Evaluation of sperm chromatin structure in boar semen

This study was an attempt to evaluate sperm chromatin structure in the semen of insemination boars. Preparations of semen were stained with acridine orange, aniline blue, and chromomycin A3. Abnormal protamination occurred more frequently in young individuals whose sexual development was not yet complete, but may also be an individual trait. This possibility is important to factor into the decision regarding further exploitation of insemination boars. Thus a precise assessment of abnormalities in the protamination process would seem to be expedient as a tool supplementing morphological and molecular evaluation of semen. Disruptions in nucleoprotein structure can be treated as indicators of the biological value of sperm cells

Nutritional Value of Garden Dill (Anethum graveolens L.), Depending on Genotype

The yield, chemical composition and antioxidant activity of eight genotypes (‘Krezus’, ‘Turkus’, ‘Kozak’, ‘Szmaragd’, ‘Lukullus’, ‘Herkules’, ‘Ambrozja’, ‘Moravan’) of garden dill (Anethum graveolens L.) biomass was estimated. Field experiments were conducted in 2011-2013 in Poland. The crop lasted 44 days and plants were collected at the 4-5 true leaf stage (a bunch harvest). The highest marketable yield was obtained for ‘Krezus’ and ‘Szmaragd’ cultivars (1.18 kg·m-2), while the lowest for ‘Herkules’ (0.53 kg·m-2). The dry matter of leaves ranged from 15.17% (‘Ambrozja’) to 19.27% (‘Krezus’). The study also proved that the genotype influenced the content of chlorophylls a+b, e.g. 1.10 g·kg-1 (‘Moravan’) and 0.78 g·kg-1 (‘Lukullus’), respectively carotenoids: 21.43 mg·100 g-1 (‘Moravan’) and 11.78 mg·100 g-1 (‘Szmaragd’). The content of nitrates (371.48 mg·kg-1 ‘Lukullus’ - 110 mg·kg-1 ‘Szmaragd’), K (11.30% ‘Moravan’ - 5.37% ‘Kozak’), Ca (2.06% ‘Turkus’ - 1.77% ‘Kozak’), and oils (99.13% ‘Lukullus’ - 93.82% ‘Ambrozja’) was also varied. In most cases the content of Mg, P, sugars and ascorbic acid was on similar level. The antioxidant activity was significantly different between tested groups (12.22 µM·g-1 ‘Turkus’ - 6.27 µM·g-1 ‘Krezus’). The presented research proved that the genotype of garden dill affects yield and chemical composition of plants

Main influencing factors and health-related quality of life issues in patients with oesophago-gastric cancer : as measured by EORTC tools

AIM OF THE STUDY: To assess influencing factors and main health-related quality of life (HRQoL) issues in patients with cancers of the oesophago-gastric region using the European Organization for Research and Treatment of Cancer (EORTC) quality of life questionnaire Core 30 (QLQ-C30) and its oesophago-gastric module (QLQ-OG25). MATERIAL AND METHODS: Patients were qualified for this study based on the histological confirmation of oesophageal, oesophago-gastric or gastric cancers. Each patient filled out the Polish version of the EORTC QLQ-C30, the QLQ-OG25 module and a personal questionnaire. Patients were divided into groups based on gender, age, treatment intention, tumour localization, working status and level of education. RESULTS: Our study included 112 patients – 39 women (35%) and 73 men (mean age ± SD; 60.2 ±10.9). Thirty-five patients (31.3%) completed the questionnaires twice. Eighty-four (75%) patients had gastric cancer (GC), twenty-six (23.2%) oesophageal cancer (OC) and two (1.8%) cancer of the oesophago-gastric junction (OGJC). Eighty (71.4%) patients underwent surgical treatment prior to either chemo-, radio- or chemoradiotherapy. The Global Health Status scale of the QLQ-C30 inversely correlated with all the other QLQ-C30 and QLQ-OG25 symptom scales (r = –0.26 to –0.61; p < 0.05). CONCLUSIONS: The main HRQoL problems of Polish OC, OGJC and GC patients are fatigue, insomnia, anxiety, and appetite and weight loss. Older age, receiving palliative treatment, having gastric cancer, being on retirement and having lower education are factors associated with higher symptom scores (worse symptoms) and thus poorer HRQoL

Mortality Following Clostridioides difficile Infection in Europe : A Retrospective Multicenter Case-Control Study

We aimed to describe the clinical presentation, treatment, outcome and report on factors associated with mortality over a 90-day period in Clostridioides difficile infection (CDI). Descriptive, univariate, and multivariate regression analyses were performed on data collected in a retrospective case-control study conducted in nine hospitals from seven European countries. A total of 624 patients were included, of which 415 were deceased (cases) and 209 were still alive 90 days after a CDI diagnosis (controls). The most common antibiotics used previously in both groups were β-lactams; previous exposure to fluoroquinolones was significantly (p = 0.0004) greater in deceased patients. Multivariate logistic regression showed that the factors independently related with death during CDI were older age, inadequate CDI therapy, cachexia, malignancy, Charlson Index, long-term care, elevated white blood cell count (WBC), C-reactive protein (CRP), bacteraemia, complications, and cognitive impairment. In addition, older age, higher levels of WBC, neutrophil, CRP or creatinine, the presence of malignancy, cognitive impairment, and complications were strongly correlated with shortening the time from CDI diagnosis to death. CDI prevention should be primarily focused on hospitalised elderly people receiving antibiotics. WBC, neutrophil count, CRP, creatinine, albumin and lactate levels should be tested in every hospitalised patient treated for CDI to assess the risk of a fatal outcome

The Effect of Plant-Derived Biostimulants on White Head Cabbage Seedlings Grown under Controlled Conditions

The aim of this study was to investigate the potential of using natural raw materials that have not been used for the production of biostimulants of plant growth. These products can effectively contribute to overcome the challenge posed by the increasing demand for food. Ultrasound assisted extraction (UAE) was chosen to prepare innovative biostimulants. The expected outcome of this research was to generate products that show beneficial effects on white head cabbage growth, development, and nutritional quality. The results proved that higher plants (mugwort, calendula, purple coneflower, chamomile, basil, giant goldenrod, comfrey, dandelion, and valerian) can be successfully used for the production of biostimulants. For example, products based on common dandelion showed the highest biostimulating activity. In a group treated with 2.5% flower extract, cabbage shoots were 37% longer, whereas in a group treated with 0.5% leaves extract, roots were longer by 76% as compared with a control group treated with water. Biostimulants based on common mugwort (at a dose of 0.5%) and common dandelion (root) (at a dose of 1.0%) increased the mass of cabbage shoots and roots by 106% and 246%, respectively. The majority of biostimulants increased the content of photosynthetic pigments (chlorophyll and carotenoids) and decreased the content of polyphenols. Botanical extracts also showed varied impact on the antioxidant activity of cabbage. Taking into account the benefits for a wide scope of applications, it is expected that the utilization of these types of products should increase in the future. These products can play a key role in sustainable agriculture