Project Khepri: Mining Asteroid Bennu for Water

Deep space asteroid mining presents the opportunity for the collection of critical resources required to establish a cis-lunar infrastructure. In specific, the Project Khepri team has focused on the collection of water from asteroid Bennu. This water has the potential to provide a source of clean-energy propellant as well as an essential consumable for humans or agriculture on crewed trips to the Moon or Mars. This would avoid the high costs of launching from Earth - making it a highly desirable element for the future of cis-lunar infrastructure. The OSIRIS-REx mission provided a complete survey of asteroid Bennu and is set to return regolith samples to Earth in 2023. This makes asteroid Bennu a well-understood and low-risk target that is estimated to be around 6.26% water by mass. The Khepri Project comprises a team of international students, academics, and industry subject matter experts working on the technical design, business case, and political aspects of a mission to mine asteroid Bennu for water. The research output explores the multi-year mission that the Khepri team has proposed

A Novel Approach to Interstellar Object Exploration Using Small Spacecraft

In 2014, the first interstellar object known to mankind hit the Earth and sunk inthe Pacific Ocean. Two more objects were observed shortly after in 2017 and 2019. Interstellar objects are believed to be planetesimals or chunks of rock that were knocked off planetary bodies during the formation and early evolution processes of neighboring solar systems in the Milky Way. As a result, they are believed to contain a great wealth of data that could further our understanding of exoplanets, extra-solar systems and the formation of our solar system. Carrying out a mission to an ISO would enhance our knowledge of near-Earth asteroids (NEAs) and allow us to develop a more effective planetary defense system. This thesis explores different techniques and technologies that would simplify our approach to such endeavor

Harnessing the Power of Metabarcoding in the Ecological Interpretation of Plant-Pollinator DNA Data: Strategies and Consequences of Filtering Approaches

Although DNA metabarcoding of pollen mixtures has been increasingly used in the field of pollination biology, methodological and interpretation issues arise due to its high sensitivity. Filtering or maintaining false positives, contaminants, and rare taxa or molecular features could lead to different ecological results. Here, we reviewed how this choice has been addressed in 43 studies featuring pollen DNA metabarcoding, which highlighted a very high heterogeneity of filtering methods. We assessed how these strategies shaped pollen assemblage composition, species richness, and interaction networks. To do so, we compared four processing methods: unfiltering, filtering with a proportional 1% of sample reads, a fixed threshold of 100 reads, and the ROC approach (Receiver Operator Characteristic). The results indicated that filtering impacted species composition and reduced species richness, with ROC emerging as a conservative approach. Moreover, in contrast to unfiltered networks, filtering decreased network Connectance and Entropy, and it increased Modularity and Connectivity, indicating that using cut-off thresholds better describes interactions. Overall, unfiltering might compromise reliable ecological interpretations, unless a study targets rare species. We discuss the suitability of each filtering type, plead for justifying filtering strategies on biological or methodological bases and for developing shared approaches to make future studies more comparable

APPLICATION OF ELECTROKINETIC PHENOMENA IN: DEWATERING, CONSOLIDATION ANDSTABILIZATION OF SOILS AND WEAK ROCKS IN CIVIL AND PETROLEUM ENGINEERING,AND AUGMENTING RESERVOIR ENERGY DURING PETROLEUM PRODUCTION. - Page 321

The way pollinators gather resources may play a key role for buffering their population declines. Social pollinators like bumblebees could adjust their foraging after significant workforce reductions to keep provisions to the colony optimal, especially in terms of pollen diversity and quantity. To test what effects a workforce reduction causes on the foraging for pollen, commercially-acquired colonies of the bumblebee Bombus terrestris were allowed to forage in the field and they were experimentally manipulated by removing half the number of workers. For each bumblebee, the pollen pellets were taxonomically identified with DNA metabarcoding of the ITS2 region followed by a statistical filtering based on ROC curves to filter out underrepresented OTUs. Video cameras and network analyses were employed to investigate changes in foraging strategies and behaviour. After filtering out the false-positives, HTS metabarcoding yielded a high plant diversity in the pollen pellets; for plant identity and pollen quantity traits no differences emerged between samples from treated and from control colonies, suggesting that plant choice was influenced mainly by external factors such as the plant phenology. The colonies responded to the removal of 50% of their workers by increasing the foraging activity of the remaining workers, while only negligible changes were found in diet breadth and indices describing the structure of the pollen transport network. Therefore, a consistency in the bumblebees' feeding strategies emerges in the short term despite the lowered workforce

Site-selective assembly between 1,8-diiodoperfluorooctane and 4,7,8,11-tetraazahelicene driven by halogen bonding

reserved9The X-ray diffraction analysis on single crystals obtained from the self-assembly of 1,8-diiodoperfluorooctane 1 with the tetraazahelicene derivative 2 revealed a site-selective pattern of halogen bonds (XBs) in the solid state. Indeed, two non-equivalent XBs drive the formation of an indefinitely repeating pentameric (1)3 center dot(2)2 unit. Interestingly, the N...I-C bonding formation occurred selectively on the pyridazinic/cinnolinic nitrogen atoms of the tetraazahelicene unit, which were preferred over the pyridinic/quinolinic ones. On the basis of molecular electrostatic potential and molecular orbital analyses, DFT calculations predicted and explained well this site-selective XB formation, thus demonstrating to be efficient tools for the prediction of the XB site selectivity in similar polynitrogen systems.S. Biella; M. Cametti; T. Caronna; G. Cavallo; A. Forni; P. Metrangolo; T. Pilati; G. Resnati; G. TerraneoBiella, Serena; Cametti, Massimo; T., Caronna; Cavallo, Gabriella; A., Forni; Metrangolo, Pierangelo; T., Pilati; Resnati, Giuseppe; Terraneo, Giancarl

