What is the best method for preserving the genuine black truffle (Tuber melanosporum) aroma? An olfactometric and sensory approach

The aim of this work was to evaluate the effects of different preservation methods (freeze-drying, hot-air drying, freezing and canning) on the aroma profile of T. melanosporum truffles. Volatile organic compounds (VOCs) were extracted by solid-phase microextraction (SPME) and analysed by gas-chromatography olfactometry to monitor changes occurring in key-aroma compounds. Samples were also submitted to descriptive sensory analysis by a panel of trained judges, with the aim of correlating both sets of data. Freeze-drying - and to a lesser extent hot-air drying - were the only treatments able to retain key-compounds such as dimethylsulphide (DMS) and dimethyldisulphide (DMDS), evoking the aroma typically associated with fresh truffle. Principal component analysis (PCA) performed on the descriptive data showed the sensorial proximity between fresh and freeze-dried truffle, and also the differences between them and those frozen and canned. Despite some differences in the odour volatile profile of fresh and freezed-dried truffles (mainly the lack of 2, 3-butanedione and branched ethyl esters), freeze-drying is the most suitable technique for preserving the overall original aroma of fresh truffle. Several key-odour compounds - mainly unsaturated linear chain carbonyl compounds, sulphur and pyrrole derivates - emerge as biomarkers of the studied technologies

Potential of a new strain of Bacillus amyloliquefaciens BUZ-14 as a biocontrol agent of postharvest fruit diseases

The biocontrol potential of the Bacillus amyloliquefaciens strain BUZ-14 was tested against the main postharvest diseases of orange, apple, grape and stone fruit. After characterizing the temperature and pH growth curves of strain BUZ-14, its in vitro antifungal activity was determined against Botrytis cinerea, Monilinia fructicola, M. laxa, Penicillium digitatum, P. expansum and P. italicum. Subsequently, in vivo activity was tested against these pathogens by treating fruit with cells, endospores and cell-free supernatants. The in vitro results showed that BUZ-14 inhibited the growth of all the pathogens tested corresponding to the least susceptible species, P. italicum, and the most susceptible, M. laxa. In vivo tests corroborated these results as most of the treatments decreased the incidence of brown rot in stone fruit from 100% to 0%, establishing 107 CFU mL-1 as the minimum inhibitory concentration. For the Penicillium species a preventive treatment inhibited P. digitatum and P. italicum growth in oranges and reduced P. expansum incidence in apples from 100% to 20%. Finally, it has been demonstrated that BUZ-14 was able to survive and to control brown rot in peaches stored at cool temperatures, making it a very suitable biocontrol agent for application during the post-harvest storage and marketing of horticultural products

Aroma composition of commercial truffle flavoured oils: does it really smell like truffle?

The present study analyzes the aromatic and odour volatile profiles of truffle flavoured oils commercialized as “black truffle oil”. The aim of this work is twofold: to define the sensory space associated to these products and to explore the possible fraudulent use of artificial flavouring agents not properly identified on the label. For this purpose, 12 commercial truffle flavoured oils available in the Spanish market were submitted to descriptive sensory analysis by a trained panel. The three oils presenting the most interesting profile (in terms of odour nature and/or complexity) were also analyzed by olfactometric analysis, in order to identify the chemical compounds responsible on their aroma. The correlation of sensory and olfactometric data made it possible to understand some of the sensory differences observed among samples, as well as to identify irregularities with respect to the ingredients labelling of some of the studied samples

GCIMS: An R package for untargeted gas chromatography - Ion mobility spectrometry data processing

Gas-Chromatography coupled to Ion Mobility Spectrometry (GC-IMS) based metabolomics is an emerging technique for obtaining fast, reliable untargeted metabolic fingerprints of biofluids. The generated raw data is highly dimensional and complex, suffers from baseline problems, misalignments, long peak tails and strong non-linearities that must be corrected to extract chemically relevant features from samples. In this work, we present our GCIMS R package, which includes spectra loading, metadata handling, denoising, baseline correction, spectral and chromatographic alignment, peak detection, integration, and peak clustering to produce a peak table ready for multivariate data analysis. We discuss package design decisions, and, for illustration purposes, we show a case study of sex discrimination on the basis of the volatile compounds in urine samples. The GCIMS package provides a user-friendly workflow for non-code developers to process their raw data samples

Completing Natural Inflation

If the inflaton is a pseudo-scalar axion, the axion shift symmetry can protect the flatness of its potential from too large radiative corrections. This possibility, known as natural inflation, requires an axion scale which is greater than the (reduced) Planck scale. It is unclear whether such a high value is compatible with an effective field theoretical description, and if the global axionic symmetry survives quantum gravity effects. We propose a mechanism which provides an effective large axion scale, although the original one is sub-Planckian. The mechanism is based on the presence of two axions, with a potential provided by two anomalous gauge groups. The effective large axion scale is due to an almost exact symmetry between the couplings of the axions to the anomalous groups. We also comment on a possible implementation in heterotic string theory.Comment: 9 pages, 1 figur

