La evaluación de colección de libros en soporte papel del Sistema Integrado de Documentación, UNCuyo

El presente trabajo radica en realizar un estudio de métodos de evaluación de calidad en colecciones, aplicados en bibliotecas universitarias de Iberoamérica, que se puedan adaptar a las bibliotecas de la UNCuyo. Para esto se seleccionaron países como Chile, Perú, México, España y Argentina. De este análisis surge la redacción de un documento base que servirá de guía para la construcción de una herramienta de análisis que permitirá evaluar las colecciones de libros en soporte papel de las bibliotecas que integran el SID.Fil: Bermejo, Zulma Teresita. Universidad Nacional de Cuyo. Facultad de Educación Elemental y Especial.Fil: Díaz, Matías Ezequiel. Universidad Nacional de Cuyo. Facultad de Educación Elemental y Especial.Fil: Mariano, Karen Elisabeth. Universidad Nacional de Cuyo. Facultad de Educación Elemental y Especial

Microalgae strain catalogue:A strain selection guide for microalgae users: cultivation and chemical characteristics for high added-value products (3rd Edition)

The 3rd edition of this catalogue contains information on the cultivation and composition characteristics of 37 microalgae. Each entry includes relevant links to Atlantic Area stakeholders known to have a relevant connection with each of the species listed, be it in the form of culture collections, research expertise, technology developers, or biomass producers. We invite the readers to visit and/or join the EnhanceMicroAlgae Stakeholder database: an easily accessible, visual and open access database that brings together all the European Atlantic Area players working in the microalgae sector

Microalgae strain catalogue:A strain selection guide for microalgae users: 4th edition

The 4th edition of this catalogue contains information on the cultivation and composition characteristics of 50 microalgae. Each entry includes relevant links to Atlantic Area stakeholders known to have a relevant connection with each of the species listed, be it in the form of culture collections, research expertise, technology developers, or biomass producers. We invite the readers to visit and/or join the EnhanceMicroAlgae Stakeholder database: an easily accessible, visual and open access database that brings together all the European Atlantic Area players working in the microalgae sector.This publication is part of the deliverables of the Interreg-funded international project EnhanceMicroAlgae. The authors gratefully acknowledge the European Regional Development Fund (ERDF) Interreg Atlantic Area programme which funded the EnhanceMicroAlgae project: EAPA_338/2016, "High added-value industrial opportunities for microalgae in the Atlantic Area"

Microalgae as a safe food source for animals: nutritional characteristics of the acidophilic microalga Coccomyxa onubensis

Background Edible microalgae are marine or fresh water mesophilic species. Although the harvesting of microalgae offers an abundance of opportunities to the food and pharmaceutical industries, the possibility to use extremophilic microalgae as a food source for animals is not well-documented. Objective We studied the effects of dietary supplementation of a powdered form of the acidophilic microalga Coccomyxa onubensis on growth and health parameters of laboratory rats. Method Four randomly organized groups of rats (n=6) were fed a standard diet (Diet 1, control) or with a diet in which 0.4% (Diet 2), 1.25% (Diet 3), or 6.25% (Diet 4) (w/w) of the standard diet weight was substituted with dried microalgae powder, respectively. The four groups of animals were provided ad libitum access to feed for 45 days. Results C. onubensis biomass is rich in protein (44.60% of dry weight) and dietary fiber (15.73%), and has a moderate carbohydrate content (24.8%) and a low lipid content (5.4%) in which polyunsaturated fatty acids represent 65% of the total fatty acid. Nucleic acids are present at 4.8%. No significant difference was found in growth rates or feed efficiency ratios of the four groups of rats. Histological studies of liver and kidney tissue revealed healthy organs in control and C. onubensis-fed animals, while plasma hematological and biochemical parameters were within healthy ranges for all animals. Furthermore, animals fed a microalgae-enriched diet exhibited a statistically significant decrease in both blood cholesterol and triglyceride levels. The blood triglyceride content and very low density lipoprotein-cholesterol levels decreased by about 50% in rats fed Diet 4. Conclusions These data suggest that C. onubensis may be useful as a food supplement for laboratory animals and may also serve as a nutraceutical in functional foods. In addition, microalgae powder-supplemented diets exerted a significant hypocholesterolemic and hypotriglyceridemic effect in animals

Genetic polymorphisms located in TGFB1, AGTR1, and VEGFA genes are associated to chronic renal allograft dysfunction

