Quantitative assay for farnesol and the aromatic fusel alcohols from the fungus \u3ci\u3eCandida albicans\u3c/i\u3e

The dimorphic fungus Candida albicans is a commensal and opportunistic fungal pathogen of humans. It secretes at least four small lipophilic molecules, farnesol and three aromatic fusel alcohols. Farnesol has been identified as both a quorum sensing molecule (QSM) and a virulence factor. Our gas chromatography (GC)-based assay for these molecules exhibits high throughput, prevention of analyte loss by avoiding filtration and rotary evaporation, simultaneous cell lysis and analyte extraction by ethyl acetate, and the ability to compare whole cultures with their cell pellets and supernatants. Farnesol synthesis and secretion were separable phenomena and pellet:supernatant ratios for farnesol were high, up to 12:1. The assay was validated in terms of precision, specificity, ruggedness, accuracy, solution stability, detection limits (DL), quantitation limits (QL), and dynamic range. The DL for farnesol was 0.02 ng/μl (0.09 μM). Measurement quality was assessed by the relative error of the whole culture versus the sum of pellet and supernatant fractions (WPS). C. albicans strain SC5314 grown at 30 °C in complex and defined media (YPD and mRPMI) was assayed in biological triplicate 17 times over 3 days. Farnesol and the three aromatic fusel alcohols can be measured in the same assay. The levels of all four are greatly altered by the growth medium chosen. Significantly, the three fusel alcohols are synthesized during stationary phase, not during growth. They are secreted quickly without being retained in the cell pellet and may accumulate up to mM concentrations

Biomass and nutrient dynamics associated with slash fires in neotropical dry forests

Unprecedented rates of deforestation and biomass burning in tropical dry forests are dramatically influencing biogeochemical cycles, resulting in resource depletion, declines in biodiversity, and atmospheric pollution. We quantified the effects of defores- tation and varying levels of slash-fire severity on nutrient losses and redistribution in a second-growth tropical dry forest ("Caatinga") near Serra Talhada, Pernambuco, Brazil. Total aboveground biomass prior to burning was 74 Mg/ha. Nitrogen and phosphorus concentrations were highest in litter, leaves attached to slash, and fine wood debris (<0.64 cm diameter). While these components comprised only 30% of the prefire aboveground biomass, they accounted for -60% of the aboveground pools of N and P. Three experi- mental fires were conducted during the 1989 burning season. In these treatments con- sumption was 78, 88, and 95% of the total aboveground biomass. As much as 96% of the prefire aboveground N and C pools and 56% of the prefire aboveground P pool was lost during combustion processes. Nitrogen losses exceeded 500 kg/ha and P losses exceeded 20 kg/ha in the fires of the greatest severity. With increasing fire severity, the concentrations of N and P in ash decreased while the concentration of Ca increased. This indicates greater amounts of these nutrients were volatilized (i.e., greater ecosystem losses occurred) with increasing fire severity. Following fire, up to 47% of the residual aboveground N and 84% of the residual aboveground P were in the form of ash, which was quickly lost from the site via wind erosion. Fires appeared to have a minor immediate effect on total N, C, or P in the soils. However, soils in forests with no history of cultivation had significantly higher concentrations of C and P than second-growth forests. Based upon the measured losses of nutrients from these single slash-burning events, it would likely require a century or more of fallow for reaccumulation to occur. However, current fallow periods in this region are 15 yr or less

Why, what, and how? case study on law, risk, and decision making as necessary themes in built environment teaching

The paper considers (and defends) the necessity of including legal studies as a core part of built environment undergraduate and postgraduate curricula. The writer reflects upon his own experience as a lawyer working alongside and advising built environment professionals in complex land remediation and site safety management situations in the United Kingdom and explains how themes of liability, risk, and decision making can be integrated into a practical simulation in order to underpin more traditional lecture-based law teaching. Through reflection upon the writer's experiments with simulation-based teaching, the paper suggests some innovations that may better orientate law teaching to engage these themes and, thereby, enhance the relevance of law studies to the future needs of built environment professionals in practice.</p

The High Arctic in Extreme Winters: Vortex, Temperature, and MLS and ACE-FTS Trace Gas Evolution

