A conceptual framework for understanding ecosystem trade-offs and synergies, in communal rangeland systems.

Communal rangelands are a global resource of significant benefit to society through the provision of critical ecosystem goods and services such as carbon sequestration, water and livestock forage. The relative importance of the ecosystem goods and services provided by communal rangelands is driven by the social and environmental priorities of a range of different stakeholders at the local, regional and national level. Understanding the potential ecosystem service trade-offs (and synergies) is vital for making informed and inclusive decisions as part of the process of stakeholder engagement, both in goal setting as well as evaluating the appropriateness of outcomes in rangelands. However, application of trade-offs approaches to communal rangelands, has frequently been limited by a lack of adequate stakeholder engagement to help define important factors such as the diverse objectives of end users and the broader institutional and policy environments that frame them. To help address this, we propose a framework that conceptualises the links between different actors and trade-offs at three key levels, using communal rangelands in South Africa as a case study. Firstly, we explore environment trade-offs between key ecosystem services, largely determined through public sector engagement in the formulation of environmental policy. Secondly, we examine the potential for environmental policies to create community-environment trade-offs between the needs of local communities and those of society more broadly. Thirdly, we consider community trade-offs reflecting the many different social and economic priorities of people living in communal systems. We suggest that the framework will find greatest application in the initial process of determining potential ecosystem service trade-offs and associated land use scenarios with key stakeholders, and then subsequently in connecting the trade-offs back to these stakeholders, following analysis, as part of a ‘discussion support’ process. We also discuss the broader applicability of this approach to rangelands systems outside of South Africa

Validation of enzyme immunoassays via an adrenocorticotrophic stimulation test for the non-invasive quantification of stress-related hormone metabolites in naked mole-rats

DATA AVAILABILITY: Data supporting the reported results will be sent by the corresponding author upon request.SUPPLEMENTARY MATERIALS : TABLE S1: Summary of saline volume and synthetic adrenocorticotropic hormone (Synacthen® depot, Novartis, South Africa (Pty) Ltd.) administered to each individual during the ACTH challenge; TABLE S2: Summary of EIA sensitivities and intra-assay and inter-assay coefficients of variation in high- and low-value quality controls for all EIAs applied to measure immunoreactive urine glucocorticoid metabolite (uGCM) and fecal glucocorticoid metabolite in naked mole-rats.Small size in mammals usually restricts long-term, frequent monitoring of endocrine function using plasma as a matrix. Thus, the non-invasive monitoring of hormone metabolite concentrations in excreta may provide an invaluable approach. The aim of the current study was to examine the suitability of enzyme immunoassays (EIAs) for monitoring responses to stressors in the naked mole-rat (Heterocephalus glaber, NMR) using urine and feces as hormone matrices. A saline control administration, and a high- and low-dose adrenocorticotropic hormone (ACTH) challenge were performed on six male and six female disperser morph NMRs. The results revealed that a 5α-pregnane-3β,11β,21-triol-20-one EIA detecting glucocorticoid metabolites (GCMs) with a 5α-3β-11β-diol structure is the most suitable assay for measuring concentrations in male urine samples, whereas an 11-oxoaetiocholanolone EIA detecting GCMs with a 5β-3α-ol-11-one structure appears the most suitable EIA for quantifying GCMs in female urine. An 11-oxoaetiocholanolone EIA detecting 11,17 dioxoandrostanes was the most suitable EIA for quantifying GCMs in the feces of both sexes. There were sex-related differences in response to the high- and low-dose ACTH challenge. We recommend using feces as a more suitable matrix for non-invasive GCM monitoring for NMRs which can be valuable when investigating housing conditions and other welfare aspects.SARChI Chair of Mammalian Behavioral Ecology and Physiology from the DST–NRF South Africa and the National Research Foundation.https://www.mdpi.com/journal/animalsMammal Research InstituteZoology and Entomolog

