Opis nove vrste, Phoxinellus dalmaticus (Cyprinidae: Leuciscinae), iz rijeke Čikole u slivu rijeke Krke, Jadranski bazen (Hrvatska)

Phoxinellus dalmaticus, new species, is described from the Čikola River in the Krka River system in Croatia. It is distinguished from congeners by a suite of characters which includes a naked body with scales only in a relatively short, often interrupted, lateral line series (sq. l. 18–44) formed from both pored (l. l. 16–29) and unpored scales, a low number of vertebrae (37 or 38 total) with 21 vertebrae in the abdominal and 16 or 17 in the caudal region, a shallowly forked caudal fin with considerably rounded lobes and the lack of a foramen between the cleithrum and coracoid.Nova vrsta Phoxinellus dalmaticus opisana je iz rijeke Čikole u slivu rijeke Krke u Hrvatskoj. Razlikuje se od srodnih vrsta nizom osobina koje uključuju golo tijelo s ljuskama samo u relativno kratkoj, često isprekidanoj lateralnoj seriji linija (sq. l. 18–44), koje čine ljuske s porama (l. l. 16–29) i bez njih, mali broj kralježaka (ukupno 37 ili 38) s 21 kralješkom u abdominalnom i 16 ili 17 u kaudalnom dijelu, plitko urezana repna peraja sa znatno zaobljenim režnjevima i nepostojanje otvora između kleitruma i korakoidne kosti

A recent record of Romanogobio antipai (Actinopterygii, Cyprinidae, Gobioninae) from the Danube River in Bulgaria

The Danube delta gudgeon, Romanogobio antipai, has been considered to be extinct because there were no reliable recent observations. The latest record confirmed by a voucher specimen dating from 1992. We report here on a specimen of R. antipai collected in 2016 in the Bulgarian sector of the Danube main stream using a bottom drift net at a depth of 8 m. The species determination is supported by morphological examination including discriminant and cluster analyses in comparison with three syntypes and five non-type specimens of R. antipai, samples of the R. kesslerii species complex and R. vladykovi. Romanogobio antipai most clearly differs from both R. kesslerii and R. vladykovi by proportional measurements (caudal peduncle depth, head width, eye horizontal diameter, and interorbital width), from R. kesslerii also by the number of scales above and below the lateral line (6 and 4, respectively, (vs. commonly 5 and 3), and from R. vladykovi, also by 8½ branched dorsal-fin rays (vs. 7½) and the vertebral caudal region longer than the abdominal vertebral region (abdominal+caudal vertebrae 19+21 or 20+21, vs. commonly 20+20 or variants with a caudal region shorter than the abdominal one). The possibility that R. antipai represents a deep-water cophenotype of either R. kesslerii or R. vladykovi, cannot be excluded. The new record demonstrates that R. antipai is still extant in the lower Danube but may be restricted to greater depths in the main channel and the deltaic branches

Freshwater ecoregions of the world: A new map of biogeographic units for freshwater biodiversity conservation

We present a new map depicting the first global biogeographic regionalization of Earth's freshwater systems. This map of freshwater ecoregions is based on the distributions and compositions of freshwater fish species and incorporates major ecological and evolutionary patterns. Covering virtually all freshwater habitats on Earth, this ecoregion map, together with associated species data, is a useful tool for underpinning global and regional conservation planning efforts (particularly to identify outstanding and imperiled freshwater systems); for serving as a logical framework for large-scale conservation strategies; and for providing a global-scale knowledge base for increasing freshwater biogeographic literacy. Preliminary data for fish species compiled by ecoregion reveal some previously unrecognized areas of high biodiversity, highlighting the benefit of looking at the world's freshwaters through a new framework.La lista completa de autores que integran el documento puede consultarse en el archivo.Facultad de Ciencias Naturales y Muse

Freshwater ecoregions of the world: A new map of biogeographic units for freshwater biodiversity conservation