Background: Persistent inflammation and fibrosis have been related to active progression of renal deterioration and reduced survival of kidney transplant. The aim of this study was to determine the impact of single-nucleotide polymorphisms (SNPs) located in regions related to inflammatory and immune processes on the development of chronic renal allograft dysfunction (CRAD). Methods: A retrospective study was carried out on 276 patients who received kidney transplant (KT). SNPs were genotyped via the SNPlex platform. Statistical analysis was performed with SNPstat and regression logistic analyses were adjusted by age and gender of recipients and donors, cold ischemia time and the number of human leukocyte antigen (HLA) mismatches. Results: From 276 patients with KT, 118 were non-CRAD and 158 were CRAD. Three SNPs showed significant associations with CRAD development: rs1800471 in transforming growth factor beta 1 (TGFB1), rs5186 in angiotensin II receptor type 1 (AGTR1), and rs699947 in vascular endothelial growth factor A (VEGFA). GC genotype of rs1800471 was associated with increased odds of CRAD compared to GG genotype (OR=2.65 (95% confidence interval (CI)=1.09; 6.47), p=0.025), as well as AC and AA genotype of rs699947 assuming a dominant model (OR=1.80 (95% CI=1.02; 3.20), p=0.044). Besides, AC and CC genotypes of rs5186 were associated with reduced odds of CRAD assuming a dominant model (OR=0.56 (95% CI=0.33; 0.96), p=0.033). Conclusion: Our findings suggest that three genes related to immunity and inflammation (rs1800471, rs5186 and rs699947) are associated to susceptibility or protection to CRAD, and might have diagnostic utility in predicting the likelihood of developing CRAD.This work was supported by two Grants from Instituto de Salud Carlos III (Ref: PI08/0738 and PI11/00245) and from Junta de Castilla y León (Ref: GRS 234/A/08). MAJs, AFR and MGF are supported by Grants from Instituto de Salud Carlos III, CM10/00105, UIPY-1377/08 and CM09/00031, respectively.S

Genetic polymorphisms located in genes related to immune and inflammatory processes are associated with end-stage renal disease: a preliminary study

