92 research outputs found
Conjugation of gluten hydrolysates with glucosamine at mild temperatures enhances antioxidant and antimicrobial properties
Gluten represents one of the principal by-products of the wheat starch industry. Peptides obtained by wheat hydrolysis can be used for specific functional and biological activities, albeit at relatively low yields. Although the Maillard reaction (glycation) is widely used to increase functionality of proteins, its main disadvantage is the production of undesirable compounds due to high processing temperature. In this research, functional and biologically active glycopeptides were obtained from gluten. Alcalase or Flavourzyme proteases were used to hydrolyse gluten protein, and the resulting peptides were conjugated with glucosamine by enzymatic glycosylation, using transglutaminase, or through glycation. Both reactions were performed at mild temperatures (25 or 37°C). The formation of glycopeptides depended mostly on the glycation process, as demonstrated by MALDI-TOF-MS. The bioactivities of the conjugated hydrolysates were compared to the native hydrolysates. Although a reduction in the anti-ACE activity was detected, improved DPPH scavenging activity and enhanced antimicrobial activity against Escherichia coli were observed in the glycated Alcalase-derived hydrolysates and in the glycated Flavourzyme-derived hydrolysates, respectively. This study showed that mild conditions are an alternate approach to the traditional Maillard process conducted at elevated temperatures in creating conjugated gluten hydrolysates with enhanced bioactivities. © 2014 Elsevier Ltd
Covariance function modelling in local geodetic applications using the simplex method
Collocation has been widely applied in geodesy for estimating the gravity field of the Earth both locally and globally. Particularly, this is the standard geodetic method used to combine all the available data to get an integrated estimate of any functional of the anomalous potential T. The key point of the method is the definition of proper covariance functions of the data. Covariance function models have been proposed by many authors together with the related software. In this paper a new method for finding suitable covariance models has been devised. The covariance fitting problem is reduced to an optimization problem in Linear Programming and solved by using the Simplex Method. The procedure has been implemented in a FORTRAN95 software and has been tested on simulated and real data sets. These first tests proved that the proposed method is a reliable tool for estimating proper covariance function models to be used in the collocation procedure
COVARIANCE FUNCTION MODELLING IN LOCAL GEODETIC APPLICATIONS USING THE SIMPLEX METHOD
Collocation has been widely applied in geodesy for estimating the gravity field of the Earth both locally and globally. Particularly, this is the standard geodetic method used to combine all the available data to get an integrated estimate of any functional of the anomalous potential T. The key point of the method is the definition of proper covariance functions of the data. Covariance function models have been proposed by many authors together with the related software. In this paper a new method for finding suitable covariance models has been devised. The covariance fitting problem is reduced to an optimization problem in Linear Programming and solved by using the Simplex Method. The procedure has been implemented in a FORTRAN95 software and has been tested on simulated and real data sets. These first tests proved that the proposed method is a reliable tool for estimating proper covariance function models to be used in the collocation procedure
Les droits disciplinaires des fonctions publiques : « unification », « harmonisation » ou « distanciation ». A propos de la loi du 26 avril 2016 relative à la déontologie et aux droits et obligations des fonctionnaires
The production of tt‾ , W+bb‾ and W+cc‾ is studied in the forward region of proton–proton collisions collected at a centre-of-mass energy of 8 TeV by the LHCb experiment, corresponding to an integrated luminosity of 1.98±0.02 fb−1 . The W bosons are reconstructed in the decays W→ℓν , where ℓ denotes muon or electron, while the b and c quarks are reconstructed as jets. All measured cross-sections are in agreement with next-to-leading-order Standard Model predictions.The production of , and is studied in the forward region of proton-proton collisions collected at a centre-of-mass energy of 8 TeV by the LHCb experiment, corresponding to an integrated luminosity of 1.98 0.02 \mbox{fb}^{-1}. The bosons are reconstructed in the decays , where denotes muon or electron, while the and quarks are reconstructed as jets. All measured cross-sections are in agreement with next-to-leading-order Standard Model predictions
Measurement of the J/ψ pair production cross-section in pp collisions at TeV