We present a new map depicting the first global biogeographic regionalization of Earth's freshwater systems. This map of freshwater ecoregions is based on the distributions and compositions of freshwater fish species and incorporates major ecological and evolutionary patterns. Covering virtually all freshwater habitats on Earth, this ecoregion map, together with associated species data, is a useful tool for underpinning global and regional conservation planning efforts (particularly to identify outstanding and imperiled freshwater systems); for serving as a logical framework for large-scale conservation strategies; and for providing a global-scale knowledge base for increasing freshwater biogeographic literacy. Preliminary data for fish species compiled by ecoregion reveal some previously unrecognized areas of high biodiversity, highlighting the benefit of looking at the world's freshwaters through a new framework.La lista completa de autores que integran el documento puede consultarse en el archivo.Facultad de Ciencias Naturales y Muse

Freshwater ecoregions of the world: A new map of biogeographic units for freshwater biodiversity conservation

We present a new map depicting the first global biogeographic regionalization of Earth's freshwater systems. This map of freshwater ecoregions is based on the distributions and compositions of freshwater fish species and incorporates major ecological and evolutionary patterns. Covering virtually all freshwater habitats on Earth, this ecoregion map, together with associated species data, is a useful tool for underpinning global and regional conservation planning efforts (particularly to identify outstanding and imperiled freshwater systems); for serving as a logical framework for large-scale conservation strategies; and for providing a global-scale knowledge base for increasing freshwater biogeographic literacy. Preliminary data for fish species compiled by ecoregion reveal some previously unrecognized areas of high biodiversity, highlighting the benefit of looking at the world's freshwaters through a new framework.La lista completa de autores que integran el documento puede consultarse en el archivo.Facultad de Ciencias Naturales y Muse