Background Chronic kidney disease progression has been linked to pro-inflammatory cytokines and markers of inflammation. These markers are also elevated in end-stage renal disease (ESRD), which constitutes a serious public health problem. Objective To investigate whether single nucleotide polymorphisms (SNPs) located in genes related to immune and inflammatory processes, could be associated with ESRD development. Design and methods A retrospective case-control study was carried out on 276 patients with ESRD and 288 control subjects. Forty-eight SNPs were genotyped via SNPlex platform. Logistic regression was used to assess the relationship between each sigle polymorphism and the development of ESRD. Results Four polymorphisms showed association with ESRD: rs1801275 in the interleukin 4 receptor (IL4R) gene (OR: 0.66 (95%CI=0.46-0.95); p=0.025; overdominant model), rs4586 in chemokine (C-C motif) ligand 2 (CCL2) gene (OR: 0.70 (95%CI=0.54-0.90); p=0.005; additive model), rs301640 located in an intergenic binding site for signal transducer and activator of transcription 4 (STAT4) (OR: 1.82 (95%CI=1.17-2.83); p=0.006; additive model) and rs7830 in the nitric oxide synthase 3 (NOS3) gene (OR: 1.31 (95%CI=1.01-1.71); p=0.043; additive model). After adjusting for multiple testing, results lost significance. Conclusion Our preliminary data suggest that four genetic polymorphisms located in genes related to inflammation and immune processes could help to predict the risk of developing ESRD.This work was supported by grants from Instituto de Salud Carlos III (Ref: PI08/0738 and PI11/00245) to SR and Junta de Castilla y Leon (Ref: GRS 234/A/08) to ET. 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Nat Genet. 2010, 42 (5): 376-384. 10.1038/ng.568.Chambers JC, Zhang W, Lord GM, van der Harst P, Lawlor DA, Sehmi JS, Gale DP, Wass MN, Ahmadi KR, Bakker SJ, et al: Genetic loci influencing kidney function and chronic kidney disease. Nat Genet. 2010, 42 (5): 373-375. 10.1038/ng.566.Ribases M, Ramos-Quiroga JA, Sanchez-Mora C, Bosch R, Richarte V, Palomar G, Gastaminza X, Bielsa A, Arcos-Burgos M, Muenke M, et al: Contribution of LPHN3 to the genetic susceptibility to ADHD in adulthood: a replication study. Genes Brain Behav. 2010, 10 (2): 149-157.Sole X, Guino E, Valls J, Iniesta R, Moreno V: SNPStats: a web tool for the analysis of association studies. Bioinformatics. 2006, 22 (15): 1928-1929. 10.1093/bioinformatics/btl268.Fried L, Solomon C, Shlipak M, Seliger S, Stehman-Breen C, Bleyer AJ, Chaves P, Furberg C, Kuller L, Newman A: Inflammatory and prothrombotic markers and the progression of renal disease in elderly individuals. J Am Soc Nephrol. 2004, 15 (12): 3184-3191. 10.1097/01.ASN.0000146422.45434.35.Wolkow PP, Niewczas MA, Perkins B, Ficociello LH, Lipinski B, Warram JH, Krolewski AS: Association of urinary inflammatory markers and renal decline in microalbuminuric type 1 diabetics. J Am Soc Nephrol. 2008, 19 (4): 789-797. 10.1681/ASN.2007050556.Nakamura E, Megumi Y, Kobayashi T, Kamoto T, Ishitoya S, Terachi T, Tachibana M, Matsushiro H, Habuchi T, Kakehi Y, et al: Genetic polymorphisms of the interleukin-4 receptor alpha gene are associated with an increasing risk and a poor prognosis of sporadic renal cell carcinoma in a Japanese population. Clin Cancer Res. 2002, 8 (8): 2620-2625.Burgos PI, Causey ZL, Tamhane A, Kelley JM, Brown EE, Hughes LB, Danila MI, van Everdingen A, Conn DL, Jonas BL, et al: Association of IL4R single-nucleotide polymorphisms with rheumatoid nodules in African Americans with rheumatoid arthritis. Arthritis Res Ther. 2010, 12 (3): R75-10.1186/ar2994.Tachdjian R, Mathias C, Al Khatib S, Bryce PJ, Kim HS, Blaeser F, O'Connor BD, Rzymkiewicz D, Chen A, Holtzman MJ, et al: Pathogenicity of a disease-associated human IL-4 receptor allele in experimental asthma. J Exp Med. 2009, 206 (10): 2191-2204. 10.1084/jem.20091480.Zheng G, Wang Y, Xiang SH, Tay YC, Wu H, Watson D, Coombes J, Rangan GK, Alexander SI, Harris DC: DNA vaccination with CCL2 DNA modified by the addition of an adjuvant epitope protects against "nonimmune" toxic renal injury. J Am Soc Nephrol. 2006, 17 (2): 465-474. 10.1681/ASN.2005020164.Kang YS, Lee MH, Song HK, Ko GJ, Kwon OS, Lim TK, Kim SH, Han SY, Han KH, Lee JE, et al: CCR2 antagonism improves insulin resistance, lipid metabolism, and diabetic nephropathy in type 2 diabetic mice. Kidney Int. 2010, 78 (9): 883-894. 10.1038/ki.2010.263.Dai R, Ahmed SA: MicroRNA, a new paradigm for understanding immunoregulation, inflammation, and autoimmune diseases. 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A Solve-RD ClinVar-based reanalysis of 1522 index cases from ERN-ITHACA reveals common pitfalls and misinterpretations in exome sequencing

Purpose Within the Solve-RD project (https://solve-rd.eu/), the European Reference Network for Intellectual disability, TeleHealth, Autism and Congenital Anomalies aimed to investigate whether a reanalysis of exomes from unsolved cases based on ClinVar annotations could establish additional diagnoses. We present the results of the “ClinVar low-hanging fruit” reanalysis, reasons for the failure of previous analyses, and lessons learned. Methods Data from the first 3576 exomes (1522 probands and 2054 relatives) collected from European Reference Network for Intellectual disability, TeleHealth, Autism and Congenital Anomalies was reanalyzed by the Solve-RD consortium by evaluating for the presence of single-nucleotide variant, and small insertions and deletions already reported as (likely) pathogenic in ClinVar. Variants were filtered according to frequency, genotype, and mode of inheritance and reinterpreted. Results We identified causal variants in 59 cases (3.9%), 50 of them also raised by other approaches and 9 leading to new diagnoses, highlighting interpretation challenges: variants in genes not known to be involved in human disease at the time of the first analysis, misleading genotypes, or variants undetected by local pipelines (variants in off-target regions, low quality filters, low allelic balance, or high frequency). Conclusion The “ClinVar low-hanging fruit” analysis represents an effective, fast, and easy approach to recover causal variants from exome sequencing data, herewith contributing to the reduction of the diagnostic deadlock

