Genomic landscape characterization of large granular lymphocyte leukemia with a systems genetics approach

Non peer reviewe

Identification of differentially expressed small RNAs and prediction of target genes in Italian Large White pigs with divergent backfat deposition

The identification of the molecular mechanisms regulating pathways associated with the potential for fat deposition in pigs can lead to the detection of key genes and markers for the genetic improvement of fat traits. Interactions of microRNAs (miRNAs) with target RNAs regulate gene expression and modulate pathway activation in cells and tissues. In pigs, miRNA discovery is far from saturation, and the knowledge of miRNA expression in backfat tissue and particularly of the impact of miRNA variations is still fragmentary. Using RNA-seq, we characterized the small RNA (sRNA) expression profiles in Italian Large White pig backfat tissue. Comparing two groups of pigs divergent for backfat deposition, we detected 31 significant differentially expressed (DE) sRNAs: 14 up-regulated (including ssc-miR-132, ssc-miR-146b, ssc-miR-221-5p, ssc-miR-365-5p and the moRNA ssc-moR-21-5p) and 17 down-regulated (including ssc-miR-136, ssc-miR-195, ssc-miR-199a-5p and ssc-miR-335). To understand the biological impact of the observed miRNA expression variations, we used the expression correlation of DE miRNA target transcripts expressed in the same samples to define a regulatory network of 193 interactions between DE miRNAs and 40 DE target transcripts showing opposite expression profiles and being involved in specific pathways. Several miRNAs and mRNAs in the network were found to be expressed from backfat-related pig QTL. These results are informative for the complex mechanisms influencing fat traits, shed light on a new aspect of the genetic regulation of fat deposition in pigs and facilitate the prospective implementation of innovative strategies of pig genetic improvement based on genomic markers

Classificação, densidade e parâmetros nutricionais do milho.

Projeto/Plano de Ação: 11.11.11.111

Correlação entre resultados de densidade e classificação de milho realizado por duas empresas.

Projeto/Plano de Ação: 03.10.01.03

Birth of an ocean in the Red Sea: Initial pangs

We obtained areal variations of crustal thickness, magnetic intensity, and degree of melting of the sub- axial upwelling mantle at Thetis and Nereus Deeps, the two northernmost axial segments of initial oceanic crustal accretion in the Red Sea, where Arabia is separating from Africa. The initial emplacement of oceanic crust occurred at South Thetis and Central Nereus roughly $2.2 and$2 Ma, respectively, and is taking place today in the northern Thetis and southern Nereus tips. Basaltic glasses major and trace element com- position suggests a rift-to-drift transition marked by magmatic activity with typical MORB signature, with no contamination by continental lithosphere, but with slight differences in mantle source composition and/or potential temperature between Thetis and Nereus. Eruption rate, spreading rate, magnetic intensity, crustal thickness and degree of mantle melting were highest at both Thetis and Nereus in the very initial phases of oceanic crust accretion, immediately after continental breakup, probably due to fast mantle upwelling enhanced by an initially strong horizontal thermal gradient. This is consistent with a rift model where the lower continental lithosphere has been replaced by upwelling asthenosphere before continental rupturing, implying depth-dependent extension due to decoupling between the upper and lower lithosphere with man- tle-lithosphere-necking breakup before crustal-necking breakup. Independent along-axis centers of upwell- ing form at the rifting stage just before oceanic crust accretion, with buoyancy-driven convection within a hot, low viscosity asthenosphere. Each initial axial cell taps a different asthenospheric source and serves as nucleus for axial propagation of oceanic accretion, resulting in linear segments of spreading

Real Time Marine Data Acquisition: The Coastal Oceanographic Observatory Network in the Adriatic sea.

not availabl

Therapeutic strategies in severe neuropsychiatric systemic lupus erythematosus: experience from a tertiary referral centre.