Alburnoides holciki Coad & Bogutskaya, 2012, new species

<i>Alburnoides holciki</i>, new species <p>(Figs. 1, 2)</p> <p> <b>Holotype</b> (Fig. 1). SNM 6788, female, 101.8 mm TL, 80.0 mm SL, AFGHANISTAN, Hari River at Herat (Herat is at 34°20’N, 62°12’E), 9 August 1974, coll. L. Jedlička.</p> <p> <b>Paratypes</b>. SNM 6788, 18 specimens, 49.6–92.6 mm SL, 8 males 49.6–70.2 mm SL and 10 females 56.4–92.6 mm SL, same data as holotype.</p> <p> <b>Non-type material</b> (Fig. 2). BMNH 1914.1.1:30-31, 2, 82.4–92.7 mm SL, IRAN, Kashaf River, Mashhad (Mashhad is at 36°18’N, 59°36’E), coll. P. Sykes (material dried at some point so not measurable in comparison with type series).</p> <p> <b>Diagnosis.</b> The species is distinguished by a combination of characters which includes a well-defined, sharp, scaleless or only slightly scaled, ventral keel; a short, slightly pointed snout; a terminal mouth with the tip of the mouth cleft on a level with the upper half of the pupil; a large eye (orbit width about equal to interorbital width), (46)47–51(55) lateral-line scales to posterior margin of hypurals (47–57 total lateral-line scales); 2.5–4.2 pharyngeal teeth; usually 8½ branched dorsal-fin rays; usually 13–16½ branched anal-fin rays; 40–42, usually 41, total vertebrae; caudal vertebral region longer than abdominal region (most frequent vertebral formulae 20+21, 20+22 and 19+21); and usually 13 or 14 predorsal vertebrae.</p> <p> <b>Description.</b> Holotype. A ventral keel between the pelvics and the anal-fin is well-developed, sharp and scaleless. There is a pelvic axillary scale and scales extend over the proximal bases of the anal-fin. The lateral line is decurved and only the last few scales are elevated and on the mid-caudal peduncle. Lateral-line scales to posterior margin of hypurals number 47 (49 total), scales above lateral line to dorsal-fin origin are 11, scales below lateral line to anal-fin origin are 4, scales below lateral line to pelvic-fin origin are 5, predorsal scales are 23, and circumpeduncle scales are 17.</p> <p>Dorsal-fin rays are 3 unbranched and 8½ branched, anal-fin rays are 3 unbranched and 13½ branched, branched pectoral-fin rays are 13, pelvic-fin branched rays are 7. The anal-fin origin is under the 7th branched ray of the dorsal-fin. There are 6 gill rakers on the entire first gill arch and the longest raker reaches the raker below when appressed. Pharyngeal teeth are –4.2 on the right 5th ceratobranchial. Total vertebrae are 41 (including 4 Weberian vertebrae and last complex centrum), abdominal vertebrae (including intermediate ones; precaudal vertebrae auctorum) are 20, caudal vertebrae are 21, and predorsal abdominal vertebrae (anterior to the first dorsal-fin pterygiophore) are 14.</p> <p> General topography of cephalic sensory canals and numbers of pores are typical of most <i>Alburnoides</i>, as described by Bogutskaya (1988). The supraorbital canal is not lengthened in its posterior section and has 10 (left) / 9 (right) pores, the infraorbital canal has 16/13 pores; the preopercular-mandibular canal is complete, with 15/15 pores, and the supratemporal canal is also complete, with 5 pores.</p> <p>The upper body profile is rounded while the lower profile is slightly more convex. The snout is short, slightly pointed, not stout. The mouth is upturned and terminal, the mouth cleft is straight, and its tip is on a level with the upper half of the pupil. Neither lower jaw nor upper jaw protrudes relative to each other. The caudal-fin lobes are pointed, the fin is clearly forked. Morphometrics are summarised in Table 1.</p> <p> The holotype in preservative is decoloured and discernible pigment is a light brown. Pigmentation consists of a darker back fading to a cream belly, pre- and post-dorsal-fin stripes on the back, small spots on the upper operculum and adjacently behind the eye that look like the remains of a dark lateral stripe, three rows of spots above the lateral line anteriorly before the pelvic-fin level, a dark line delimiting the hypaxial and epaxial muscle masses overlain by a diffuse stripe from the head to the tail, expanded on the tail base, a lateral line demarcated by pigment above and below it (the typical "stitched" pattern in many <i>Alburnoides</i> species), fins are mostly hyaline, faint pigment along the anterior dorsal-fin rays, a line of pigment along the proximal edge of the unbranched pectoral-fin ray and weak pigmentation on the first branched ray, and faint pigmentation on the caudal-fin centre branching distally to follow the inner margins of the fin fork.</p> <p>Paratypes. The ventral keel between the pelvics and anal-fin is a well-developed, sharp and protruding scaleless keel in 15 fish, with one scale behind the pelvic-fins (scaled along 1/4 of its length) in two fish, and with three scales (scaled along 1/3 of its length) in one fish.