On the origin of the Galactic thin and thick discs, their abundance gradients and the diagnostic potential of their abundance ratios

Using a semi-analytical model of the evolution of the Milky Way, we show how secular evolution can create distinct overdensities in the phase space of various properties (e.g. age vs metallicity or abundance ratios vs age) corresponding to the thin and thick discs. In particular, we show how key properties of the Solar vicinity can be obtained by secular evolution, with no need for external or special events, like galaxy mergers or paucity in star formation. This concerns the long established double-branch behaviour of [alpha/Fe] vs metallicity and the recently found non-monotonic evolution of the stellar abundance gradient, evaluated at the birth radii of stars. We extend the discussion to other abundance ratios and we suggest a classification scheme, based on the nature of the corresponding yields (primary vs secondary or odd elements) and on the lifetimes of their sources (short-lived vs long-lived ones). The latter property is critical in determining the single- or double- branch behavior of an elementary abundance ratio in the Solar neighborhood. We underline the high diagnostic potential of this finding, which can help to separate clearly elements with sources evolving on different timescales and help determining the site of e.g. the r-process(es). We define the "abundance distance" between the thin and thick disc sequences as an important element for such a separation. We also show how the inside-out evolution of the Milky Way disc leads rather to a single-branch behavior in other disc regions.Comment: 20 pages, 16 figures, to appear in MNRA

Local failure after primary radiotherapy in lung cancer: Is there a role for SBRT?

AimOur purpose is to construe the role of stereotactic body radiation therapy (SBRT) in the management of lung cancer from our early experience with SBRT for salvage treatment in patients with recurrent lung cancer after initial radiation therapy.BackgroundLocoregional recurrences are a frequent challenge in patients treated with radio-chemotherapy for locally advanced NSCLC. Conventional external beam radiation therapy (EBRT) is rarely given as salvage treatment because of the risk of toxicity. There is a paucity of published studies evaluating the role of SBRT in this clinical setting.Materials and methodsBetween 2008 and present, 10 patients with biopsy proven non-small cell lung cancer (NSCLC) underwent 14 radiosurgical procedures for salvage therapy after failing initial radiation treatment. Patients’ age ranged from 54 to 88 years with a median of 74 years in 6 males and 4 females. Intervals from initial radiation treatment to salvage SBRT were 3–33 months with a median of 13 months. SBRT treatments were delivered using Intensity Modulated Volumetric Arc Therapy (VMAT). All patients received concomitant chemotherapy.ResultsOverall survival after salvage radiosurgery ranged from 6 to 41 months (mean 20 months, median 18 months). Four of the ten patients are alive with disease locally controlled. Of the remaining 6 patients, 4 had distant progression of disease with brain metastases and one had both brain and lung metastases. The other patient had a regional failure. Toxicities were found in three of the ten (30%) patients with grade I pneumonitis.ConclusionIn our early experience, salvage SBRT is an effective modality of treating patients who failed after conventional irradiation, achieving excellent results in terms of local control with acceptable toxicity. Further prospective studies are needed to determine optimal fractionation schemes

Chemical and kinematical properties of BSSs and HB stars in NGC 6397

We used three sets of high-resolution spectra acquired with the multifiber facility FLAMES at the Very Large Telescope of the European Southern Observatory to investigate the chemical and kinematical properties of a sample of 42 horizontal branch (HB) stars, 18 Blue Straggler Stars (BSSs) and 86 main sequence turn-off and sub-giant branch stars in the nearby globular cluster NGC 6397. We measured rotational velocities and Fe, O and Mg abundances. All the unevolved stars in our sample turn out to have low rotational velocites (v sin i< 10\kms), while HB stars and BSSs show a broad distribution, with values ranging from 0 to 70 \kms. For HB stars with T<10500 K there is a clear temperature-oxygen anti-correlation, that can be understood if the star position along the HB is mainly determined by the He content. The hottest BSSs and HB stars (with temperatures T>8200 K and T> 10500 K, respectively) also show significant deviations in their iron abundance with respect to the cluster metallicity (as traced by the unevolved stars, [Fe/H]=-2.12). While similar chemical patterns have been already observed in other hot HB stars, this is the first evidence ever collected for BSSs. We interprete these abundance anomalies as due to the metal radiative levitation, occurring in stars with shallow or no convective envelopes