Twist exome capture allows for lower average sequence coverage in clinical exome sequencing

Background Exome and genome sequencing are the predominant techniques in the diagnosis and research of genetic disorders. Sufficient, uniform and reproducible/consistent sequence coverage is a main determinant for the sensitivity to detect single-nucleotide (SNVs) and copy number variants (CNVs). Here we compared the ability to obtain comprehensive exome coverage for recent exome capture kits and genome sequencing techniques. Results We compared three different widely used enrichment kits (Agilent SureSelect Human All Exon V5, Agilent SureSelect Human All Exon V7 and Twist Bioscience) as well as short-read and long-read WGS. We show that the Twist exome capture significantly improves complete coverage and coverage uniformity across coding regions compared to other exome capture kits. Twist performance is comparable to that of both short- and long-read whole genome sequencing. Additionally, we show that even at a reduced average coverage of 70× there is only minimal loss in sensitivity for SNV and CNV detection. Conclusion We conclude that exome sequencing with Twist represents a significant improvement and could be performed at lower sequence coverage compared to other exome capture techniques

Microalge : source of commercial interest compounds

Microalgae have generated a special interest in recent years because of the extraordinary potential they present. Their fast and easy growth and the ability they have to convert solar energy into biomass have led to the development of different cultivation techniques in order to obtain numerous and varied products of commercial interest. During photosynthesis, using only light and nutrients, microalgae can produce high added-value metabolites such as lipids, proteins, carotenoids, carbohydrates and hydrocarbons. The generated amounts of these metabolic products are closely related to environmental conditions, in particular, to the amount and intensity of light, temperature, pH, salinity and nutrient availability. Therefore, al! these factors have a great influence on the biochemical composition of microalgae and, eventually, on biomass production. These high value products present a wide range of applications in food, cosmetics, pharmaceutical industry, as well as in the manufacture of biopolymers. Among these compounds are those studied in this Doctoral Thesis, which were carotenoids, carbohydrates (exopolysaccharides) and hydrocarbons. From a commercial point of view, carotenoids are the most interesting photosynthetic pigments far their use as feed additives, especially as color enhancers. Of all known carotenoids, -carotene, lutein, zeaxanthin and astaxanthin are most commonly used for commercial purposes. On the other hand, the production of hydrocarbons and polysaccharides from microalgae and other plants is becoming a field of interest related to the generation of chemicals and polymers derived from renewable resources. The primary components of the major commercial polymers are derived from sugars (polyesters) and hydrocarbons (polyolefins), which are produced by certain algae. The interest in these compounds lies on their broad spectrum of application in the industry, in the current strong dependence on the availability of fossil resources, as well as on the sustainability of the production of bio-derived chemicals and polymers. This Doctoral Thesis is focused on the ability of certain microalgae to accumulate compounds with high added­ value, such as the aforementioned carotenoids, carbohydrates and hydrocarbons. Several microalgae isolated from Ria Tinto (Huelva) were studied, from which the one with the best productivity was selected for further experiments and identified as Coccomyxa onubensis. And other two races (A and B) of a microalga from a totally different environment environments with high temperatures, irradiance, dissolved metals, acid pH and lack of essential nutrients, which increases its metabolic response to the biosynthesis of antioxidant molecules, including carotenoids (P-carotene, zeaxanthin, astaxanthin and lutein), B. braunii is characterized by its slow growth and, at the same time, by being able to synthesize and accumulate large amounts of compounds as interesting as exo-polysaccharides and hydrocarbons. Thus, in the case of C. onubensis, the effect of difierent factors, such as temperatura and ultraviolet radiation A and 8, on the growth and production of carotenoids was studied. In the case of B. braunii, the optimization of the culture medium was carried out as a first approach, and commercial fertilizers were subsequently used to reduce costs and to see if it was possible to increase growth and carbohydrates and hydrocarbons productivity. The results obtained in this Thesis have contributed to obtain higher productivities of biomass and metabolites far both microalgal species.Resumen en castellano que será usado para la