</p> <p>A pelvic axillary scale is present and the anal-fin base is proximally overlain by flank scales. Scales below the dorsal-fin on the upper flank are a vertical oval with rounded posterior, dorsal and ventral margins, and a wavy anterior margin. Scale radii are restricted to the posterior field encroaching laterally, circuli are fine and eccentric and the focus is anteriorly located. The lateral line is complete; it is decurved and only the last few scales are elevated and on the mid-caudal peduncle.</p> <p>Meristic characters showed no significant differences (p>0.5) between males and females and the data were combined with the exception of the predorsal scale count. Lateral-line scales to posterior margin of hypurals 46(1), 47(4), 48(6), 49(2), 50(1), 51(3) or 55(1) (48.8, 2.16); total lateral-line scales 47–53 (57 in one paratype); scales above lateral line to dorsal-fin origin 9(2), 10(14) or 11(2) (10.0, 0.49), scales below lateral line to pelvic-fin origin 4(3), 5(14) or 6(1) (4.9, 0.47), scales below lateral line to anal-fin origin 4(7) or 5(11) (4.6, 0.50), predorsal scales 20(1), 21(5) and 22(2) for males (21.1,0.64) and 20(1), 21(1), 22(2), 23(3), 24(1), 25(1) or 26(1) for females (22.9, 1.79 (p = 0.02), and circumpeduncular scales 16(2) or 17(16) (16.9, 0.32).</p> <p>Dorsal-fin unbranched rays 3 (in 12 paratypes) or 4 (in 6 paratypes), dorsal-fin branched rays 8½(15) or 9½(3) (mean 8.2, standard deviation 0.38), anal-fin unbranched rays 3, anal-fin branched rays 10½(1), 14½(6), 15½(8) or 16½(3) (14.6, 1.34) (14.8 and 0.73 if the abnormal number, 10½, is excluded), branched pectoral-fin rays 13(8), 14(9) or 15(1) (13.6, 0.61), and pelvic-fin branched rays 7(18). The dorsal-fin outer margin is truncate to slightly rounded and the anal-fin outer margin is slightly concave. The anal-fin origin is under the 5–7th branched ray of the dorsal-fin.</p> <p>Pharyngeal tooth counts are 2.5–4.2 in 6 fish, and in other 13 fish only the right 5th ceratobranchial was examined where –4.2 were found in 12 specimens and – 5.2 in one. Teeth are hooked at the tip and not serrated below it. The gut shape is a simple “S” with larger fish having a slight loop to the left anteriorly. Total gill rakers in the outer row on first left arch number 5(1), 6(4), 7(5), 8(7) or 9(1) (7.2, 1.04); gill rakers are not long and are widely spaced, touching the adjacent raker when appressed, often small and weakly developed.</p> <p>The total vertebrae are 40 (2), 41 (13) or 42 (3). Abdominal vertebrae are 19 (3), 20 (14) or 21 (1); predorsal abdominal vertebrae are 12 (1), 13 (13) or 14 (4); intermediate vertebrae are 2 (3), 3 (14) or 4 (1). Caudal vertebrae are 20 (1), 21 (13) or 22 (4). The vertebral formulae are 20+21 (11), 20+22 (3), 19+21 (3), 19+22 (1) or 21+20 (1). Thus, the caudal vertebral region most commonly (in all specimens but one) is longer then the abdominal region, the difference between abdominal and caudal counts being –1 (11), –2 (6), –3 (1) or 1 (1). A postcleithrum in the pectoral-fin skeleton is small and maybe absent on one or both sides.</p> <p>Canal pores counts were examined in 13 paratypes on both sides. The supraorbital canal has 8 (11), 9 (13) or 10 (2) pores, the infraorbital canal has 12 (1), 13 (2), 14 (9), 15 (9), 16 (1), 17 (2), 18 (1) or 20 (1) pores, the preopercular-mandibular canal has 14 (11), 15 (12), 16 (1) or 17 (2) pores, and the supratemporal canal (unpaired) has 5 (3), 6 (5), or 7 (5) pores.</p> <p>The body is markedly compressed. The upper body profile is moderately rounded while the lower profile is more convex or both profiles are about equally convex. The eye is large, orbit width about equal to interorbital width. The snout is short, slightly pointed, not stout. The mouth is terminal, with the tip of the mouth cleft on a level with the upper half of the pupil. Neither lower jaw nor upper jaw protrudes relative to each other, so no ‘chin’ is formed by the symphysis of the lower jaws. The mouth cleft is always turned upward, never horizontal, and the junction of the lower jaw and the quadrate is on about a vertical through the anterior eye margin. In those paratypes with an unbroken caudal-fin its lobes are pointed, the fin is clearly forked.</p> <p>Morphometrics are summarised in Table 1. The majority of morphometric characters are not significantly different between males and females as shown by values in Table 1. Exceptions are pectoral-fin and dorsal-fin lengths are longer in males (pectoral-fin often reaching the pelvic-fin origin), and preanal and pelvic-fin to anal-fin distances are longer in females.</p> <p>Colouration. Pigmentation in preserved fish is as described for the holotype but there are 3–4 rows of spots anteriorly on the flank and these rows may extend to the end of the pelvic-fin level or just beyond it. Additionally, there may be 1–2 rows of spots below the anterior lateral line in front of the pelvic-fin level and above the pectoralfin. The peritoneum is white-grey to light brown overall, the latter from distinct but crowded pale brown spots. Some fish have a few, very small, black pigment dots.