CASO APLICATIVO DEL SISTEMA DE GESTIÓN DIGITAL: GESTIÓN DE PROYECTOS DE INVESTIGACIÓN

ResumenPara mejorar el desempeño y la eficiencia de procesos administrativos en la Instituciones de Educación Superior (IES), se han creado Sistemas de Gestión Digital (SGD) que apoyen en la ejecución de estos procesos. El SGD desarrollado está basado en una arquitectura institucional [Silva, 2017] que contempla a su vez 4 arquitecturas interrelacionadas. El objetivo de este trabajo fue tener un caso particular que funciona como prueba de concepto, para el diseño de un módulo del SGD, en particular el seguimiento de la gestión de proyectos de investigación. En este caso se aplicó la arquitectura institucional para el desarrollo de los componentes necesarios para la implementación de un módulo del SGD. El módulo está diseñado para automatizar y mejorar los procesos en la gestión de proyectos de investigación para controlar el flujo de información, reducir el tiempo en trámites y delegar responsabilidades al módulo de gestión de proyectos de investigación.Palabras Claves: Arquitectura de procesos, gestión de proyectos de investigación, sistema de gestión digital. APPLICABLE CASE OF THE DIGITAL MANAGEMENT SYSTEM: MANAGEMENT OF RESEARCH PROJECTSAbstractTo improve the performance and efficiency of administrative processes in the Higher Education Institutions (HEI), Digital Management Systems (DMS) have been created to support execution of these processes. DMS developed is based on an institutional architecture [Silva-López, 2017a], [Silva-López, 2017b], [Silva-López, 2017c] which contains 4 interrelated architectures. The objective of this work was to have a particular case that works as a concept proof, for the design a module of the DMS, in particular monitoring of management of research projects. In this case, the institutional architecture was applied for the development of some components of a module of the DMS. Module is designed to automate and improve processes in the management of research projects to control information flow, reduce procedures time and delegate responsibilities to the research projects management system.Keywords: Digital management system, process architecture, research project management

Understanding the indirect DNA read-out specificity of I-CreI Meganuclease

The high DNA specificity of homing endonucleases makes them a powerful protein scaffold to engineer enzymes for genome manipulation. Understanding their molecular recognition of DNA is an important prerequisite to generate engineered enzymes able to cleave DNA in specific desired genome sites. Protein-DNA recognition studies have been mostly focused on specific direct contacts between amino acid side chains and bases to redesign the binding interface. However, the important role of indirect readout in the central region of the target DNA of the homing endonuclease I-CreI suggested that indirect readout may play a key role in the redesign of protein-DNA interactions. The sequences of the I-CreI central substrate region, 2NN, along with the adjacent 5NNN, are key for substrate cleavage. Here, we analyse the mechanism of target discrimination at the 5NNN region by the I-CreI protein, revealing its critical role in the location and occupancy of the catalytic metal ions, which is crucial for cleavage. Our data highlight the importance of indirect readout for target DNA cleavage, thus aiding I-CreI engineering when targeting new DNA sequences.e thank the Swiss Light Source (SLS) and ALBA beamline staff for their support. This work was supported by the Spanish MINECO (JCI-2011-09308 to R.M and CTQ2017-83810-R to F.J.B.), the Severo Ochoa Excellence Accreditation (SEV-2016-0644) and the Novo Nordisk Foundation (Grant NNF14CC0001 to G.M.),S

Effect of thermal processing on the profile of bioactive compounds and antioxidant capacity of fermented orange juice

