported p. P86L mutation was associated with an intermediate Ca2+ influx between the CALHM1-WT and the p.G330D and p.R154H mutations. Since neither expression of wild-type nor mutant CALHM1 affected amyloid ß-peptide (Aß) production or Aß-mediated cellular toxicity, we conclude that rare genetic variants in CALHM1 lead to Ca2+ dysregulation and may contribute to the risk of EOAD through a mechanism independent from the classical Aß cascad

Effect of CALHM1 on <b>Aβ</b>_1-42- induced cell toxicity.

<p>HEK-293 cells over-expressing CALHM1-WT, CALHM1-G330D and CALHM1-P86L were treated with 2 μM Aβ_1-42 oligomers for 24h. Cell viability was measured by MTT reduction. Data are the mean ± SEM of three independent experiments performed in triplicate. * <i>P</i><0.05 vs untreated cells by student t test.</p

Effect of CALHM1 mutations on Ca²⁺ influx.

<p><b>A</b>. HEK-293 cells transfected with GFP, CALHM1-WT, CALHM1-P86L, CALHM1-R154H, CALHM1-A213T and CALHM1-G330D were loaded with Fura-2 and tested for Ca²⁺ influx into the cytoplasm following the removal of Ca²⁺ from the extracellular media. Time course of Ca²⁺ signals normalized to the value before the addition of Ca²⁺ and expressed as the mean±S.E.M. are shown. <b>B</b>. Mean peak Ca²⁺ signals for the different conditions are shown. Number of cells analysed are given for each condition. * <i>P</i><0.05 <i>vs</i> GFP; ** <i>P</i><0.01 <i>vs</i> GFP, one-way ANOVA and Bonferroni <i>post </i><i>hoc</i>.</p

cDf International Congress : proceedings : actes, actas : actes, Barcelona Jun. 2013

El Congrés Internacional coupDefouet Barcelona 2013 ha estat una iniciativa de l'Art Nouveau European Route – Ruta Europea del Modernisme, organitzada per l'Institut del Paisatge Urbà de l'Ajuntament de Barcelona i el grup de recerca GRACMON de la Universitat de BarcelonaCoordinació: Lluís Bosch, Mireia Freixa.[eng] In 2013 the magazine coupDefouet reached its first decade of existence. As a way of celebrating this, the Art Nouveau European Route – the association of cities and other local institutions for the promotion and diffusion of Art Nouveau heritage that created the magazine – organised a magnificent international congress as a framework for scientific exchange and raising public awareness. The first coupDefouet International Congress on Art Nouveau was held in Barcelona, the city from which coupDefouet is published and one of the undisputable world capitals of Art Nouveau. This volume of the Singularitats series comprises the proceedings of that event.[cat] L’any 2013 va fer deu anys de l’aparició de coupDefouet, la revista de la Ruta Europea del Modernisme, una associació de municipis i altres entitats locals per a la promoció i la difusió del patrimoni modernista o Art Nouveau. Per commemorar-ho, s’organitzà un congrés internacional amb l’objectiu de contribuir al coneixement científic i la difusió d’aquest moviment artístic. El primer Congrés Internacional coupDefouet se celebrà a Barcelona, la ciutat en què s’edita coupDefouet i una de les capitals indiscutibles de l’Art Nouveau. En aquest volum de Singularitats s’apleguen les actes d’aquell esdeveniment

Rare variants in calcium homeostasis modulator 1 (CALHM1) found in early onset alzheimer's disease patients alter calcium homeostasis

Calcium signaling in the brain is fundamental to the learning and memory process and there is evidence to suggest that its dysfunction is involved in the pathological pathways underlying Alzheimer’s disease (AD). Recently, the calcium hypothesis of AD has received support with the identification of the non-selective Ca2+-permeable channel CALHM1. A genetic polymorphism (p. P86L) in CALHM1 reduces plasma membrane Ca2+ permeability and is associated with an earlier age-at-onset of AD. To investigate the role of CALHM1 variants in early-onset AD (EOAD), we sequenced all CALHM1 coding regions in three independent series comprising 284 EOAD patients and 326 controls. Two missense mutations in patients (p.G330D and p.R154H) and one (p.A213T) in a control individual were identified. Calcium imaging analyses revealed that while the mutation found in a control (p.A213T) behaved as wild-type CALHM1 (CALHM1-WT), a complete abolishment of the Ca2+ influx was associated with the mutations found in EOAD patients (p.G330D and p.R154H). Notably, the previously reported p. P86L mutation was associated with an intermediate Ca2+ influx between the CALHM1-WT and the p.G330D and p.R154H mutations. Since neither expression of wild-type nor mutant CALHM1 affected amyloid ß-peptide (Aß) production or Aß-mediated cellular toxicity, we conclude that rare genetic variants in CALHM1 lead to Ca2+ dysregulation and may contribute to the risk of EOAD through a mechanism independent from the classical Aß cascade.This study was supported by grants from Instituto de Salud Carlos III (PI12/01311, PI10/000587, Red HERACLES RD12/0042/0014), Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED, Spain), Spanish Ministry of Economy and Competiveness (SAF2012-38140), FEDER Funds, and Generalitat de Catalunya (SGR05-266). Council of the Academy of Finland, EVO grant 5772708 of Kuopio University Hospital, the Strategic Funding of the University on Eastern Finland (UEF-Brain) (to M.H and H.S). M.A.V. is the recipient of an ICREA Academia Awar