</p> <p> <b>Description of non-type material.</b> Two specimens from River Kashaf share with holotype and the paratypes most diagnostic features. Thus, they have a completely scaleless sharp ventral keel, –4.2 teeth on the right 5th ceratobranchial, an upturned mouth, 8½ branched dorsal-fin rays, 13½ and 14½ anal-fin branched rays, 41 (20+21) total vertebrae and 13 predorsal abdominal vertebrae (Fig. 2)</p> <p> <b>Etymology.</b> The new species is named for the late Juraj Holcík, Bratislava, colleague and friend, in recognition of his many contributions to the science of ichthyology.</p> <p> <b>Distribution and habitat.</b> The new species is only known from historical collections in the Hari (= Tedzhen) River of Afghanistan and its western tributary from Iran, the Kashaf River (Fig. 3). The Hari River or Harirud is known as the Tedzhen River in Turkmenistan where it is lost in the sands of the Kara Kum desert. The Hari River in Afghanistan dried up completely in 2000 (Payvand News at www.payvand.com/news/ 01/jan/1011.html) and is usually dry from August to November as water is diverted for irrigation and precipitation is low. The city of Mashhad on the Kashaf River in Iran has a population of about 2.5 million people with a concomitant draw on water resources. Water quality in the Kashaf River at Mashhad is degraded (Najafpoor <i>et al</i>., 2007). These shallow rivers with little riparian vegetation in a desert climate have habitats that are stressful for fishes.</p> <p> <b>Comparative remarks.</b> <i>Alburnoides holciki</i> clearly differs from Iranian species <i>A. petrubanarescui</i> Bogutskaya & Coad, 2009, <i>A. namaki</i> Bogutskaya & Coad, 2009, <i>A. nicolausi</i> Bogutskaya & Coad, 2009 and <i>A. idignensis</i> Bogutskaya & Coad, 2009 distributed in the south of the Caspian Sea basin by having a short, slightly pointed snout (vs. stout and rounded), a terminal mouth with the tip of the mouth cleft on a level with the upper half of the pupil (vs. almost or completely subterminal with a tip of the mouth on a level with the lower margin of the pupil to below the lower margin of the eye), a clearly forked caudal-fin with pointed lobes (vs. shallowly forked with rounded lobes), and 13–16½, usually 14–15½, branched anal-fin rays (vs. 8–13½, usually 9–12½), and orbit width about equal to interorbital width (vs. smaller). The new species is further easily distinguishable from <i>A. petrubanarescui</i> in having a sharp, usually scaleless ventral keel (vs. smooth and completely scaled) and a caudal vertebral region longer than an abdominal region: most frequent vertebral formulae 20+21, 20+22 and 19+21 (vs. shorter, usually 21+19 and 21+20), from <i>A. nicolausi</i> – in having usually 8½ branched anal-fin rays (vs. usually 7½), and from both <i>A. nicolausi</i> and <i>A. idignensis –</i> in having 40–42, usually 41, total vertebrae and 13 or 14 predorsal vertebrae (vs. 37–40, usually 39, and 11–13, usually 12, respectively).</p> <p> <i>Alburnoides holciki</i> and <i>A. namaki</i> are similar in having a sharp scaleless ventral keel but <i>A. holciki</i> is clearly different from the latter species in having a pointed snout and a terminal mouth with the tip of the mouth cleft on a level with the upper half of the pupil (vs. a stout markedly rounded snout, a subterminal mouth with the tip of the mouth cleft on a level below the lower margin of the eye), 40–42, usually 41, total vertebrae (vs. 39–41, usually 40), 13 or 14 predorsal vertebrae (vs. usually 12), and 13–16½, usually 14–15½, branched anal-fin rays (vs. 10–13½, usually 11–12½). The new species resembles <i>Alburnoides qanati</i> Coad & Bogutskaya, 2009 in having a terminal upturned mouth but in the latter species the mouth cleft is even more vertical and the tip of the mouth cleft is on about a level with the upper margin of the pupil. <i>Alburnoides holciki</i> differs from <i>A. qanati</i> in having a sharp scaleless ventral keel (vs. smooth and scaled), 13–16½ branched anal-fin rays (vs. 10–12½).</p> <p> <i>Alburnoides</i> from the Caspian and Aral basins (including presently endorheic drainages such as Murghab, Hari and rivers of the Kopetdag Mountains) contain at least two species described up to now (the third one is being described in this paper), <i>A. eichwaldii</i> (De Filippi, 1863) and <i>A. varentsovi</i> Bogutskaya & Coad, 2009. <i>Alburnoides eichwaldii</i> was originally described from the "Kur presso Tiflis" (= Kura River near Tbilisi, now Georgia), so an <i>Alburnoides</i> from Kura River drainage is a bearer of the name; there is another nominal subspecies in the Aras River (a tributary of the Kura) system of Armenia, <i>Alburnoides bipunctatus armeniensis</i> Dadikyan, 1972, from the rivers Arpa, Vorotan, Vedi, Marmarik, Kasakh and their tributaries, now regarded as a synonym of <i>eichwaldii</i> (Bogutskaya & Coad 2009). Bogutskaya & Coad (2009) made an assumption that riffle minnows from the Safid River