The production cross-section of J/ψ pairs is measured using a data sample of pp collisions collected by the LHCb experiment at a centre-of-mass energy of TeV, corresponding to an integrated luminosity of 279 ±11 pb. The measurement is performed for J/ψ mesons with a transverse momentum of less than 10 GeV/c in the rapidity range 2.0 < y < 4.5. The production cross-section is measured to be 15.2 ± 1.0 ± 0.9 nb. The first uncertainty is statistical, and the second is systematic. The differential cross-sections as functions of several kinematic variables of the J/ψ pair are measured and compared to theoretical predictions.The production cross-section of pairs is measured using a data sample of collisions collected by the LHCb experiment at a centre-of-mass energy of , corresponding to an integrated luminosity of . The measurement is performed for mesons with a transverse momentum of less than in the rapidity range . The production cross-section is measured to be . The first uncertainty is statistical, and the second is systematic. The differential cross-sections as functions of several kinematic variables of the pair are measured and compared to theoretical predictions
Measurement of forward production in collisions at TeV
A measurement of the cross-section for production in collisions is presented using data corresponding to an integrated luminosity of fb collected by the LHCb experiment at a centre-of-mass energy of TeV. The electrons are required to have more than GeV of transverse momentum and to lie between 2.00 and 4.25 in pseudorapidity. The inclusive production cross-sections, where the decays to , are measured to be \begin{align*} \begin{split} \sigma_{W^{+} \to e^{+}\nu_{e}}&=1124.4\pm 2.1\pm 21.5\pm 11.2\pm 13.0\,\mathrm{pb},\\ \sigma_{W^{-} \to e^{-}\bar{\nu}_{e}}&=\,\,\,809.0\pm 1.9\pm 18.1\pm\,\,\,7.0\pm \phantom{0}9.4\,\mathrm{pb}, \end{split} \end{align*} where the first uncertainties are statistical, the second are systematic, the third are due to the knowledge of the LHC beam energy and the fourth are due to the luminosity determination. Differential cross-sections as a function of the electron pseudorapidity are measured. The cross-section ratio and production charge asymmetry are also reported. Results are compared with theoretical predictions at next-to-next-to-leading order in perturbative quantum chromodynamics. Finally, in a precise test of lepton universality, the ratio of boson branching fractions is determined to be \begin{align*} \begin{split} \mathcal{B}(W \to e\nu)/\mathcal{B}(W \to \mu\nu)=1.020\pm 0.002\pm 0.019, \end{split} \end{align*} where the first uncertainty is statistical and the second is systematic.A measurement of the cross-section for production in collisions is presented using data corresponding to an integrated luminosity of fb collected by the LHCb experiment at a centre-of-mass energy of TeV. The electrons are required to have more than GeV of transverse momentum and to lie between 2.00 and 4.25 in pseudorapidity. The inclusive production cross-sections, where the decays to , are measured to be \begin{equation*} \sigma_{W^{+} \to e^{+}\nu_{e}}=1124.4\pm 2.1\pm 21.5\pm 11.2\pm 13.0\,\mathrm{pb}, \end{equation*} \begin{equation*} \sigma_{W^{-} \to e^{-}\bar{\nu}_{e}}=\,\,\,809.0\pm 1.9\pm 18.1\pm\,\,\,7.0\pm \phantom{0}9.4\,\mathrm{pb}, \end{equation*} where the first uncertainties are statistical, the second are systematic, the third are due to the knowledge of the LHC beam energy and the fourth are due to the luminosity determination. Differential cross-sections as a function of the electron pseudorapidity are measured. The cross-section ratio and production charge asymmetry are also reported. Results are compared with theoretical predictions at next-to-next-to-leading order in perturbative quantum chromodynamics. Finally, in a precise test of lepton universality, the ratio of boson branching fractions is determined to be \begin{equation*} \mathcal{B}(W \to e\nu)/\mathcal{B}(W \to \mu\nu)=1.020\pm 0.002\pm 0.019, \end{equation*} where the first uncertainty is statistical and the second is systematic.A measurement of the cross-section for W → eν production in pp collisions is presented using data corresponding to an integrated luminosity of 2 fb collected by the LHCb experiment at a centre-of-mass energy of TeV. The electrons are required to have more than 20 GeV of transverse momentum and to lie between 2.00 and 4.25 in pseudorapidity. The inclusive W production cross-sections, where the W decays to eν, are measured to be where the first uncertainties are statistical, the second are systematic, the third are due to the knowledge of the LHC beam energy and the fourth are due to the luminosity determination
Measurement of the B0s→μ+μ− Branching Fraction and Effective Lifetime and Search for B0→μ+μ− Decays
A search for the rare decays Bs0→μ+μ- and B0→μ+μ- is performed at the LHCb experiment using data collected in pp collisions corresponding to a total integrated luminosity of 4.4  fb-1. An excess of Bs0→μ+μ- decays is observed with a significance of 7.8 standard deviations, representing the first observation of this decay in a single experiment. The branching fraction is measured to be B(Bs0→μ+μ-)=(3.0±0.6-0.2+0.3)×10-9, where the first uncertainty is statistical and the second systematic. The first measurement of the Bs0→μ+μ- effective lifetime, τ(Bs0→μ+μ-)=2.04±0.44±0.05  ps, is reported. No significant excess of B0→μ+μ- decays is found, and a 95% confidence level upper limit, B(B0→μ+μ-)<3.4×10-10, is determined. All results are in agreement with the standard model expectations.A search for the rare decays and is performed at the LHCb experiment using data collected in collisions corresponding to a total integrated luminosity of 4.4 fb. An excess of decays is observed with a significance of 7.8 standard deviations, representing the first observation of this decay in a single experiment. The branching fraction is measured to be , where the first uncertainty is statistical and the second systematic. The first measurement of the effective lifetime, ps, is reported. No significant excess of decays is found and a 95 % confidence level upper limit, , is determined. All results are in agreement with the Standard Model expectations