GPS-Prot: A web-based visualization platform for integrating host-pathogen interaction data

<p>Abstract</p> <p>Background</p> <p>The increasing availability of HIV-host interaction datasets, including both physical and genetic interactions, has created a need for software tools to integrate and visualize the data. Because these host-pathogen interactions are extensive and interactions between human proteins are found within many different databases, it is difficult to generate integrated HIV-human interaction networks.</p> <p>Results</p> <p>We have developed a web-based platform, termed GPS-Prot <url>http://www.gpsprot.org</url>, that allows for facile integration of different HIV interaction data types as well as inclusion of interactions between human proteins derived from publicly-available databases, including MINT, BioGRID and HPRD. The software has the ability to group proteins into functional modules or protein complexes, generating more intuitive network representations and also allows for the uploading of user-generated data.</p> <p>Conclusions</p> <p>GPS-Prot is a software tool that allows users to easily create comprehensive and integrated HIV-host networks. A major advantage of this platform compared to other visualization tools is its web-based format, which requires no software installation or data downloads. GPS-Prot allows novice users to quickly generate networks that combine both genetic and protein-protein interactions between HIV and its human host into a single representation. Ultimately, the platform is extendable to other host-pathogen systems.</p

Non-native vascular flora of the Arctic : Taxonomic richness, distribution and pathways

We present a comprehensive list of non-native vascular plants known from the Arctic, explore their geographic distribution, analyze the extent of naturalization and invasion among 23 subregions of the Arctic, and examine pathways of introductions. The presence of 341 non-native taxa in the Arctic was confirmed, of which 188 are naturalized in at least one of the 23 regions. A small number of taxa (11) are considered invasive; these plants are known from just three regions. In several Arctic regions there are no naturalized non-native taxa recorded and the majority of Arctic regions have a low number of naturalized taxa. Analyses of the non-native vascular plant flora identified two main biogeographic clusters within the Arctic: American and Asiatic. Among all pathways, seed contamination and transport by vehicles have contributed the most to non-native plant introduction in the Arctic.Peer reviewe

Perturbation with Intrabodies Reveals That Calpain Cleavage Is Required for Degradation of Huntingtin Exon 1

Background: Proteolytic processing of mutant huntingtin (mHtt), the protein that causes Huntington's disease (HD), is critical for mHtt toxicity and disease progression. mHtt contains several caspase and calpain cleavage sites that generate N-terminal fragments that are more toxic than full-length mHtt. Further processing is then required for the degradation of these fragments, which in turn, reduces toxicity. This unknown, secondary degradative process represents a promising therapeutic target for HD. Methodology/Principal Findings: We have used intrabodies, intracellularly expressed antibody fragments, to gain insight into the mechanism of mutant huntingtin exon 1 (mHDx-1) clearance. Happ1, an intrabody recognizing the proline-rich region of mHDx-1, reduces the level of soluble mHDx-1 by increasing clearance. While proteasome and macroautophagy inhibitors reduce turnover of mHDx-1, Happ1 is still able to reduce mHDx-1 under these conditions, indicating Happ1-accelerated mHDx-1 clearance does not rely on these processes. In contrast, a calpain inhibitor or an inhibitor of lysosomal pH block Happ1-mediated acceleration of mHDx-1 clearance. These results suggest that mHDx-1 is cleaved by calpain, likely followed by lysosomal degradation and this process regulates the turnover rate of mHDx-1. Sequence analysis identifies amino acid (AA) 15 as a potential calpain cleavage site. Calpain cleavage of recombinant mHDx-1 in vitro yields fragments of sizes corresponding to this prediction. Moreover, when the site is blocked by binding of another intrabody, V_L12.3, turnover of soluble mHDx-1 in living cells is blocked. Conclusions/Significance: These results indicate that calpain-mediated removal of the 15 N-terminal AAs is required for the degradation of mHDx-1, a finding that may have therapeutic implications