drainage and the Amu Darya drainage may represent two distinct species. These issues are outside the goal of this paper; for comparisons that confirm the species status of <i>A. holciki</i> we subdivided the examined samples besides those of <i>A. holciki</i> into seven groups: 1. <i>A. eichwaldii</i> from Kura-Aras and Lenkoran (in the north from the Talysh Mountains), 2. <i>Alburnoides</i> cf. <i>eichwaldii</i> from rivers in the south from the Talysh Mountains and west from the Safid, 3. <i>Alburnoides</i> cf. <i>eichwaldii</i> from the Safid River drainage, 4. <i>Alburnoides</i> sp. from the Tajan River (in the east of the Safid), 5. <i>Alburnoides</i> sp. from the Atrek River drainage, 6. <i>A. varentsovi</i> from rivers of the northern slope of Kopetdag, 7. <i>Alburnoides</i> sp. from the Amu Darya drainage (Tables 2–4, Fig. 3). A UPGMA dendrogram using Kullback-Leibler divergences (Fig. 4) show the grouping of these units based on six meristic characters (the number of branched anal-fin rays and five vertebral counts).</p> <p> <i>Alburnoides holciki</i> is most similar to <i>Alburnoides</i> sp. from the Amu Darya drainage (Fig. 5) by the vertebral counts (Tables 3, 4), the presence of a sharp, scaleless ventral keel, 6–8 gill rakers, and 4 pharyngeal teeth in the long row on the 5th ceratobranchial, but differs from it in having 13–16½, usually 14–15½, branched anal-fin rays (vs. 12–14½, usually 13½), and 46–51(55) lateral-line scales to posterior margin of hypurals (vs. 43–47 in examined Amu Darya samples).</p> <p> <i>Alburnoides holciki</i> is also similar to the Atrek riffle minnow in having a sharp, scaleless keel, an upturned, terminal mouth (Fig. 6), and 46–51(55) lateral-line scales to posterior margin of hypurals (45–51 in the Atrek samples). However, the new species differs from the Atrek fish in usually having 4 pharyngeal teeth in the long row on the 5th ceratobranchial (vs. 5); 14–15½ branched anal-fin rays (vs. a mode of 13½); 6–8 gill rakers (vs. 9–10); 40–42, usually 41, total vertebrae (vs. 40); (12)13–14 predorsal vertebrae (vs. 12–13 with a mode of 12); a longer caudal vertebral region, containing (20)21–22 vertebrae (vs. 20–21 with a mode of 20); and 20+21 most usual vertebral formula (vs. 20+20). <i>Alburnoides</i> sp. from the Atrek drainage is morphologically very close to the riffle minnow from the Tajan River by anal-fin and vertebral counts (Fig. 4, Tables 2–4), the number of lateral-line scales to posterior margin of hypurals (45–51 in the Atrek samples and 46–53 in the Tajan sample), but differs by the shape of the mouth which is upturned with a pointed snout and a tip of the mouth cleft on a level with the upper half of the pupil (Fig. 6) (vs. a more horizontal, slightly curved mouth with a rounded snout and a tip of the mouth on a level with the lower margin of the pupil, Fig. 7), a sharp and commonly scaleless ventral keel (vs. sharp but variably scaled), and 5 pharyngeal teeth in the long row on the 5th ceratobranchial (vs. usually 4). This is why we do not consider these samples as conspecific though the Tajan and the Atrek are geographically very close flowing into the south-eastern Caspian Sea.</p> <p> The new species is morphologically close to another geographically close species, <i>A. varentsovi</i> Bogutskaya & Coad, 2009, in having a large eye (the orbit diameter larger than the snout length and about equal to the interorbital width), a triangular-shaped head, and a clearly forked caudal-fin with moderately pointed lobes. But <i>A. holciki</i> is distinguishable from the latter species in having neither lower jaw nor upper jaw protruding relative to each other (vs. a lower jaw slightly protruding over the upper jaw), a sharp scaleless ventral keel (vs. smooth and partly scaleless), most often 2.5–4.2 pharyngeal teeth (vs. commonly 2.5–5.2), 13–16½, usually 14–15½, branched anal-fin rays (vs. 10–14½, usually 12–13½), 40–42, usually 41, total vertebrae (vs. 39–41(42) with a mode of 40), 12–14, with a mode of 13, predorsal vertebrae (vs. 11–13, with a mode of 12), and a caudal vertebral region longer than the abdominal region, most frequent vertebral formulae 20+21, 20+22 and 19+21 (vs. usually equal, 20+20) (Table 2–4).</p> <p> <i>Alburnoides holciki</i> is distinguishable from <i>A. eichwaldii</i> and <i>Alburnoides</i> sp. from rivers in the south of the Talysh Mountains and the Safid River drainage (Fig. 8) in having an upturned mouth with a tip of the mouth cleft on a level with the upper half of the pupil (vs. a more horizontal, slightly curved or straight but never upturned mouth, with a tip of the mouth cleft on a level with the middle of the eye or below), a pointed snout with a straight upper profile (vs. slightly to markedly rounded), a sharp scaleless keel (vs. smooth and variably scaled, often completely scaled), and usually 14–15½ branched anal-fin rays (vs. usually 12½ or 13½) Vertebral counts are summarised in Tables 3–4.</p>Published as part of &lt

