CRACK PATTERN SIMULATION OF PRESSURIZED BOROSILICATE GLASS TUBE UNDER PELLET IMPACT USING ALE METHOD

Transformer bushings are a common target for sniper attacks to cause power failure in large areas. Given the size, internal pressure and brittle nature of transformer bushing, pellet impact causes damage to surrounding components due to broken bushing pieces. To mitigate this, a project was initiated by United States Burau of Reclaimation to develop a safety mechanism. Finite Element Analysis is proposed to optimize and reduce cost of the design. A simplified Finite Element model is created which consists of 0.22 caliber lead pellet impacting Pyrex 7740 borosilicate glass tube. Previous studies on the effect of the mesh pattern, size and cap geometry were carried out. One of the biggest factors which can help in mitigating the transformer component damage is internal pressure of bushing fluid. This study focuses on developing a Finite Element model which captures the fluid-structure interaction between glass tube and internal pressurized air. Arbitrary Lagrangian Eulerian code of LS-DYNA is used to simulate this interaction. This report describes the modelling techniques used to simulate the pellet impact on pressurized tube. Failure strain calculations are also discussed in the report. The effect of internal pressure and failure strain are addressed. Crack pattern and broken glass fragments size are considered for a comparison between the pellet impact simulation and experiment

Investigation of Incompressible Flow Past Two Circular Cylinders of Different Diameters

A two - dimensional Navier-Stokes solver based on finite volume approach using a boundary-fitted curvilinear structured O-grid has been developed to obtain details of unconfined flow past cylinders at low Reynolds number of 100 and 200 based on diameter. Computations made on a single cylinder with smaller domain adopting the convective boundary conditions captured most of the flow features. This concept of a smaller domain, when used to capture the highly complex flow field around two cylinders of the same diameter placed in tandem at a Reynolds number of 200 showed reasonable results. The details of the flow field around two cylinders of different diameters placed at a typical distance of 3L and Reynolds number of 100 could be well captured adopting smaller domain concept. It is observed that the change in diameter of upstream cylinder strongly influences the overall flow field and the drag of the downstream cylinder

Unexpected instabilities explain batch-to-batch variability in cell-free protein expression systems

Cell-free methods of protein synthesis offer rapid access to expressed proteins. Though the amounts produced are generally only at a small scale, these are sufficient to perform protein-protein interaction assays and tests of enzymatic activity. As such they are valuable tools for the biochemistry and bioengineering community. However the most complex, eukaryotic cell-free systems are difficult to manufacture in house and can be prohibitively expensive to obtain from commercial sources. The Leishmania tarentolae system offers a relatively cheap alternative which is capable of producing difficult to express proteins, but which is simpler to produce in large scale. However, this system suffers from batch-to-batch variability, which has been accepted as a consequence of the complexity of the extracts. Here we show an unexpected origin for the variability observed and demonstrate that small variations in a single parameter can dramatically affect expression, such that minor pipetting errors can have major effects on yields. L. tarentolae cell-free lysate activity is shown to be more stable to changes in Mg concentration at a lower ratio of feed solution to lysate in the reaction than typically used, and a higher Mg optimum. These changes essentially eliminate batch-to-batch variability of L. tarentolae lysate activity and permit their full potential to be realized

Designing chitosan-tripolyphosphate microparticles with desired size for specific pharmaceutical or forensic applications

Chitosan (CS) is a natural cationic polymer obtained by the partial N-deacetylation of chitin. Chitosan microparticles can be prepared by cross-linking with tripolyphosphate (TPP) via the ionic interaction between positively charged amino groups (CS) and negatively charged counter ions (TPP). This can be controlled by the charge density of CS and TPP, which depend on the pH and ionic strength of the solution. The purpose of this study is to investigate the combined effects of three independent variables (pH, ionic strength and CS: TPP ratio) on three important physico-chemical properties (viscosity, zeta potential and particle size) during the preparation of microparticles. CS: TPP microparticles were prepared using experimental design and equations were generated and used to predict relative viscosity, zeta potential and particle size under different conditions. This gives us the ability to design tuneable CS-TPP microparticles with desired size for specific pharmaceutical or forensic applications e.g. latent fingerprint visualisation

Photocatalytic and biomedical investigation of green synthesized NiONPs: Toxicities and degradation pathways of Congo red dye

In this study the biomedical and catalytic ability of green synthesized nickel Oxide nanoparticles (NiONPs) was investigated. The extract of medicinal plant Tribulus terrestris were used to synthesized NiONPs. The as-synthesized NiONPs were in nano scale and were characterized with X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), High-Resolution Transmission Electron Microscopy (HRTEM), Scanning Electron Microscope (SEM), X-ray photoelectron spectroscopy (XPS) and Energy Dispersive X-ray spectroscopy (EDX). The synthesis of NiONPs was ensured with surface Plasmon resonance (SPR) at 425 nm and the strong EDX. The effect of different concentrations of plant extract on the size of NiONPs was evaluated. The HRTEM results showed particle size between 60 - 90 nm. The study showed that lesser the extract concentration, more spherical and small sized particles were obtained without aggregation. The biological applications of NiONPs were evaluated against different fungal species like Asper gillusflavus, Asper gillusfumigatus, Asper gillusniger and standard medicine Terbinafine. A. niger, A. flavus and A. fumigatus exhibited 57, 63 and 52 % inhibition compared with inhibition of the reference medicine which is 98, 100 and 100%. NiONPs have been shown to be more effective against gram-positive bacteria than gram-negative bacteria like E. Coli 9(±0.7) and S. aureus13 (±0.8). Moreover, antioxidant properties of the as-synthesized NiONPs were evaluated with 2, 2 diphenyl-1-picrylhydrazyl) (DPPH). The catalytic ability of green synthesized of NiONP was investigated for the degradation of Congo red dye (CR) as a hazardous environmentally contaminations in water. The biosynthesized NiONPs were found to be active catalytic for the degradation toxic dyes like CR. the catalytic activity of NiONPs can be explained by its small size compared with balk material. Mechanisms for CR degradation have been proposed. The Ecotoxicity of CR and components derived from dye was investigated with Ecological Structure Activity Relationship (ECOSAR) program

BACKGROUND SUBTRACTION ALGORITHM BASED ABANDONED OBJECT DETECTION

Abandoned object detection is a basic necessity in numerous video observation settings. Two background short term and long term are built and refreshed persistently continuously. Change detection Mean-ratio and Log Ratio operators can be utilized. To accomplish higher exactness combination. Movement based criteria should be connected for static and moving object detection. Proposed strategy can distinguish little deserted objects inside low quality observation recordings, and it is additionally strong to the fluctuating illuminations and dynamic background