Relatório de actividade profissional (ao abrigo da recomendação do CRUP)

Relatório de Actividade Profissional ao abrigo da Recomendação do CRUP para cumprimento dos requisitos necessários à obtenção do grau de Mestre em Gestão do Território área de especialização em Detecção Remota e Sistemas de Informação GeográficaOs SIG constituem o suporte informativo, técnico e metodológico necessário à gestão do conhecimento geográfico e consequente criação de valor e de vantagens competitivas nas organizações, promovem a agilização e simplificação de procedimentos administrativos, conduzindo a ganhos de produtividade, ao aumento da eficácia, eficiência e transparência no processo de decisão. São um instrumento para a modernização administrativa e para o exercício da Cidadania, contribuindo para o reforço da competitividade, inovação e coesão territorial. No contexto deste relatório de actividade profissional, é possível comprovar isso mesmo, bem como o papel da proponente nas várias organizações onde desempenhou funções, demonstrando-se o claro enquadramento do seu percurso profissional no âmbito do Mestrado em Gestão do Território – Área de Especialidade em Detecção Remota e Sistemas de Informação Geográfica

Da conversão do contrato de trabalho a termo

Introduction; 1. Systematization of fixed term contract in the portuguese Labour Code; 2. Ancillary provisions of condition and term; 3. Article 147 of the portuguese Labour Code; 4. Conversion of the fixed-term employment contract into an open-ended contract; 5. Situations in which the employment contract is considered to have no end in the light of article 147, paragraph 1 of the Labour Code; 6. When the fixed-term employment contract becomes an open-ended contract in the light of paragraph 2 of article 147 of the Labour Code; Conclusions; References.Introducción; 1. Sistematización del contrato de trabajo de duración determinada en el Código del Trabajo portugués; 2. Disposiciones accesorias de condición y plazo; 3. Artículo 147 del Código del Trabajo portugués; 4. Conversión del contrato de trabajo de duración determinada en contrato indefinido; 5. Situaciones en las que se considera que el contrato de trabajo no tiene fin a la luz del artículo 147, párrafo 1 del Código del Trabajo; 6. Cuando el contrato de trabajo de duración determinada se convierte en contrato indefinido a la luz del párrafo 2 del artículo 147 del Código del Trabajo; Conclusiones; Referencias.O mercado de trabalho português apresenta valores consideravelmente elevados no que respeita à contratação de trabalhadores a termo. O estudo desta modalidade contratual e a análise, a cada momento, da doutrina e da jurisprudência produzidas reveste-se de grande utilidade. O presente artigo tem como objeto primordial o mecanismo previsto no artigo 147.º do Código do Trabalho, relativo a situações em que o contrato de trabalho a termo se considera ou se converte num contrato de trabalho sem termo e, em especial, a análise das situações em causa e da sua natureza preventiva e sancionatória

Cidades circulares – Contributos da Economia Circular no desenvolvimento urbano sustentável

O presente Relatório tem como finalidade expor o trabalho realizado ao longo do Estágio Curricular na Direção-Geral do Território (DGT). Este estágio corresponde à Componente Não Letiva (CNL) do Mestrado em Urbanismo Sustentável e Ordenamento do Território (MUSOT), oferecido em parceria por duas unidades orgânicas da Universidade Nova de Lisboa – a Faculdade de Ciências e Tecnologia (FCT NOVA) e a Faculdade de Ciências Sociais e Humanas (NOVA FCSH). Atualmente, o sistema socioeconómico vigente na Sociedade denomina-se por Economia Linear. Este modelo traduz-se na extração elevada e pouco controlada de recursos naturais, na produção e consumo massificado de produtos e na deposição de resíduos em larga escala. Revela ser um sistema pouco autossuficiente, insustentável, tendo vindo a apresentar falhas e a provocar constrangimentos em áreas importantes da Sociedade com repercussões negativas no ambiente e no território. Desse modo, é crucial a adoção de estratégias baseadas na Economia Circular, criando um modelo socioeconómico mais dinâmico e resiliente, apto para resolver problemas e ultrapassar obstáculos ao desenvolvimento. Para fazer frente a problemas como a escassez de recursos, alterações climáticas, pressão dos ecossistemas, consumo e produção massificado e poluição ambiental, um número considerável de Governos, organizações e entidades, têm feito esforços para alterar o paradigma atual e implementar na Sociedade estratégias e medidas circulares. Para além do trabalho realizado relativamente à temática da Economia Circular, o estágio centrou-se no apoio ao desenvolvimento da Iniciativa Nacional Cidades Circulares (InC2) promovido pela DGT e realizado com a equipa da Divisão de Desenvolvimento Territorial e Politica de Cidades (DDTPC). Esta Iniciativa tem como objetivo apoiar e capacitar os municípios e comunidades na transição para a Economia Circular e promover uma rede de cidades que possa potenciar estratégias circulares no território melhorando dessa forma o acesso a fundos nacionais e europeus para o desenvolvimento e implementação de políticas públicas. A dotação do território Português de medidas circulares poderá ser benéfico para mitigar problemas como o envelhecimento da população, os desequilíbrios socioeconómicos entre o interior e o litoral, as diferenças estruturais entre as áreas rurais e urbanas, os problemas ambientais, entre outros, que penalizam o País há muitas décadas

