Ronald Reagan a wyzwania epoki

Z wprowadzenia: "Śmierć Ronalda Reagana w czerwcu 2004 r. zamknęła znaczącą epokę w dziejach Europy i świata. Reagan symbolizował pokolenie polityków, którym przyszło działać w czasach szybkiej dekompozycji dwóch, wydawałoby się niepodważalnych pewników politycznych dominujących po drugiej wojnie światowej w świecie zachodnim. Z polityków tych był tym, który przyczynił się w olbrzymiej mierze do podważenia obu z nich."(...

Microwave Non-Destructive Testing for Delamination Detection in Layered Composite Pipelines

Microwave imaging and defectoscopy are promising techniques for dielectric composite evaluation. Their most significant advantage is their relatively high penetration depth. Another feature worth noting is that traditional methods could not acquire an internal content with such a low impact on both the sample and surrounding environment, including the test operator, compared to other techniques. This paper presents microwave non-destructive and noninvasive methods for quality evaluation of layered composite materials using an open-ended waveguide probe. Pure |S11| parameters only exceptionally give a clear answer about the location of material cracks. Therefore, this makes it necessary to analyze these parameters simultaneously along with several other factors, such as stand-off distance, probe type or wave polarization. The purpose of the work was to find the dependency between the physical state of a layered composite powerplant pipeline and the S-matrix parameters response (reflection and transmission parameters) in a Ku frequency band that has not yet been extensively researched. Lower-frequency measurements broaden the application possibility for thicker composites, mainly because of a higher penetration depth and measurement setup availability. Different methods have been shown, including reflection and transmission/reflection methods, both in close proximity and in stand-off distance. The measurements are based on a low-complexity experimental setup

Impact of Activation of <i>EGFL7</i> within Microenvironment of High Grade Ovarian Serous Carcinoma on Infiltration of CD4+ and CD8+ Lymphocytes

Background: It has been demonstrated that Egfl7 promotes tumor cell escape from immunity by downregulating the activation of tumor blood vessels. Aim: to analyze mRNA expression of EGFL7 within the tumor microenvironment of high-grade ovarian serous carcinoma and its association with a number of intraepithelial CD4+/CD8+ lymphocytes and ICAM-1 expression. Methods: qPCR analysis of EGFL7 mRNA in cancer cells and adjacent stromal endothelium microdissected from formalin-fixed paraffin-embedded tumors of 59 high-grade ovarian serous carcinoma patients, was performed. Infiltration of intraepithelial lymphocytes (CD4+/CD8+) and expression of ICAM-1 were evaluated by immunohistochemistry and compared between tumors with different statuses of EGFL7 expression. Results: EGFL7 was expressed in cancer cells (9/59, 15.25%), endothelium (8/59, 13.56%), or both cancer cells and adjacent endothelium (4/59, 6.78%). ICAM-1 was expressed on cancer cells (47/59, 79.66%), stromal endothelium (46/59, 77.97%), or both epithelium and endothelium (40 of 59, 67.8%). EGFL7-positivity of cancer cells and endothelium was associated with lower intraepithelial inflow of CD4+ (p = 0.022 and p = 0.029, respectively) and CD8+ lymphocytes (p = 0.004 and p = 0.031, respectively) but impact neither epithelial nor endothelial ICAM-1 expression (p = 0.098 and p = 0.119, respectively). The patients’ median follow-up was 23.83 months (range 1.07–78.07). Lack of prognostic significance of EGFL7-status and ICAM-1 expression was notified. Conclusion: EGFL7 is activated in the cancer cells as frequently as in the endothelium of human high-grade ovarian serous carcinoma. Activation of EGFL7 in cancer cells and/or endothelial cells could negatively impact diapedesis regardless of localization

Materials characterization of TiO2TiO_2 nanotubes decorated by Au nanoparticles for photoelectrochemical applications

The structural and chemical modification of TiO(2) nanotubes (NTs) by the deposition of a well-controlled Au deposit was investigated using a combination of X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), Scanning Transmission Electron Microscopy (STEM), Raman measurements, UV-Vis spectroscopy and photoelectrochemical investigations. The fabrication of the materials focused on two important factors: the deposition of Au nanoparticles (NPs) in UHV (ultra high vacuum) conditions (1–2 × 10(−8) mbar) on TiO(2) nanotubes (NTs) having a diameter of ∼110 nm, and modifying the electronic interaction between the TiO(2) NTs and Au nanoparticles (NPs) with an average diameter of about 5 nm through the synergistic effects of SMSI (Strong Metal Support Interaction) and LSPR (Local Surface Plasmon Resonance). Due to the formation of unique places in the form of “hot spots”, the proposed nanostructures proved to be photoactive in the UV-Vis range, where a characteristic gold plasmonic peak was observed at a wavelength of 580 nm. The photocurrent density of Au deposited TiO(2) NTs annealed at 650 °C was found to be much greater (14.7 μA cm(−2)) than the corresponding value (∼0.2 μA cm(−2)) for nanotubes in the as-received state. The IPCE (incident photon current efficiency) spectral evidence also indicates an enhancement of the photoconversion of TiO(2) NTs due to Au NP deposition without any significant change in the band gap energy of the titanium dioxide (E(g) ∼3.0 eV). This suggests that a plasmon-induced resonant energy transfer (PRET) was the dominant effect responsible for the photoactivity of the obtained materials