Phoxinus undetermined

Phoxinus sp. Kuban’, Clade 19 UKRAINE • 6 specs, 42.2–48.7 mm SL; Adagum River at Krymsk, Kuban’ drainage; 44°54′03″ N, 37°58′31″ E; 23 Jul. 2001; N. Bogutskaya, A. Naseka and J. Freyhof leg.; ZFMK 79044–49. Genetic analysis A single haplotype was detected in all five examined Crimean specimens (Supp. file 2), previously recognised as genetic clade 20 from the Salhir drainage (Palandačić et al. 2017, 2020). From the CO1 mitochondrial gene sequences, this haplotype forms its own haplogroup in the network (Fig. 2) and is 33–97 mutational steps distant from mitochondrial clades analysed in this study, supporting its distinctiveness. Pairwise distances between P. chrysoprasius and the other clades or species range between 4% and 7% (Supp. file 5). Statistical morphological analysis Samples were first compared using only non-morphometric characters to exclude the influence of sexual dimorphism and be able to include the P. marsilii samples for which we did not have a complete set of morphometric data because of variable preservation condition of the specimens. The number of specimens per sample with SL> 45 mm that are sub-adult and adult (314 specimens in total), and a list of characters (11 counts and 2 coded characters represented by 97 character states) are reported in Supp. file 4. DFA (Fig. 3) did not reveal a clear differentiation of the groups. We found that only 63.7% of the specimens were classified correctly. However, the Crimean specimens were differentiated at a relatively high level with 26 out of 29 specimens correctly classified. Characters that contributed most to the discrimination between the groups were the relative length of the first uninterrupted section of the lateral line, the total number of vertebrae and the numbers of abdominal and caudal vertebrae. DFA was then performed using all characters (meristic, coded and morphometric) (Fig. 4) in the samples geographically closest to the Black Sea (Bulgarian P. strandjae, Turkish P. strandjae, P. colchicus and Crimean) for females and males separately, as the species exhibits considerable sexual dimorphism as described below. All specimens were classified successfully (100%), and both males and females of P. chrysoprasius were clearly distant from the respective subsamples of P. colchicus and P. strandjae (Fig. 4A). Total number of lateral-line scales contributed most to the discrimination between samples, followed by the caudal-peduncle length (% SL), caudal-peduncle depth (both % of caudal-peduncle length and % SL), prepelvic length (% SL) and total number of scales in the lateral series. As some of the result might be influenced by differences in the number of females and males (only two males in Turkish P. strandjae and absent in P. colchicus), we undertook an analysis for females only (Fig. 4B). All female specimens were 100% classified. The subsample of Crimean females was almost equidistant from P. colchicus and Bulgarian P. strandjae females (squared Mahalanobis distance 568.445 and 609.09, respectively), but less so (419.83) from the Turkish P. strandjae subsample. In combination, CO1 and the morphological data of the present study strongly support the validity of the native Crimean Phoxinus under the earliest available name of the species: P. chrysoprasius.Published as part of Bogutskaya, Nina G., Diripasko, Oleg A. & Palandačić, Anja, 2023, Novel data support validity of Phoxinus chrysoprasius (Pallas, 1814) (Actinopterygii, Leuciscidae), pp. 1-20 in European Journal of Taxonomy 861 on pages 6-8, DOI: 10.5852/ejt.2023.861.2061, http://zenodo.org/record/771029