Multiparametric in vitro genotoxicity assessment of different variants of amorphous silica nanomaterials in rat alveolar epithelial cells

The hazard posed to human health by inhaled amorphous silica nanomaterials (aSiO2 NM) remains uncertain. Herein, we assessed the cyto- and genotoxicity of aSiO2 NM variants covering different sizes (7, 15, and 40 nm) and surface modifications (unmodified, phosphonate-, amino- and trimethylsilyl-modified) on rat alveolar epithelial (RLE-6TN) cells. Cytotoxicity was evaluated at 24 h after exposure to the aSiO2 NM variants by the lactate dehydrogenase (LDH) release and WST-1 reduction assays, while genotoxicity was assessed using different endpoints: DNA damage (single- and double-strand breaks [SSB and DSB]) by the comet assay for all aSiO2 NM variants; cell cycle progression and γ-H2AX levels (DSB) by flow cytometry for those variants that presented higher cytotoxic and DNA damaging potential. The variants with higher surface area demonstrated a higher cytotoxic potential (SiO2_7, SiO2_15_Unmod, SiO2_15_Amino, and SiO2_15_Phospho). SiO2_40 was the only variant that induced significant DNA damage on RLE-6TN cells. On the other hand, all tested variants (SiO2_7, SiO2_15_Unmod, SiO2_15_Amino, and SiO2_40) significantly increased total γ-H2AX levels. At high concentrations (28 µg/cm2), a decrease in G0/G1 subpopulation was accompanied by a significant increase in S and G2/M sub-populations after exposure to all tested materials except for SiO2_40 which did not affect cell cycle progression. Based on the obtained data, the tested variants can be ranked for its genotoxic DNA damage potential as follows: SiO2_7 = SiO2_40 = SiO2_15_Unmod > SiO2_15_Amino. Our study supports the usefulness of multiparametric approaches to improve the understanding on NM mechanisms of action and hazard prediction.This work was supported by the Portuguese Foundation for Science and Technology (FCT) through ERA-NET SIINN project NanoToxClass (SIINN/0001/2013). This work was also supported by the NanoBioBarriers project (POCI-01-0145-FEDER-031162), co-financed by the Operational Program for Competitiveness and Internationalization (POCI) through European Regional Development Funds (FEDER/FNR); and by the Spanish Ministry of Science and Innovation: MCIN/AEI/10.13039/501100011033 (Grant PID2020-114908GA-I00); and the Ministry of Education, Culture and Sport BEAGAL18/00142 to V. Valdiglesias. F. Brandão (SFRH/BD/101060/2014) and M.J. Bessa (SFRH/BD/12046/2016) are recipients of FCT PhD scholarships. The Doctoral Program in Biomedical Sciences of the ICBAS – University of Porto offered additional funds. S. Fraga thanks FCT for funding through program DL 57/2016 – Norma transitória (Ref. DL-57/INSA-06/2018). Thanks are also due to FCT/MCTES for the financial support to EPIUnit (UIDB/04750/2020) and ITR (LA/P/0064/2020).info:eu-repo/semantics/publishedVersio

Evaluation of the cytotoxicity (HepG2) and chemical composition of polar extracts from the ruderal species Coleostephus myconis (L.) Rchb.f.