Pathway‑level mutation analysis in primary high‑grade serous ovarian cancer and matched brain metastases

Brain metastases (BMs) in ovarian cancer (OC) are a rare event. BMs occur most frequently in high-grade serous (HGS) OC. The molecular features of BMs in HGSOC are poorly understood. We performed a whole-exome sequencing analysis of ten matched pairs of formalin-fixed paraffin-embedded samples from primary HGSOC and corresponding BMs. Enrichment significance (p value; false discovery rate) was computed using the Reactome, the Kyoto Encyclopedia of Genes and Genomes pathway collections, and the Gene Ontology Biological Processes. Germline DNA damage repair variants were found in seven cases (70%) and involved the BRCA1, BRCA2, ATM, RAD50, ERCC4, RPA1, MLHI, and ATR genes. Somatic mutations of TP53 were found in nine cases (90%) and were the only stable mutations between the primary tumor and BMs. Disturbed pathways in BMs versus primary HGSOC constituted a complex network and included the cell cycle, the degradation of the extracellular matrix, cell junction organization, nucleotide metabolism, lipid metabolism, the immune system, G-protein-coupled receptors, intracellular vesicular transport, and reaction to chemical stimuli (Golgi vesicle transport and olfactory signaling). Pathway analysis approaches allow for a more intuitive interpretation of the data as compared to considering single-gene aberrations and provide an opportunity to identify clinically informative alterations in HGSOC BM.publishedVersio

microRNA Expression Profile in Single Hormone Receptor-Positive Breast Cancers Is Mainly Dependent on HER2 Status—A Pilot Study

Estrogen (ER) and progesterone (PgR) receptors and HER2 are crucial in the assessment of breast cancer specimens due to their prognostic and predictive significance. Single hormone receptor-positive breast cancers are less common and their clinical course is less favorable than ER(+)/PgR(+) tumors. Their molecular features, especially microRNA (miRNA) profiles, have not been investigated to date. Tumor specimens from 36 chemonaive breast cancer patients with known ER and PgR status (18 ER(+)/PgR(&minus;) and 18 ER(&minus;)/PgR(+) cases) were enrolled to the study. The expression of 829 miRNAs was evaluated with nCounter Human v3 miRNA expression Assay (NanoString). miRNAs differentiating between ER/PgR/HER2 phenotypes were selected based on fold change (FC) calculated for the mean normalized counts of each probe in compared groups. The differences were estimated with Student&rsquo;s t-test or Two-Way ANOVA (considering also the HER2 status). The results were validated using The Cancer Genome Atlas (TCGA) dataset. Following quality control of raw data, fourcases were excluded due to low sample quality, leaving 14 ER(+)/PgR(&minus;) and 18 ER(&minus;)/PgR(+) cases. After correction for multiple comparisons, we did not find miRNA signature differentiating between ER(&minus;)/PgR(+) and ER(+)/PgR(&minus;) breast cancers. However, a trend for differing expression (p-value &le; 0.05; FDR &gt; 0.2; ANOVA) in eight miRNAs was observed. The ER(+)/PgR(&minus;) group demonstrated elevated levels of four miRNAs&mdash;miR-30a-5p, miR-29c-3p, miR-141-3p and miR-423-5p&mdash;while the ER(&minus;)/PgR(+) tumors were enriched in another four miRNAs&mdash;miR-514b-5p, miR-424-5p, miR-495-3p, and miR-92a-3p. For one of the miRNAs&mdash;miR-29c-3p&mdash;the association with the ER(+)/PgR(&minus;) phenotype was confirmed in the TCGA cohort (p-value = 0.024; t-test). HER2 amplification/overexpression in the NanoString cohort was related to significant differences observed in 33 miRNA expression levels (FDR &le; 0.2; ANOVA). The association with HER2 status was confirmed in the TCGA cohort for four miRNAs (miR-1180-3p, miR-223-3p, miR-30d-5p, and miR-195-5p). The main differences in miRNA expression amongst single hormone receptor-positive tumors were identified according to their HER2 status. However, ER(+)/PgR(&minus;) cases tended to express higher levels of miRNAs associated with ER-positivity (miR-30a-5p, miR-29c-3p, miR-141-3p), whereas ER(&minus;)/PgR(+) cancers showed elevated levels of miRNAs characteristic for double- and triple-negative tumors (miR-92a-3p, miR-424-5p). Further studies are necessary to comprehensively analyze miRNA signatures characteristic of ER(&minus;)/PgR(+) and ER(+)/PgR(&minus;) tumors