Raw Data Pioltelli et al. <strong>LANDSCAPE FRAGMENTATION CONSTRAINS BUMBLEBEE NUTRITIONAL ECOLOGY AND FORAGING DYNAMICS</strong>

Project that include four datasets with all the raw data used in the study.  The first dataset contains the results of the chemical analyses on the pollen foraged by Bombus terrestris (i.e. macronutrients contents, antioxidants content and total polyphenols and flavonoids content. Data on pollen richness (number of plant species identified in the pollen through DNA metabarcoding analysis) are also reported. The landscape variable computed in a buffer of 1000 m are reported.  The second dataset contains the results of the monitoring of the foraging activity of the colonies. The number of foragers leaving and entering the colony in an interval of 20 minutes are reported. The landscape variable computed in a buffer of 1000 m are reported.  The third dataset contain the weight measurement of the colony along with the date in which the measurement has been performed. The fourth dataset report the results of the DNA metabarcoding analysis. The ESV table indicate the number of reads for each ESV identified in the pollen samples. </p

Mimicking orchids lure bees from afar with exaggerated ultraviolet signals

: Flowers have many traits to appeal to pollinators, including ultraviolet (UV) absorbing markings, which are well-known for attracting bees at close proximity (e.g., <1 m). While striking UV signals have been thought to attract pollinators also from far away, if these signals impact the plant pollinia removal over distance remains unknown. Here, we report the case of the Australian orchid Diuris brumalis, a nonrewarding species, pollinated by bees via mimicry of the rewarding pea plant Daviesia decurrens. When distant from the pea plant, Diuris was hypothesized to enhance pollinator attraction by exaggeratedly mimicking the floral ultraviolet (UV) reflecting patterns of its model. By experimentally modulating floral UV reflectance with a UV screening solution, we quantified the orchid pollinia removal at a variable distance from the model pea plants. We demonstrate that the deceptive orchid Diuris attracts bee pollinators by emphasizing the visual stimuli, which mimic the floral UV signaling of the rewarding model Daviesia. Moreover, the exaggerated UV reflectance of Diuris flowers impacted pollinators' visitation at an optimal distance from Da. decurrens, and the effect decreased when orchids were too close or too far away from the model. Our findings support the hypothesis that salient UV flower signaling plays a functional role in visual floral mimicry, likely exploiting perceptual gaps in bee neural coding, and mediates the plant pollinia removal at much greater spatial scales than previously expected. The ruse works most effectively at an optimal distance of several meters revealing the importance of salient visual stimuli when mimicry is imperfect

Pharmacogenomics in Alzheimer's disease: a genome-wide association study of response to cholinesterase inhibitors

We conducted a genome-wide association study in a cohort of 176 Italian Alzheimer's disease (AD) patients with extreme phenotype of response to cholinesterase inhibitors. Patients were classified into responders in case of positive, stable, or 3 points worsening during a median follow-up of 0.85 years of treatment. Forty-eight single-nucleotide polymorphisms were selected for replication in 198 additional AD-treated patients. By using the dichotomous response trait and a quantitative trait approach (change of mini-mental state examination), a nominal replication and evidence of association when combining data were achieved for 2 single-nucleotide polymorphisms associated with response to treatment: rs6720975(A) (p(combined) = 2.9 x 10(-5), beta regression coefficient: 1.61) and rs17798800(A) (p(combined) = 6.8 x 10(-6), odds ratio = 0.38, 95% confidence interval = 0.25-0.58). Rs6720975 maps in the intronic region of PRKCE, a protein kinase involved in several cellular functions, whereas rs17798800 is intergenic and, according to expression quantitative trait locus (eQTL) analysis, it acts as a cis-regulator of NBEA, an A kinase-anchoring protein playing a substantial role in the maturation of the nervous system. Despite its limitations, this project paves the way for the application of personalized medicine in AD patients and for collaborative efforts in this field. (C) 2013 Elsevier Inc. All rights reserved

Network hyperexcitability within the deep layers of the pilocarpine-treated rat entorhinal cortex

In this study we report that in the presence of normal buffer, epileptiform discharges occur spontaneously (duration = 2.60 ± 0.49 s) or can be induced by electrical stimuli (duration = 2.50 ± 0.62 s) in the entorhinal cortex (EC) of brain slices obtained from pilocarpine-treated rats but not in those from age-matched, nonepileptic control (NEC) animals. These network-driven epileptiform events consist of field oscillatory sequences at frequencies greater than 200 Hz that most often initiate in the lateral EC and propagate to the medial EC with 4–63 ms delays. The NMDA receptor antagonist CPP depresses the rate of occurrence (P < 0.01) of these spontaneous epileptiform discharges but fails in blocking them. Paradoxically, stimulus-induced epileptiform responses are enhanced in duration during CPP application. However, concomitant application of NMDA and non-NMDA glutamatergic antagonists abolishes spontaneous and stimulus-induced epileptiform events. Intracellular recordings from lateral EC layer V cells indicate a lower frequency of spontaneous hyperpolarizing postsynaptic potentials in pilocarpine-treated tissue than in NEC (P < 0.002) both under control conditions and with glutamatergic receptor blockade; the reversal potential of pharmacologically isolated GABAA receptor-mediated inhibitory postsynaptic potentials has similar values in the two types of tissue. Finally, immunohistochemical analysis shows that parvalbumin-positive interneurons are selectively reduced in number in EC deep layers. Collectively, these results indicate that reduced inhibition within the pilocarpine-treated EC layer V may promote network epileptic hyperexcitability