Measurements of prompt charm production cross-sections in pp collisions at TeV
Production cross-sections of prompt charm mesons are measured using data from collisions at the LHC at a centre-of-mass energy of TeV. The data sample corresponds to an integrated luminosity of pb collected by the LHCb experiment. The production cross-sections of , , , and mesons are measured in bins of charm meson transverse momentum, , and rapidity, . They cover the rapidity range and transverse momentum ranges for and and for and mesons. The inclusive cross-sections for the four mesons, including charge-conjugate states, within the range of are determined to be \begin{equation*} \sigma(pp\rightarrow D^0 X) = 1190 \pm 3 \pm 64\,\mu\text{b} \end{equation*} \begin{equation*} \sigma(pp\rightarrow D^+ X) = 456 \pm 3 \pm 34\,\mu\text{b} \end{equation*} \begin{equation*} \sigma(pp\rightarrow D_s^+ X) = 195 \pm 4 \pm 19\,\mu\text{b} \end{equation*} \begin{equation*} \sigma(pp\rightarrow D^{*+} X)= 467 \pm 6 \pm 40\,\mu\text{b} \end{equation*} where the uncertainties are statistical and systematic, respectively.Production cross-sections of prompt charm mesons are measured using data from pp collisions at the LHC at a centre-of-mass energy of 5 TeV. The data sample corresponds to an integrated luminosity of 8.60 ± 0.33 pb collected by the LHCb experiment. The production cross-sections of D, D, D , and D mesons are measured in bins of charm meson transverse momentum, p, and rapidity, y. They cover the rapidity range 2.0 < y < 4.5 and transverse momentum ranges 0 < p < 10 GeV/c for D and D and 1 < p < 10 GeV/c for D and D mesons. The inclusive cross-sections for the four mesons, including charge-conjugate states, within the range of 1 < p < 8 GeV/c are determined to be where the uncertainties are statistical and systematic, respectively.Production cross-sections of prompt charm mesons are measured using data from collisions at the LHC at a centre-of-mass energy of TeV. The data sample corresponds to an integrated luminosity of pb collected by the LHCb experiment. The production cross-sections of , , , and mesons are measured in bins of charm meson transverse momentum, , and rapidity, . They cover the rapidity range and transverse momentum ranges for and and for and mesons. The inclusive cross-sections for the four mesons, including charge-conjugate states, within the range of are determined to be \sigma(pp\rightarrow D^0 X) = 1004 \pm 3 \pm 54\,\mu\text{b} \sigma(pp\rightarrow D^+ X) = 402 \pm 2 \pm 30\,\mu\text{b} \sigma(pp\rightarrow D_s^+ X) = 170 \pm 4 \pm 16\,\mu\text{b} \sigma(pp\rightarrow D^{*+} X)= 421 \pm 5 \pm 36\,\mu\text{b} where the uncertainties are statistical and systematic, respectively
Both PepT1 and GLUT Intestinal Transporters Are Utilized by a Novel Glycopeptide Pro-Hyp-CONH-GlcN
Pro-Hyp
(PO) accounts for many beneficial biological effects of
collagen hydrolysates for skin and bone health. The objective of this
study was to conjugate PO with glucosamine (GlcN) to create a novel
glycopeptide Pro-Hyp-CONH-GlcN (POGlcN) and then to investigate the
potential involvement of multiple transepithelial transport pathways
for this glycopeptide. Nuclear magnetic resonance results revealed
the amide nature of this glycopeptide with α and β configurations
derived from GlcN. This glycopeptide was very resistant to simulated
gastrointestinal digestion. Also, it showed a rate of transepithelial
transport [permeability coefficient (<i>P</i><sub>app</sub>) of (2.82 ± 0.15) × 10<sup>–6</sup> cm/s] across
the Caco-2 cell monolayer superior to those of parental dipeptide
PO and GlcN [<i>P</i><sub>app</sub> values of (1.45 ±
0.17) × 10<sup>–6</sup> and (1.87 ± 0.15) ×
10<sup>–6</sup> cm/s, respectively]. A transport mechanism
experiment indicated that the improved transport efficiency of POGlcN
is attributed to the introduction of glucose transporters
Glucosamine-induced glycation of hydrolysed meat proteins in the presence or absence of transglutaminase: Chemical modifications and taste-enhancing activity
Salt reduction in food is a challenging task. The food processing sector has adopted taste enhancers to replace salt partially. In this study, a flavour enhancer formulation (liquid seasoning) was produced using enzymatically hydrolysed poultry proteins isolate (PPI). The PPI obtained through the isoelectric solubilisation precipitation process (ISP) was hydrolysed with Alcalase and glycated with glucosamine (GlcN) at moderate temperatures (37/50 �C) in the presence or absence of transglutaminase (TGase). The glycated hydrolysates showed reduced fluorescence advanced glycated end-products (AGE) and a reduced amount of alpha-dicarbonyl compounds (a-DC). An untrained consumer panel ranked the meat protein hydrolysate seasoning saltier than the salty standard seasoning solution (p < 0.05) regardless of GlcN glycation (both tested at 0.3 M Na+). GlcN treatments showed a tendency (p = 0.0593) to increase savouriness. Free glutamic acid and free aspartic acid found in the PPI hydrolysate likely increased the salty perception
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