Radio source calibration for the VSA and other CMB instruments at around 30 GHz

Accurate calibration of data is essential for the current generation of CMB experiments. Using data from the Very Small Array (VSA), we describe procedures which will lead to an accuracy of 1 percent or better for experiments such as the VSA and CBI. Particular attention is paid to the stability of the receiver systems, the quality of the site and frequent observations of reference sources. At 30 GHz the careful correction for atmospheric emission and absorption is shown to be essential for achieving 1 percent precision. The sources for which a 1 percent relative flux density calibration was achieved included Cas A, Cyg A, Tau A and NGC7027 and the planets Venus, Jupiter and Saturn. A flux density, or brightness temperature in the case of the planets, was derived at 33 GHz relative to Jupiter which was adopted as the fundamental calibrator. A spectral index at ~30 GHz is given for each. Cas A,Tau A, NGC7027 and Venus were examined for variability. Cas A was found to be decreasing at $0.394 \pm 0.019$ percent per year over the period March 2001 to August 2004. In the same period Tau A was decreasing at $0.22\pm 0.07$ percent per year. A survey of the published data showed that the planetary nebula NGC7027 decreased at $0.16\pm 0.04$ percent per year over the period 1967 to 2003. Venus showed an insignificant ($1.5 \pm 1.3$ percent) variation with Venusian illumination. The integrated polarization of Tau A at 33 GHz was found to be $7.8\pm 0.6$ percent at pa $= 148^\circ \pm 3^\circ$.}Comment: 13 pages, 15 figures, submitted to MNRA

The cut-out phenomenon in intertrochanteric femur fracture: analysis using a finite element model

Trabajo premiado con una beca FEIOMM de Investigación Básica 2018.Objetive: This work aimed to analyze the cut‐out phenomenon, which involves oblique displacements and/or rotations of the femoral head around the cephalic component of the intramedullary nail. The analysis was carried out using finite element numerical models. This technique seeks to understand the failure of this type of fixation and establish what po‐sitioning of the system favors or prevents failure due to cut‐out. Material and methods: The study was carried out on a numerical model of the proximal limb of an artificial femur and an intramedullary nail type PFNA (proximal femoral nail anti‐rotation). In the numerical model, the position of the in‐tramedullary nail was varied in the anterior/posterior and superior/inferior directions to analyze the influence of the position on the cut‐out phenomenon. Stresses in critical areas and torque on the nail under normal position loading were analyzed. Results: The most critical position was the one in which the intramedullary nail is placed in the superior position, due to the high compressions that appear in the trabecular bone of the femoral head. The centered position of the nail de‐creased the risk of bone damage and the torque that the intramedullary nail has to support. Conclusions: This type of model allows us to simulate the influence of the nail position and obtain variables that are otherwise difficult to analyze. Although it is a simple model with static load, it confirms that a centered position of the intramedullary nail reduces the risk of cut‐out .Objetivo: El objetivo del trabajo fue analizar el fenómeno de cut-out, fenómeno que supone desplazamientos oblicuos y/o rotaciones de la cabeza femoral alrededor del componente cefálico del clavo intramedular. El análisis se llevó a cabo mediante modelos numéricos de elementos finitos. Con esta técnica se busca entender el fallo de este tipo de fijaciones y establecer qué posicionamiento del sistema favorece o evita el fallo por cut-out. Material y métodos: El estudio se realizó sobre un modelo numérico de la extremidad proximal de un fémur artificial y un clavo intramedular tipo PFNA (femoral proximal de antirrotación). En el modelo numérico se varió la posición del clavo intramedular en dirección anterior/posterior y superior/inferior para analizar la influencia de la posición en el fenómeno de cut-out. Se analizaron las tensiones en zonas críticas y par torsor sobre el clavo bajo una carga en posición normal. Resultados: La posición más crítica fue aquella en la que el clavo intramedular está colocado en la posición superior, debido a las altas compresiones que aparecen en el hueso trabecular de la cabeza femoral. La posición centrada del clavo disminuyó el riesgo de daño óseo y el par torsor que tiene que soportar el clavo intramedular. Conclusión: Este tipo de modelos permite simular la influencia de la posición del clavo y obtener variables que de otra manera son difíciles de analizar. Aunque se trata de un modelo sencillo con carga estática, confirma que una posición centrada del clavo intramedular disminuye el riesgo de cut-out.Los autores agradecen la financiación recibida a través de la Sociedad Española de Investigación Ósea y del Metabolismo Mineral con la Beca de Investigación 2018. También agradecen la financiación recibida a través del Ministerio de Ciencia e Innovación y el Programa FEDER a través de los proyectos DPI2017-89197-C2-1-R y DPI2017-89197-C2-2-R