Doctor of Philosophy

dissertationThe relationship between law and philosophy is easily and often overstated. In this dissertation, I shed some light on the appropriate relationship between law and philosophy. The primary problem I identify is confusing a conceptual issue for a practical one. While theory can help clarify almost any problem, it has limits. And it is just as important to understand the limits as to understand the theory. This dissertation begins to identify some of those limits as they relate to legal issues. The dissertation explores the appropriate use of philosophy of language in arguments concerning how to interpret legal text;s, especially constitutions. It then points out the futility of attempting to clarify legal doctrine with more precise definitions, such as definitions of "religion" and "commercial speech." Finally, it explores the relationship between philosophical debates concerning free will and legal debates over the extent to which free will is relevant to legal accountability. The legal lesson is that current trends to treat brain science as dispositive of issues concerning legal accountability should be viewed with skepticism. In the end, I hope to convince the reader that, while the methods of philosophy are valuable to those analyzing law, the conclusions of philosophers are more likely to cause confusion than clarity if they are imported into the decisions judges make in interpreting law. For those engaged in interdisciplinary work in the two areas, the message is not "stop," but "proceed with caution.

Development of a bedrest muscle stress apparatus

In attempting further to define the deleterious effects of spaceflight on the human body, measurement systems and techniques were devised to determine the loss of skeletal muscle strength and tone as a result of spaceflight exposure. In order to determine how the muscle degradation process progresses with time during nonuse, a system for measuring muscle stress during bedrest was developed. The Bedrest Muscle Stress Apparatus is configured to slip snugly over the foot board of a standard hospital bed. Data collected with this device correlated well with pre- and post-bedrest data collected with the original skeletal muscle stress apparatus

Cloning and expression of a human kinesin heavy chain gene: interaction of the COOH-terminal domain with cytoplasmic microtubules in transfected CV-1 cells.

To understand the interactions between the microtubule-based motor protein kinesin and intracellular components, we have expressed the kinesin heavy chain and its different domains in CV-1 monkey kidney epithelial cells and examined their distributions by immunofluorescence microscopy. For this study, we cloned and sequenced cDNAs encoding a kinesin heavy chain from a human placental library. The human kinesin heavy chain exhibits a high level of sequence identity to the previously cloned invertebrate kinesin heavy chains; homologies between the COOH-terminal domain of human and invertebrate kinesins and the nonmotor domain of the Aspergillus kinesin-like protein bimC were also found. The gene encoding the human kinesin heavy chain also contains a small upstream open reading frame in a G-C rich 5' untranslated region, features that are associated with translational regulation in certain mRNAs. After transient expression in CV-1 cells, the kinesin heavy chain showed both a diffuse distribution and a filamentous staining pattern that coaligned with microtubules but not vimentin intermediate filaments. Altering the number and distribution of microtubules with taxol or nocodazole produced corresponding changes in the localization of the expressed kinesin heavy chain. The expressed NH2-terminal motor and the COOH-terminal tail domains, but not the alpha-helical coiled coil rod domain, also colocalized with microtubules. The finding that both the kinesin motor and tail domains can interact with cytoplasmic microtubules raises the possibility that kinesin could crossbridge and induce sliding between microtubules under certain circumstances

Big five personality characteristics and commitment levels in romantic relationships

While numerous empirical studies have looked at commitment levels across various aspects of life, little research has attempted to integrate these disparate literatures. Accordingly, this study attempts to address this gap by adapting a taxonomy of commitment commonly used in the workplace to the analysis of commitment in romantic relationships. Additionally, the relationship between personality and levels of commitment in romantic relationships were investigated utilizing the Big Five personality factors. Results indicated that personality does relate to commitment and that the affective (emotional) and continuance (economic) facets of commitment have specific relevance to understanding commitment expressed in romantic relationships. Gender similarities and differences, as well as directions for future research, are discussed

Microtubule Interaction Site of the Kinesin Motor

AbstractKinesin and myosin are motor proteins that share a common structural core and bind to microtubules and actin filaments, respectively. While the actomyosin interface has been well studied, the location of the microtubule-binding site on kinesin has not been identified. Using alanine-scanning mutagenesis, we have found that microtubule-interacting kinesin residues are located in three loops that cluster in a patch on the motor surface. The critical residues are primarily positively charged, which is consistent with a primarily electrostatic interaction with the negatively charged tubulin molecule. The core of the microtubule-binding interface resides in a highly conserved loop and helix (L12/α5) that corresponds topologically to the major actin-binding domain of myosin. Thus, kinesin and myosin have developed distinct polymer-binding domains in a similar region with respect to their common catalytic cores

December 1965

Massachusetts Turf and Lawn Grass Council Better Turf Through Research and Educatio

From START to FINISH : the influence of osmotic stress on the cell cycle

Peer reviewedPublisher PD

Translating global recommendations on HIV and infant feeding to the local context: the development of culturally sensitive counselling tools in the Kilimanjaro Region, Tanzania

BACKGROUND: This paper describes the process used to develop an integrated set of culturally sensitive, evidence-based counselling tools (job aids) by using qualitative participatory research. The aim of the intervention was to contribute to improving infant feeding counselling services for HIV positive women in the Kilimanjaro Region of Tanzania. METHODS: Formative research using a combination of qualitative methods preceded the development of the intervention and mapped existing practices, perceptions and attitudes towards HIV and infant feeding (HIV/IF) among mothers, counsellors and community members. Intervention Mapping (IM) protocol guided the development of the overall intervention strategy. Theories of behaviour change, a review of the international HIV/IF guidelines and formative research findings contributed to the definition of performance and learning objectives. Key communication messages and colourful graphic illustrations related to infant feeding in the context of HIV were then developed and/or adapted from existing generic materials. Draft materials were field tested with intended audiences and subjected to stakeholder technical review. RESULTS: An integrated set of infant feeding counselling tools, referred to as 'job aids', was developed and included brochures on feeding methods that were found to be socially and culturally acceptable, a Question and Answer Guide for counsellors, a counselling card on the risk of transmission of HIV, and an infant feeding toolbox for demonstration. Each brochure describes the steps to ensure safer infant feeding using simple language and images based on local ideas and resources. The brochures are meant to serve as both a reference material during infant feeding counselling in the ongoing prevention of mother to child transmission (pMTCT) of HIV programme and as take home material for the mother. CONCLUSION: The study underscores the importance of formative research and a systematic theory based approach to developing an intervention aimed at improving counselling and changing customary feeding practices. The identification of perceived barriers and facilitators for change contributed to developing the key counselling messages and graphics, reflecting the socio-economic reality, cultural beliefs and norms of mothers and their significant others