Phoxinus chrysoprasius

Phoxinus chrysoprasius, Clade 20 UKRAINE • 17 specs, 49.5–87.2 mm SL; at Krasna Sloboda village, upper reaches of Kuchuk-Karasu, Salhir drainage; 44°58′26″ N, 34°44′19″ E; 16 Jun. 2003; N. Bogutskaya, O. Diripasko, A. Naseka and J. Freyhof leg.; ZFMK 93640–59 * • 12 specs, 54.3–73.7 mm SL; River Salhir upstream of Simferopol Reserve, at Pereval'ne; 44°51′12″ N, 34°18′47″ E; 17 Jun. 2003; N. Bogutskaya, O. Diripasko, A. Naseka and J. Freyhof leg.; ZFMK 93921–30.Published as part of Bogutskaya, Nina G., Diripasko, Oleg A. & Palandačić, Anja, 2023, Novel data support validity of Phoxinus chrysoprasius (Pallas, 1814) (Actinopterygii, Leuciscidae), pp. 1-20 in European Journal of Taxonomy 861 on page 5, DOI: 10.5852/ejt.2023.861.2061, http://zenodo.org/record/771029

Phoxinus colchicus Berg 1910

P. colchicus, Clade 18 GEORGIA • Ilia State University, Institute of Zoology, Tbilisi, no catalogue numbers • 2 ♂♂, SL 32.9– 43.6 mm, 1 ♀, SL 76.5 mm; Kintrishi River; 41°48′13″ N, 41°46′42″ E; Jul. 2018; B. Japoshvili leg. • 4 specs, SL 30.6–34.8 mm, 1 ♀, SL 59.3 mm; Natanebi River, Georgia, 41°55′35″ N, 41°58′2″ E; Aug. 2016; B. Japoshvili leg.Published as part of Bogutskaya, Nina G., Diripasko, Oleg A. & Palandačić, Anja, 2023, Novel data support validity of Phoxinus chrysoprasius (Pallas, 1814) (Actinopterygii, Leuciscidae), pp. 1-20 in European Journal of Taxonomy 861 on page 6, DOI: 10.5852/ejt.2023.861.2061, http://zenodo.org/record/771029

Novel data support validity of Phoxinus chrysoprasius (Pallas, 1814) (Actinopterygii, Leuciscidae)

The common minnow species Cyprinus chrysoprasius, previously synonymised to Phoxinus phoxinus, was originally described from the Crimean Peninsula (Black Sea – Sea of Azov basin). A genetic analysis of the mitochondrial cytochrome oxydase 1 in the context of a phylogenetic study of European Phoxinus showed that it represents a distinct genetic clade and potentially a valid species. In the present study, we approach the issue following a broader, both genetic and morphological, study in order to check and support the validity of native Crimean Phoxinus under the earliest available name of the species: P. chrysoprasius. Our data demonstrate a reliable genetic distance of this minnow from geographically neighbouring clades and species, and a certain morphological distinctiveness. In order to determine the taxonomic concept of P. chrysoprasius, as a species involved in a genetically well-differentiated, but phenotypically poorly structured complex of east-European Phoxinus, a neotype for the species, based on topotypical material, is herein described and designated. The original type locality of the species is also clarified