The management of neuropsychiatric systemic lupus erythematosus (NPSLE) still remains empirical and based on clinical experience due to the lack of randomized controlled trials. Objective: to report the experience accumulated in a single tertiary referral centre about treatment of severe cases of NPSLE patients and to discuss therapeutic strategies on the background of EULAR recommendations. Methods: retrospective analysis of all consecutive cases of severe NPSLE treated in our centre since 1990 to 2010, satisfying the 1999 ACR criteria. Results: among 633 SLE patients who consecutively attended our centre, 231 (36%) displayed at least one neuropsychiatric (NP) manifestation for a total of 408 events attributable to SLE. Thirty-one patients (4.8%), 27 females and 4 males, experienced 35 major NP events requiring immunosuppressive therapy (including 3 relapses and 1 new event). An aggressive immunosuppressive strategy was applied to those patients with an immune mediated inflammatory NP event and to those patients with an increased disease activity as judged by ECLAM and SLEDAI scores. Overall at the end of the therapy 74% of the patients reached clinical remission or significant improvement of their symptoms measured by mean SLEDAI (from 10.09±1.09 to 2.04±0.52, P<0.0001) and ECLAM (from 4±0.34 to 1.38±0.37, P<0.001) scores. Conclusions: the prevalence of NP involvement, described in our case series, is similar to those reported in literature as well as the treatment strategies applied. Nowadays, it is not possible to establish a standardized approach for each single NPSLE manifestation, and different therapeutic strategies must be tailored taking into account the most probable pathogenic mechanism involved, the general disease activity background, the co-morbidities, the type and the stage of the systemic involvement

Studio multiparametrico dell’arcipelago di Panarea (Isole Eolie): primi risultati dei rilievi geodetici, geofisici e geologici

La recente crisi esalativa di Panarea ha indotto ad effettuare uno studio sistematico dell’area con tecniche GPS, batimetrie ad alta risoluzione, rilievi gravimetrici e di suscettività magnetica, rilievi geologici sottomarini e terrestri. I rilievi batimetrici hanno permesso di individuare la posizione e la estensione dei centri esalativi principali e il campo di fratturazione; quelli gravimetrici e di suscettività magnetica mostrano anomalie nel fianco occidentale di Panarea (formazione Punta Cardosi). I dati GPS, acquisiti sia sulla rete locale che nelle stazioni continue di Lisca Bianca e Panarea, mostrano una deformazione dell’area che è in accordo con il trend del campo di fratturazione attivo stimato dai rilievi geologici sottomarini e terrestri

Evaluation of Precision Livestock Technology and Human Scoring of Nursery Pigs in a Controlled Immune Challenge Experiment

The objectives were to determine the sensitivity, specificity, and cutoff values of a visual-based precision livestock technology (NUtrack), and determine the sensitivity and specificity of sickness score data collected with the live observation by trained human observers. At weaning, pigs (n = 192; gilts and barrows) were randomly assigned to one of twelve pens (16/pen) and treatments were randomly assigned to pens. Sham-pen pigs all received subcutaneous saline (3 mL). For LPS-pen pigs, all pigs received subcutaneous lipopolysaccharide (LPS; 300 µg/kg BW; E. coli O111:B4; in 3 mL of saline). For the last treatment, eight pigs were randomly assigned to receive LPS, and the other eight were sham (same methods as above; half-and-half pens). Human data from the day of the challenge presented high true positive and low false positive rates (88.5% sensitivity; 85.4% specificity; 0.871 Area Under Curve, AUC), however, these values declined when half-and-half pigs were scored (75% sensitivity; 65.5% specificity; 0.703 AUC). Precision technology measures had excellent AUC, sensitivity, and specificity for the first 72 h after treatment and AUC values were \u3e0.970, regardless of pen treatment. These results indicate that precision technology has a greater potential for identifying pigs during a natural infectious disease event than trained professionals using timepoint sampling

Injection of a Body into a Geodesic: Lessons Learnt from the LISA Pathfinder Case

Launch lock and release mechanisms constitute a common space business, however, some science missions due to very challenging functional and performance requirements need the development and testing of dedicated systems. In the LISA Pathfinder mission, a gold-coated 2-kg test mass must be injected into a nearly pure geodesic trajectory with a minimal residual velocity with respect to the spacecraft. This task is performed by the Grabbing Positioning and Release Mechanism, which has been tested on-ground to provide the required qualification. In this paper, we describe the test method that analyzes the main contributions to the mechanism performance and focuses on the critical parameters affecting the residual test mass velocity at the injection into the geodesic trajectory. The test results are also presented and discussed