Data simulation for the Lightning Imaging Sensor (LIS)

This project aims to build a data analysis system that will utilize existing video tape scenes of lightning as viewed from space. The resultant data will be used for the design and development of the Lightning Imaging Sensor (LIS) software and algorithm analysis. The desire for statistically significant metrics implies that a large data set needs to be analyzed. Before 1990 the quality and quantity of video was insufficient to build a usable data set. At this point in time, there is usable data from missions STS-34, STS-32, STS-31, STS-41, STS-37, and STS-39. During the summer of 1990, a manual analysis system was developed to demonstrate that the video analysis is feasible and to identify techniques to deduce information that was not directly available. Because the closed circuit television system used on the space shuttle was intended for documentary TV, the current value of the camera focal length and pointing orientation, which are needed for photoanalysis, are not included in the system data. A large effort was needed to discover ancillary data sources as well as develop indirect methods to estimate the necessary parameters. Any data system coping with full motion video faces an enormous bottleneck produced by the large data production rate and the need to move and store the digitized images. The manual system bypassed the video digitizing bottleneck by using a genlock to superimpose pixel coordinates on full motion video. Because the data set had to be obtained point by point by a human operating a computer mouse, the data output rate was small. The loan and subsequent acquisition of a Abekas digital frame store with a real time digitizer moved the bottleneck from data acquisition to a problem of data transfer and storage. The semi-automated analysis procedure was developed using existing equipment and is described. A fully automated system is described in the hope that the components may come on the market at reasonable prices in the next few years

Lightning observations from space shuttle

The experimental program of the Earth Sciences and Applications Division at NASA/MSFC includes development of the Lightning Imaging Sensor (LIS) for the NOAA Earth Observing System (EOS) Polar Platform. The research plan is to use existing lightning information to generate simulated data for the LIS experiment. Navigation algorithms were used to transform pixel locations to latitude and longitude values. The simulated data would then be used to test and develop algorithms for the analysis of LIS data. Individual frames of video imagery obtained from Space Shuttle Missions provide the raw data for the simulation. Individual video frames were digitized to get the pixel locations of lightning flashes. The pixel locations will be used to locate the geographical position of the event. Because of a lack of detailed knowledge of camera orientation with respect to the Space Shuttle, video scenes that contain identifiable city lights were chosen for analysis. A method for locating the payload bay camera axis was developed and tested. Two measurements are needed: the pixel location of the apparent horizon and a timed siting of a known location passing the principal line of the image. Individual video frames were navigated and lightning illuminated clouds were located on the map. Satisfactory agreement in location was achieved for cities and LLP lightning locations. Ground truth measurements were compared to satellite observations. A vertical lightning event was identified on the horizon. Very low frequency (VLF) transmission on this particular occassion shows a strong response to negative cloud to cloud flashes

Comparative analysis of the transcriptome across distant species

The transcriptome is the readout of the genome. Identifying common features in it across distant species can reveal fundamental principles. To this end, the ENCODE and modENCODE consortia have generated large amounts of matched RNA-sequencing data for human, worm and fly. Uniform processing and comprehensive annotation of these data allow comparison across metazoan phyla, extending beyond earlier within-phylum transcriptome comparisons and revealing ancient, conserved features. Specifically, we discover co-expression modules shared across animals, many of which are enriched in developmental genes. Moreover, we use expression patterns to align the stages in worm and fly development and find a novel pairing between worm embryo and fly pupae, in addition to the embryo-to-embryo and larvae-to-larvae pairings. Furthermore, we find that the extent of non-canonical, non-coding transcription is similar in each organism, per base pair. Finally, we find in all three organisms that the gene-expression levels, both coding and non-coding, can be quantitatively predicted from chromatin features at the promoter using a 'universal model' based on a single set of organism-independent parameters

CFTR modulator theratyping : Current status, gaps and future directions

Background: New drugs that improve the function of the cystic fibrosis transmembrane conductance regulator (CFTR) protein with discreet disease-causing variants have been successfully developed for cystic fibrosis (CF) patients. Preclinical model systems have played a critical role in this process, and have the potential to inform researchers and CF healthcare providers regarding the nature of defects in rare CFTR variants, and to potentially support use of modulator therapies in new populations. Methods: The Cystic Fibrosis Foundation (CFF) assembled a workshop of international experts to discuss the use of preclinical model systems to examine the nature of CF-causing variants in CFTR and the role of in vitro CFTR modulator testing to inform in vivo modulator use. The theme of the workshop was centered on CFTR theratyping, a term that encompasses the use of CFTR modulators to define defects in CFTR in vitro, with application to both common and rare CFTR variants. Results: Several preclinical model systems were identified in various stages of maturity, ranging from the expression of CFTR variant cDNA in stable cell lines to examination of cells derived from CF patients, including the gastrointestinal tract, the respiratory tree, and the blood. Common themes included the ongoing need for standardization, validation, and defining the predictive capacity of data derived from model systems to estimate clinical outcomes from modulator-treated CF patients. Conclusions: CFTR modulator theratyping is a novel and rapidly evolving field that has the potential to identify rare CFTR variants that are responsive to approved drugs or drugs in development

Bayesian estimation of multinomial processing tree models with heterogeneity in participants and items

Multinomial processing tree (MPT) models are theoretically motivated stochastic models for the analysis of categorical data. Here we focus on a crossed-random effects extension of the Bayesian latent-trait pair-clustering MPT model. Our approach assumes that participant and item effects combine additively on the probit scale and postulates (multivariate) normal distributions for the random effects. We provide a WinBUGS implementation of the crossed-random effects pair-clustering model and an application to novel experimental data. The present approach may be adapted to handle other MPT models