The first three Canadian Arctic Atmospheric Chemistry Experiment (ACE) Validation Campaigns at Eureka (80° N, 86° W) were during two extremes of Arctic winter variability: Stratospheric sudden warmings (SSWs) in 2004 and 2006 were among the strongest, most prolonged on record; 2005 was a record cold winter. New satellite measurements from ACE-Fourier Transform Spectrometer (ACE-FTS), Sounding of the Atmosphere using Broadband Emission Radiometry, and Aura Microwave Limb Sounder (MLS), with meteorological analyses and Eureka lidar and radiosonde temperatures, are used to detail the meteorology in these winters, to demonstrate its influence on transport and chemistry, and to provide a context for interpretation of campaign observations. During the 2004 and 2006 SSWs, the vortex broke down throughout the stratosphere, reformed quickly in the upper stratosphere, and remained weak in the middle and lower stratosphere. The stratopause reformed at very high altitude, above where it could be accurately represented in the meteorological analyses. The 2004 and 2006 Eureka campaigns were during the recovery from the SSWs, with the redeveloping vortex over Eureka. 2005 was the coldest winter on record in the lower stratosphere, but with an early final warming in mid-March. The vortex was over Eureka at the start of the 2005 campaign, but moved away as it broke up. Disparate temperature profile structure and vortex evolution resulted in much lower (higher) temperatures in the upper (lower) stratosphere in 2004 and 2006 than in 2005. Satellite temperatures agree well with Eureka radiosondes, and with lidar data up to 50–60 km. Consistent with a strong, cold upper stratospheric vortex and enhanced radiative cooling after the SSWs, MLS and ACE-FTS trace gas measurements show strongly enhanced descent in the upper stratospheric vortex during the 2004 and 2006 Eureka campaigns compared to that in 2005

Fusing face and body display for Bi-modal emotion recognition: Single frame analysis and multi-frame post integration

This paper presents an approach to automatic visual emotion recognition from two modalities: expressive face and body gesture. Pace and body movements are captured simultaneously using two separate cameras. For each face and body image sequence single "expressive" frames are selected manually for analysis and recognition of emotions. Firstly, individual classifiers are trained from individual modalities for mono-modal emotion recognition. Secondly, we fuse facial expression and affective body gesture information at the feature and at the decision-level. In the experiments performed, the emotion classification using the two modalities achieved a better recognition accuracy outperforming the classification using the individual facial modality. We further extend the affect analysis into a whole image sequence by a multi-frame post integration approach over the single frame recognition results. In our experiments, the post integration based on the fusion of face and body has shown to be more accurate than the post integration based on the facial modality only. © Springer-Verlag Berlin Heidelberg 2005

Planck's Dusty GEMS: Gravitationally lensed high-redshift galaxies discovered with the Planck survey

We present an analysis of 11 bright far-IR/submm sources discovered through a combination of the Planck survey and follow-up Herschel-SPIRE imaging. Each source has a redshift z=2.2-3.6 obtained through a blind redshift search with EMIR at the IRAM 30-m telescope. Interferometry obtained at IRAM and the SMA, and optical/near-infrared imaging obtained at the CFHT and the VLT reveal morphologies consistent with strongly gravitationally lensed sources. Additional photometry was obtained with JCMT/SCUBA-2 and IRAM/GISMO at 850 um and 2 mm, respectively. All objects are bright, isolated point sources in the 18 arcsec beam of SPIRE at 250 um, with spectral energy distributions peaking either near the 350 um or the 500 um bands of SPIRE, and with apparent far-infrared luminosities of up to 3x10^14 L_sun. Their morphologies and sizes, CO line widths and luminosities, dust temperatures, and far-infrared luminosities provide additional empirical evidence that these are strongly gravitationally lensed high-redshift galaxies. We discuss their dust masses and temperatures, and use additional WISE 22-um photometry and template fitting to rule out a significant contribution of AGN heating to the total infrared luminosity. Six sources are detected in FIRST at 1.4 GHz. Four have flux densities brighter than expected from the local far-infrared-radio correlation, but in the range previously found for high-z submm galaxies, one has a deficit of FIR emission, and 6 are consistent with the local correlation. The global dust-to-gas ratios and star-formation efficiencies of our sources are predominantly in the range expected from massive, metal-rich, intense, high-redshift starbursts. An extensive multi-wavelength follow-up programme is being carried out to further characterize these sources and the intense star-formation within them.Comment: A&A accepte

Sampling Local Fungal Diversity in an Undergraduate Laboratory using DNA Barcoding

Traditional methods for fungal species identification require diagnostic morphological characters and are often limited by the availability of fresh fruiting bodies and local identification resources. DNA barcoding offers an additional method of species identification and is rapidly developing as a critical tool in fungal taxonomy. As an exercise in an undergraduate biology course, we identified 9 specimens collected from the Hendrix College campus in Conway, Arkansas, USA to the genus or species level using morphology. We report that DNA barcoding targeting the internal transcribed spacer (ITS) region supported several of our taxonomic determinations and we were able to contribute 5 ITS sequences to GenBank that were supported by vouchered collection information. We suggest that small-scale barcoding projects are possible and that they have value for documenting fungal diversity