Coleostephus myconis (L.) Rchb.f. (Asteraceae) is a highly disseminated plant species with ruderal and persistent growth. Owing to its advantageous agronomic properties, C. myconis might have industrial applications. However, this species needs to be comprehensively characterized before any potential use. In a previous study, the phenolic composition and antioxidant activity of different C. myconis tissues were characterized. This investigation was extended to examine the cytotoxic potential of selected plant tissues (flowers and green parts) using a HepG2 cell line by utilizing the lysosomal neutral red uptake assay or mitochondrial (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay. In addition, the macronutrients content, lipophilic compounds (fatty acids, tocopherols), and amino acids were also determined. C. myconis flowers were used in the senescence stage, which was previously identified as the stage that presented maximal phenolic content and highest antioxidant activity. In contrast, stems and leaves were employed due to their high biomass proportion. Regarding cytotoxicity, mitochondrial and lysosomal damage was only significant when HepG2 cells were exposed to the highest extract concentrations (stems and leaves, 0.9 mg/ml; senescent flowers, 0.3 mg/ml). Chemically, the senescent flowers were mostly characterized by their high levels of fat, amino acids (especially threonine), oleic acid, ß-, and ?-tocopherol, while stems and leaves contained high concentrations of carbohydrates, linolenic acid, and a-tocopherol. In general, these results provide information regarding the threshold concentrations of C. myconis extracts that might be used in different applications without toxicity hazards.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support to REQUIMTE (PEst-C/EQB/LA0006/2014) and to CIMO (UID/AGR/00690/2013). J.C.M. Barreira, Carla Costa, and Filipa B. Pimentel thank FCT, POPH-QREN, and FSE for their grants (SFRH/BPD/72802/2010, SFRH/BPD/96196/2013 and SFRH/BD/109042/2015, respectively).info:eu-repo/semantics/publishedVersio

Low-velocity impact response of auxetic seamless knits combined with non-newtonian fluids

Low-velocity impact can cause serious damage to the person or structure that is hit. The development of barriers that can absorb the energy of the impact and, therefore, protect the other side of the impact is the ideal solution for the pointed situation. Auxetic materials and shear thickening fluids are two types of technologies that have great capabilities to absorb high levels of energy when an impact happens. Accordingly, within this study, the combination of auxetic knits with shear thickening fluids by the pad-dry-cure process was investigated. It was observed that, by applying knits with auxetic patterns produced with denser materials and combined with the shear thickening fluids, high performance in terms of absorbed energy from puncture impact is obtained. The increment rates obtained are higher than 100% when comparing the structures with and without shear thickening fluids.Compete 2020 grant number 4521

Sharp Hessian estimates for fully nonlinear elliptic equations under relaxed convexity assumptions, oblique boundary conditions and applications

In this work we derive global estimates for viscosity solutions to fully nonlinear elliptic equations under relaxed structural assumptions on the governing operator which are weaker than convexity and oblique boundary conditions and under suitable assumptions on the dat. Our approach makes use of geometric tangential methods, which consists of importing "fine regularity estimates" from a limiting profile, i.e., the Recession operator, associated with the original second order one via compactness and stability procedures. As a result, we devote a special attention to the borderline scenario. In such a setting, we prove that solutions enjoy BMO type estimates for their second derivatives. In the end, as another application of our findings, we obtain Hessian estimates to obstacle type problems under oblique boundary conditions and no convexity assumptions, which may have their own mathematical interest. A density result in a suitable class of viscosity solutions will be also addressed

Assessing the in vitro toxicity of airborne (nano)particles to the human respiratory system: from basic to advanced models

ReviewSeveral studies have been conducted to address the potential adverse health risks attributed to exposure to nanoscale materials. While in vivo studies are fundamental for identifying the relation-ship between dose and occurrence of adverse effects, in vitro model systems provide important information regarding the mechanism(s) of action at the molecular level. With a special focus on exposure to inhaled (nano)particulate material toxicity assessment, this review provides an over-view of the available human respiratory models and exposure systems for in vitro testing, advan-tages, limitations, and existing investigations using models of different complexity. A brief overview of the human respiratory system, pathway and fate of inhaled (nano)particles is also presented.The work was supported by the Fundação para a Ciência e a Tecnologia [POCI-01-0145-FEDER-029651, SFRH/BPD/122112/2016,PTDC/MED‐TOX/31162/2017, DL-57/INSA06/2018,SFRH/BD/101060/2014,SFRH/BD/120646/2016, SIINN/0004/2014,UIDB/04750/2020, LA/P/0064/2020]; Spanish Ministry of Education, Culture and Sport [BEAGAL18/00142]; Spanish Ministry of Science and Innovation [PID2020-114908GA-I00].info:eu-repo/semantics/publishedVersio