base de datos del Ministerio TESEO (máx. 4000 caracteres) Las microalgas han despertado en los últimos años un especial interés debido al extraordinario potencial que presentan. Su crecimiento fácil y rápido y la facultad que poseen para convertir la energía solar en biomasa han provocado el desarrollo de diferentes técnicas de cultivo para obtener numerosos y variados productos de interés comercial. Durante la fotosíntesis, utilizando sólo luz y nutrientes, las microalgas pueden producir metabolitos de alto valor añadido como son los lípidos, proteínas, carotenoides, carbohidratos e hidrocarburos. Las cantidades generadas de estos productos metabólicos están estrechamente ligadas a las condiciones ambientales, en particular, a la cantidad e intensidad de luz, la temperatura, el pH, la salinidad y la disponibilidad de nutrientes. Por tanto, el conjunto de estos factores tiene una gran influencia en la composición bioquímica de las microalgas y, en último término, en la producción de biomasa. Estos productos de alto valor presentan un amplio abanico de aplicaciones en alimentación, cosmética, industria farmacéutica, así como en la fabricación de biopolimeros. Entre dichos compuestos se encuentran los estudiados en esta Tesis Doctoral, que fueron carotenoides, carbohidratos (exopolisacáridos) e hidrocarburos. Desde un punto de vista comercial, los carotenoides son los pigmentos fotosintéticos más interesantes por su uso como aditivos en la alimentación, especialmente como potenciadores del color. De los carotenoides conocidos, el ­ caroteno, la luteina, la zeaxantina y la astaxantina son los más utilizados con propósitos comerciales. Por otro lado, la producción de hidrocarburos y polisacáridos a partir de microalgas, y otras plantas, se está convirtiendo en un campo de interés relacionado con la generación de químicos y polímeros derivados de recursos renovables. Los componentes primarios de los principales polímeros comerciales son derivados de azúcares (poliésteres) e hidrocarburos (poliolefinas), los cuales son producidos por determinadas algas. El interés de estos compuestos radica en el amplio espectro de aplicaciones que pueden tener en la industria, en la gran dependencia energética que existe para obtenerlos a nivel mundial a partir de recursos fósiles que son limitados, así como por la sostenibilidad de los procesos de producción de químicos y polímeros bio-derivados. Este trabajo de Tesis Doctoral se basa en el interés que genera la capacidad de algunas microalgas para acumular compuestos de alto valor añadido, como son los ya mencionados carotenoides, carbohidratos e hidrocarburos. Para ello, se estudiaron varias microalgas aisladas del cauce del Río Tinto en Huelva, de las cuales finalmente se seleccionó la identificada como Coccomyxa onubensis, y dos razas de otra microalga de un ambiente totalmente distinto llamada 80/Iyococcus braunii. Mientras que la primera se caracteriza por vivir en ambientes extremos con altas temperaturas, alta irradiancia, altas concentraciones de metales disueltos, pH ácido y carencia de nutrientes esenciales que le hace aumentar su respuesta metabólica hacia la biosintesis de moléculas antioxidantes, incluyendo carotenoides ( -caroteno, zeaxantina astaxantina y luteína), 8. braunii se caracteriza por un lento crecimiento acompañado por la síntesis y acumulación de compuestos de interés como los carbohidratos (exo­ polisacáridos) y los hidrocarburos. En base a estas premisas, se propuso optimizar et crecimiento y la producción de metabolitos de interés en ambas especies mediante et empleo de dos aproximaciones diferenciadas. En et caso de C. onubensis se realizó un estudio del efecto que distintos factores abióticos, como son la temperatura y la radiación ultravioleta A y B, producen en et crecimiento y producción de carotenoides. En el caso de 8. braunii se realizó primero una optimización del medio de cultivo y, posteriormente, con idea de reducir costes y ver si era posible aumentar tanto el crecimiento como la producción de metabolitos, se utilizaron como medios de cultivo fertilizantes comerciales, tanto para la raza productora de exo-polisacáridos (Raza A) como para la productora de hidrocarburos (Raza B). Fruto de estas aproximaciones ha sido la mejora en las productividades, tanto de biomasa como de los principales compuestos generados por ambas especies de mlcroalgas

Análisis sobre la violencia filio-parental. Perfil del menor infractor en Asturias.

En este trabajo se ha analizado el perfil del menor infractor que ha sido denunciado por violencia filio-parental hacia alguno de sus progenitores o cuidadores en el Principado de Asturias. Se han destacado las principales características de este tipo de violencia: características individuales, familiares, escolares, psicológicas y sociales del menor. También se ha señalado el tipo de delito cometido y la medida judicial impuesta cuyo cumplimiento se ha llevado a cabo en la Asociación Centro Trama