Previously, we reported that alcoholic fermentation enhanced flavanones and carotenoids content of orange juice. The aim of this work was to evaluate the influence of pasteurization on the qualitative and quantitative profile of bioactive compounds and the antioxidant capacity of fermented orange juice. Ascorbic acid (203 mg/L), total flavanones (647 mg/L), total carotenoids (7.07 mg/L) and provitamin A (90.06 RAEs/L) values of pasteurized orange beverage were lower than those of fermented juice. Total phenolic remained unchanged (585 mg/L) and was similar to that of original juice. The flavanones naringenin-7-O-glucoside, naringenin-7-O-rutinoside, hesperetin-7-O-rutinoside, hesperetin-7-O-glucoside and isosakuranetin-7-O-rutinoside, and the carotenoids karpoxanthin and isomer, neochrome, lutein, ζ-carotene, zeaxanthin, mutatoxanthin epimers, β-cryptoxanthin and auroxanthin epimers were the major compounds. Pasteurization produced a decrease in antioxidant capacity of fermented juice. However, TEAC (5.45 mM) and ORAC (6353 μM) values of orange beverage were similar to those of original orange juice. The novel orange beverage could be a valuable source of bioactive compounds with antioxidant capacity and exert potential beneficial effects.We are grateful for the support of Junta de Andalucía (Projects P09-AGR4814M, P08-AGR-03477 and Grupo PAI BIO311), and of National Funding Agencies (Projects AGL2010-14850/ALI, AGL2011-23690, CSD007-0063 and CSIC 201170E041). We are also grateful to Fundación Séneca - CARM “Group of Excellence in Research” 04486/GERM/06. The research project grant of B.E.-L. is supported by Junta de Andalucía. A.G.-I., F.F. and S.M. are members of the CORNUCOPIA Network 112RT0460 and D.H.-M. of the IBERCAROT Network 112RT0445 financed by CYTED. We are also grateful to Grupo Hespérides Biotech S.L. for providing the samples.Peer Reviewe

Parvovirus Infection Triggering Still’s Disease

We present the case of a 59-year-old man with acute B19 parvovirus infection who developed a systemic inflammatory reaction similar to adult-onset Still's disease (AOSD). We discuss the clinical challenge due to overlapping symptoms to distinguish between a primary B19 viral infection and the autoimmune disease it can trigger

Clinical Utility of Ghrelin-O-Acyltransferase (GOAT) Enzyme as a Diagnostic Tool and Potential Therapeutic Target in Prostate Cancer

Recent data suggested that plasma Ghrelin O-Acyl Transferase enzyme (GOAT) levels could represent a new diagnostic biomarker for prostate cancer (PCa). In this study, we aimed to explore the diagnostic and prognostic/aggressiveness capacity of GOAT in urine, as well as to interrogate its putative pathophysiological role in PCa. We analysed urine/plasma levels of GOAT in a cohort of 993 patients. In vitro (i.e., cell-proliferation) and in vivo (tumor-growth in a xenograft-model) approaches were performed in response to the modulation of GOAT expression/activity in PCa cells. Our results demonstrate that plasma and urine GOAT levels were significantly elevated in PCa patients compared to controls. Remarkably, GOAT significantly outperformed PSA in the diagnosis of PCa and significant PCa in patients with PSA levels ranging from 3 to 10 ng/mL (the so-called PSA grey-zone). Additionally, urine GOAT levels were associated to clinical (e.g., Gleason-score, PSA levels) and molecular (e.g., CDK2/CDK6/CDKN2A expression) aggressiveness parameters. Indeed, GOAT overexpression increased, while its silencing/blockade decreased cell-proliferation in PCa cells. Moreover, xenograft tumors derived from GOAT-overexpressing PCa (DU145) cells were significantly higher than those derived from the mock-overexpressing cells. Altogether, our results demonstrate that GOAT could be used as a diagnostic and aggressiveness marker in urine and a therapeutic target in PCa

Gestión por proyecto: una solución metodológica para lograr la eficiencia en contexto del aprendizaje de la lengua inglesa para la carrera de Estudios Socioculturales de la UNAH.

En el contexto de la enseñanza-aprendizaje de inglés en la Carrera de Estudios Socioculturales de la UNAH, la gestión por proyecto se argumenta como una necesidad metodológica que permita al docente y al estudiante alcanzar la eficiencia en la producción del conocimiento, entendido esto como el logro de la competencia comunicativa. Para mediar en el proceso del logro de esta competencia, se propone una batería de variables cualitativas, conformada por la “coherencia”, es decir, que las normas para el desempeño concuerden con el desempeño; la “pertinencia”, o el mayor valor social posible del proyecto; la “producción científica”, o la socialización del conocimiento resultante; la “relevancia”, o su mayor reconocimiento social posible; y el “impacto”, o la transformación más eficiente del objeto de investigación, en ese orden lógico, consecutivo, de subordinación metodológica. En este sentido, este artículo da a conocer la argumentación que sustenta esta propuesta, la cual debe resultar, como señalamos en el logro de la competencia comunicativa, como criterio del logro de la eficiencia.Eje: Educación universitaria: Experiencias educativasRed de Universidades con Carreras en Informática (RedUNCI